Using synthetic biology to overcome barriers to stable expression of nitrogenase in eukaryotic organelles (original) (raw)

An experimental workflow identifies nitrogenase proteins ready for expression in plant mitochondria

Keren Byrne

2019

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Expression of 16 Nitrogenase Proteins within the Plant Mitochondrial Matrix

Andrew Warden

Frontiers in plant science, 2017

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Plant expression of NifD protein variants resistant to mitochondrial degradation

Keren Byrne

Proceedings of the National Academy of Sciences, 2020

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Purification and In Vitro Activity of Mitochondria Targeted Nitrogenase Cofactor Maturase NifB

Carlos Erasun

Frontiers in Plant Science

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Exploiting genetic diversity and gene synthesis to identify superior nitrogenase NifH protein variants to engineer N2-fixation in plants

Stefan Burén

Communications Biology, 2021

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Expression of Active Subunit of Nitrogenase via Integration into Plant Organelle Genome

Jeffrey Staub

PloS one, 2016

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A Synthetic Biology Workflow Reveals Variation in Processing and Solubility of Nitrogenase Proteins Targeted to Plant Mitochondria, and Differing Tolerance of Targeting Sequences in a Bacterial Nitrogenase Assay

Keren Byrne

Frontiers in Plant Science, 2020

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Engineering a functional NifDK polyprotein resistant to mitochondrial degradation

Andrew Warden

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Use of synthetic biology tools to optimize the production of active nitrogenase Fe protein in chloroplasts of tobacco leaf cells

Stefan Burén

Plant Biotechnology Journal, 2020

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State of the art in eukaryotic nitrogenase engineering

Stefan Burén

FEMS microbiology letters, 2018

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Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae

Stefan Burén

ACS Synthetic Biology, 2017

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Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast

jose hernandez

Nature communications, 2016

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Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae

Stefan Burén

ACS synthetic biology, 2017

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Functional Nitrogenase Cofactor Maturase NifB in Mitochondria and Chloroplasts of Nicotiana benthamiana

Emma Barahona

mBio

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Using Synthetic Biology to Distinguish and Overcome Regulatory and Functional Barriers Related to Nitrogen Fixation

Yiping Wang

PLoS ONE, 2013

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Functional expression of the nitrogenase Fe protein in transgenic rice

Stefan Burén

Communications Biology

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Using synthetic biology to increase nitrogenase activity

XIN LI

Microbial cell factories, 2016

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Studies of the Potential for Expression of Nitrogenase Fe-Protein in Cells of Higher Plants

amanda dowson

Nitrogen Fixation, 1991

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A colorimetric method to measure in vitro nitrogenase functionality for engineering nitrogen fixation

Stefan Burén

Scientific Reports

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Exploring the Intrinsic Limits of Nitrogenase Transfer from Bacteria to Eukaryotes

Gabriela Soto

Journal of Molecular Evolution, 2013

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Sequential and differential interaction of assembly factors during nitrogenase MoFe protein maturation

Carlos Erasun

The Journal of biological chemistry, 2018

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Analysis of Nitrogenase Fe Protein Activity in Transplastomic Tobacco

Stefan Burén

Frontiers in Agronomy, 2021

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Crystallography fixes it for nitrogenase

Robert Eady

Current Biology, 1992

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Nif gene transfer and expression in chloroplasts: Prospects and problems

amanda dowson

Springer eBooks, 1997

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Expression of a nitrogen-fixation gene encoding a nitrogenase subunit in yeast

Judith Berman

Gene, 1985

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Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co in Saccharomyces cerevisiae

Stefan Burén

Proceedings of the National Academy of Sciences, 2019

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What is the true nitrogenase reaction? A guided approach

Rossana Pesi

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 2015

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A Conserved Acidic Motif in the N-Terminal Domain of Nitrate Reductase Is Necessary for the Inactivation of the Enzyme in the Dark by Phosphorylation and 14-3-3 Binding1

Emmanuelle Noel

Plant Physiology, 1999

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Evidence for nifU and nifS Participation in the Biosynthesis of the Iron-Molybdenum Cofactor of Nitrogenase

Leonardo Curatti

Journal of Biological Chemistry, 2007

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Nitrogenase (a Key Enzyme): Structure and Function

Devendra Choudhary

Soil Biology

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Promoter mutations that allow nifA-independent expression of the nitrogen fixation nifHDKY operon

Judith Berman

Proceedings of the National Academy of Sciences, 1983

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Exploring new frontiers of nitrogenase structure and mechanism

Robert Szilagyi

Current Opinion in Chemical Biology, 2006

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Energy Transduction in Nitrogenase

Simone Raugei

Accounts of chemical research, 2018

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Insights into the role of nucleotide-dependent conformational change in nitrogenase catalysis: Structural characterization of the nitrogenase Fe protein Leu127 deletion variant with bound MgATP

Robert Szilagyi

Journal of Inorganic Biochemistry, 2006

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Insights into substrate binding at FeMo-cofactor in nitrogenase from the structure of an α-70< sup> Ile MoFe protein variant

Ranjana Sarma

Journal of inorganic …, 2010

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