Formulation and evaluation of Hydrogel for wound healing (original) (raw)
Related papers
International Journal of Pharmaceutics, 2013
Keeping in view the antioxidant nature of the acacia gum and mucoadhesive nature of carbopol hydrogels, in the present studies, an attempt has been made to explore the potential of these materials in designing new hydrogel wound dressings meant for slow release of gentamicin, an antibiotic drug, and to enhance the wound healing potential. The hydrogel films were characterized by SEM, FTIR, XRD and swelling studies. Biomedical properties of hydrogel films like blood compatibility, antioxidant activity, mucoadhesion, antimicrobial activity, oxygen/water vapour permeability, microbial penetration and mechanical properties (tensile strength, burst strength, resilience, relaxation, and folding endurance) have been evaluated. The histological studies of wound healing were also carried out on swiss albino mice of strain Balb C and it has been observed that in case of wounds covered with hydrogel dressings shown faster wound healing, formation of well developed fibroblasts and blood capillaries as compared to open wounds. The results of biomedical properties indicated that hydrogel films are non-thrombogenic, non-haemolytic, antioxidant and mucoadhesive in nature, and are permeable to oxygen and moisture while impermeable to microorganisms. The hydrogel wound dressings have absorbed (8.772 ± 0.184 g/g film) simulated wound fluid. Release of gentamicin drug from wound dressings occurred through Fickian diffusion mechanism in simulated wound fluid.
Polymers
Wound healing faces significant challenges in clinical settings. It often contains a series of dynamic and complex physiological healing processes. Instead of creams, ointments and solutions, alternative treatment approaches are needed. The main objective of the study was to formulate bacitracin zinc-loaded topical patches as a new therapeutic agent for potential wound healing. A free radical polymerization technique was optimized for synthesis. Polyethylene glycol-8000 (PEG-8000) was chemically cross-linked with acrylic acid in aqueous medium, using Carbopol 934 as a permeation enhancer and tween 80 as surfactant. Ammonium persulfate and N,N’-Methylenebisacrylamide (MBA) were utilized as initiator and cross-linker. FTIR, DSC, TGA, and SEM were performed, and patches were evaluated for swelling dynamics, sol-gel analysis, in vitro drug release in various media. A Franz diffusion cell was used for the permeation study. Irritation and wound healing with the drug-loaded patches were al...
Gels
Drug administration to the wound site is a potential method for wound healing. The drug retention duration should be extended, and drug permeability through the buccal mucosal layer should be regulated. Oral wounds can be caused by inflammation, ulcers, trauma, or pathological lesions; if these wounds are not treated properly, they can lead to pain, infection, and subsequent undesirable scarring. This study aimed to develop Kolliphor-407 P-based gel containing neomycin sulfate (NES) loaded in solid lipid nanoparticles (SLNs) and enhance the antimicrobial activity. By considering lipid concentrations and achieving the lowest particle size (Y1) and maximum entrapment (EE-Y2) effectiveness, the formulation of NES-SLN was optimized using the Box–Behnken design. For the selected responses, 17 runs were formulated (as anticipated by the Design-Expert software) and evaluated accordingly. The optimized formulation could achieve a particle size of 196.25 and EE of 89.27% and was further util...
Development and Characterization of Novel Medicated Hydrogels for Wound Dressing
Soft Materials, 2010
The medicated hydrogels were prepared aseptically under moist heat treatment using polyvinylpyrrolidone (PVP), sodium-carboxymethylcellulose (CMC), polyethyleneglycol (PEG), agar, glycerin and/or boric acid (BA) and designated as PVP-CMC and PVP-CMC-BA. The aim of this study was to develop some medicinal values-based hydrogels. BA was used to build up the medicinal values (antiseptic and antimicrobial properties) within the hydrogels. Optical images, scanning electron microscopy (SEM), and Fourier transform infrared (FTIR) spectroscopy of the hydrogels indicated that boric acid is uniformly dispersed within the cross-linked hydrogel network that may heal and protect the wounds from infections/sepsis. Swelling study in presence of water and physiological saline solution confirmed its reasonable absorption capacity. The rheological properties and mechanical properties demonstrated that the boric acid incorporated hydrogels are quite flexible. Assessment of antimicrobial property study proves that PVP-CMC-BA (3 % BA) has strong infection protection capacity.
Characterization of Neomycin-Loaded Xanthan-Chitosan Hydrogels for Topical Applications
Cellulose Chemistry and Technology, 2019
Xanthan-chitosan hydrogels are recommended for encapsulation and controlled release of therapeutic agents. Chitosan and neomycin sulphate have been shown to have wound healing properties, when used individually, and a synergetic effect when used together. In this study, drug-loaded xanthan-chitosan hydrogels were prepared with two different neomycin sulphate concentrations, as potential topical formulations. These hydrogels were characterized by rheological studies, thermal analyses, for in vitro drug release diffusion, cytotoxicity and antibacterial activity. The results obtained from dynamic rheological experiments demonstrated that the hydrogels behave as weak gels and that their characteristics are strongly influenced by the concentration, pH and temperature. The results provided by thermal analysis suggested that an interaction occurs between xanthan-chitosan and neomycin sulphate. In vitro studies demonstrated good retardation of drug release from the hydrogels. Moreover, cell viability studies showed that the hydrogels are non-cytotoxic. Furthermore, the prepared formulations revealed effective bacterial inhibition when exposed to cultures of Escherichia coli and Staphylococcus aureus.
Permeability and Biocompatibility of Novel Medicated Hydrogel Wound Dressings
Soft Materials, 2010
Hydrogel dressings are being popularized for wound care management because of their softness, tissue compatibility, and ability to enhance wound healing process. PVP-CMC and PVP-CMC-BA hydrogels were prepared using polyvinylpyrrolidone (PVP), sodium-carboxymethylcellulose (CMC), polyethyleneglycol (PEG), agar, glycerine and without/with boric acid (BA). Permeability: water vapor transmission and microbe penetration and biocompatibility: cytotoxicity, skin irritation, and skin sensitization tests of hydrogels were performed. Water vapor transmission ability of hydrogels shows positive response to gas permeability, whereas microbe penetration ability by Staphylococcus aureus and Escherichia coli shoius negative penetrability; thus these two hydrogel dressings could be considered as a good barrier against microbes having an excellent oxygen diffusion property. In vitro assay of cytotoxicity tuas carried out in presence of human immortalized non-tumorigenic keratinocyte cell line (HaCaT), which was further confirmed with balb/c 3T3 mouse fibroblasts cells. The skin irritation test was evaluated on albino rabbits and sensitization test was carried out on albino guineapigs under in vivo stale. Both PVP-CMC and PVP-CMC-BA represented mostly negative effect on cytotoxicity, dermal irritation, and/ or sensitization. All the results indicate that both PVP-CMC and PVP-CMC-BA hydrogels have great potential for biomedical applications, especially for burn skin treatment as well as wound dressings materials.
A Technical Overview: Polymers for Wounds and Burns Dressing as Hydrogel Drug Delivery System
International Journal of Pharmaceutical Sciences Review and Research
In the last years, health care professionals faced with an increasing number of patients suffering from wounds and burns difficult to treat and heal. During the wound healing process, the dressing protects the injury and contributes to the recovery of dermal and epidermal tissues. Because their biocompatibility, biodegradability and similarity to macromolecules recognized by the human body, some natural polymers such as polysaccharides (alginates, chitin, chitosan, heparin, chondroitin), proteoglycans and proteins (collagen, gelatin, fibrin, keratin, silk fibroin, eggshell membrane) are extensively used in wounds and burns management. Obtained by electrospinning technique, some synthetic polymers like biomimetic extracellular matrix micro/nanoscale fibres based on polyglycolic acid, polylactic acid, polyacrylic acid, poly-ε-caprolactone, polyvinylpyrrolidone, polyvinyl alcohol, polyethylene glycol, exhibit in vivo and in vitro wound healing properties and enhance re-epithelializatio...
Journal of Pharmaceutical and Biopharmaceutical Research, 2019
In the present work an attempt has been made to design the antibiotic drug loaded carbopolpoly(NVP) based hydrogel wound dressings for better wound care. The polymer films were characterized by SEM-EDX, AFM, FTIR, 13 CNMR, TGA/DTA/DTG, DSC, and swelling studies. Besides drug release, various biomedical properties (viz. blood compatibility, mucoadhesion, oxygen permeability, water vapour transmission rate, microbial penetration, tensile strength, bursting strength, resilience, stress relaxation, and folding endurance) have also been studied. The polymer films have been observed to be biocompatible, permeable to oxygen and water vapour and have absorbed simulated wound fluid 11.37±0.31g/g of polymer film. The drug release profile followed the Case-II diffusion mechanism and release profile best fitted in Hixson-Crowell's kinetic models. Mechanical properties results showed that the polymer film had 0.65±0.12 Nmm −2 tensile strength, 119.38±14.26% elongationand 25.49±0.72% resilience.
Advanced Hydrogels as Wound Dressings
Biomolecules
Skin is the largest organ of the human body, protecting it against the external environment. Despite high self-regeneration potential, severe skin defects will not heal spontaneously and need to be covered by skin substitutes. Tremendous progress has been made in the field of skin tissue engineering, in recent years, to develop new skin substitutes. Among them, hydrogels are one of the candidates with most potential to mimic the native skin microenvironment, due to their porous and hydrated molecular structure. They can be applied as a permanent or temporary dressing for different wounds to support the regeneration and healing of the injured epidermis, dermis, or both. Based on the material used for their fabrication, hydrogels can be subdivided into two main groups—natural and synthetic. Moreover, hydrogels can be reinforced by incorporating nanoparticles to obtain “in situ” hybrid hydrogels, showing superior properties and tailored functionality. In addition, different sensors can...
Effect of Various Formulation Parameters on the Properties of Hydrogel Wound Dressings
Drug Delivery Letters, 2012
The work presented in this manuscript was undertaken to evaluate the effect of various formulation parameters on the essential properties of hydrogel wound dressings. PVA and PVP based hydrogel dressing systems were prepared by irradiation cross-linking and freeze thawing method. Prepared hydrogels were characterized for swelling index, water vapor transmission rate (WVTR), mechanical strength, gel fraction and in-vitro drug release. Hydrogel dressings prepared by irradiation method showed better physico-mechanical properties like swelling index, WVTR, and transparency as compared to hydrogels prepared by freeze thaw method, except the mechanical strength, which was less in case of hydrogels, prepared using irradiation method. An increase in concentration of PVP resulted in increase in tensile strength and swelling index, and decrease in gel fraction of hydrogel due to higher degree of cross linking. Overall, addition of PVP improves physico-mechanical properties of hydrogel as compared to Plain PVA containing hydrogels. An increase in irradiation dose increases tensile strength, but decreases equilibrium swelling of hydrogels. Gel fraction also increases with increase in irradiation dose up of 50 to 60 kGy, however further increase in irradiation dose decreases gel fraction of hydrogel, as polymer degradation process exceeds cross linking process. Finally, the drug release studies were performed using silver sulfadiazine (AgSD) as a model drug in hydrogel prepared by freeze-thaw method and drug release was found to be zero-order suggesting constant drug availability on the hydrogel applied wound, which is highly desirable.