ANTIOXIDANT ACTIVITY, TOTAL PHENOLIC, AND FLAVONOID CONTENTS OF THE EXTRACT OF ENDOPHYTIC FUNGI DERIVED FROM TURMERIC (Curcuma longa) LEAVES (original) (raw)
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Asian Journal of Pharmaceutical and Clinical Research, 2020
Objective: The aim of this study is to evaluate the antioxidant potential, determination of total phenolic and flavonoid content in nine selected medicinal plants Spondias pinnata, Melia azedarach, Ageratina adenophora, Urtica dioica, Curcuma longa, Bauhinia variegata, Elaeocarpus angustifolius Blume, Achyranthes aspera, and Psidium guajava from Kavre district of Nepal using in vitro studies. Methods: Methanolic plant extracts were prepared by cold percolation method. The methanol extract of nine medicinal plants collected from Kavre district of Nepal, was screened for assessing bioactive phytoconstituents followed by antioxidant property, total phenolic, and flavonoid content. Different plants collected were powdered and extracted with methanol, concentrated by a rotatory evaporator and analyzed for the presence of phytochemicals. The antioxidant potential of the plant extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Results: The phytochemical analysis of methanolic extracts of all nine medicinal plants displayed the presence of various secondary metabolites such as alkaloids, flavonoids, polyphenols, saponins, and quinones. The extract of S. pinnata showed the highest percentage of radical scavenging activity up to 87.94±1.88 with 50% inhibitory concentration (IC50) 17.51±1.27 μg/mL, followed by B. variegata, 80.63±1.06 with IC50 value 26.55±2.61 μg/mL. The standard, ascorbic acid has IC50 value of 20.13±1.17 μg/mL. Further, the ethyl acetate fraction of S. pinnata showed the maximum percentage of radical scavenging (85.92±1.37) with IC50 value of 46.95±1.17 μg/mL. Moreover, S. pinnata displayed the highest total phenolic content (TPC) 48.26±1.23 mg GAE/g (milligram gallic acid equivalent per gram) extract while the highest flavonoid content was displayed by Melia azedarach 41.07±1.53 mg QE/g (milligram quercetin equivalent per gram) extract measured by the Folin–Ciocalteu reagent method and aluminum chloride colorimetric method. Conclusions: The preliminary results of this study have put forward the extract of S. pinnata showed the highest percentage of radical scavenging activity and S. pinnata displayed the highest TPC while the highest flavonoid content was displayed by Melia azedarach methanolic extracts although the further studies are needed to assess its mechanism of action
Tropical Journal of Pharmaceutical Research, 2016
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Preliminary Phytochemical Analysis and in Vitro Antioxidant Activity of Boscia arabica Leaves
International journal of pharmaceutical sciences review and research, 2021
Boscia arabica (Capparaceae), popularly known as "Sarh", is a plant utilized in Yemen in traditional medicine for treatment of many ailments, but very few studies have been carried out on this plant. The Boscia arabica leaves were selected to phytochemical analysis and evaluate antioxidant activity. Physico-chemical parameters of leaves powder were established and are within the acceptable ranges. The leaves powder was treated with various reagents and the mixture was observed under day light and under UV light to see the type of fluorescence. Different fluorescence colours were observed. Chemical analysis was carried out to determine the major chemical classes, phytoconstituents like carbohydrates, saponins, flavonoids, triterpenes, sterols and alkaloids were identified in studied extracts. The presence of phytoconstituents was further confirmed by thin layer chromatography and Rf values of developed spots were calculated with color intensity. Antioxidant activity of Boscia arabica leaf extracts was carried out via DPPH staining technique. The result of dot-blot assay showed yellow-coloured spots when stained with DPPH solution. The free radical scavenging activity of 80% methanol extract and it's fractions was determined and antioxidants activity ranged from (14.81±3.22) for methanol 80% extract to (85.31±3.12) for n-butanol fraction. Ethyl acetate (84.30±2.44) and n-butanol (85.31±3.12) are exhibited high free radical scavenging activity when compared with quercetin (99.10±4.22). The results obtained in this study support the use of Boscia arabica in traditional medicine, which may be a promising source for the development of natural antioxidants.