Cytotoxic activity of methanolic fractions of different Marrubium spp. against melanoma cells is independent of antioxidant activity and total phenolic content (original) (raw)
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FEBS Open Bio, 2019
Running heading: Cytotoxic properties of Marrubium spp. extracts. Abbreviations FA-free phenolic acids, FB-phenolic acids after acidic hydrolysis, FC-phenolic acids after alkaline hydrolysis, DAD-diode array detector, TPC-total phenolic content, GAE-gallic acid equivalent, AA-antioxidant activity, DDPPH-2,2-diphenyl-1-picrylhydrazyl, MTT-3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, AAI-AA index
Evaluating Antiproliferative and Antioxidant Activity of Marrubium crassidens
Advanced pharmaceutical bulletin, 2014
Naturally occurring substances as novel drugs in cancer therapy, at all times, represent a challenge to science since medicinal plants are proving to be brilliant sources of new chemopreventive agents. In the present study, methanol extract from aerial parts of Marrubium crassidens was assessed for its antiproliferative activity in the breast cancer cell line MCF-7 through MTT bioassay using cell viability and cytotoxicity indices. The antioxidant property of M. crassidens extract together with its phenolic and flavonoids content were evaluated, as well. According to data obtained in the study, M. crassidens exhibited antiproliferative activity with a gradual rise in cytotoxicty effect setting out on 240µg/mL concentration of the extract. Moreover, the RC50 value for antioxidant activity of the extract was determined as 40µg/mL and values for the total phenolic and flavonoids were calculated as 512.64mg gallic acid equivalent and 212.73mg quercetin equivalent per 100g of dry plant m...
Acta Poloniae Pharmaceutica - Drug Research
The aim of the work done was the qualitative and quantitative assessment of a polyphenolic fraction contained in methanolic extracts obtained from the flowering herbs of Marrubium incanum Desr., M. peregrinum L., M. thessalum Boiss. & Heldr., and M. candidissimum L. The samples containing phenolic acids, having undergone acid and alkaline hydrolysis, were investigated by TLC, RP-HPLC, and Q-TOF-MS. In all methanolic extracts of Marrubium species, neochlorogenic, chlorogenic, protocatechuic, p-hydroxybenzoic, caffeic, p-coumaric, gentisic, ferulic acids and flavonoids: rutin, kaempferol rhamnohexoside, kaempferol hexoside, isorhamnetin hexoside, apigenin were identified. This is the first study dedicated to the qualitative and quantitative analysis of polyphenolic compounds in flowering herbs of selected Marrubium sp. Both the total phenolic content (TPC) and the antioxidant activity of water extracts obtained from aerial parts were assessed. TPC was estimated with the spectrophotometric method, whereas the antioxidant activity was evaluated by DPPH assay using Trolox, rutin, caffeic, and gallic acid as reference substances. We also presented the evaluation of antioxidant activity of 70% (v/v) methanolic extracts, that was expressed both as a percentage of DPPH radicals inhibition and IC 50 values (mg/mL). The same Marrubium extracts showed the highest concentration of phenolic acids. Ethanolic (70%, v/v), methanolic, acetone and water extracts of Marrubium sp. were screened for their antibacterial and antifungal activity using the agar microdilution method against a panel of reference strains of 20 microorganisms, including Gram-positive and Gram-negative bacteria and fungi belonging to yeasts.
DETERMINATION OF THE BIOACTIVE COMPOUNDS, ANTIOXIDANT AND ANTIFUNGAL ACTIVITIES OF DIFFERENT EXTRACTS OF Marrubium vulgare L, 2021
The current work concerns a medicinal plant Marrubium vulgare L. grown in Souk Ahras region (Northeastern of Algeria). Widely used by the natives of this region for their extraordinary therapeutic properties such as: antioxidant, antiinflammatory, antibacterial and analgesic activities. The aims of this study were to evaluate the phenolics, flavonoids and tannins composition, and antioxidant activities of hexane, dichloromethane, acetone and methanol extracts from leaves of Marrubium vulgare L. by using different methods. The extracts were a good source of phenolics and flavonoids. Acetonic extract from the aerial part of M. vulgare had the highest total phenolic and flavonoid contents with 171.45±3.38 µg GAE mg-1 E and 78.33±0.29 µg QE mg-1 E, respectively. While the hexane extract had the most condensed tannins content with 90±0.01 µg CE mg-1 E. The antioxidant activity showed the good antioxidant capacity of extracts (dichloromethane, acetone and methanol) in DPPH, ABTS, β-Carotene, CUPRAC, Phenanthroline and GOR assays. While the hexane extract showed the lowest antioxidant activity in DPPH, β-Carotene and GOR assay. Weak antioxidant activity in Reducing power was observed for all extracts with >200 µg/mL. On the other hand, these extracts showed no activity in Ferrous ions chelating assay, exceptionally the methanol extract which showed a very weak activity >800 µg/mL. The results showed that the concentrations of TPC, TFC and CTC were not significantly different (p > 0.05). However, a highly significant difference in all antioxidant activities was observed (p < 0.001) except Phenanthroline activity which presented a non-significant difference (p > 0.05). We have also tested the effect of the different extracts against two phytopathogenic fungi strains by the disc-diffusion method. The inhibitory effect were important for the two tested strains. The extracts of M. vulgare have important antifungal activity. Statistical analysis indicates no significant differences at P > 0.05. Based on These results, it is right to conclude that M. vulgare is an important source of bioactive compounds with antioxidant and antifungal properties.
2014
In vitro antioxidant activity, total phenolic content and concentration of flavonoids of Marrubium vulgare methanolic extract were determined using spectrophotometric methods. The antibacterial activity was also evaluated against seven pathogenic bacteria using the disc-diffusion assay and broth dilution method. The obtained results showed that the tested extract was rich in phenol (26.8±0.01 mg Gallic acid per gram) than in flavonoids (0.61 ±0.05 mg Catechin equivalents per ml). The condensed tannin content was 0.086±0.01 mg ER/mL. In addition, the methanolic extract showed a strong antioxidant power against the DPPH (IC 50 = 35±0.01) and ABTS (IC 50 = 25±0.2) radicals scavenging. The tested extract had significant activity against both Gram-positive (B. cereus, L. monocytogens, S. aureus) and Gram-negative bacteria (E. coli, P. aeruginosa, A. hydrophila, S. typhimurium). The inhibition zones diameters and minimal inhibitory concentration values were in the range of 13-17 mm and 12.5-25 mg/ml, respectively. The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant and antibacterial activities. The M. vulgare methanolic extract can be regarded as promising candidate for natural plant source of antioxidant and antimicrobial.
Anti-Hepatotoxic Activity of Marrubium vulgare L. in Carbon Tetrachloride Induced Toxicity
Journal of Pharmaceutical Research, 2009
Different extracts of whole plant of Marrubium vulgare L (Lamiaceae) were investigated for hepatoprotective activity against carbon tetrachloride induced hepatic damage in male Albino Wister rats. The activity of these extracts (500-mg/kg b. w for 7 days) was compared with the standard drug silymarin (Silybon-70, 10 mg/kg-b.w). The petroleum ether, chloroform and methanolic extracts have shown significant hepatoprotective activity by reducing the elevated levels of serum enzymes such as serum glutamate oxaloacetate transaminase (SGOT) by 17.69, 67.0 and 30.80%, serum glutamate pyruvate transaminase (SGPT) by 19.0, 22.30 and 39.29%, alkaline phosphatase (ALP) by 17.32, 28.99, 35.38% and increased total protein (TP) by 22.49, 31.84, and 34.29% respectively indicating that the methanol extract was the most active among the three extracts as compared to standard drug silymarin, which decreased SGOT by 53.04, SGPT by 55.96, ALKP by 35.87 and increased TP levels by 59.24% against CCl 4 intoxicated rats in comparison to normal control. These biochemical observations were also supplemented by histopathological examinations of the liver sections. The results showed that the methanolic extract was the most active among all the extracts.
PSM Biological Research, 2021
The objective of the present study was to determine the pharmacological properties of the methanolic (MeOH) extract of Marrubium deserti leaves. For this purpose, antioxidant activity was carried out by DPPH and Ferric reducing power (FRAP) assays respectively while In vivo anti-inflammatory activity was tested by carrageenan-induced paw edema model. The Phytochemical investigation revealed the presence of several biocompounds, and total phenolic and flavonoid contents were also determined to support our results and revealed a high proportions of polyphenols (184 ± 0.78 mg GAE/g extract) and flavonoids (28.48 ± 0.40 mg QE/g extract). The MeOH extract demonstrated great pharmacological properties with a dose-effect relationship. Thus, a great antioxidant effect was recorded in both DPPH and FRAP assays with a respective IC50 of (15.1 μg/ml) and (80.01 ± 1 μg EAA/g of extract) and were considered significant (P<0.05) when compared to respective standards. On the other hand, anti-inflammatory results suggested that the plant extract could effectively oppose the inflammation caused by carrageenan at the dose of 200 mg/kg with significant decrease (84.1 %) of inflammation. These encouraging results suggest that our plant could be a good candidate to treat more effectively pathologies related to oxidative stress and inflammation.
Antiproliferative activity of Marrubium persicum extract in the MCF-7 human breast cancer cell line
Asian Pacific journal of cancer prevention : APJCP, 2012
Developing antitumor drugs from natural products is receiving increasing interest worldwide due to limitations and side effects of therapy strategies for the second leading cause of disease related mortality, cancer. The antiproliferative activity of a methanolic extract from the aerial parts of Marrubium persicum extract was assessed with the MCF-7 breast cancer cell line using the MTT test for cell viability and cytotoxicity indices. In addition, antioxidant properties of the extract were evaluated by measuring its ability to scavenge free DPPH radicals. Moreover, the total phenolic and flavonoid content of the extract was determined based on Folin-Ciocalteu and colorimetric aluminum chloride methods. The findings of the study for the antiproliferative activity of the methanolic extract of M. persicum showed that growth of MCF-7 cells was inhibited by the extract in a dose and time dependent manner, where a gradual increase of cytotoxicity effect has been achieved setting out on 2...
Marrubium vulgare L.: A Phytochemical and Pharmacological Overview
Molecules
Marrubium vulgare is a plant with high bioactive potential. It contains marrubiin, a labdane diterpene that is characteristic for this genus, as well as a complex mixture of phenolic compounds. According to numerous studies, M. vulgare acts as a good antioxidant agent, and due to this, it could potentially be useful in treatments of cancer, diabetes mellitus, and liver diseases. In addition, its anti-inflammatory, wound-healing, antihypertensive, hypolipidemic, and sedative potential are discussed. Apart from that, its antimicrobial activity, especially against Gram+ bacteria, fungi, herpes simplex virus, and parasites such as Toxoplasma gondii, Trichomonas vaginalis, and Plasmodium berghei-berghei was recorded. Additionally, it could be used as a chicken lice repellent, herbicide, and natural insecticide against mosquito larvae and natural molluscicide. In veterinary medicine, M. vulgare can be used as an anthelmintic against the eggs and larvae of bovine strongyles parasites, and ...