MO015ANALYZING the Effect of Microplastic Particles on Human Podocytes (original) (raw)
Related papers
Acute and Sub-Chronic Effects of Microplastics (3 and 10 µm) on the Human Intestinal Cells HT-29
International Journal of Environmental Research and Public Health, 2021
Due to ingestion of contaminated foods, the human gastrointestinal tract is the most likely site of exposure to microplastics (MPs) with gut barrier dysfunction and intestinal inflammation. Aimed to assess the effects induced by MPs with different granulometry (polystyrene (PS) 3 and 10 µm), we performed an in vitro study by using the human intestinal cell line HT29. As a novelty, we assessed the sub-chronic exposure extending the treatment up to 48 days simulating the in vivo situation. In the range of 100–1600 particles mL−1, both the PS suspensions had moderate cytotoxicity after 24 h with percentages of mortality between 6.7 and 21.6 for the 10 µm and 6.1 and 29.6 for the 3 µm PS. Microscopic observation highlighted a more pronounced lysosomal membrane permeabilization in HT29 exposed to PS 3µm. Reactive oxygen species production was higher in cells exposed to PS 10 µm, but sub-chronic exposure highlighted the ability of the cells to partially neutralize this effect. Comet-assay...
#6111 First Identification and Characterization of Microplastics in Human Kidney and Urine
Nephrology Dialysis Transplantation
Background and Aims Microplastics (MPs), plastic fragments less than 5mm in diameter, have been recognized as a global environmental issue. Various studies have shown their ubiquitous presence and their toxicity on marine and terrestrial fauna. Currently in humans, the presence of MP has been evidenced in lungs, placenta, blood, and liver. However, there is still very little knowledge about their presence in different organs and tissues and their prospective toxicity. The objective of this study was to investigate the presence of microplastics in human kidneys and urine. Method We obtained 10 urine samples from healthy individuals and 10 kidney healthy tissue samples from nephrectomies in renal cancer The detection and characterization of microplastics was performed by a light microscope (Leica, total magnification of 500x) coupled to a Raman spectrometer (Renishaw System 2000). To this purpose, specimens were digested by a 10% KOH solution at 60°C and subsequently filtered on membr...
The Plastic Within: Microplastics Invading Human Organs and Bodily Fluids Systems
Environments
Microplastics (MPs), small plastic particles resulting from the degradation of larger plastic items and from primary sources such as textiles, engineered plastic pellets, etc., have become a ubiquitous environmental pollutant. As their prevalence in the natural environment grows, concerns about their potential impacts on human health have escalated. This review discusses current research findings on the presence of MPs in organs such as the liver, blood, heart, placenta, breast milk, sputum, semen, testis, and urine, while also exploring plausible mechanisms of translocation. Furthermore, the review emphasizes the importance of understanding the potential toxicological effects of MPs on various physiological processes within these organs and their broader implications for human health. This review also examines the pathways through which MPs can enter and accumulate in human organs and bodily fluids, shedding light on the intricate routes of exposure and potential health implication...
Biomolecules
Plastic is a polymer extremely resistant to degradation that can remain for up to hundreds or thousands of years, leading to the accumulation of massive amounts of plastic waste throughout the planet’s ecosystems. Due to exposure to various environmental factors, plastic breaks down into smaller particles named microplastics (1–5000 μm) and nanoplastics (<1 μm). Microplastics (MPs) are ubiquitous pollutants but, still, little is known about their effects on human and animal health. Herein, our aim is to investigate cytotoxicity, oxidative stress, inflammation and correlated gene modulation following exposure to polystyrene microplastics (PS-MPs) in HRT-18 and CMT-93 epithelial cell lines. After 6, 24 and 48 h PS-MPs treatment, cell viability (MTT) and oxidative stress (SOD) assays were performed; subsequently, expression changes and cytokines release were investigated by Real-Time PCR and Magnetic-beads panel Multiplex Assay, respectively. For each exposure time, a significantly ...
PLASTICS AND THEIR ADDITIVES REACHED THE BLOOD AND TISSUE SPACES: WHAT ARE THE POSSIBLE CONSEQUENCES
Exploratory Animal and Medical Research, 2022
Microplastics, the tiny plastic particles are either manufactured for definite purposes or develop as the fragmented part of larger plastic materials. These can reach everywhere on the surface of our planet to do various detrimental impacts on humans and animals living on the land and in the sea, directly or after entering the food chain; on the overall biosphere as well as on the total environment by staying at a non-biodegradable state for centuries. The study of various health effects of microplastics is a comparatively new subject and different aspects of serious to very serious impacts of microplastics and their additives on health are becoming clear by such research. It is known that microplastics have already reached the tissues and become a reason for the death of fish and other edible or non-edible lives in the sea and water bodies. Possible effects of microplastics and their additive chemicals can be assumed from some experimental studies and the logical correlation of reports of such studies with the direct observations of the health status of living human beings. Recently it has been reported that microplastics and their additives already reached human blood and the placenta. Possible impacts of these chemicals on the endocrine system, their role in initiation and progression of different chronic diseases including cancers, effect on the brain, overall nervous system, and related behavioral changes as well as on the important organs of the body, like the liver, etc. are discussed in the light of published literature with an effort to search out the possible ways to reduce the load of microplastics and their additives inside our body to combat the incoming massacre.