Variability in essential oil composition of Turkish basils (Ocimum basilicum L.) (original) (raw)
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Essential oil composition of five Basil cultivars (Ocimum basilicum) from Albania
Macedonian Pharmaceutical Bulletin, 2015
Basil (Ocimum basilicum L., fam. Lamiaceae) is an important medicinal and aromatic plant with very wide range of uses. This paper presents qualitative and quantitative analyses of essential oils obtained from five types of Italian basil cultivars: Napolitan, Red basil, Fino Verde, Limonez, and Genoveze culltivated in Albania and collected during the summer 2012. The hydrodistilled BEO (Basil essential oil) content ranged from 0.11% to 3.40%. Within the total of 65 identified compounds with GC/FID/MS, nine were considered as predominant (1,8-cineole, linalool, cis-thujone, methyl chavicol, eugenol, trans-(E)-caryophyllene, trans-(α)-bergamotene, germacrene D, and epi-α-cadinol) representing 49.20 to 85.43% of the components in the analysed essential oils. In all cultivars, linalool was detected as the most abundant component (36.20-46.59%). BEO’s from all five cultivars differ in their chemical composition but generally conform to EO’s from Sweet Basil grown in the Medditeranean regi...
Essential-Oil Variability in a Collection ofOcimum basilicumL. (Basil) Cultivars
Chemistry & Biodiversity, 2016
Ocimum basilicum L. (Lamiaceae) is an aromatic plant of great tradition in the Mediterranean area. Its economic importance is growing up determining an expansion of cultivation. This paper evaluated the morphological traits, the chemical profiles, and antibacterial activity of 21 cultivars of basil belonging to ʻGenoveseʼ, ʻNapoletanoʼ, and ʻPurple basilʼ types. The cultivars were characterized by different growth rate and morphological traits. The chemical composition of the oils analyzed by GC and GC/MS analysis, supported by the PCA analysis, underlined the strong influence of chemotype. It is noteworthy that estragole, never present in Genovese and purple basil types, occurred in Napoletano type. The high presence of eugenol, methyl eugenol, and linalool in the majority of cultivars, belonging both to Genovese and to Napoletano types was registered. Of great interest resulted the composition of the purple basil ʻOpalʼ. All the samples tested exhibited similar antibiotic profiles with moderate antibacterial activity. The results enhanced the importance of determination of essential-oil profile in the selection of cultivars characterized by diverse morphological traits and are useful for different purposes.
Cultivation Site Influence of Essential Oils Component in Purple and Green Basil
2023
Sweet basil (Ocimum basilicum L.) is an herbaceous annual aromatic herb belongs to the Lamiaceae family. The compositions of essential oil are the main parameters for assessing quality of basil for different food, pharmaceutical, and chemical industries. On the other hand, secondary metabolites in basil are affected by the interaction of location and genetics. In this study essential oil composition of two basil cultivars (green and purple) in different cultivation site were determined. The experiment took place in Isfahan and Marand cities with different climate, edaphic and elevation factors. Plants were harvested at flowering stage and transported to the laboratory and samples dried at shade condition. Essential oils were obtained by hydro-distillation and analysis of essential oils was carried out by GC and GC-MS technics. There were differences among constituents in the essential oil content from the basil cultivars at two locations. For green basil, the major constituent of the essential oil from aerial parts were Geraniol (36.21%, in Marand region), nerol (27.02%) and methyl-chavicol (18.79%) in Isfahan region. Green basil, grown in the Isfahan region had higher concentrations of essential oils component than in the Marand region. For purple basil, methyl-chavicol (54.54%) and linalool (26.10%) in Marand region and (E)-β-ocimene (3.86%) in Isfahan regain were the highest essential oil components. According to our results, location could affect the efficacy of production for use of basil in drug industries.
Advances in Bioscience and Biotechnology, 2016
Sweet basil (Ocimum basilicum L.) project that is being conducted at the National Oilseed Processing Research Institute (NOPRI) aims at exploiting the genetic diversity and associated phenotypic variation in the investigated basil germplasm and evaluating key factors such as variety, plant ontogeny and plant parts that affect yield, chemical constituents, aroma and flavor of essential oils. This paper discussed the variability inessential oils content and chemical constituents of the aerial plant parts of the basil. An open-air pot experiment was carried out using three accessions provided by Agricultural Research Corporation-Genetic Resources Unit, Sudan. The essential oils were hydro distilled from the aerial parts, flowers, leaves, stems, using Clevenger apparatus. The chemical constituents of the essential oils were determined by Gas Chromatography-Mass Spectrometry (GC-MS). As the hydro distillation yielded no oil from the stem, we present only the results for leaves and flowers. The results revealed that the essential oil content varied with a range of 0.29% to 0.33% for flowers and 0.32% to 0.48% for leaves. As usual, the content of essential oils was higher in leaves than in flowers. The Silate-Egyptian accession had the lowest essential oil content obtained from leaves and flowers. However, South Darfur accession from Sudan had the highest oil content but did not significantly differ from Kennana accession. Several oil components were identified based upon comparison of their mass spectral data with those of reference compounds published in literature or stored in a computer library. The combined GC-MS * Corresponding author. Y. A. A. Aburigal et al. 184 analysis showed that methyl eugenol was the major constituent of the essential oil of Kennana accession. While the major constituent of essential oil of South-Darfur and Silate-Egyptian accessions were germacrene and linalool, respectively.
RAPD and essential oil characterization of Turkish basil (Ocimum basilicum L.)
Plant Systematics and Evolution, 2014
Sweet basil (Ocimum basilicum L., Lamiaceae), an important medicinal plant and culinary herb due to its delicate aroma and fragrance, shows great variation in both morphology and essential oil components. Genetic variation among basil accessions in Turkey has not been extensively examined with molecular markers. Genetic diversity was determined using random amplified polymorphic DNA (RAPD) markers of 14 genotypes of basil. A total of 375 bands were obtained from the RAPD analysis, and 273 of them (70.3 %) were polymorphic. The RAPD analysis allowed the grouping of samples into two main clusters. Genetic similarity values among the basil genotypes ranged between 0.46 and 0.87. Considerable genetic diversity was determined among basil genotypes. Essential oils were obtained by hydro-distillation and were characterized by gas chromatography. A total of 17 chemical components were identified. The evaluated genotypes of O. basilicum can be classified into seven chemotypes: (1) Linalool/methyl eugenol (25K), and Methyl chavicol/linalool (Let). The chemical variability obtained from the essential oil composition of the genotypes in the study was remarkable. The chemical characterization of genotypes 10L and 17 was rich in citral (42.17 and 44.80 %) and methyl chavicol (30.56 and 32.03 %). Citral/methyl chavicol can be assessed as a new chemotype of basil cultivated in Turkey. The basil genotypes were grouped into two major clusters for both the RAPD analysis and chemical characterization with very few exceptions (genotype n. 6). A correlation analysis of the genetic distance matrix and the Euclidian distance matrix showed relatively low values (r = -0.40). The results demonstrated a certain degree of correspondence between chemical and molecular data.
Advances in Bioscience and Biotechnology, 2016
Sweet basil (Ocimum basilicum L.) project that is being conducted at the National Oilseed Processing Research Institute (NOPRI) aims at exploiting the genetic diversity and associated phenotypic variation in the investigated basil germplasm and evaluating key factors such as variety, plant ontogeny and plant parts that affect yield, chemical constituents, aroma and flavor of essential oils. This paper discussed the variability inessential oils content and chemical constituents of the aerial plant parts of the basil. An open-air pot experiment was carried out using three accessions provided by Agricultural Research Corporation-Genetic Resources Unit, Sudan. The essential oils were hydro distilled from the aerial parts, flowers, leaves, stems, using Clevenger apparatus. The chemical constituents of the essential oils were determined by Gas Chromatography-Mass Spectrometry (GC-MS). As the hydro distillation yielded no oil from the stem, we present only the results for leaves and flowers. The results revealed that the essential oil content varied with a range of 0.29% to 0.33% for flowers and 0.32% to 0.48% for leaves. As usual, the content of essential oils was higher in leaves than in flowers. The Silate-Egyptian accession had the lowest essential oil content obtained from leaves and flowers. However, South Darfur accession from Sudan had the highest oil content but did not significantly differ from Kennana accession. Several oil components were identified based upon comparison of their mass spectral data with those of reference compounds published in literature or stored in a computer library. The combined GC-MS * Corresponding author. Y. A. A. Aburigal et al. 184 analysis showed that methyl eugenol was the major constituent of the essential oil of Kennana accession. While the major constituent of essential oil of South-Darfur and Silate-Egyptian accessions were germacrene and linalool, respectively.
Basil (Ocimum L.) species are used as medicinal plants due to their essential oils exhibiting specific biological activity. The present work demonstrated that both the variety and season/conditions of cultivation had a significant effect on (i) the produced amount (extraction yield), (ii) qualitative, as well as (iii) quantitative profile of basil essential oil. Among studied basil varieties, a new variety, 'Mánes', was characterized for the first time. Based on our quantitative evaluation of GC-MS profiles, the following chemotypes and average concentrations of a main component were detected in the studied basil varieties: 'Ohře', 'Lettuce Leaf', 'Purple Opaal', 'Dark Green' (linalool, 5.99, 2.49, 2.34, 2.01 mg/mL, respectively), and 'Mammolo Genovese', 'Mánes', 'Red Rubin' (eucalyptol, 1.34, 0.96, 0.76 mg/mL, respectively). At the same time, when considering other compounds identified in GC-MS profiles, all the studied varieties, except from 'Lettuce Leaf', were methyl eugenol-rich with a strong dependence of the eugenol:methyl eugenol ratio on the seasonal changes (mainly solar irradiation, but also temperature and relative humidity). More complex and/or variable (depending on the season and cultivation) chemotypes were observed with 'Lettuce Leaf' (plus estragole, 2.27 mg/mL), 'Dark Green' (plus eucalyptol, 1.36 mg/mL), 'Mammolo Genovese' (plus eugenol, 1.19 mg/mL), 'Red Rubin' (plus linalool and eugenol, 0.46 and 0.56 mg/mL, respectively), and 'Mánes' (plus linalool and eugenol, 0.58 and 0.40 mg/mL, respectively). When considering superior extraction yield (ca. 17 mL·kg −1 , i.e., two to five times higher than other examined varieties) and consistent amounts (yields) of essential oil when comparing inter-seasonal or inter-year data (RSD and inter-year difference in mean yield values <2.5%), this new basil variety is very promising for use in the pharmaceutical, food, and cosmetic industries. http://indoessentialoil.com
COMPARATIVE STUDY OF ESSENTIAL OILS OBTAINED FROM TWO BASIL TAXA
Rev. Scientific Papers. Series A. Agronomy, 2022
The objective of this study was to evaluate the essential oils (EOs) obtained from two medicinal plants belonging to the genus Ocimum sp. These were compared in terms extraction yield and analysis of EOs compounds using GC/MS (content, chemical composition and quantitative dosage of some compounds: linalool, estragole and eugenol). Ocimum basilicum L., fam. Lamiaceae with 2 cultivars: Yellow basil (Ocimum basilicum L., 'Aromat de Buzău' variety), Purple basil (Ocimum basilicum cv. purpurascens, 'Seraphim' variety) and Holy basil (Tulsi) was used in the experiments.
Evaluation of Essential Oil Yield and Chemical Components of Selected Basil Cultivars
Biotechnology & Biotechnological Equipment, 2011
The essential oils yield and the chemical compositions of essential oils of broad and narrow basil varieties were assessed for two years. The essential oil was obtained via hydrodistillation and analyzed using gas chromatography. The essential oil extraction yield varied among basil cultivars. The oil extraction yield for broad leaf basil cultivars, Large Leaf Basil and Genovese, was 1.3% and 2.1% respectively, while narrow leaf basil cultivars, Finissimo Verde a Palla and Larosa Emanuele-Sementi, had oil extraction yields of 1.5% and 1.8%, respectively. Regarding the active substances of basil oil, linalool and eugenol were dependent on the cultivar and year of cultivation. In the broad leaf basil cultivars the concentration of linalool remained constant during the two cultivation years, while eugenol content varied from the first to the second year of cultivation. In narrow leaf basil the content of linalool content fluctuated with cultivation year, while eugenol was the same in both experimental years. The broad leaf basil cultivar had on average for both cultivation years 3.8 mg g-1 linallol and 0.5 mg g-1 eugenol and the narrow leaf basil had 2.8 mg g-1 linallol and 0.7 mg g-1 eugenol.
A diallel study of yield components and essential oil constituents in basil (Ocimum basilicum L.)
Industrial Crops and Products, 2012
Introduction: Essential oil of Iraqi growing Ocimum basilicum (Thia basil) was studied for their chemical components investigation and antioxidant activity. Methods: Essential oils of leaves and stems of Ocimum basilicum were collected using hydrodistillation by Clevenger apparatus. Components of the collected essential oils were examined by the techniques such as Gas chromatography and Gas chromatography-mass spectrometry. Results: The analysis showed that the major components of essential oils were linalool (48.69%), 1,8-cineole (14.00%), trans-α-bergamotene (8.23%) and eugenol (6.64%). The antioxidant investigation was achieved using 1,1-diphenyl-2-picrylhydrazyl (DPPH) as a radical scavenger and bleaching of β-carotene/linoleic assay. The essential oil displayed strong inhibition with 110.8% against autoxidation of linoleic acid, while the scavenging of DPPH radical gave a value of IC 50 145.35 µg/mL. Conclusion: The results signify that essential oils of O. Basilicum could be used for pharmaceutical studies and preservative in the food industry. This is the first study of the essential components of new cultivate of Thai basil in Iraq.