Mussel Inspired Chemistry and Bacteria Derived Polymers for Oral Mucosal Adhesion and Drug Delivery (original) (raw)
Related papers
Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials
Marine Drugs, 2015
The substance secreted by mussels, also known as nature's glue, is a type of liquid protein that hardens rapidly into a solid water-resistant adhesive material. While in seawater or saline conditions, mussels can adhere to all types of surfaces, sustaining its bonds via mussel adhesive proteins (MAPs), a group of proteins containing 3,4-dihydroxyphenylalanine (DOPA) and catecholic amino acid. Several aspects of this adhesion process have inspired the development of various types of synthetic materials for biomedical applications. Further, there is an urgent need to utilize biologically inspired strategies to develop new biocompatible materials for medical applications. Consequently, many researchers have recently reported bio-inspired techniques and materials that show results similar to or better than those shown by MAPs for a range of medical applications. However, the susceptibility to oxidation of 3,4-dihydroxyphenylalanine poses major challenges with regard to the practical translation of mussel adhesion. In this review, various OPEN ACCESS Mar. Drugs 2015, 13 6793 strategies are discussed to provide an option for DOPA/metal ion chelation and to compensate for the limitations imposed by facile 3,4-dihydroxyphenylalanine autoxidation. We discuss the anti-proliferative, anti-inflammatory, anti-microbial activity, and adhesive behaviors of mussel bio-products and mussel-inspired materials (MIMs) that make them attractive for synthetic adaptation. The development of biologically inspired adhesive interfaces, bioactive mussel products, MIMs, and arising areas of research leading to biomedical applications are considered in this review.
Mussel-inspired Multifunctional Polymeric Materials with Bioengineering Applications
2017
Mussels can obtain strong underwater attachment to virtually all kinds of surfaces including rocks, metals, wood structures, polymers and concretes by secreting mussel foot proteins to form byssus. Great efforts have been dedicated to understanding this behavior and it is found that an catecholic amino acid 3,4dihydroxyphenyl-L-alanine (DOPA) plays a crucial role in achieving this remarkable adhesion performance by actively involved in various catecholmediated interactions such as covalent bonding, hydrogen bonding, metal coordination, cation-π interaction and aromatic interaction. Recently the selfhealing capability of myssel byssal threads has attracted great attention and it is found that catechol-mediated reversible interactions such as catechol-metal In the third project, an injetable self-healing hydrogel with antimicrobial and antifouling properties was prepared. An ABA tri-block copolymer employing catechol functionalized PEG as the thermo-sensitive A block and poly{[2-(methacryloyloxy)-ethyl] trimethyl ammonium iodide}(PMETA) as the hydrophilic and antimicrobial B block was synthesized. The hydrogel prepared through selfassembly of this triblock copolymer shows excellent sol-gel thermo-reversibility, can effectively inhibit the growth of E. coli (>99.8% reduction in bacterial counts) and prevent nonspecific cell attachment. What's more, it can heal autonomously from repeated damage, through mussel-inspired catechol-mediated hydrogen bonding and aromatic interactions, exhibiting great potential in various bioengineering applications. v Preface This thesis is an original work by Lin Li (L. Li) under the supervision from Prof. Hongbo Zeng (H. Zeng). Chapter 2 of this thesis has been published as L. Li, B. Yan, L. Zhang, Y. Tian and H. Zeng, Mussel-inspired antifouling coatings bearing polymer loops, Chem Commun 2015, 51, 15780-15783. I was primarily responsible for the data collection and analysis as well as the manuscript composition. B. Yan instructed the polymer synthesis and L. Zhang assisted with the AFM imaging. Y. Tian joined in the research design. H. Zeng was the supervisory author and was involved in the experimental design and manuscript composition. Chapter 3 of this thesis has been published as L.
Frontiers in Chemistry
A simple two-step, shaking-assisted polydopamine (PDA) coating technique was used to impart polypropylene (PP) mesh with antimicrobial properties. In this modified method, a relatively large concentration of dopamine (20 mg ml −1) was first used to create a stable PDA primer layer, while the second step utilized a significantly lower concentration of dopamine (2 mg ml −1) to promote the formation and deposition of large aggregates of PDA nanoparticles. Gentle shaking (70 rpm) was employed to increase the deposition of PDA nanoparticle aggregates and the formation of a thicker PDA coating with nano-scaled surface roughness (RMS = 110 nm and Ra = 82 nm). Cyclic voltammetry experiment confirmed that the PDA coating remained redox active, despite extensive oxidative cross-linking. When the PDA-coated mesh was hydrated in phosphate saline buffer (pH 7.4), it was activated to generate 200 µM hydrogen peroxide (H 2 O 2) for over 48 h. The sustained release of low doses of H 2 O 2 was antibacterial against both grampositive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. PDA coating achieved 100% reduction (LRV ∼3.15) when incubated against E. coli and 98.9% reduction (LRV ∼1.97) against S. epi in 24 h.
A Simple Strategy to Achieve Mussel-Inspired Highly Effective Antibacterial Coating
Macromolecular Materials and Engineering
effectively prevent the initial attachment of bacteria to implant and device surfaces and subsequently avoid the device-related infection. [18-21] In order to obtain such antibacterial coatings, several mussel-inspired polymers, including polydopamine, [22,23] catechol-derived poly(ethylene glycol), [24,25] and poly(acrylate-co-acrylamide), [26] were chosen as the coating matrixes. Many ingredients, such as Cu 2+ , [27] silver nanoparticle, [28,29] amphiphilic groups, [30] antibiotic, [31,32] peptide, [33] and natural antibacterial agent [34-36] (i.e., tannin, chitosan, and bronel) have been incorporated into these matrix for antibacterial studies. Although these mentioned coatings are effective in prohibition of bacterial growth on surfaces, sophisticated procedures such as surface-initiated polymerization, layerby-layer deposition, and chemical grafting are generally involved in the fabricating process. [37-40] Thus, to develop more facile and simpler methods for preparing mussel-inspired antibacterial coatings are still challenging and deserve further study. Investigations on the attachment plaques from various mussels reveal that the reaction of catechol and amine group (in lysine residues) via the Michael addition or Schiff base reactions play a critical role in the formation of water-insoluble, 3D marine adhesive proteins (MAP) networks. [5,12,41-45] Inspired by this characteristic, Li et al. developed a water-resistant wood adhesive by simply mixing two commercialized materials, branched polyethyleneimine (PEI), a low-cost and amine groups-enriched industrially material and tannin (TA), a natural product rich in catechol groups. [46] Subsequently, this simple and low-cost technology was also used to fabricate functional coatings. [47-50] For example, Wang et al. fabricated hydrophilic modified polypropylene separators by the co-deposition of catechol and polyamine for Li-ion batteries. [51] Xu et al. reported the co-deposition of catechol and PEI onto nanofiltration membrane for textile wastewater treatment. [52] What's more, based on this technology, the usage of noneconomic catechol-containing materials such as dopamine (including polydopamine) or synthetic catechol-derived polymers was also avoided. Nevertheless, to our best knowledge, this simple and facile method has not been applied in the design of mussel-inspired antibacterial coatings. In this study, we, for the first time, synthesized a musselinspired antibacterial coating via the simple co-deposition of PEI and a quaternized catechol (QCat) in an aqueous Antibacterial Coatings Although significant progress has been made in the preparation of musselinspired antibacterial coatings, continual challenges still remain in pursuing more facile and simpler fabrication methods to construct more robust and effective coatings. In this study, quaternized catechol (QCat), which is synthesized via a simple quaternization reaction from two commercially available materials, 2-chloro-3′,4′-dihydroxyacetophenone and N,N-dimethyldodecylamine, is used as a reactive antimicrobial agent to fabricate musselinspired antibacterial coatings. Specifically, QCat reacts with branched polyethyleneimine (PEI) in Tris-HCl solution through a cross-linking reaction between amino and catechol groups to form a homogeneous coating on various substrates via a simple co-deposition process. The formed PEI/QCat coating exhibits highly effective antimicrobial activity against both Staphylococcus aureus and Escherichia coli and good adhesion on glass, metal, and plastic substrates. Such a simple fabrication process makes it a potential candidate for industrial and medical applications.
Applications of natural polymers in mucoadhesive drug delivery: An overview
Advance pharmaceutical journal, 2018
Aim of this review is to compile the current literature with special focus on role of natural polymers on mucoadhesive drug delivery system. Mucodhesion refers to bond formed between two biological surfaces or a bond connecting a biological and a synthetic polymer surface. Under this drug delivery, buccal mucosa is the preferred site for both systemic and local drug action because the mucosa has a rich blood supply and it relatively permeable. Different bioadhesive dosages form available in market such as Chewing gum, tablets, Patches, Hydrogel, Thiolated tablets. In this review article the Application of natural mucoadhesive polymers advantages, disadvantages and future prospects in Buccal drug delivery has been discussed.
The application of novel mussel-inspired compounds in dentistry
Objective. To give a current review of the mechanism of mussel adhesion, the application of mussel-inspired compounds in dentistry and the challenges associated with clinical application. Methods. Inspired by the wet adhesion property of 3,4-dihydroxyphenol-l-alanine (Dopa) in mussel plaques, various chemical compounds have been synthesized to mimic the mussel as an adhesion model for medical applications. Similar to mussels in the marine environment, dental materials in the oral environment have to endure long-term water hydrolysis, mechanical stress and other chemical challenges. These challenges have influenced an increasing number of studies that are exploring the translation of mussel-inspired adhesion to clinical applications. Therefore, this review discusses the mussel adhesion chemistry and its related application in dentistry. Results. Mussel-inspired compounds have achieved relatively acceptable performances in various dental fields, including surface coating, metal ions chelation, dentin bonding and mucosal adhesion. However, two practical problems remain to be comprehensively addressed, namely the protection of catechol groups from oxidation, and the feasibility for clinical application. Significance. The mussel's wet adhesion ability has attracted much research interest in the dental field because of its properties of moisture-resistant adhesion and surface coating. Despite the emergence of several mussel-inspired compounds in recent years, a comprehensive and timely review of their applications in dentistry is lacking. Therefore, the current review hopes to provide valuable information around the application of mussel-inspired compounds in dentistry with their pros and cons discussed.
A simple two-step, shaking-assisted polydopamine (PDA) coating technique was used to impart polypropylene (PP) mesh with antimicrobial properties. In this modified method, a relatively large concentration of dopamine (20 mg ml −1 ) was first used to create a stable PDA primer layer, while the second step utilized a significantly lower concentration of dopamine (2 mg ml −1 ) to promote the formation and deposition of large aggregates of PDA nanoparticles. Gentle shaking (70 rpm) was employed to increase the deposition of PDA nanoparticle aggregates and the formation of a thicker PDA coating with nano-scaled surface roughness (RMS = 110 nm and Ra = 82 nm). Cyclic voltammetry experiment confirmed that the PDA coating remained redox active, despite extensive oxidative cross-linking. When the PDA-coated mesh was hydrated in phosphate saline buffer (pH 7.4), it was activated to generate 200 µM hydrogen peroxide (H 2 O 2 ) for over 48 h. The sustained release of low doses of H 2 O 2 was antibacterial against both grampositive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria. PDA coating achieved 100% reduction (LRV ∼3.15) when incubated against E. coli and 98.9% reduction (LRV ∼1.97) against S. epi in 24 h.
Bioadhesive Polymers for Controlled Drug Delivery
Annals of the New York Academy of Sciences, 1987
Bioadhesion may be defined' as the state in which two materials, at least one of which is of a biological nature, are held together for an extended period of time by interfacial forces. Thus, attachment of a biological object to another biological object, e.g., cell attachment, or a synthetic polymer to a biological substrate, e.g., denture fixative, are examples of bioadhesion. For drug delivery purposes the term bioadhesion infers attachment of a drug carrier system to a specific biological location. The biological surface can be epithelial tissue or it can be the mucous coat on the surface of the tissue. If adhesive attachment is to the mucin coat the phenomena is referred to as mucoadhesion. Bioadhesion can be modeled after bacterial attachment to tissue surfaces and mucoadhesion can be modeled after much organization on epithelial tissue.