Molecular Characterization of Class 1, 2 and 3 Integrons in Serratia spp. Clinical Isolates in Poland – Isolation of a New Plasmid and Identification of a Gene for a Novel Fusion Protein (original) (raw)
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Antimicrobial Agents and Chemotherapy, 2002
We analyzed the role of integrons in the dissemination of antibiotic resistance in a recent multiresistant clinical isolate, Serratia marcescens SCH88050909 (SCH909). This isolate harbors three integrons, all on a 60-kb conjugative plasmid. By PCR, hybridization, and sequencing analyses, we found that integron 1 has the dfrA1 and ant ( 3 ") -Ia cassettes. The first cassette in integron 2 contains the ant ( 2 ") -Ia gene, separated from its attC site (59-base element) by a 1,971-bp insert containing a group II intron; this intron codes for a putative maturase-reverse transcriptase on the complementary strand and is the first such intron to be found associated with an integron. The attC site is followed by a novel aminoglycoside resistance gene, ant ( 3 ") -Ii-aac ( 6 ′) -IId , which has been characterized for its bifunctional ANT(3")-I and AAC(6′)-II activities. DNA sequence analysis of this fused cassette suggests that insertion and excision due to the integrase ...
Replicon typing of 71 multiresistant Serratia marcescens strains
Research in Microbiology, 1994
Repticon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon Irep) groups which can often be correlated with incompatibility (lnc} groups. We studied 71 multiresistant Serratia marceseens strains with 19 rap probes constructed from reference plasmid replicons belonging to known tnc groups. These probes are known to react with enteric bacterial plasmids, However. they did not repcesent the totality of the thirty known Inc groups. For 52 % of the studied strains, plasmids were identified and classified into groups FIB, FIC, FIIA, HI2, L/M, N, B/O, P, W, Y and Comg. Most (79 %l of the plasmid preparations hybridized with a single rep probe, and 21% hybridized with two different probes. Electrophoretie analysis of DNA suggested that double hybridization could result from the presence of either two different Inc plasmids in the same strain (e.g. S37] or one single plasmid with a multireplicon (e.g. $113).
Journal of Hospital Infection, 2004
We investigated an outbreak of Serratia marcescens in the adult intensive care unit of the University Hospital of Napoli. The outbreak involved 13 cases of infection by S. marcescens over a nine-month period and was caused by a single pulsed-field gel electrophoresis clone. The epidemic strain was multiply antibiotic resistant, producing an inducible Amp C-type betalactamase enzyme and carrying the trimethoprim-resistance gene and the adenyltransferase gene, which confers resistance to streptomycin and spectinomycin, within a class 1 integron. Antimicrobial therapy with betalactams was associated with S. marcescens acquisition in the intensive care unit.
Japanese Journal of Infectious Diseases, 2014
In this study, genetic environments of the transferable plasmid-mediated bla CTX-M-3 gene were characterized among 14 isolates of cefotaxime-resistant Serratia marcescens using PCR and BLAST DNA sequence analysis. A total of 3 types of genetic architectures in the regions surrounding this bla CTX-M-3 gene were identified. Type I architecture was characterized by the presence of a complete insertion sequence of tnpA-ISEcp1, identified as interrupting a reverse IS26 sequence in the upstream region of the bla CTX-M-3 gene. A reverse-directional orf477 fragment was located downstream of the bla CTX-M-3 gene, which was in the same direction of the mucA gene. A common region containing the orf513 element was located upstream of the mucA gene. Moreover, a copy of the 3?-CS2 element was located immediately upstream of the orf513 element. A novel complex class 1 integron was characterized by the presence of the dfrA19 gene, which was flanked by two copies of class 1 integrons. This is the first report to describe the dfrA19 gene within a novel complex class 1 integron in S. marcescens isolates from Taiwan. This novel complex class 1 integron structure was located distantly upstream of the bla CTX-M-3 gene.
Genetic analysis of the Serratia marcescens N28b O4 antigen gene cluster
Journal of bacteriology, 1999
The Serratia marcescens N28b wbbL gene has been shown to complement the rfb-50 mutation of Escherichia coli K-12 derivatives, and a wbbL mutant has been shown to be impaired in O4-antigen biosynthesis (X. Rubirés, F. Saigí, N. Piqué, N. Climent, S. Merino, S. Albertí, J. M. Tomás, and M. Regué, J. Bacteriol. 179:7581-7586, 1997). We analyzed a recombinant cosmid containing the wbbL gene by subcloning and determination of O-antigen production phenotype in E. coli DH5alpha by sodium dodecyl sulfate-polyacrylamide electrophoresis and Western blot experiments with S. marcescens O4 antiserum. The results obtained showed that a recombinant plasmid (pSUB6) containing about 10 kb of DNA insert was enough to induce O4-antigen biosynthesis. The same results were obtained when an E. coli K-12 strain with a deletion of the wb cluster was used, suggesting that the O4 wb cluster is located in pSUB6. No O4 antigen was produced when plasmid pSUB6 was introduced in a wecA mutant E. coli strain, sugg...
Plasmid, 2011
Some strains of Serratia entomophila and S. proteamaculans cause amber disease of the New Zealand grass grub Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The disease determinants of S. entomophila, are encoded on a 153,404-bp plasmid, termed pADAP for amber disease associated plasmid. The S. proteamaculans strain 143 (Sp143) exhibits an unusual pathotype, where only 60-70% of C. zealandica larvae infected with the bacterium succumb to disease. DNA sequence analysis of the Sp143 pU143 virulence associated region identified high DNA similarity to the pADAP sep virulence associated region, with DNA sequence variation in the sepA gene and the variable region of the sepC component. No pADAP anti-feeding prophage orthologue was detected in the Sp143 genome. The region of pADAP replication was cloned and found to replicate in S. entomophila but not in Escherichia coli. DNA sequence analysis of the plasmid pSG348 repA gene from the French isolate of Serratia grimesii, identified 93% DNA identity to the pADAP repA gene. A comparison of the pU143 virulence associated region with the completed pADAP nucleotide sequence is given.
Cloning and DNA sequence analysis of a bacteriocin gene of Serratia marcescens
Journal of General Microbiology, 1992
Serratia marcescens N28b synthesized and secreted a bacteriocin, with a molecular mass of 45 kDa, which was capable of inhibiting the growth of Escherichia coli. The expression of this bacteriocin was negligible unless induced with mitomycin C. The genes encoding the bacteriocin were cloned in plasmid pBR328. E. coli harbouring recombinant plasmid pBA189 or pBA289 expressed the Serratia marcescens N28b bacteriocin. The nucleotide sequence of the bss gene (Serratia marcescens N28b bacteriocin structural gene) was determined. The predicted amino acid sequence of the carboxy-terminal part of the bacteriocin 28b had a high degree of similarity to the pore-forming domains of colicins A, E1, B, N, Ia and Ib.
International Journal of Systematic Bacteriology, 1978
Seven new isolates of Serratiaplymuthica (including two from a human source) and 24 isolates resembling unclustered Serratia strain 38 (Grimont et al., J. Gen. Microbiol98:39-66, 1977) are described. Deoxyribonucleic acid relatedness studies, obtained with labeled reference deoxyribonucleic acid from s. plymuthica 392 confirm that S. plymuthica is a discrete species. Mean percent relatedness of S. plymuthica isolates to strain 392 was 88 f 13.6 (standard deviation), whereas the mean percent relatedness of S. Ziquefaciens isolates (the closest species) to strain 392 was 52 f 12.4. Strain 38 and twenty-four "38-like" isolates constitute a new deoxyribonucleic acid hybridization group that is 28 to 43% related to Serratia species and to the "Citrobacter-like" group of Leclerc and Buttiaux, and 15 to 22% related to other known species of Enterobacteriaceae. This 38-like group constitutes a new species that is named Serratia odorifera sp. nov. (type strain, ICPB 3995). Two biotypes (1 and 2) are described. Strains of this species will grow on caprylate-thallous agar (selective for Serratia spp.), and they have a characteristic odor. Most strains of S. odorifera were recovered from clinical specimens.
Iranian journal of microbiology, 2013
Nowadays, the presence of extended-spectrum β-lactamases (ESBLs) producing strains in Serratia genus causes the emergence of resistance to many antibiotics. So, the lack of proper diagnosis of ESBLs strains can lead to failure in the treatment. The objective of the present study was to investigate ESBLs production in Serratia strains isolated from the clinical blood samples in Shiraz, Iran. In this study, 39 Serratia strains isolated from the patients referred to Namazi Hospital, during a 2 year period were tested. The antimicrobial resistance of the isolates to 21 antibiotics was evaluated using Kirby-Bauer disk diffusion method. Combination disk method was used to determine the ESBL phenotype among the isolates. PCR was performed to investigate the presence of ESBL genes of SHV, OXA and TEM types. The lowest antibiotic resistance rates belonged to meropenem (7.69%) and imipenem (5.12%). Overall, positive ESBL phenotype was identified in 69% (n = 27) of the isolates, 70.37% (n = 19...