Evaluation of Candida Albicans Attachment with Two Types of Denture Base (Heat Cured Acrylic Flexible Resin) Polished By Different Polishing Materials (original) (raw)
Related papers
Journal of clinical and diagnostic research : JCDR, 2013
Candidiasis is common in denture wearers. Rough surfaces of dentures promote denture plaque formation and maturation, thus justifying the need for smooth surfaces. Proper finishing and polishing of dentures provide smooth surfaces. Thus, this study was done to assess the adherence of Candida to heat polymerized acrylic resin surfaces which were subjected to different stipulated polishing methods. A total of 48 acrylic resin specimens of dimension 10x10x3 mm(3) were fabricated and divided into four groups, A, B,C and D, with 12 specimens in each group, according to method of polishing step which was employed. Subsequently, they were subjected to an adherence assay. All the data was statistically analyzed by using One-way ANOVA and differences within the groups were analyzed by using independent sample t-test. RESULTS revealed that group A which was unpolished had a mean adherence of 2.92 and it was finished by using sand papers. Group B had a mean adherence of 0.5 and it was finished...
Background: The adherence of Candida albicans to host cells or polymers such as denture acrylic resin is an essential and necessary first step in successful colonization and the development of pathogenesis and infection. A denture may then function as a reservoir of infection and surface irregularities can increase the adherence of microorganisms even after adequate hygiene measures. Aim and Objectives: To compare and evaluate the adhesion of Candida albicans on heat polymerized polymethyl methacrylate (PMMA) denture base resin with three different surface finishes. Materials and method: To evaluate and compare adherence of Candida albicans on three different surface finishes of PMMA. Two commercial brands namely, Trevalon (Dentsply; Gurgaon, India), DPI (Dental Products of India; Mumbai, India) and a reference strain of Candida albicans (ATCC 60193) was used for this experimental purpose. Viable and adherence assay methods were used to evaluate this adherence. Results: The results of this study confirm earlier work which demonstrated that increased surface roughness increased retention of yeast on PMMA surfaces. The results obtained by adherence assay revealed results similar to the viable assay with higher adherence of Candida albicans to rough surface as compared to the polished surface. Conclusion: Surface finished with tungsten carbide bur had the maximum roughness and adherence of Candida albicans to its surface was the highest. DPI acrylic denture base material had less number of adherent Candida albicans cells to its surface with similar finish than that of Trevalon.
Biomedical and Pharmacology Journal, 2018
The aim of this study was to evaluate the effect of modified heat cured polymethyl methacrylate denture base (PMMA) or varnish coating of the denture base on candida albicans adhesion and porosity. First experimental group was prepared by addition of 15% phosphoric acid 2-hydroxyethyl methacrylate ester with polymethyl methacrylate monomer. While second experimental group prepared by varnish layer coating after material processing, these groups were compared with the control one. Three groups of vertex acrylic resin used as control, mPMMA and varnish coated group (PPH Cerkamed Wojciech Pawlowski, Poland). The inverted light microscope was used to measure the candida albicans adherence after one week and one month of processing. Two-way ANOVA, LSD test, T- test and Dunnett T3 were performed on data. Modified Acrylic resin showed highly significant reduction in candida albicans adhesion and porosity, also for varnish coating group showed highly significant reduction in candida albican...
This study evaluated the surface roughness and Candida albicans adherence on denture base acrylic resins and silicone-based resilient liners with different surface finishes. Four commercial denture base acrylic resins (three heat polymerized and one room temperature polymerized) and five silicone-based liner materials (two heat polymerized and three room temperature polymerized) (10×10×2 mm) were tested in this study. The materials were processed against glass or plaster or finished with a tungsten carbide bur. Surface roughness measurements were made using a profilometer with an optical scanner probe. All specimens were ultrasonically cleaned in water for 15 s, autoclave sterilized, and contaminated with C. albicans solution for adherence assay evaluation. The materials processed against the glass surface showed significantly lower surface roughness values (0.11±0.1-1.66±1.1 μm) than those of the materials processed against the dental plaster (2.61±0.2-6.12±2.8 μm) or roughening with a bur (1.48±0.2-7.05± 1.2 μm; p<0.05, one-or two-way analysis of variance). Also, the materials processed against the glass surface showed lower C. albicans adhesion (mean ranks 120.36) than those of the materials processed against the dental plaster (mean ranks 139.77) or roughening with a bur (mean ranks 143.06), but the differences were not statistically significant (p>0.05, Kruskal-Wallis and Mann-Whitney). In all types of surface finishes, C. albicans adhesion on denture base acrylics was significantly less (mean ranks 90.18-90.40) than those of silicone liners (mean ranks 119.38-205.18; p<0.01, Kruskal-Wallis).
Journal of Materials Science: Materials in Medicine, 2018
The aim of this study is to evaluate the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer coating with various concentrations onto acrylic resin denture base material on surface characteristics such as contact angle and surface roughness and on Candida albicans adhesion which is the major factor of denture stomatitis. Specimens, prepared from heatpolymerized acrylic denture base material, were divided into control and three test groups, randomly. Surfaces of the specimens in test groups were coated with poly(MPC) (PMPC) by graft polymerization of MPC in different concentrations (0.25 mol/L; 0.50 mol/L and 0.75 mol/L), while no surface treatment was applied to the control group. Contact angles and surface roughness were examined, and chemical composition of the surfaces was analyzed by Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (FTIR) to verify the presence of PMPC coatings. Then, specimens were incubated with C. albicans for 18 h and the number of adhered cells was determined. Upon PMPC coating, the contact angle values statistically decreased, but no difference was found in surface roughness values. A statistically significant decrease was observed in C. albicans adhesion in parallel with the increase in the MPC polymer concentration. There was no significant difference between 0.50 mol/L and 0.75 mol/L groups in terms of adhesion. These findings indicated that graft polymerization of MPC on acrylic denture base material reduces the adhesion of C. albicans, and may be evaluated as a coating for prevention of denture stomatitis.
Journal of dentistry (Shiraz, Iran), 2017
Candida species are believed to play an important role in initiation and progression of denture stomatitis. The type of the denture material also influences the adhesion of candida and development of stomatitis. The aim of this study was comparing the adherence of candida albicans to the conventional and injection molding acrylic denture base materials. Twenty injection molding and 20 conventional pressure pack acrylic discs (10×10×2 mm) were prepared according to their manufacturer's instructions. Immediately before the study, samples were placed in sterile water for 3 days to remove residual monomers. The samples were then sterilized using an ultraviolet light unit for 10 minutes. 1×10(8) Cfu/ml suspension of candida albicans ATCC-10231 was prepared from 48 h cultured organism on sabouraud dextrose agar plates incubated at 37oC. 100 μL of this suspension was placed on the surface of each disk. After being incubated at 37oC for 1 hour, the samples were washed with normal saline...
Texila International Journal of Public Health, 2024
Acrylic resin is the most common material used for the fabrication of bases for prostheses including overdentures. A smooth surface is ideal for the maintenance of dentures and the prevention of bacterial colonization. Polishing materials used and the method of moulding are the few factors that affect surface roughness. Hence the present in vitro study was done to compare the effect of polishing materials and moulding methods on the surface roughness of dentures. 120 specimens (50mm × 3mm) were divided into two equal groups and fabricated by compression and injection moulding techniques. Each group was subdivided into 3 groups based on the polishing method viz: control (pumice), pumice with universal polishing paste and pumice with rouge based on the polishing protocols advocated. Determination of surface roughness was done with a profilometer. Statistical analysis was done with one-way ANOVA and Tukey HSD. The surface roughness ranged between 0.372 ± 1.340 and 0.046 ± 0.027. In both the moulding methods control group with pumice exhibited the greatest surface roughness in comparison with the other two groups and universal polishing paste produced the least. On comparing the two moulding methods the injection moulding method exhibited the least surface roughness. (p=0.0001). From the results of the study, it can be inferred that irrespective of the moulding method polishing using pumice and universal polishing paste would produce a smooth surface with the least roughness and could be implemented by all clinicians to bacterial colonisation and plaque formation in dentures.
Comparison of Candidal and Bacterial Adherence to Denture Base Acrylic Resins
2013
Background and Aim: Futura Gen and GC Reline Hard denture chairside reline material shave recently been introduced to the dental market. Although yet to be scientifically proven, these products are claimed to have optimal characteristics. This study aimed to compare the adherence of Candida strains and bacteria to denture base acrylic resins. Materials and Methods: In this in-vitro experimental study, 24 discs were fabricated of Futura Gen, GC Reline Hard and Meliodent reline acrylic resins. Specimens were inoculatedwith three Candida strains and Streptococcus mutans at three time points and after staining,their optical absorbance (microbial adhesion and biofilm formation) was calculated using spectrophotometry. Two-way ANOVA and Tukey’s HSD test were applied for statistical analysis. Results: Absorbance (optical density)of C. albicans strains on Futura Gen, GC Reline and Meliodent hard reline acrylic resins was 0.022, 0.011 and 0.028 at one hour, 0.057, 0.022 and 0.062 at 24 hours ...
Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro
Materials
Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease i...
2012
background and Aim: This study aims to investigate adhesion of Candida albicans and flexural strength of an acrylic resin repaired with 4 different acrylic materials. materials and methods: One hundred heat polymerizing acrylic specimens (60x20x3mm) were prepared from a negative mold of a metal master model. After obtaining silicone indexes the specimens were cut into two equal parts with a saw (Isomet 4000, Buehler Ltd., Lake Bluff, IL). Four different materials light-polymerizing (LP), autopolymerizing (AP), heat polymerizing (HP) or microwave polymerizing (MP) acrylic resins were used to repair the denture material. Fifty of the specimens were then contaminated with Candida albicans culture. Three point bending test was used to determine flexural strengths of materials. The data were analyzed with One-way ANOVA and Tukey statistical tests at 0.05 significance level. r esults: Light polymerizing acrylic resin represented the highest colony number values (49.3±6.2) and this value w...