Genetic Environments of the Transferable Plasmid-Mediated blaCTX-M-3 Gene in Serratia marcescens Isolates (original) (raw)
Related papers
In Vivo Acquisition of a Plasmid-Mediated blaKPC-2 Gene among Clonal Isolates of Serratia marcescens
Journal of Clinical Microbiology, 2010
Three patients admitted to a Greek hospital were infected with Serratia marcescens isolates that exhibited reduced susceptibility to carbapenems and harbored Klebsiella pneumoniae carbapenemase (KPC) enzymes. In two of these cases, the patients were initially infected by carbapenem-susceptible S. marcescens isolates. Molecular typing and plasmid analysis suggested that all three patients had clonally indistinguishable isolates of S. marcescens that acquired a plasmid-mediated bla KPC-2 gene during the hospitalization.
FEMS Microbiology Letters, 2010
We report a Serratia marcescens and an Escherichia coli isolate simultaneously detected in the same patient. Both isolates showed susceptibility patterns suggestive of harbouring a plasmid-mediated AmpC beta-lactamase (pACBL) and a plasmid-encoded quinolone resistance (PMQR). PCR-based replicon, MOB typing, plasmid profile and Southern hybridization analyses revealed that both isolates coharboured bla(DHA-1) and qnrB genes on the same IncL/M-MOB(P13) plasmid approximately 70 kb in size. Together with the fact that both plasmids were conjugative in the laboratory, these results strongly suggest that a horizontal transfer event could take place in vivo. This is the first report of an isolate of S. marcescens harbouring a pACBL. The only phenotypic method that suggests the presence of a pACBL in an isolate harbouring an inducible chromosomal AmpC enzyme is the observation of scattered colonies near the edge of the inhibition zones of some beta-lactams. The presence of both resistance genes on the same plasmid and the reported increase in PMQR could perhaps explain the widespread distribution of bla(DHA-1) genes.
Replicon typing of 71 multiresistant Serratia marcescens strains
Research in Microbiology, 1994
Repticon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon Irep) groups which can often be correlated with incompatibility (lnc} groups. We studied 71 multiresistant Serratia marceseens strains with 19 rap probes constructed from reference plasmid replicons belonging to known tnc groups. These probes are known to react with enteric bacterial plasmids, However. they did not repcesent the totality of the thirty known Inc groups. For 52 % of the studied strains, plasmids were identified and classified into groups FIB, FIC, FIIA, HI2, L/M, N, B/O, P, W, Y and Comg. Most (79 %l of the plasmid preparations hybridized with a single rep probe, and 21% hybridized with two different probes. Electrophoretie analysis of DNA suggested that double hybridization could result from the presence of either two different Inc plasmids in the same strain (e.g. S37] or one single plasmid with a multireplicon (e.g. $113).
Infection and Drug Resistance, 2021
Gram-negative rods of the genus Serratia play an increasing role as etiological agents of healthcare-associated infections (HAI) in humans. These bacteria are characterized by natural and acquired resistance to several groups of antibacterial agents. The aim of the study was to characterize class 1, 2 and 3 integrons in the clinical isolates of Serratia spp. in Poland. Methods: The study comprised 112 clinical strains of Serratia, isolated from patients hospitalized in Poland in 2010-2012. Identification of strains was confirmed using MALDI-TOF MS (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry) system. Detection of class 1, 2 and 3 integrase DNA sequence was performed by multiplex-PCR. Amplicons obtained in the PCR reactions were purified and then sequenced bidirectionally. Results: Among the analyzed strains, Serratia marcescens was a predominant species (103/112, 92.0%). All three classes of integrase DNA sequence were detected in the analyzed strains of Serratia spp. DNA sequence of class 3 integron, besides integrase gene, revealed three gene cassettes (dfrB3, bla GES-7 ,bla OXA/aac(6ʹ)-Ib-cr). BLAST analysis of DNA sequence revealed that class 3 integron was carried on 9448 bp plasmid which was named pPCMI3-whole sequence of its DNA was submitted to GenBank NCBI (National Center for Biotechnology Information)-NCBI MH569711. Conclusion: In this study, we identified a new plasmid pPCMI3 harboring class 3 integron. This is the first report of a gene oxa/aac(6ʹ)-Ib-cr coding for a novel fusion protein, which consists of OXA β-lactamase and acetyltransferase aac(6ʹ)-Ib-cr. In the analyzed strains, class 1 and 2 integrons were also detected. Among the strains with class 1 integron, nine contained cassette array 5ʹCS-aadA2-ORF-dfrA12-3ʹCS, and two-cassette array 5ʹCS-aacC1-ORF-ORF-aadA1-3ʹCS, which were not previously reported in Serratia spp.
FEMS Microbiology Letters, 2021
Serratia marcescens SCH909 is a multidrug resistant strain isolated in 1988 harboring three class 1 integrons. We wondered if these integrons were retained over time and if there were other antimicrobial resistant determinants contributing to its multidrug resistant profile. Genomic analysis showed a fourth multidrug resistance integron, a Tn7 transposon with dfrA1-sat2-ybeA-ybfA-ybfB-ybgA gene cassettes in the variable region. Insertion sequences were involved in the genesis of novel composite transposons in the L4 subtype plasmid pSCH909, such as Tn6824 carrying an arsenic regulon and two head to head class 1 integrons surrounded by two complete IS1. Remarkably, a novel chromosomal genomic island, SmaR, was identified, closely related to Multiple Antimicrobial Resistance Regions (MARR), usually found in AbaR0-type and AbGRI2-0 from global clones of Acinetobacter baumannii, and in M-type plasmids circulating in Enterobacteriaceae. Maintenance studies showed that the three class 1 i...
Genetic analysis of the Serratia marcescens N28b O4 antigen gene cluster
Journal of bacteriology, 1999
The Serratia marcescens N28b wbbL gene has been shown to complement the rfb-50 mutation of Escherichia coli K-12 derivatives, and a wbbL mutant has been shown to be impaired in O4-antigen biosynthesis (X. Rubirés, F. Saigí, N. Piqué, N. Climent, S. Merino, S. Albertí, J. M. Tomás, and M. Regué, J. Bacteriol. 179:7581-7586, 1997). We analyzed a recombinant cosmid containing the wbbL gene by subcloning and determination of O-antigen production phenotype in E. coli DH5alpha by sodium dodecyl sulfate-polyacrylamide electrophoresis and Western blot experiments with S. marcescens O4 antiserum. The results obtained showed that a recombinant plasmid (pSUB6) containing about 10 kb of DNA insert was enough to induce O4-antigen biosynthesis. The same results were obtained when an E. coli K-12 strain with a deletion of the wb cluster was used, suggesting that the O4 wb cluster is located in pSUB6. No O4 antigen was produced when plasmid pSUB6 was introduced in a wecA mutant E. coli strain, sugg...
Cloning and DNA sequence analysis of a bacteriocin gene of Serratia marcescens
Journal of General Microbiology, 1992
Serratia marcescens N28b synthesized and secreted a bacteriocin, with a molecular mass of 45 kDa, which was capable of inhibiting the growth of Escherichia coli. The expression of this bacteriocin was negligible unless induced with mitomycin C. The genes encoding the bacteriocin were cloned in plasmid pBR328. E. coli harbouring recombinant plasmid pBA189 or pBA289 expressed the Serratia marcescens N28b bacteriocin. The nucleotide sequence of the bss gene (Serratia marcescens N28b bacteriocin structural gene) was determined. The predicted amino acid sequence of the carboxy-terminal part of the bacteriocin 28b had a high degree of similarity to the pore-forming domains of colicins A, E1, B, N, Ia and Ib.
Journal of Clinical Microbiology, 2010
Three patients admitted to a Greek hospital were infected with Serratia marcescens isolates that exhibited reduced susceptibility to carbapenems and harbored Klebsiella pneumoniae carbapenemase (KPC) enzymes. In two of these cases, the patients were initially infected by carbapenem-susceptible S. marcescens isolates. Molecular typing and plasmid analysis suggested that all three patients had clonally indistinguishable isolates of S. marcescens that acquired a plasmid-mediated bla KPC-2 gene during the hospitalization.
Bacteriocin 28b, a chromosomally encoded bacteriocin produced by most Serratia marcescens biotypes
Research in Microbiology, 1995
Twenty.dx ~atia marcescens strains belonging to fifteen different biotvpes were found to produce bactedccins ab-tive against E~ia coll. On the basis of the pattern of bacteriofin sensitivity of E. coil mutant strains, immunological assays and Southern blot hybridization with a probe for the S. marcescens bss (bacteriocin 28b ttmetural) gene, it was concluded that all these strains apperenth/prodeee chromosomalb/encoded baetedocins related to bectedocin 28b. To confirm thb conclusion, the genu encoding the bactldocin produced by one of these strains (S. marcesce~ JF246) were cloned in plasmkl pBR328. E. co//herboudng recombinant plmnnid pl)G301 prodluced a baetedocin aeti~ against E. co//and immunologically related to bacterioeio 2ab. Immunoblotti~ experiments showed that beetedocins 281) and L appear to have the same apparent molecular mass (45 kDa). Furthermore, recombinant plasmid pl)G301 DNA hybridized with a bss 9ene probe.
Antimicrobial Agents and Chemotherapy, 2002
We analyzed the role of integrons in the dissemination of antibiotic resistance in a recent multiresistant clinical isolate, Serratia marcescens SCH88050909 (SCH909). This isolate harbors three integrons, all on a 60-kb conjugative plasmid. By PCR, hybridization, and sequencing analyses, we found that integron 1 has the dfrA1 and ant ( 3 ") -Ia cassettes. The first cassette in integron 2 contains the ant ( 2 ") -Ia gene, separated from its attC site (59-base element) by a 1,971-bp insert containing a group II intron; this intron codes for a putative maturase-reverse transcriptase on the complementary strand and is the first such intron to be found associated with an integron. The attC site is followed by a novel aminoglycoside resistance gene, ant ( 3 ") -Ii-aac ( 6 ′) -IId , which has been characterized for its bifunctional ANT(3")-I and AAC(6′)-II activities. DNA sequence analysis of this fused cassette suggests that insertion and excision due to the integrase ...