Variability in Content and Chemical Constituents of Essential Oil of Sweet Basil (Ocimum basilicum L.) Obtained from Aerial Plant Parts (original) (raw)
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Advances in Bioscience and Biotechnology, 2016
Sweet basil (Ocimum basilicum L.) project that is being conducted at the National Oilseed Processing Research Institute (NOPRI) aims at exploiting the genetic diversity and associated phenotypic variation in the investigated basil germplasm and evaluating key factors such as variety, plant ontogeny and plant parts that affect yield, chemical constituents, aroma and flavor of essential oils. This paper discussed the variability inessential oils content and chemical constituents of the aerial plant parts of the basil. An open-air pot experiment was carried out using three accessions provided by Agricultural Research Corporation-Genetic Resources Unit, Sudan. The essential oils were hydro distilled from the aerial parts, flowers, leaves, stems, using Clevenger apparatus. The chemical constituents of the essential oils were determined by Gas Chromatography-Mass Spectrometry (GC-MS). As the hydro distillation yielded no oil from the stem, we present only the results for leaves and flowers. The results revealed that the essential oil content varied with a range of 0.29% to 0.33% for flowers and 0.32% to 0.48% for leaves. As usual, the content of essential oils was higher in leaves than in flowers. The Silate-Egyptian accession had the lowest essential oil content obtained from leaves and flowers. However, South Darfur accession from Sudan had the highest oil content but did not significantly differ from Kennana accession. Several oil components were identified based upon comparison of their mass spectral data with those of reference compounds published in literature or stored in a computer library. The combined GC-MS * Corresponding author. Y. A. A. Aburigal et al. 184 analysis showed that methyl eugenol was the major constituent of the essential oil of Kennana accession. While the major constituent of essential oil of South-Darfur and Silate-Egyptian accessions were germacrene and linalool, respectively.
Chemical Variability and Chemotype Concept of Essential Oils from Algerian Wild Plants
Molecules
The chemical compositions of eleven wild species of aromatic and medicinal plants indigenous to Algeria, including Thymus, Mentha, Rosmarinus, Lavandula, and Eucalyptus, were analyzed. The identification of the chemical composition of each oil was conducted using GC-FID and GC-MS capillary gas chromatography. The study investigated the chemical variability of the essential oils based on several parameters. These included the impact of the vegetative cycle on oil composition, variations among subspecies of the same species, variations among species within the same genus, the influence of environmental factors on composition variations within a species, chemo typing, and the genetic factors (such as hybridization) contributing to chemical variability. The concepts of chemotaxonomy, chemotype, and chemical markers were examined to understand their limitations and emphasize the importance of regulating the use of essential oils derived from wild plants. The study advocates for an approa...
Physical and chemical analysis of some imported essential oils in the Sudanese market
African Journal of Pharmacy and Pharmacology, 2012
A market survey in Sudan indicated the growing trend of import of essential oils as crude raw materials or in a form of semi-finished or finished products and commodities. Quality control and assessment of the imported essential oils need to be promoted bearing in mind the research and development in this field. The main objectives of the present paper were to evaluate some of these imported essential oils namely: mint oil, clove oil and olibanum oil and to compare their physical and chemical properties with essential oils prepared from plants available in the local market. The essential oils were investigated according to international standard methods: hydrodistillation, thin layer chromatography, gas chromatography-mass spectroscopy and British Pharmacopoeia procedures. According to the results obtained, it was obvious that the physico-chemical properties and chemical composition of traded (imported) essential oils were different when compared with essential oils prepared from local plant samples and were not compatible with the international standards. Quality control and assessment of imported and locally produced essential oils should be promoted with regard to their uses in cosmetics, pharmaceuticals, aromatherapy and food industries.
Essential Oils and Chemical Diversity of Southeast European Populations of Salvia officinalis L
Chemistry & Biodiversity, 2015
The essential oils of 25 populations of Dalmatian sage (Salvia officinalis L.) from nine Balkan countries, including 17 indigenous populations (representing almost the entire native distribution area) and eight non-indigenous (cultivated or naturalized) populations were analyzed. Their essential-oil yield ranged from 0.25 to 3.48%. Within the total of 80 detected compounds, ten (β-pinene, 1,8-cineole, cis-thujone, trans-thujone, camphor, borneol, trans-caryophyllene, α-humulene, viridiflorol, and manool) represented 42.60 to 85.70% of the components in the analyzed essential oils. Strong positive correlations were observed between the contents of trans-caryophyllene and α-humulene, α-humulene and viridiflorol, and viridiflorol and manool. Principal component analysis (PCA) on the basis of the contents of the ten main compounds showed that four principal components had an eigenvalue greater than 1 and explained 79.87% of the total variation. Performing cluster analysis (CA), the sage populations could be grouped into four distinct chemotypes (A-D). The essential oils of 14 out of the 25 populations of Dalmatian sage belonged to Chemotype A and were rich in cis-thujone and camphor, with low contents of trans-thujone. The correlation between the essential-oil composition and geographic variables of the indigenous populations was not significant; hence, the similarities in the essential-oil profile among populations could not be explained by the physical proximity of the populations. Additionally, the southeastern populations tended to have higher EO yields than the northwestern ones.
Essential Oil from Ocimum basilicum (Omani Basil): A Desert Crop
Natural Product Communications, 2011
The focus of the present study was on the influence of season on yield, chemical composition, antioxidant and antifungal activities of Omani basil ( Ocimum basilicum) oil. The present study involved only one of the eight Omani basil varieties. The hydro-distilled essential oil yields were computed to be 0.1%, 0.3% and 0.1% in the winter, spring and summer seasons, respectively. The major components identified were L- linalool (26.5 - 56.3%), geraniol (12.1 - 16.5%), 1,8-cineole (2.5 - 15.1%), ρ-allylanisole (0.2 - 13.8%) and DL-limonene (0.2 -10.4%). A noteworthy extra component was β- farnesene, which was exclusively detected in the oil extracted during winter and spring at 6.3% and 5.8%, respectively. The essential oil composition over the different seasons was quite idiosyncratic, in which the principal components of one season were either trivial or totally absent in another. The essential oil extracted in spring exhibited the highest antioxidant activity (except DPPH scavenging...
Journal of Essential Oil Bearing Plants
Lippia citriodora H.B.K is an herbal species which contains several aroma compounds and the leaves of the plant are utilized as a seasoning for food preparations and flavoring beverages. In this study, the essential oil composition of the L. citriodora growing in semi-arid ecological conditions in Diyarbakır Turkey was determined at different plant parts taken from the upper (leaves and flowers), middle (leaves), and lower portions (leaves). The highest essential oil content (3.7 µl/g in w/w) was obtained from the lower part of the plant. The essential oil of L. citriodora was analyzed by GC/MS and GC/FID. Although the major essential oil composition of the oil was obtained as caryophyllene oxide, limonene, geranial, and neral, caryophyllene oxide and limonene showed significant changes with respect to different plant parts. While the rate of caryophyllene oxide gradually decreases from the lower parts of the plant to the upper parts; it was observed that the limonene ratio increases towards the upper parts of the plant. Principal component analysis (PCA) on the basis of the contents of the 31 compounds showed that two principal components had an eigen value greater than 1 and the first principal component explained 80. 39% of the total variation. In addition to the remarkable chemical differences of L. citriodora essential oil regarding the caryophyllene oxide and limonene, the proportion of these compounds has been observed to be significantly higher than the literature values.
Molecules
Ocimum campechianum Mill. (Peruvian basil) is an essential oil-bearing plant of the Lamiaceae family. Volatile oil produced through steam distillation of Peruvian basil was examined to establish the aromatic and stable isotope profiles of samples (n = 9) from three different cultivated plots in Peru. The resulting essential oils were analyzed by GC/FID, GC/MS, and GC/IRMS. In accordance with findings from other researchers, multiple chemotypes, defined by the most abundant aromatic compounds, exist within these populations. Overall, 55% of samples are the eugenol chemotype (values ranging 15.4–30.2%), 33% are the methyl eugenol chemotype (values ranging 68.1–68.7%), and a single sample is a mixture of both chemotypes, containing high levels of both eugenol (38.1%) and methyl eugenol (8.6%). Stable isotope ratios, δ2H and δ13C, performed on prominent compounds provide supporting data for distinguishing chemotypes. Complete aromatic profiles, stable isotope ratios, and essential oil y...
Essential-Oil Variability in a Collection ofOcimum basilicumL. (Basil) Cultivars
Chemistry & Biodiversity, 2016
Ocimum basilicum L. (Lamiaceae) is an aromatic plant of great tradition in the Mediterranean area. Its economic importance is growing up determining an expansion of cultivation. This paper evaluated the morphological traits, the chemical profiles, and antibacterial activity of 21 cultivars of basil belonging to ʻGenoveseʼ, ʻNapoletanoʼ, and ʻPurple basilʼ types. The cultivars were characterized by different growth rate and morphological traits. The chemical composition of the oils analyzed by GC and GC/MS analysis, supported by the PCA analysis, underlined the strong influence of chemotype. It is noteworthy that estragole, never present in Genovese and purple basil types, occurred in Napoletano type. The high presence of eugenol, methyl eugenol, and linalool in the majority of cultivars, belonging both to Genovese and to Napoletano types was registered. Of great interest resulted the composition of the purple basil ʻOpalʼ. All the samples tested exhibited similar antibiotic profiles with moderate antibacterial activity. The results enhanced the importance of determination of essential-oil profile in the selection of cultivars characterized by diverse morphological traits and are useful for different purposes.
Variability in essential oil composition of Turkish basils (Ocimum basilicum L.)
Sweet basil (Ocimum basilicum L.), one of the most popular aromatic plants, shows great variation in both morphology and essential oil components. In this study, the composition of 18 Turkish basil essential oils was investigated by GC and GCeMS. Variation of essential oils in the landraces was subjected to cluster analysis, and seven different chemotypes were identified. They were (1) linalool, (2) methyl cinnamate, (3) methyl cinnamate/linalool, (4) methyl eugenol, (5) citral, (6) methyl chavicol (estragol), and (7) methyl chavicol/citral. Methyl chavicol with high citral contents (methyl chavicol/citral) can be considered as a ‘‘new chemotype’’ in the Turkish basils. Because methyl eugenol and methyl chavicol have structural resemblance to carcinogenic phenylpropanoids, chemotypes having high linalool, methyl cinnamate or citral contents and a mixture of these is suitable to cultivate for use in industry.
GC-MS Analysis of the Essential Oil Obtained From Ocimum Basilicum L. “Holland” Cultivar
Farmacia
This paper pres ents the quantitative and qualitative analysis of the essential oils obtained from a “Holland” cultivar of Ocimum basilicum L (basil). The vegetal material was harvested from the experimental fields of the University of Agricultural Sciences and Veterinary Medicine Cluj - Napoca (Romania) at different times of the year: June (sample 1), August (sample 2) and September (sample 3), 2008. The essential oils obtained by steam distillation were analyzed by gas - chromatography coupled with mass spectrometry (GC - MS). The content in essential oils ranged from 0.4% to 0.8%; the maximum amounts were observed in June while the minimum in September. The most important components identified in essential oils were: linalool, α - cadinol, trans - α - bergamotene, γ - cadinene, β - elemene, methylchavicol and delta - guaiene . We can consider that the „Holland” cultivar of Ocimum basilicum may be considered as „linalool - rich” chemotype [10], because for all essential oil sample...