Preliminary Bioactive Compounds Screening and Antibacterial Activity of Methanolic Extract of Hibiscus rosasinensis against Selected Skin Pathogens. (original) (raw)
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2015
Hibiscus rosa sinensis is member of the family Malvaceae and grows as an evergreen herbaceous plant in tropical regions. Hibiscus species are used in the treatment of various diseases. They have many pharmacological properties including antipyretic, antispasmodic, hypotensive, antifungal, anti-inflammatory and many more. Therefore, the present study was aimed at evaluating the antibacterial activity of methanolic extract from the leaves and flowers of H. rosa sinesis and identification of secondary metabolites in these extracts. The phytochemical analyses showed the presence of alkaloids, glycosides, flavonoids, tannin and phenols in hibiscus leaf extract, while hibiscus flower extract contained alkaloids, protein, steroid and carbohydrate. Antibacterial activity has been checked in terms of zone of inhibition by disc diffusion method against microorganisms E.coli and S. aureus for different concentrations of methanolic leaf and flower extracts ranging from 31.25 to 500 mg/disc whic...
Studies on biological activities and phytochemicals composition of Hibiscus species-A review
This article reviews the antimicrobial and antioxidant activities as well as the phytochemical composition of extracts from some Hibiscus species. Some of the bioactive constituents of these plants were isolated, purified and analyses for possible use in making drugs. Thus these plants have great medicinal potential for the therapy of infection. [Mohamed Z.M. Salem, AZM Salem. Studies on biological activities and phytochemicals composition of Hibiscus species-A review.
The Pharma Innovation
Infectious diseases are the second leading cause of death worldwide, killing almost 50,000 people every day. The worldwide emergence of multidrug resistant Escherichia coli and many other β-lactamase producers has become a major therapeutic problem. The development of bacterial resistance to presently available antibiotics has necessitated the search for new antibacterial agents. Many infectious diseases have been known to be treated with herbal remedies throughout the history of mankind. In rural and backward areas of India, several plants are commonly used as herbal medicine for the treatment of infectious diseases. Hibiscus rosa-sinensis commonly found plants were screened for potential antibacterial activity. The present study was conducted to evaluate the In-vitro and In-vivo evaluation of the antibacterial activity of ethanolic and aqueous leaf extract of Hibiscus rosa-sinensis in mice. In-vitro activity of ethanolic and aqueous leaf extract of Hibiscus rosa-sinensis at concentrations of 50, 100 and 200 mg/ml was tested against E. coli and Staphylococcus aureus using the agar well diffusion method and was compared with penicillin and gentamicin. Ethanolic leaf extracts showed higher inhibitory activity compared to aqueous extract at 50 and 100 mg/ml concentrations. MIC value of Hibiscus rosasinensis was found to be 2.5 and 5 mg respectively.
Preliminary screening of antibacterial activity using crude extracts of Hibiscus rosa sinensis
Tropical Life Sciences …, 2009
Hibiscus rosa sinensis, a member of the Malvaceae family, is widely cultivated in the tropics as an ornamental plant. It is often planted as a fence or hedge plant, and has several forms of flowers with varying colours. It is also used in traditional medicine to induce abortion, ease menstrual cramps, assist in childbirth and relieve headache, fever and inflammation. In this study, we evaluated the antibacterial activity of H. rosa sinesis extract using a disc diffusion method. Crude petroleum ether extract, ethyl acetate extract and methanol extract from the leaves, stems and flowers of the plant were prepared using a cold extraction technique. These extracts were tested at concentrations ranging from 4 mg/disc to 0.017 mg/disc against methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia. The petroleum ether extract from the leaves, stems and flowers and methanol extract from the leaves showed i...
In-vitro Antimicrobial Activities of different Hibiscus Leave Extract
International Journal of Scientific Research in Science, Engineering and Technology, 2021
Solvent preparations of the different Hibiscus leaf have been mostly used in folk medicine for various purposes. In present study in this paper we have studied the antimicrobial activities of the leaf extract of Hibiscus rosa sinersis, H. aculeatus, H. sabdariffa, H. mutabillis and H. ficulness. We have evaluated the antimicrobial activity of the leaf extracts of all five Hibiscus sp. by agar well diffusion method. In the first screening all of the bacterial and fungal showed varying degrees of sensitivity to the Hibiscus leaf extract. Minimum antimicrobial activity was found by the petroleum either extracts. In among all four different solvent extract shows Ethyl acetate extract was found to be most favorable and suitable solvent for extraction and give maximum results in antimicrobial activities.
Journal of Microbiology and Antimicrobials, 2014
Hibiscus diversifolius which is widely distributed in Kenya was investigated for its antibacterial effect against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus. The leaves stem and root of the plant was extracted using aqueous and methanol as solvents. Phytochemical screening was also carried out to determine the phytochemical constituents present in the various parts of the plants used. The results show that both aqueous and methanolic extracts of the different plant parts had antibacterial activity against the various microbes tested. Phytochemical screening revealed the presence of alkaloids, flavonoids, sterols, saponins, terpenoids and cardiac glycosides while tannins and steroids were lacking in all the extracts.
Antimicrobial and Antioxidant activity of Hibiscus Sabdariffa. linn (Roselle
international journal of pharmacy research & Technology, 2021
More than 300 species of Hibiscus Sabdariffa Linn. are present all over the world. Roselle is an annual herbaceous plant obtained from Hibiscus Sabdarifa Linn. Belonging to the family Malvaceae and Genus Hibiscus. It is also known as Aambadi in Marathi. Roselle is used for various medicinal purposes. Hibiscus Sabdariffa uses for making jellies, sauces, syrups, jams as well as use as a flavoring and colouring agent in drinks. the calyx is used as a beverage that helps to lower body temperature. Roselle is also used as a diuretic, laxative, anthelmintic. Also, use to lower blood pressure.15%-30% plant is made up of plant acids like citric acid, tartaric acid. Roselle mainly contains alkaloids, quercetin, L-ascorbic acid, anthocyanin, Beta-carotene. In this article, we focused on the antioxidant and antimicrobial activities of the rosella flower and we found that the alcoholic extract of the plant shows good antioxidant and antimicrobial activity as compared to the aqueous extract at very low concentration. The antioxidant activity was performed by using the DPPH and ferric thiocyanate activity model while the antimicrobial activity is done by using E.coli and S. aureus which shows a good result when they are evaluated.
Antibacterial activity, phytochemical screening and cytotoxicity of Hibiscus sabdariffa (calyx)
2016
The purpose of the paper was to investigate the in-vitro antibacterial activity, preliminary phytochemical screening and cytotoxicity [3-(4,5-dimethyl thiazol-2-yl)-2, 5-diphenyltetrazolium bromide-MTT assay] of methanol extract of Hibiscus sabdariffa L. (calyxes). The methanol extract of H. sabdariffa was tested against 11 clinical isolates bacteria and 3 standard bacteria, that is: 5 Gram positive bacteria (Corynebacterium diphtheria, Staphylococcus aureus, Enterococcus faecalis,Listeria monocytogenes and Bacillus cereus), 9 Gram negative bacteria (Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Pseudomonas aeruginosa (ATCC 27853), Serratia marcescens, Escherichia coli, Escherichia coli (ATCC 25922), Klebsiella pneumonia and Klebsiella pneumoniae (ATCC 70063) using the cup-plate agar diffusion method, preliminary phytochemical screening and cytotoxicity (MTT assay) was also performed with different concentrations (500, 250 and 125 μg/ml) and compared to the reference ...
Crude preparations of the different parts of Hibiscus rosa-sinensis have been traditionally used in folk medicine for various purposes. In the present study, we have evaluated the antibacterial activity of the extracts of H. rosa-sinensis leaves and flowers against some clinical isolates of bacteria by simple agar-well diffusion and bacteriological enumeration method. In the preliminary screening experiment, all of the bacterial isolates showed varying degrees of sensitivity to the flower extracts excluding Klebsiella pneumoniae. We found Staphylococcus aureus, a Gram-positive bacterium as the most sensitive to the extracts of flowers at the applied doses of 50 and 100 mg/well. On the other hand, most of the tested bacterial isolates were resistant to the extracts of leaves excluding S. aureus and Salmonella typhimurium. In bacteriological enumeration study, all of the bacterial isolates showed substantial extent of sensitivity to the different extracts used. Our findings clearly demonstrate that the flower extracts of H. rosa-sinensis had stronger antibacterial effects than that of leaves and raises the possibility of using the extracts as antibacterial agents in treating pathological conditions caused by S. aureus and S. typhimurium infection. Although the effect of the H. rosa-sinensis flowers and leaves against some pathogenic bacteria in vitro is promising, further microbiological and pharmacological studies will be required before starting clinical trials.
Antioxidant and antibacterial activities of hibiscus
Antioxidant activity, antibacterial properties, color and FT-IR spectral analysis of flowers belonging to hibiscus and Cassia species were investigated. Radical scavenging activity of sample extracts were determined based on the percent inhibition of DPPH and ferric reducing antioxidant power (FRAP) assays. Total phenolics were estimated based on the Folin–Ciocalteu method, while, vanillin–HCl and aluminum chloride methods were employed to estimate total tannins and flavo-noids in the sample extracts, respectively. To determine total flavonols and anthocyanin contents, spectrophotometric method was employed. For antibacterial activities, modified agar disk diffusion method was adopted. Color analysis was performed using a colorimeter, while functional groups of compounds were identified using a FTIR-spectrophotometer. Results showed both the flower extracts to encompass high amount of antioxidant compounds and exhibit significant antioxidant activities, which depended on extraction solvents. Ethanolic extracts of Cassia had high total pheno-lic, total flavonoid and total flavonol content, and showed highest activity for inhibition of DPPH, while aqueous extract of hibiscus had high tannin and anthocyanin contents, and showed high ferric reducing antioxidant power. With regard to antimicrobial activity, aqueous and ethanolic extracts of hibiscus inhibited the growth of food-borne pathogens such as Salmonella typhimurium and Staphylococcus aureus, while in Cassia the growth of Bacillus cereus and Klebsiella pneumoniae was inhibited. Compared to Cassia, color analysis of hibiscus showed lower chroma and hue angle values. FTIR spectra of both flowers were comparable and showed the presence of polysaccharides, suberin and triglycerides. Our results indicate the potential of exploiting these two flowers as a source of natural food preservative or colorant, while developing novel functional foods. ª 2012 Production and hosting by Elsevier B.V. on behalf of King Saud University.