Antidiabetic, renal/hepatic/pancreas/cardiac protective and antioxidant potential of methanol/dichloromethane extract of Albizzia Lebbeck Benth. stem bark (ALEx) on streptozotocin induced diabetic rats (original) (raw)
Related papers
Gmelina arborea may be the good remedy for the treatment of diabetes mellitus. In this study, the oral administration of Luffa acutancula and Gmelina arborea showed the hypoglycemic activity and it could exert a beneficial action against biochemical alterations caused by the streptozotocin. Diabetes mellitus is a disease that affects more than 100 million people and may attain about five times more subjects in the next 10 years. In the search for new compounds, and within the exploratioration of natural resources, the hypoglycemic effect of plants which are reputed antidiabetic. The present study is carried out to identify new potential antidiabetic compounds of Luffa acutancula and Gmelina arborea. In the present study the antioxidative potential of Luffa acutancula and Gmelina arborea was assessed in streptozotocin induced diabetic rats. Lipid peroxide levels were also measured in normal, diabetic and treated animals. Malondialdehyde (MDA) levels were significantly higher and antioxidant activity was found low in diabetic groups as compared to the control groups, and significant alteration in both the MDA levels and antioxidant activity was also observed when the above herbal hypoglycemic agents were given to diabetic rats. On the basis of our results we conclude that Luffa acutancula and Gmelina arborea are not only useful in controlling the lipid peroxide levels but are also helpful in further strengthening the antioxidant potential. The probable mechanism by which Luffa acutancula and Gmelina arborea exerts its protective action against streptozotocininduced pancreatic metabolic alterations could be by the stimulation of pancreatic regeneration through an improved synthesis of protein or accelerated detoxification and exertion. Furthermore, comprehensive chemical and pharmacological research is required to reveal the mechanism of the anti-diabetic potential of Luffa acutancula and Gmelina arborea.
Gmelina arborea may be the good remedy for the treatment of diabetes mellitus. In this study, the oral administration of Luffa acutancula and Gmelina arborea showed the hypoglycemic activity and it could exert a beneficial action against biochemical alterations caused by the streptozotocin. Diabetes mellitus is a disease that affects more than 100 million people and may attain about five times more subjects in the next 10 years. In the search for new compounds, and within the exploratioration of natural resources, the hypoglycemic effect of plants which are reputed antidiabetic. The present study is carried out to identify new potential antidiabetic compounds of Luffa acutancula and Gmelina arborea. In the present study the antioxidative potential of Luffa acutancula and Gmelina arborea was assessed in streptozotocin induced diabetic rats. Lipid peroxide levels were also measured in normal, diabetic and treated animals. Malondialdehyde (MDA) levels were significantly higher and antioxidant activity was found low in diabetic groups as compared to the control groups, and significant alteration in both the MDA levels and antioxidant activity was also observed when the above herbal hypoglycemic agents were given to diabetic rats. On the basis of our results we conclude that Luffa acutancula and Gmelina arborea are not only useful in controlling the lipid peroxide levels but are also helpful in further strengthening the antioxidant potential. The probable mechanism by which Luffa acutancula and Gmelina arborea exerts its protective action against streptozotocininduced pancreatic metabolic alterations could be by the stimulation of pancreatic regeneration through an improved synthesis of protein or accelerated detoxification and exertion. Furthermore, comprehensive chemical and pharmacological research is required to reveal the mechanism of the anti-diabetic potential of Luffa acutancula and Gmelina arborea.
ANTIDIABETIC POTENTIAL OF HERBAL EXTRACTS STUDIED IN (STZ) STREPTOZOTOCIN INDUCED DIABETIC RAT
Synthetic drugs are commonly used in the present day with the advancement in chemical technology awareness about its side effects is slowly triggering the use of non-synthetic curatives. The curative properties of these drugs are due to the complex chemical substances present as secondary plant metabolites in one or more parts of these plants. Diabetes Mellitus is a clinical condition characterized by increased blood glucose level due to the insufficient or inefficient insulin. Streptozotocin is to induce hyperglycemic condition. Gymnema sylvestre, Eugenia jambolana, Tulsi (Ocimum sanctum) and Bittergourd (Momordica charantia) are a popular remedy for the treatment of diabetes mellitus. In this study, oral administration of Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia showed the hypoglycemic activity and it could exert a beneficial action against biochemical and histopathological alterations caused by streptozotocin. In the present study the antioxidative potential of Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia was assessed in streptozotocin induced diabetic rats. Lipid peroxide levels were also measured in normal, diabetic and treated animals. Malondialdehyde (MDA) levels were significantly higher and antioxidant activity was found low in diabetic groups as compared to the control groups, and significant alteration in both the MDA levels and antioxidant activity was also observed when the above herbal hypoglycemic agents were given to diabetic rats. On the basis of our results we conclude that Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia are not only useful in controlling the lipid peroxide levels but are also helpful in further strengthening the antioxidant potential.The probable mechanism by which Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia exerts its protective action against streptozotocininduced pancreatic metabolic alterations could be by the stimulation of pancreatic regeneration through an improved synthesis of protein or accelerated detoxification and exertion. Furthermore, comprehensive chemical and pharmacological research is required to reveal the mechanism of the anti-diabetic potential of Gymnema sylvestre, Eugenia jambolana, Ocimum sanctum and Momordica charantia.
2019
Antidiabetic potency of locally produced antidiabetic herbal preparations (ADHPs) are unknown. In this study, a pharmacological screening of antihyperglycemic potency of locally available six ADHPs were evaluated on streptozotocin (STZ) induced type 2 diabetic rat models. ADHPs were administered to the diabetic rat and blood glucose levels were monitored before (0 day) and after administration of ADHPs (at 14th day and 28th day) by measuring serum glucose concentration using oxidase method. Serum glucose levels in different groups of ADHP treated rats were compared against baseline glucose levels and water control and reference drug by t test. Serum glucose levels were found to be similar at baseline in all groups against water control and reference drug. The administration of herbal preparations except ADHP-3 and ADHP-5 resulted in a non-significant reduction of blood glucose level of the diabetic rats when compared with diabetic rats that received no treatment at 14th day or compa...
International Journal of Pharmacology and Toxicology, 2013
Back ground: Evidences suggest that oxidative stress may play an important role in the etiology of diabetes and diabetic complications. The free radicals can be encountered by the conventional treatment of diabetes. Alternatively, some plants and trace elements may be proven to bring desirable results including Coriander and Vanadyl sulfate. To produce hypoglycemic and anti oxidative effects, expensive and huge amount of these are required. Objective: An innovative method should be developed in which the Vanadyl sulfate may be up taken by coriander so that it must render it non toxic to produce hypoglycemic and anti oxidative effects in diabetic rats. Material & methods: 150 albino rats were employed. 25 rats were kept normal (Group A). 125 made diabetic by STZ injection. Total 05 groups were identified and developed; each comprised of 25 rats. These were treated by Vanadyl sulfate (Group B), coriander only (Group C), Vanadyl sulfate up taken by coriander (V+C) (Group D), insulin treated (Group E) and last one did not receive any treatment (Group F). Results: The hypoglycemic effects and total antioxidant activity produced by the Vanadyl sulfate uptaken coriander are compatible than that of the insulin with negligible difference. All the other employed groups in the study did not reveal marked hypoglycemic activity. Pharmacological studies of Vanadyl sulfate uptaken herb demonstrated no deleterious effects. Conclusion: The detoxification of vanadyl sulfate has been achieved through possible auto oxidation of this valuable trace element by coriander. Vanadyl sulfate uptaken by coriander acts as antidiabetic agent and shows a considerable insulinomimetic and total antioxidant activity in the Diabetic rats.
Alhagi maurorum (camel thorn plant) is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. No previous study has been conducted before on A. maurorum extracts as an antioxidative stress and/or antidiabetic herb in STZ-induced DM in rats. Therefore, four groups of rats were allocated as control (C), STZ-induced DM (D), and STZ-induced DM supplemented with 300 mg/kg BW of either aqueous extract (WE) or ethanolic extract (EE) of A. maurorum. The plasma levels of glucose, TG, TC, LDL-C and VLDL-C, MDA, and bilirubin and the activities of transaminases and GR were significantly increased in the diabetic group. Also, diabetic rats showed severe glucose intolerance and histopathological changes in their livers. In addition, levels of insulin, total proteins, GSH, and HDL-C and the activities of SOD, GPx, and GST were significantly decreased in the diabetic rats compared to those of the control group. The ingestion of A. maurorum extracts lowered the blood glucose levels during the OGTT compared to the diabetic rats and restored all tested parameters to their normal levels with the exception of insulin level that could not be restored. It is concluded that A. maurorum extracts decreased elevated blood glucose levels and hyperlipidemia and suppressed oxidative stress caused by diabetes mellitus in rats.
Evaluation of Antioxidants in the Kidney of Streptozotocin Induced Diabetic Rats
Indian Journal of Clinical Biochemistry, 2013
Diabetes mellitus is one of the most common endocrine metabolic disorders. Dual endocrine deficits of impaired insulin action (insulin resistance) and inadequate insulin secretion create an environment of chronic hyperglycemia and general metabolic disarray. Oxidative stress plays an important role in diabetic pathogenesis. Oxidative stress induced by streptozotocin (STZ) has been shown to damage pancreatic beta cell and produce hyperglycemia in rats. The present study was made to evaluate the antioxidant activity of ethanolic extract of the Evolvulus alsinoides in STZ induced rats. The antioxidant activities were done by using standard protocols. For histopathological analysis, the pancreatic tissues of all experimental groups were fixed with 10 % formalin for 24 h then the samples were stained with hematoxylin-eosin for the microscopic observation. Our results showed the significant decrease in lipid peroxidation and increases in the antioxidant (both enzymatic and nonenzymatic) levels after treatment with standard as well as the E. alsinoides. There is no significant difference between control and plant alone group rats. The histopathology reports also revealed non-toxic effect and protective effect of E. alsinoides in the kidney of STZ induced diabetic rats. Our result indicated that the E. alsinoides extract effectively increased the antioxidant level thereby it prevents oxidative stress during diabetes mellitus and also it showed the protective effect on kidney of STZ induced rats. Hence it can be used to maintain the antioxidant level during diabetes mellitus.
Digest Journal of Nanomaterials and Biostructures
Our investigations explore that herbal medicine ethanolic extract of Aloe vera exibits antihyperglycemic, and antioxidant effects in streptozotocin (STZ) induced diabetic rats. Aloe vera (AV) leaf gel extract administered orally to different groups of rat at a dose of 300 mg/kg body weight. Five groups (n = 6) follows, control rats, control + Aloe vera, diabetic rats (STZ 40 mg/kg body weight), diabetic + Aloe vera, diabetic + glibenclamide. The experimental period was 30 days. In diabetic rats body weights were decreased and blood glucose levels were increased when compared to control rats. The diabetic rats exhibited lower activity levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) in hepatic and renal tissues as compared with normal rats. The activities of SOD, CAT, GPx and GR were increased in hepatic and renal tissue of AV extract treated diabetic rats. The increased level of lipid peroxidation in diabetic rats was ...
The effects of various extracts of Ocimum basilicum leaf on biochemical indices of organ damage and oxidative stress status of streptozotocin-induced diabetic rats were examined. Oral administration of 200mg/kg of aqueous, methanolic and petroleum ether extracts of the leaf for 35 days resulted in a significant (P<0.05) reduction in thiobarbituric acid reactive substances (TBARS) and an increase in catalase (CAT) and superoxide dismutase (SOD) activities in streptozotocin-induced diabetic rats from diabetic levels. The leaf extracts brought about a significant (P>0.05) increase in serum protein and albumin as well as decreases in urea and creatinine levels of STZ -induced diabetic rats compared with diabetic control levels. The Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) levels increased significantly (P>0.05) in diabetic control group. The extracts caused a significant reduction in levels of AST and ALT in treated diabetic groups and maintained the normal level observed in normal rats. In this study a significant decrease in PCV was observed in diabetic control group and increase in the PCV in rats given extracts. It was concluded that the extracts have in vivo antioxidant, hepatoprotective and nephroprotective effects in STZ -induced diabetic rats. These results support its traditional use in the management of diabetes and cardiovascular diseases.
The phytobiotics used in this study are turmeric (Curcuma longa) and bitters (extracts from leaves of Vernonia amygdalin). The effects on oxidative stress level and hematological parameters in streptozotocin-induced diabetic Wistar rats treated with either insulin, ethanol extracts of Curcuma longa (turmeric) or Vernonia amygdalina (bitters) was investigated for 28 days. Diabetes was induced in the animals using a single dose of streptozotocin (50 mg/kg), intraperitoneally. Confirmation of diabetes was made 48 h post induction with STZ. Thirty male Wistar rats were divided into six groups of five comprising of Group 1 (normal control), Group 2 (diabetic), Group 3 (insulin treated), Group 4 (turmeric, 400 mg/kg), Group 5 (Curcuma longa, 800 mg/kg), and Group 6 (bitters, 400 mg/kg). At the end of the treatment (28 days), blood was collected from the retrobulbar plexus for various biochemical analyses and animals were humanely sacrificed. Results of blood glucose revealed that at Day 0 and 7, Groups 2, 3, 4, 5 and 6 had a significantly (p <0.001) high blood glucose concentration which gradually reduced and at day 28 was significantly (p<0.001) reduced in Groups 3, 4, 5 and 6. There were significant changes in WBC count, lymphocyte count, platelet count and granulocyte count among the groups while RBC, Hb, PCV, MCV, MCH and MCHC showed no significant difference in all the groups. The GSPx, SOD and Catalase levels in Groups 3, 4, 5 and 6 were significantly (p<0.001) increased when compared to Group 2 which was significantly decreased (P<0.001). MDA concentration was significantly increased (P<0.001) in Group 2 compared to normal control group, Groups 3, 4, 5 and 6. These effects were seen to be ameliorative to varying degrees in the insulin-and extract-treated groups. Turmeric and bitters have been demonstrated in this study to significantly decrease oxidative stress levels and improved altered hematological functions to appreciable levels comparable to normal control and insulin-treated diabetic groups. Specifically, C. longa extract (turmeric) has proved to be a potent antihyperglycemia, anti-anaemic and antioxidant extract. Cite This Article: Etim Affiong O., Ani Elemi J., Igajah Collins N., and Nwaehujor Chinaka O., " Effects of Phytobiotics on Oxidative Stress and Hematology in Streptozotocin (STZ)-induced Diabetic Wistar Rats: A Comparative Study.