Lactobacillus delbrueckii subsp. bulgaricus KLDS 1.0207 Exerts Antimicrobial and Cytotoxic Effects in vitro and Improves Blood Biochemical Parameters in vivo Against Notable Foodborne Pathogens (original) (raw)
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A study was carried out on inhibitory effects of Lactobacillus bulgaricus and Streptococcus thermophilus on growth of some pathogens isolated from clinical specimens of pathogens selected for this study were E. coli, some species of Klebsiella, Pseudomonas, Proteus, Salmonella and Shigella isolated from stool and urine samples of patients attending State Specialist hospital, Yola. Results of agar spot revealed that Lactobacillus bulgaricus produced highest antimicrobial activity against Klebsiella spp. (3.5 mm) followed by E. coli (2.4 mm), Proteus spp. (1.9 mm) Pseudomonas sp. (1.5 mm) and Shigella sp. (1.0 mm) while Streptococcus thermophilus produced highest antimicrobial activity against Klebsiella spp. (4.2 mm) followed by E. coli (4.0 mm), Proteus spp. (2.3 mm) Pseudomonas sp. (1.8 mm) and Shigella sp. (1.6 mm). Both organisms (Lactobacillus bulgaricus and Streptococcus thermophilus) may be used as probiotics when preceded by further studies. [Dilli, Zainab Hassan; De, Nandita; Sudi, Ismaila Yada and Ali-Dunkrah, Umaru. A Study on Inhibitory Effects of Lactobacillus bulgaricus and Streptococcus thermophilus as Probiotics on Some Clinical Pathogens. Researcher. 2010;2(11):38-41]. (ISSN: 1553-9865). http://www.sciencepub.net.
Metabolic role of lactobacilli in weight modification in humans and animals
Microbial pathogenesis, 2016
Obesity represents a worldwide public health crisis. Trials suggested that intestinal microbiota may contribute to the development of obesity and highlighted the involvement of bacteria, including Lactobacillus spp., in changes to the host metabolism. Several experiments have shown significant effects of Lactobacillus probiotics on weight modification. Lactobacillus spp. are involved in the digestion of complex carbohydrates not digested by the host in the colon and also participate in the degradation of lipids and simple sugars in the duodenum and jejunum. Moreover, Lactobacillus species survive throughout the gastrointestinal tract, as they are able to survive in the presence of bile and low pH, and produce an antimicrobial agent, allowing them to reduce the number of bacteria in the gut. Hence, Lactobacillus spp. can have a significant impact on microbiota and, consequently, on weight change. Here, we review current studies of Lactobacillus spp. involved in weight change and disc...
Aquaculture Research
This study was carried out to evaluate the use of Lactobacillus acidophilus as a biocontrol agent against some common ¢sh pathogenic bacteria (Staphylococcus xylosus, Aeromonas hydrophila gr.2 and Streptococcus agalactiae) in African cat¢sh, Clarias gariepinus. Eight treatments were designed inclusive of 10 C. gariepinus juveniles (mean weight 190 g) per tank, each in triplicate. Four groups of ¢sh were fed a diet supplemented with L. acidophilus, comprising about 3.01 Â 10 7 colony-forming units per gram of diet (the probiotics diet), while the other four groups were fed a diet not supplemented with probiotics (the non-probiotics diet). In the ¢rst group, ¢sh were injected with 1mL physiological saline and fed the non-probiotic diet (non-probiotic control); in the second, third and fourth groups, ¢sh were injected with 1mL each of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the non-probiotic diet (designated as non-probiotic treatments; NPsx, NPah and NPsa respectively). In the ¢fth group, ¢sh were injected with 1mL physiological saline but fed the probiotic diet (probiotic control), while ¢sh in the sixth, seventh and eighth groups were each injected with 1mL of S. xylosus, A. hydrophila gr.2 and S. agalactiae, respectively, and were all fed the probiotic diet (and designated as probiotic treatments; Psx, Pah and Psa respectively). Blood samples were collected for haematology analysis, while samples of the liver and kidney were examined for pathohistology after 7 and 21 days of infection. The results showed that the haematology parameters, packed cell volume, haemoglobin, erythrocyte sedimentation rate, red blood cell, white blood cell, total serum protein, Mg 21 , Ca 21 , Cl À , glucose, cholesterol and total immunoglobulin concentrations and the pathohistology of the liver and kidney were better in the challenged ¢sh (infected) maintained on the probiotic diet than those in the groups fed the non-probiotic diet. It is concluded, based on these results, that L. acidophilus is useful as a probiotic agent in C. gariepinus against these pathogenic bacteria (S. xylosus, A. hydrophila gr.2 and S. agalactiae). , 42, 196^209 L. acidophilus as a probiotic in African cat¢sh M A Al-Dohail et al.
Use of Lactobacillus to prevent infection by pathogenic bacteria
2002
This review focuses on the use and potential of Lactobacillus to prevent infections of the urogenital and intestinal tracts. The presence and dominance of Lactobacillus in the vagina is associated with a reduced risk of bacterial vaginosis and urinary tract infections. The mechanisms appear to involve anti-adhesion factors, by-products such as hydrogen peroxide and bacteriocins lethal to pathogens, and perhaps immune modulation or signaling effects. The instillation of Lactobacillus GR-1 and B-54 or RC-14 strains into the vagina has been shown to reduce the risk of urinary tract infections, and improve the maintenance of a normal flora. Ingestion of these strains into the gut has also been shown to modify the vaginal flora to a more healthy state. In addition, these strains inhibit the growth of intestinal, as well as urogenital pathogens, colonize the gut and protect against infections as shown in mice. Other probiotic strains, such as Lactobacillus GG, have been shown to prevent and treat gastroenteritis caused by rotavirus and bacteria. Given that lactobacilli are not the dominant commensals in a gut which comprises around 10 10 organisms, much work is still needed to define the mechanisms whereby GR-1, RC-14, GG and other strains contribute to health restoration and maintenance. Such critically important studies will require the medical science community to show a willingness to turn away from pharmaceutical remedies as the only solution to health and disease.
2020
Children overweight and obes are adorable circumstances but behind it save health problems until the moment of advanced growth and development. Lactobacillus casei strain Shirota is a functional food that is one of the types of probiotics. Some of the results of the study related to this type of probiotics is to lose weight and cholesterol levels in both animals and humans. The purpose of the study was to find out the impact of L. casei strains of Shirota on cholesterol levels and weight of obese and overweight children. This type of research is an experiment with pre-treatment design of only one sample. Data analysis using free sample t 2 test and Wilcoxon test. The results showed that the use of L. casei strain Shirota increased the weight of obese and overweight by 42,535±,10,607 kg to 44,905±13,896 kg (p = 0.00 8) and lowered total cholesterol levels by 179.05±33,763 mg/dl to 145.05±27,204 mg/dl (p = 0.000). The conclusion based on the results of this study is an intervention to...
PLOS ONE
Coronary heart disease (CHD) is one of the major causes of death and disability in industrialised countries, with elevated blood cholesterol an established risk factor. Total plasma cholesterol reduction in populations suffering from primary hypercholesterolemia may lower CHD incidence. This study investigated the cholesterol reducing capacity of Lactobacillus plantarum ECGC 13110402, a strain selected for its high bile salt hydrolase activity, in 49 normal to mildly hypercholesterolaemic adults. Primary efficacy outcomes included effect on blood lipids (total cholesterol (TC), low density lipoproteins (LDL-C), high density lipoproteins (HDL-C) and triacylgycerides (TAG), inflammatory biomarkers and occurrence/severity of gastrointestinal side effects to establish safety and tolerance of the intervention. Secondary outcomes included blood pressure, immune biomarkers, gut microbiota characterisation and metabonome changes. The study was run in a parallel, double blind, placebo controlled, randomised design in which the active group ingested 2x10 9 CFU encapsulated Lactobacillus plantarum ECGC 13110402 twice daily. Daily ingestion of the active treatment resulted in a statistically significant reduction in LDL-C in volunteers with baseline TC<5mM during the 0-12 week period (13.9%, P = 0.030), a significant reduction in TC in volunteers with baseline TC!6mM in the 0-6 week period (37.6%, P = 0.045), a significant decrease in TAG (53.9% P = 0.030) and an increase in HDL-C (14.7%, P = 0.007) in the over 60 years population in the 6-12 week period. A statistically significant reduction in systolic blood pressure was also observed across the active study group in the 6-12-week period (6.6%, P = 0.003). No impact on gastrointestinal function and side effects was observed during the study. Similar to blood and urine metabonomic analyses, faecal metagenomics did not reveal significant changes upon active or placebo intake. The results of this study suggest that Lactobacillus plantarum
International Journal of Enteric Pathogens, 2014
Background: Probiotics are live microbial supplements which can improve the healthy intestinal microbial balance. Lactobacilli are a group of lactic acid producing bacteria (LAB) that are known as natural probiotics found in the dairy products. Objectives: In this study, we aimed to detect the most potent Lactobacillus isolates of the Fars province local dairy products in cholesterol removal and investigate their antibacterial properties against some gastrointestinal pathogens. Materials and Methods: Fifteen locally produced yogurt samples of the Fars province were collected and characterized with routine microbiology methods. Cholesterol removal ability of the Lactobacilli isolates were determined, and their growth inhibitory effect on some standard pathogenic strains pathogen was evaluated using the well-diffusion method. Results: In this study, five common strains of Lactobacilli including L. acidophilus, L. casei, L. fermentum, L. lactis, and L. bulgaricus were identified in the samples obtained from the locally produced yogurt in the Fars province. L. lactis and L. acidophilus were determined as the two most active strains with the maximum rate of cholesterol assimilation (5.6 and 4.5 mg/mL, respectively) in the process of cholesterol removal. In the antibacterial activity assay, the two mentioned strains had significant inhibitory effect on all of the tested bacteria except for B. subtilis. Conclusions: Cholesterol removal ability had a direct relation with bacterial growth, so it is suggested to use the probiotic bacteria in the growth phase to achieve better results.
Beneficial Microbes
Probiotics may have potential in reducing cardiovascular disease (CVD) risk in middle-aged persons with borderline metabolic indices. The ability of potential probiotic Lactiplantibacillus plantarum Inducia to reduce CVD risk factors in persons with variable cholesterol and body mass indices (BMI) was assessed. In two parallel-armed double-blind placebo-controlled interventions (n=136) and (n=104), participants daily received either test yoghurt (Inducia) or placebo yoghurt. BMI, blood pressure, plasma glucose, cholesterol, high-sensitivity C-reactive protein (hs-CRP), oxidative stress and immunological markers were measured. Total counts of lactobacilli and L. plantarum Inducia were evaluated using real-time PCR. Significant reduction of total cholesterol, low density lipoprotein cholesterol (LDL-c) and non-high-density cholesterol occurred in both trials. The change in cholesterol (P=0.023) in persons with normal BMI and borderline cholesterol levels after four weeks of yoghurt co...
2018
Overweight and obesity are considered as a major cause of various conditions related to metabolic syndrome. Yet, considering the complex interacting factors leading to pathogenicity and underlying mechanisms, it remains a poorly defined area. Some probiotics have a reputation of a relatively long history of safe use, and an increasing number of studies are confirming benefits including anti-obesity effects when administered in adequate amounts. Recent reports demonstrate that probiotic functions may widely differ with reference to either intra-species or inter-species related data. Such differences do not necessarily reflect or explain strain specific functions of a probiotic, and thus require further assessment at the intra-species level. Various anti-obesity clinical trials with probiotics have shown discrepant results and require more consolidated studies in order to clarify the correct dose of application for reliable and constant efficacy over a long period. In this study three different strains of Lactobacillus sakei were administered in a high fat diet induced obese murine model using three different doses, 1x10 10 CFU, 1x10 9 CFU and 1x10 8 CFU, respectively, per day. Changes in body and organ weight were monitored, and serum chemistry analysis was performed for monitoring obesity associated biomarkers. The results show that only one strain of L. sakei (CJLS03) induced a dose dependent anti-obesity effect, while no correlation with either dose or body and adipose tissue weight loss could be detected for the other two L. sakei strains (L338 and L446). The body weight reduction mainly correlated with adipose tissue and obesity associated serum biomarkers such as triglycerides. This study suggests that anti-obesity effects of probiotics may vary in a strain and dose specific manner.