Antioxidant Activity of Selected Medicinal Plants (original) (raw)
Related papers
Screening of plant extracts for antioxidant properties
2011
Antioxidant properties of total methanol extracts from 54 species of 30 families were studied. DPPH (1,1-diphenyl-2-picryl hydrazyl) radical was used for evaluation of free radical scavenging. Among tested species, the extracts of Rumex crispus (radix), Rubus occidentalis (folia), Rumex alpinus (radix), Euphorbia helioscopia (herba) and Rubus idaeus (folia), exhibited the strongest activity. Signifi cant radical scavenging activity was found also in the extracts of Echium vulgare (herba), Plantago arenaria (herba), Viola tricolor (folia), Pyrus communis (folia), Sideritis montana (folia), Betula pendula (folia), Achillea critmifolia (herba), Santolina rosmarinifolia (herba), Morus alba (folia) and Erigeron canadensis (herba). Moderate activity was shown by extracts of Forsythia (folia), Bryonia alba (folia), Hepatica nobilis (folia), Plantago cornuti (folia), Maclura cochinchinensis (folia), Cihorium inhybus (herba) and Caltha palustris (herba).
Determining the Antioxidant Property of Plant Extracts: A Laboratory Exercise
The Asian Journal of …, 2011
Vegetables and fruits are good sources of free radical scavengers or antioxidants. To evaluate the free radical scavenging activity of a plant extract, a few simple steps using the 1,1-diphenyl-2picryl hydrazyl (DPPH) assay can be followed. Any plant part can be processed to produce crude extracts which can be used for testing. Using this technique, a small class of tertiary students determined the antioxidant property of ethanolic crude leaf extracts of some endemic and indigenous plants. The plants used were Ardisia pyramidalis Roth (Myrsinaceae), Baccaurea tetrandra (Baill.) Mull.Arg. (Phyllanthaceae), Chisocheton pentandrus (Blanco) Merr. (Meliaceae), Ficus septica Burm. (Moraceae), Parameria laevigata (Juss.) Moldenke (Apocynaceae), Parartocarpus venenosus (Zoll. & Moritzi) Becc. (Moraceae), Streptocaulon baumii Decne. (Asclepiadaceae), Uncaria perrottetii (A. Rich) Merr. (Rubiaceae), and Voacanga globosa (Blanco) Merr. (Apocynaceae). Crude leaf extracts of U. perrottetti and B. tetrandra were observed to possess a high free radical scavenging activity with values beyond 90% of that of gallic acid. These were fractionated further, and subsequent assays showed that ethyl acetate fractions for both plants had high free radical scavenging activity indicating that they contain potential chemopreventive agents against many diseases such as cancer, cardiovascular disorders and aging. Free radical scavenging activities demonstrated by leaf extracts of A. pyramidalis and C. pentandrus did not reach 70% of that of gallic acid. All the rest of the plant extracts showed very low or no free radical scavenging activity.
Screening of thirteen medicinal plant extracts for antioxidant activity
2010
Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical induced oxidative stress. A variety of free radical scavenging antioxidants exist within the body which many of them are derived from dietary sources like fruits, vegetables and teas. In this study the antioxidant activity and radical scavenging activity of methanolic extracts of selected plant materials, traditionally used by Iranian population as folk remedies was evaluated against linoleic acid peroxidation and 2,2diphenyl-1-picrylhydrazyl radical. The antioxidant activity expressed as IC 50 ranged from1.28 ng/ml in Biebresteinia multifida to 63.48 ng/ml in Polypodium vulgare. Radical scavenging activities expressed as IC 50 varied from 1.83 µg/ml in Salix sp. to 187.88 µg/ml in Allium hirtifolium.
Screening of various botanical extracts for antioxidant activity using DPPH free radical method
African Journal of Traditional, Complementary and Alternative Medicines, 2013
Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The overall antioxidant activity of grape seeds (Vitis vinifera) was the strongest, followed in descending order by soybean (Glycin max) and tamarind (Tamarindus indica). The seeds extract of Vitis vinifera, Glycin max and Tamarindus indica showed 85.61%, 83.45% and 79.26%, DPPH scavenging activity respectively.
Screening of medicinal plant extracts for antioxidant activity
Journal of Medicinal Plants …, 2009
The methanolic crude extracts of Desmodium gangeticum (Linn.), Eclipta alba (Linn.) Ocimum sanctum (Linn.), Piper longum (Linn.), Solanum nigrum (Linn.) and Amaranthus caudatus (Linn.) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical. The overall antioxidant activity of D. gangeticum was found to be the strongest, followed in descending order by A. caudatus, S. nigrum, P. longum, E. alba and O. sanctum. The IC 50 values of the extracts ranged between 0.05 ± 0 and 0.19 ± 0 mg/l . The ascorbic acid levels varied from 3.86 ± 0.20 to 21.33 ± 1.49 mg/100g and the carotenoids content were observed between 9.0 ± 0.24 to 24 ± 1.16 mg/100g in plant extracts. The highest total phenols content were found to be in O. sanctum (Linn.) with the value 48.93 ± 0.24 mg/g. The present study reveals that the selected plants would exert several beneficial effects by virtue of their antioxidant activity and could be harnessed as drug formulation.
Pak J Bot, 2011
The active parts of 11 medicinal plants were analyzed for physico-chemical evaluation, phytochemical determination and antioxidant activity. The physico-chemical evaluation revealed that highest water soluble extractive was from Origanum vulgare (38%), highest chloroform extractive was from Psoralea corylifolia (21%); highest ethanolic extractive was that of Acorus calamus (11%) and the highest hexane extractive value was for Arnebia nobilis (9.8%). The total ash content evaluation indicated that Achillea millefolium yielded (20.2 %) and Rauvolfia serpentina yielded (41.6%); these values are much higher than the standard ash values for these plants indicating that these drugs are highly adulterated and substandard. The highest essential oil was yielded by Acorus calamus (3.2%). The highest saponin percentage was analyzed in Acorus calamus (8.9%), while the alkaloids percentage was determined at 21% in Peganum harmala. Among all the plants assessed for DPPH free radical scavenging activity, the maximum activity was shown by Paeonia emodi (85.8%), followed by Achillea millefolium (81.7%) and Origanum vulgare (80.3%).
study on the antioxidant property of selected medicinal plants
The Clitoria ternatea, Solanum nigrum and Aloe vera leaves were extracted serially by the solvents of increasing polarity (petroleum ether, chloroform and ethanol) were tested for their free radical scavenging activity against DPPH. The petroleum ether extracts of Clitoria ternatea, Solanum nigrum and Aloe vera leaves were the most effective scavenging of DPPH. These plant extracts were also analyzed for the activities of selected enzymic antioxidants such as catalase, polyphenol oxidase and the non-enzymic antioxidants were ascorbic acid and total phenols. The results showed the leaves of Solanum nigrum possess higher levels of antioxidants.
Pakistan Journal of Pharmaceutical Sciences, 2013
The present research investigation was aimed at the evaluation of antioxidant activities of methanolic (70%) extracts of whole plant of Pentanema vestitum and fruits of Pistacia integerrima, Withiana somniferra and Withiana coagulans on scavenging of 2, 2-diphenyl-2-picrylhydrazyl (DPPH) free radical. The rank of order of free antioxidant activity of the selected plants was; P. integerrima > P. vestitum > W. somniferra > W. coagulans as compared to standard Ascorbic acid. P. integerrima showed significantly higher activity at all concentrations as compared to Ascorbic acid at P<0.05. The percent inhibition caused by P. integerrima at lowest concentration (40 ppm) was 68.16±0.5 and that of Ascorbic acid was 62.00±0.5. The IC50 value of P. integerrima was 5.75ppm as compared to ascorbic acid having 15.09 ppm. The percent inhibition at all concentrations caused by P. vestitum was not significantly different from Ascorbic acid at P<0.05. The IC50 value of P. vestitum was 13.00ppm and that of Ascorbic acid was 15.09 ppm. The percent inhibitions caused by W. somniferra (IC50=46.85 ppm) and W. coagulans (IC50=84.40 ppm) were most significantly lower than Ascorbic acid at P<0.05. It is inferred from the current study that the methanolic (70%) extracts of the P. integerrima and P. vestitum could be used in preparation of potent antioxidant drugs.
Screening of the Antioxidant Activity of Some Medicinal Plants
Korean Journal of Food Science and Technology, 2004
Antioxidant activities of water extracts of 20 medicinal plants (1 mg/mL) on peroxidation of linolic acid were evaluated by thiocyanate method, among which 11 showed strong antioxidant activity (> 70%). Higher hydroxy radical scavenging activity (> 60%) were shown in Cornus officinalis, Acanthopanax sessiliflorus, and Epimedium koreanum than the other plants. Epimedium koreanum than the other plants extract showed highest superoxide radical scavenging activity (42%). Total polyphenol contents ranged from 2.6 (Polygonatum odoratum)~81.2 (Epimedium koreanum) mg/g. Direct correlation between the antioxidant activity and polyphenol content (r=0.8) was established through simple regression analysis. IC 50 for selected four plant extracts, showing highest polyphenol contents and antioxidant activities, were significantly higher than positive control. Total antioxidant activity of vitamin c was significantly lower than those of Acanthopanax sessiliflorus, Epimedium koreanum, and Erythrina variegata. Superoxide radical scavenging activity of Acanthopanax sessiliflorus was similar to BHA. Results suggest water extracts of some medicinal plants could be potential candidates for natural antioxidants.