Seroprevalence of Neospora caninum in dairy cows in Belgrade city area, Serbia (original) (raw)
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AgroLife Scientific Journal, 2023
Neospora caninum is an apicomplexan protozoa parasite that causes reproductive disorders in cattle worldwide. Despite the high seroprevalence reported in Romania, there are limited data on N. caninum infection in dairy cattle, and no studies have evaluated the presence of infection over time in a limited area. Therefore, this study aimed to assess the seroprevalence of N. caninum in a dairy cattle farm in Southeastern Romania where a previously study reported about the cattle exposure to N. caninum infection. A total of 85 cattle, assigned in four groups, according to the reproductive status (repeated breeding syndrome, history of abortion, pregnant cows, and fresh cows) were tested for the presence of N. caninum antibodies, using an indirect ELISA test. The overall seroprevalence was 28.23% (95% CI: 18.67-37.80). According to the reproductive groups, the seroprevalence varied between 19.04% (95% CI: 2.25-35.84) in fresh cows to 38.09% (95% CI: 17.32-58.87) in cows with repeated breeding syndrome. By these findings new information is provided about the disease's presence in the studied farm, implying that infection with N. caninum may be the primary cause of reported reproductive disorders, as well as about the infection's persistent nature.
Serological survey of Neospora caninum infection in cattle herds from Western Romania
The Journal of parasitology, 2012
Serum samples from 376 randomly selected adult cattle, from 25 farms located in 3 counties (Arad, Bihor, and Timiş) from western Romania, were sampled for Neospora caninum antibodies using a commercial ELISA-kit. Seroprevalence values and risk factors for neosporosis (cow age, breed, herd size, farming system, previous abortion, and number of farm dogs) were examined using a generalized linear mixed model with a binomial distribution. Overall, the seroprevalence of N. caninum was 27.7% (104/376) with a prevalence of 27.9% (24/86) in Arad, 26.9% (25/93) in Bihor, and 27.9% (55/197) in Timiş. Of 25 cattle herds, 23 were seropositive with a prevalence ranging from 10.0 to 52.2%. No correlation was found between N. caninum seropositivity and age, breed, herd size, breeding system, and previous abortion. The number of farm dogs was the only factor (P(Wald) = 0.03) positively associated with seroprevalence in cows and can be considered the risk factor in the acquiring of infection. The ...
The incidence of Neospora caninum in dairy cows with abortion and infertility problems
Kocatepe veteriner dergisi, 2021
The contribution of animal husbandry to the national economy cannot be ignored. The causes of infertility are various and complex. Economic losses are increasing in dairy cattle breeding due to infectious and non-infectious causes. The percentage of abortion due to infectious agents is not fully known, but infectious agents lie in about 90% of the cases whose etiology can be determined. Neospora caninum is considered to be one of the most important abortion factors of cattle. Although, this protozoa is always ignored. It causes abortion in cows. In this study, it was aimed to detect N. caninum from the blood serum obtained from 137 dairy cattle brought to Aksaray University Faculty of Veterinary Medicine between 2017-2019 and suffering from abortion and infertility problems. For serological diagnosis, ELISA (Enzyme-Linked ImmunoSorbent Assay) test was performed. As a result, N. caninum agent was detected in 37.22 % (51/137) of the samples that belong to cattle with abortion and infertility problems. It was concluded that economic loss due to infertility in dairy cattle and protozoa, which are one of the infection factors, should be given importance.
Neospora caninum infection dynamics in dairy cattle
Parasitology Research, 2012
Neospora caninum is considered in many countries as one of the key abortion agents in cattle. This study aims to investigate the parasite behavior in dairy cattle in the municipality of Avaré, SP, Brazil, where abortions frequently occur. An ELISA was performed to compare two samplings on a total of 615 animals; tests were performed in the same herds with a gap of 3 years. An increase in the percentage of reactive animals was observed, ranging from 21.6% at the first sampling to 38.9% at the second sampling. Of the 176 animals tested at both samplings, 61.93% retained a non-reactive status, 15.9% retained a reactive status, 19.88% switched from non-reactive to reactive and 2.27% switched from reactive to non-reactive. Of the 100 animals with reproductive disturbances, 50% presented anti-Neospora antibodies, thereby indicating the presence of the protozoa. When comparing cows and their respective female offspring, a predominance of horizontal infection was observed. Moreover, considering the significant percentage of animals that switched from non-reactive to reactive and the abundant presence of dogs among the herds, the N. caninum transmission may be attributed to presence of carnivores.
Theriogenology, 2004
This study was carried out to determine the prevalence of neosporosis in an area of intensive dairy production, in Portugal. Sera samples were obtained in a random basis from 114 cows in 49 herds (group A), and from 1237 cows in 36 herds with a history of abortion outbreaks (group B). All sera samples were tested for neosporosis by direct agglutination test (DAT). Additionally, attempts to isolate Neospora caninum in 42 aborted bovine fetuses from 38 dairy herds (group C) were carried out, utilizing a bioassay with immuno-depressed Swiss Webster mice. Parasitological confirmation was done by indirect fluorescent antibody test (IFAT). The prevalence of neosporosis in the group A was 28%. Group B had a significantly (P < 0:001) higher prevalence (46%) and Neospora caninum was isolated in 36% of the aborted fetuses (group C). These results indicate that neosporosis, a disease only recently (2001) diagnosed in Portugal, has a high prevalence in the country, particularly in populations with a story of abortion. Thus, neosporosis should systematically be considered in the differential diagnosis of abortion. In the context of embryo transfers, the importance of selecting Neospora-free embryo recipients is discussed, as well as the pertinence of assessing the Neospora status of traded and imported cattle.
Seroprevalence of Neospora Caninum in Dairy Cattle Herds in Central Anatolia,Turkey
Veterinarski Arhiv, 2006
A total of 3287 sera collected from cows in thirty-two herds in the eight provinces of Central Anatolia of Turkey were tested for presence of Neospora caninum antibodies by using commercial ELISA kit. The overall seroprevalence of Neospora caninum was 13.96% (95% CI 13.78 to 16.22). On a provincial basis, N. caninum infection was present in all provinces while cows sampled in Kirikkale had the highest rate (P<0.05). Antibodies to N. caninum were found in 34 (23.61%) of 144 aborted cows. The prevalence of N. caninum was significantly higher in the aborted cows than in non-aborted cows (P<0.001). However, the odds ratio was 1.97, suggesting an association between abortion and seropositivity. This is the first report on serological prevalence of Neospora caninum in cows in Central Anatolia, and neosporosis in dairy cattle seems to be widespread in some provinces of this region.
Study on outbreak of Neospora caninum-associated abortion in dairy cows in Calabria (Southern Italy)
Large Animal Review, 2015
Introduction Neospora caninum is an apicomplexan protozoon causing abortion in cattle worldwide. Infected cows represent economic losses due to fetal abortion, stillbirth, clinical and subclinical disease, impaired milk production, neonatal deaths, reduced fertility and reduced value of infected cows. The abortion caused by neosporosis occurs at 5-6 months gestation. Serological techniques are primarily employed to detect specific antibodies against N. caninum to differentiate infected from non-infected animals and to assess the situation into a herd with regard to abortions due to infection with N. caninum. The herd prevalence rates of N. caninum infection reported in several European countries range from 16% in Sweden to 80% in Spain. Aim The present study was designed to assess the role of N. caninum for causing abortion in dairy farms in the central area of Calabria region (Southern Italy), by applying the ELISA serologic test. Materials and methods For the study of outbreak of ...
The purpose of this study is to determine the seroprevalence of N. caninum in cows with abortion story and in dogs (n = 40) living in the same area using a competitive enzyme-linked immunosorbent assay (cELISA). Among the 11 villages regrouping 78 herds (2082 cows) explored in the Kars region, the total abortion rate was 7.1% (148 cows) and among them 7.4% (11 cows) were seropositive for N. caninum and belonged to 7 villages. The neosporosis seroprevalence was similar for local breeds (7.6%) and for Simmental breed (6.3%) and was slightly higher but not significantly in young females (1.5-3 years old). In the great majority of seropositive cases (81.8%), cows have aborted between the 6 th and the 8.5 th gestational months, corresponding to the winter months. In dogs, seropositivity rate was 12.5% and positive dogs, mainly 1.5-3 years old, stemmed from 2 villages in which seropositive cows were detected. In conclusion, the association of N. caninum in cows and in dogs sharing the same environment in the Kars region was observed, but transmission modalities require further investigations.
A long-term study of Neospora caninum infection in a Swedish dairy herd
Acta veterinaria Scandinavica, 2003
A longitudinal study was performed in a Swedish dairy herd where Neospora caninum had been isolated from a stillborn calf. Starting in autumn 1994, blood samples from all female animals in the herd were collected once yearly until 1999. The sera were analysed for presence of IgG1 antibodies to N. caninum by the iscom ELISA, and by an avidity ELISA to establish the timing of infection. In addition, data on reproductive performance were compiled. During the study the percentage of seropositive female animals increased from 63% to 87%. In 1994 a large number of young animals tested seropositive although their dams were seronegative, indicating that a transmission of the parasite other than the vertical had recently occurred. Low avidity values supported this assumption. The annual abortion rate increased from a mean of 2% before the initiation of the study to 9% in 1994-1998. During the same time, as judged by the avidity data, a large proportion of the animals shifted from being recen...