Comparison of the Antibacterial Efficacy ofCommiphora molmoland Sodium Hypochlorite as Root Canal Irrigants againstEnterococcus faecalisandFusobacterium nucleatum (original) (raw)
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Root Canal Irrigation: Chemical Agents and Plant Extracts Against Enterococcus faecalis
The Open Dentistry Journal, 2016
Background:There are various microorganisms related to intra and extra-radicular infections and many of these are involved in persistent infections. Bacterial elimination from the root canal is achieved by means of the mechanical action of instruments and irrigation as well as the antibacterial effects of the irrigating solutions.Enterococcus faecaliscan frequently be isolated from root canals in cases of failed root canal treatments. Antimicrobial agents have often been developed and optimized for their activity against endodontic bacteria. An ideal root canal irrigant should be biocompatible, because of its close contact with the periodontal tissues during endodontic treatment. Sodium hypoclorite (NaOCl) is one of the most widely recommended and used endodontic irrigants but it is highly toxic to periapical tissues.Objectives:To analyze the literature on the chemotherapeutic agent and plant extracts studied as root canal irrigants. In particularly, the study is focused on their ef...
Objective : the study was designed to compare the antimicrobial efficacy of 5% Sodium hypochlorite solution and 10% Neem leaves extract against infected root canal microbial isolates with special reference to Enterococcus Faecalis and Candida Albicans using Agar diffusion method. Materials and Method: A total of 50 patients requiring endodontic therapy with chronic periapical infection were selected and samples were isolated under strict aseptic protocol from the root canal systems. The samples were subjected to check the antimicrobial efficacy of Group A – 5% Sodium hypochlorite solution and Group B – 10% Neem leaves extract and Group C – distilled water as a control group. Agar well diffusion method was used for incubation and zones of inhibition was recorded. Results were subjected to ANOVA test and other statistical analysis using SPSS software. Results : It appeared that Group A and Group B are significantly effective in antimicrobial efficacy against E. Faecalis and C. albicans except Group C where no zones of inhibition was seen. The difference between scores obtained with Sodium hypochlorite solution and Neem leaves extract was statistically significant (P=0.05) Conclusion : 5% Sodium hypochlorite solution and Neem leaves extract has significant antimicrobial effect against E. faecalis and C. albicans with neem being possessing a significant property of biocompatibility to oral and periapical tissues Keywords: Phytodentistry, Azadirachta Indica, Ramifications, Immunomodulatory, Brain Heart Infusion Agar
2010
Purpose: This study assessed the antimicrobial effectiveness of an iodoform-based paste (Guedes-Pinto Paste, GPP) and a paste modified by the addition of a 2% chlorhexidine gluconate gel (CHX) to replace the camphorated parachlorophenol component of the original GPP. Methods: The antimicrobial action was tested against the following microorganisms: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus oralis, Enterococcus faecalis, Escherichia coli and Bacillus subtilis. In the agar diffusion test, 18 Petri plates with 20 mL of BHIA were inoculated with 0.1 mL of the microbial suspensions. Paper disks were immersed in the experimental solutions for 1 min and were then placed over the BHIA surface in each agar plate. The plates were maintained for 1 h at room temperature and then incubated at 37ºC for 48 h. The diameters of the microbial inhibition halos were measured around the paper disks containing the substances. Data were analyzed using the Mann-Whitney U-test (α=0.05...
Journal of Pharmaceutical Research International
Nigella sativa (kalonji) has been used since ancient times as a nutritional supplement and for treating various infections and chronic ailments. As pathogens become resistant to most drugs, kalonji can be used as an alternative compound in modern medicines. The use of herbal extracts as endodontic irrigants might be beneficial as a part of a growing trend to seek natural remedies for dental treatment. Aim: To compare the antibacterial potency of Aqueous Nigella sativa extract, Aqueous Neem leaf extract, and 3% Sodium Hypochlorite. Materials and Methods: Test solutions were tested against E. faecalis (ATCC 29212) to check for their Minimum Inhibitory Concentration (MIC) by double dilution method and Kill time to measure their antimicrobial potency to be used as an intracanal irrigant. Results: The study's limitations show that Aqueous Nigella sativa extract has a better antimicrobial effect than Aqueous Neem leaf extract and 3% Sodium Hypochlorite solution against E. faecalis.
Journal of …, 2007
Introduction: Bacteria and their products play a primary etiological role in the initiation and perpetuation of pulpoperiapical pathosis. Intracanal medication is important for endodontic success as it eliminates microorganisms that persist after chemomechanical preparation. Aim: To compare antimicrobial efficacy of calcium hydroxide powder, triple antibiotic paste, calcium hydroxide with 2% chlorhexidine solution, and triple antibiotic paste with 2% chlorhexidine solution. Materials and methods: A total of 48 nonvital primary teeth were included in this study. After access opening first microbiological sample (s1) was collected by using absorbent paper point introducing into canal. Second microbilogical sample (s2) was taken following chemomechanical preparation and the teeth were divided into four groups: Group I: calcium hydroxide (CH) powder with distilled water; group II: CH with 2% chlorhexidine solution; group III: triple antibiotic powder with distilled water; group IV: triple antibiotic paste with 2% chlorhexidine solution. Then the canals were filled with any one group of the medicament and cavity was temporarily sealed with zinc oxide eugenol. After 1 week, a postmedication sample (s3) was collected. Then the canal was filled with Metapex, restored with glass ionomer cement. Conclusion: From the experiments carried out in this study, with the limitations, an inference can be drawn that a combination of antimicrobial agent used as intracanal medicament is definitely better than single agent like Ca(OH) 2 .
The Journal of Contemporary Dental Practice, 2013
Aim This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a final rinse against Enterococcus faecalis. Materials and methods Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours. Results Statistical analysis showed that there was a significant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation Conclusion The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. Clinical significance E. faecalis is one of the most resistant intracanal species an...
2010
Aim: The aim of this study was to assess the antibacterial activity of seven different root canal sealers used in primary teeth against Enterococcus faecalis. Material and Method: The study was conducted using the agar diffusion inhibitory test. The sealers used were Sure-paste, Metapex, Tg Pex, Calcicure, Endoflas FS, Sealapex, Ca(OH)2 mixed with %2 Chlorhexidine Gluconate solution and one control group material (Klorhex Oral Gel). The injectable form sealers and the freshly-mixed sealers were placed into the prepared wells on the agar plates which were inoculated with the Enterococcus faecalis. After incubation periods of 24 hours, 48 hours, and 72 hours, the zones of the growth inhibition were observed and measured. The average of 8 measurements for each material was taken. The data were compared using one-way analysis of variance (ANOVA) (p<0.05). Results: Even though Sure-paste, Calcicure and Ca(OH)2 powder mixed with Klorhex irrigation solution have shown antibacterial acti...
Jaypee's international journal of clinical pediatric dentistry, 2024
Dental caries, an infectious process that involves the breakdown of tooth enamel, is a multifactorial disease caused by a complex interaction between cariogenic acid-producing bacteria in combination with fermentable carbohydrates and other dietary, genetic, behavioral, social, and cultural factors. 1-3 Dental caries is a progressive and polymicrobial disease involving enamel, dentin, and eventually the vascular tissue pulp. The inflammation of the pulp involves endodontic intervention as a treatment, which aims at decreasing and eliminating microbial flora with chemical and mechanical measures in single or multiple visits. Root canal flora consists of obligatory anaerobes, particularly black-pigmented gram-negatives, which produce signs and symptoms; aerobes and facultative anaerobes, such as Enterococcus, Candida, and alpha Streptococcus, though in low proportion, are considered
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2009
The purpose of this study was to determine the antimicrobial efficacy of sodium hypochlorite adjusted to pH 12, 7.5, and 6.5 in human root canals infected by Enterococcus faecalis. One hundred sixty-five human single-rooted teeth were prepared and inoculated with E. faecalis for 48 h. Teeth were divided into 3 experimental groups according to the irrigation pattern used: group 1, 4.2% NaOCl pH 12; group 2, 4.2% NaOCl pH 7.5; and group 3, 4.2% NaOCl pH 6.5. Samples from the root canals were collected, and bacterial growth was analyzed by turbidity of the culture medium. None of the irrigating solutions used in this study demonstrated 100% effectiveness against E. faecalis. The antibacterial effectiveness of 4.2% NaOCl at pH 6.5 was significantly increased (P = .03) compared with 4.2% NaOCl at pH 12 (chi-squared test: P &amp;amp;amp;amp;amp;lt; .05). Bactericidal activity of NaOCl solution is enhanced by weak acidification of 4.2% NaOCl solution at pH 6.5.