Phytochemical Analysis and Antioxidant and Anti-Inflammatory Capacity of the Extracts of Fruits of the Sechium Hybrid (original) (raw)
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Bioactive compounds and antioxidant capacity of fruit on Sechium edule
2010
Vegetables and fruits contain bioactive compounds with antioxidant activity such as phenols and flavonoids, carotenoids, ascorbate, alimentary fiber, selenium and many substances with antiatherogenics and anticarcinogenics properties. The purpose of this work is to assess some bioactive constituents and the antioxidant activity of the Sechium edule (Jacq) Swartz (Cucurbitaceae) fruit. Total phenol content was higher (p <0,001) in pulp (Sep):124.83mg/100g than seed (Ses): 86.36mg/100g. The antioxidant activity were estimated by: nitric oxide scavenging activity (NO), DPPH radicalscavenging activity (DPPH) and β-carotene bleaching method (β-carotene). The values expressed as percentage (%) corresponds to Ses and Sep respectively: NO 47.51 ± 1.9 and 55.62 ± 1.3; DPPH: 88.87 ± 1.1 and 89.13 ± 1.5 and β-carotene, 63.32 ± 1.5 and 53.15 ± 1.4. The studied samples have important radical scavenging activity and inhibited lipid peroxidation in vitro. We concluded that the present study pro...
Journal of chemical and pharmaceutical research, 2015
The objectives of this research were to study antio xidant activities from various fruit extracts of th ree organs of S. edule using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPR AC (Cupric ion Reducing Antioxidant Capacity) and corr elation of their total flavonoid, phenolic and caro tenoid content with IC50 of DPPH antioxidant activities and EC 50 of CUPRAC capacities. Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporat or. Antioxidant capacities were tested using DPPH and C UPRAC assays. Determination of total phenolic, flav onoid and carotenoid content was performed by spectropho tometer UV-visible and their correlation with IC 50 of DPPH scavenging capacities and EC 50 of CUPRAC capacities were analyzed by Pearson’s me thod. Ethyl acetate pedicel extract of S. edule (PD2) had the lowest IC 50 of DPPH scavenging activity 1.3 μg/ml, while ethyl acetate fruit...
International journal of pharmaceutical sciences review and research, 2023
Sechium edule, known as Chayote, belongs to the Cucurbitaceae family. It is also grown worldwide in tropical and subtropical regions. Because of its medicinal benefits, Chayote is being used as a traditional medicine in different areas of the northeast. Chayote fruit and vegetables show practical components people use in their regular diet. The current review revealed that the plant's edible parts could be used to treat different illnesses due to the presence of other secondary metabolites. The plant has also been shown to possess several pharmacological properties. The toxicological report in the present review also documented that the plant Sechium edule does not contain any neurotoxic or genotoxic metabolites, which will contribute to the toxicological information. Different parts of the plant, such as young stems and seeds, are also consumed in various forms due to the presence of proteins and vitamins. The plant has also shown high contents in aspartic, glutamic acid, alanine, proline, serine and valine which recommends that the plant can offer medicinal benefits and be used in hospital diets.
Asian Journal of Pharmaceutical and Clinical Research, 2016
Objectives: The aim of this research were to determine antioxidant activity from various fruit extracts of chayote from three different sites using two antioxidant methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and phosphomolybdenum methods, correlation of total phenolic, flavonoid, and carotenoid content in various extracts of chayote with their IC 50 of DPPH antioxidant activities and EC 50 of phosphomolybdenum capacity. Methods: An extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by ultraviolet-visible spectrophotometry and its correlation with IC Results: The lowest IC 50 50 of DPPH and EC 50 of phosphomolybdenum were analyzed by Pearson’s method. of DPPH scavenging activity was given by n-hexane fruit extract of chayote from Lembang (9.32 µg/ml), while...
Pharmaceutical biology, 2017
Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8-5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced th...
Nutrition and Cancer, 2015
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Journal of Pharmacy & Pharmacognosy Research, 2018
Context: The Sechium edule fruit is grown and consumed because of their nutritional values in different countries of Latin America. Costa Rica is one of the most important producers and exporters. It is consumed as an antioxidant because its flavonoid content suggests such activity; however, the selection of varieties to crop does not involve the phytochemical profile of them. Aims: To characterize the phytochemical and antioxidant profile of the S. edule varieties grown in Costa Rica. Methods: The in vitro radical scavenging activity of different S. edule extracts were measured using DPPH and ORAC assays, as well as their inhibition of lipid peroxidation in rat liver and their redox potential by cyclic voltammetry. Flavonoids glycosides were isolated by HPLC and their structures were determined by NMR.Results: The S. edule varieties grown in Costa Rica showed a good radical scavenging activity and inhibition of lipid peroxidation; there were significant differences between varieties. In addition, the redox potential determined by electrochemical methods suggested the presence of flavonoids, which was confirmed by the isolation of apigenin 7-O-rutinoside and luteolin 7-O-rutinoside. Conclusions: The antioxidant potential of varieties of S. edule grown in Costa Rica was confirmed, identifying the 845 variety as the best antioxidant profile.
The purpose of this study was to find out if the ethanolic fruit extract of Sechium edule fruits could prevent Imquimod (IMQ)-induced psoriasis-like dermatitis in male Wistar rats. The rats were divided into four groups of five rats each group. Group 1 served as a negative control, while groups 2 and 4 received 5 percent IMQ cream topically on shaved backs, topical 5 percent IMQ cream + S. edule (200mg/kg) orally once daily and topical 5 percent IMQ cream + S. edule (400mg/kg) orally once daily, respectively. From days 3 to 9, the animals treated with IMQ developed characteristic erythmea, scaling and thickening, according to the findings. Furthermore, skin thickness and the psoriasis area severity index (PASI) both were increased significantly. In IMQ-challenged mice, histological investigation revealed epidermal cuticle, including parakeratosis, acanthosis and perivascular infiltration of inflammatory cells. In IMQchallenged rats, treatment with S. edule (200 and 400mg/kg) significantly reversed all of these symptoms.
Pharmacognosy Communications
Introduction: Santolina chamaecyparissus L. is a small medicinal herb, cultivated in Europe, Asia and Africa due to the antihelmintic, antiseptic, anti spasmodic, bactericidal, fungicidal, digestive and vulnerary properties. Despite this, S. chamaecyparissus aerial part extractions have not been examined for antioxidant, antihemolytic and anti-inflammatory properties. Methods: S. chamaecyparissus aerial parts were extracted with solvents of varying polarity: methanol (crude) extract (CrE) chloroform extract (CHE), ethyl acetate extract (EAE), and aqueous extract (AE). The content of total phenolics, and flavonoids in all the extracts were determined with spectrophotometric methods. Both enzymatic and non-enzymatic methods were used to evaluate the antioxidant activity of the extracts. Antioxidant activity of all the extracts were investigated using free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl, DPPH • and 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulfonic acid (ABTS) assay), capacity of the inhibition of linoleic acid peroxidation (β-carotene assay), chelation of metals (iron chelating assay) and inhibition of xanthine oxidase (XO) activity. To investigate antihemolytic activity of the extracts, the 2, 2,-azobis (2-amidinopropane) dihydrochloride (AAPH) assay was used to induce erythrocyte oxidative hemolysis. An in vivo approach was carried out on mice treated with the methanol extract at a dose of 100 mg/kg/day for 21 consecutive days, and one group was treated with vitamin C (vitamin C 50 mg/kg) as a standard drug. To determine the improvement of antioxidant potential, basic biochemical parameters were used in tissue (liver), plasma and whole blood. Phorbol 12-myristate 13-acetate (PMA)-induced ear edema was used to investigate the antiinflammatory activity of methanolic extract in mice. Results: Among all the extracts analyzed, the EAE exhibited a higher phenolic and flavonoids content (373.83 ± 0.23 mg gallic acid/g, and 61.51±7.86 mg quercetin/g respectively), followed by CE and CHE. DPPH and ABTS scavenging assays showed that EAE exhibited the highest effect in the both assays, with an IC 50 of 0.01 mg/ml and 0.0085 mg/ml respectively. All extracts moderately inhibited linoleic acid oxidation, with 57 % inhibition. The CE had a considerable chelating activity on ferrous iron (IC 50 = 0.32 mg/ml). In the enzymatic method by xanthine oxidase, results demonstrated that EAE had the highest XO inhibitory effect on both XO activity and Cyt-c reduction with IC50= 0.052±0.0003 mg/ml and 0.057±0.0006 mg/ml, respectively followed by CHE and CE. In the cellular system, CrE and CHE showed a hemolysis effect with % hemolysis (62.81% ± 1.43), (62.71% ± 1.01). However, EAE provided protection against AAPH-induced hemolysis with 53.67% ± 0.97. The in vivo assay exhibited a significant decrease (79.56%) of the content of malondialdehyde (MDA) in the liver and increased glutathione (GSH) and catalase (71.95% and 59.16% respectively). The methanol extract clearly demonstrated anti-inflammatory effects by reduced ear edema induced by PMA with 61.51%. Conclusion: Our results indicate that the S. chamaecyparissus extracts (SCE) possesses potent antioxidant and anti-inflammatory properties and may be a valuable natural source that could be applicable to both the medical and food industries.