Antimicrobial potentials of plant extracts against drug resistant bacteria (original) (raw)
Related papers
2021
Interest in medicinal plants showing synergism with antibiotics or as an alternative to antibiotics as possible industrial product is receiving major importance. Medicinal plants are finding their way into pharmaceuticals, cosmetics, and neutraceuticals. Long before mankind discovered the existence of microbes, the idea that certain plants had healing potential, indeed, that they contained what we would currently characterize as antimicrobial principles, was well accepted. Since antiquity, man has used plants to treat common infectious diseases and some of these traditional medicines are still included as part of the habitual treatment of various maladies. Today’s ,microbial infections ,resistance to antibiotic drugs have been the biggest challenges, which threaten the health of societies. It has become widely recognized that antimicrobial resistance(AMR) is one of the biggest health threats that mankind faces encompassing huge health and economic burdens on governments and societie...
Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria
Brazilian Journal of Microbiology, 2000
The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. In addition, the possible synergistic effects when associated with antibiotics were studied. Extracts from the following plants were utilized: Achillea millifolium (yarrow), Caryophyllus aromaticus (clove), Melissa offficinalis (lemon-balm), Ocimun basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis (rosemary), Salvia officinalis (sage), Syzygyum joabolanum (jambolan) and Thymus vulgaris (thyme). The phytochemicals benzoic acid, cinnamic acid, eugenol and farnesol were also utilized. The highest antimicrobial potentials were observed for the extracts of Caryophyllus aromaticus and Syzygyum joabolanum, which inhibited 64.2 and 57.1% of the tested microorganisms, respectively, with higher activity against antibiotic-resistant bacteria (83.3%). Sage and yarrow extracts did not present any antimicrobial activity. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibiotic-resistant bacteria. The results obtained with Pseudomonas aeruginosa was particularly interesting, since it was inhibited by clove, jambolan, pomegranate and thyme extracts. This inhibition was observed with the individual extracts and when they were used in lower concentrations with ineffective antibiotics.
International Journal of Herbal Medicine, 2019
The effective prevention and treatment of an ever-increasing range of infections caused by bacteria, parasites, viruses and fungi, and at country level worldwide both in humans and animals was endangered by the emergence of conventional antimicrobial resistance. As result, the world interestingly, forward-looking to the natural products of medicinal plants as alternative remedies in the field of medical sciences in view of the novel molecules delivered by this discipline of science. In spite of the fact that, traditional medicinal healers have used medicinal plants for treatment of different ailments for centuries, there has always been a frequently asked question about their therapeutic efficacy, validation and standardization. Therefore, the main objective of this paper is to review and enlighten challenges, opportunities and future prospects of antimicrobial activities of medicinal plants as alternative solutions to combat antimicrobial resistance worldwide in humans as well as animals. Moreover, a continuous and progressing systematic researches and investigation using standardized extract develop through advanced analytical procedures need to be conducted to prove the biological ingredients and test the safety, efficiency and to determine the types of compounds responsible for the antimicrobial effects of these medicinal plants will be desirable.
The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and resistant microorganisms. In addition, the possible synergistic effects when associated with antibiotics were studied. Extracts from the following plants were utilized: Achillea millifolium (yarrow), Caryophyllus aromaticus (clove), Melissa offficinalis (lemon-balm), Ocimun basilucum (basil), Psidium guajava (guava), Punica granatum (pomegranate), Rosmarinus officinalis (rosemary), Salvia officinalis (sage), Syzygyum joabolanum (jambolan) and Thymus vulgaris (thyme). The phytochemicals benzoic acid, cinnamic acid, eugenol and farnesol were also utilized. The highest antimicrobial potentials were observed for the extracts of Caryophyllus aromaticus and Syzygyum joabolanum, which inhibited 64.2 and 57.1% of the tested microorganisms, respectively, with higher activity against antibiotic-resistant bacteria (83.3%). Sage and yarrow extracts did not present any antimicrobial activity. Association of antibiotics and plant extracts showed synergistic antibacterial activity against antibiotic-resistant bacteria. The results obtained with Pseudomonas aeruginosa was particularly interesting, since it was inhibited by clove, jambolan, pomegranate and thyme extracts. This inhibition was observed with the individual extracts and when they were used in lower concentrations with ineffective antibiotics.
Development of botanicals to combat antibiotic resistance
Journal of Ayurveda and integrative medicine, 2017
The discovery of antibiotics in the previous century lead to reduction in mortality and morbidity due to infectious diseases but their inappropriate and irrational use has resulted in emergence of resistant microbial populations. Alteration of target sites, active efflux of drugs and enzymatic degradations are the strategies employed by the pathogenic bacteria to develop intrinsic resistance to antibiotics. This has led to an increased interest in medicinal plants since 25-50% of current pharmaceuticals are plant derived. Crude extracts of medicinal plants could serve as an alternate source of resistance modifying agents owing to the wide variety of secondary metabolites. These metabolites (alkaloids, tannins, polyphenols etc.) could act as potentials for antimicrobials and resistance modifiers. Plant extracts have the ability to bind to protein domains leading to modification or inhibition protein-protein interactions. This enables the herbals to also present themselves as effectiv...
The outcome of in vitro interaction of standard antibiotics with ethanolic leaf extracts of three medicinal plants (Picralima nitida, Chromolaena odorata and Aspilia africana) against antibiotic resistant bacteria was investigated by agar disc diffusion methods and macrobroth dilution techniques. At least three isolates each of Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa that were resistant to more than two of the following antibiotic discs were used: erythromycin, chloramphenicol, tetracycline, norfloxacin and ciprofloxacin. A subinhibitory concentration (1/4th MIC) of the plant extracts were combined with the antibiotics against the resistant bacteria isolates using both antibiotic disc diffusion technique and macrobroth dilution test. The extract of A. africana enhanced the activities of the test antibiotics (except erythromycin) against resistant E. coli, and also enhanced the activities of ciprofloxacin, norfloxacin and chloramphenicol against antibiotic resistant P. aeruginosa. It also enhanced the activities of tetracycline and norfloxacin against antibiotic resistant S. aureus. The extract of C. odorata greatly enhanced the activities of the antibiotics (except erythromycin) against the resistant P. aeruginosa and also potentiated the activities of chloramphenicol and tetracycline against resistant S. aureus. Although the extract of P. nitida potentiated few antibiotics such as tetracycline and chloramphenicol against resistant E. coli, the extract exhibited high levels of antagonism with ciprofloxacin and norfloxacin against almost all the test bacteria. These results suggest that extracts of C. odorata and A. africana could be good sources of multidrug resistance inhibitors, and indicate that indiscriminate co-administration of antibiotics with some herbal drugs such as those from P. nitida could be therapeutically wasteful. Their use in combination with conventional antibiotics should be further studied for in vivo activities. This may lead to the development of much needed drug enhancing preparations.
Malaysian Journal of Microbiology, 2015
Aims: To evaluate the possible in vitro interaction between methanolic extract of root of Adiantumcapillus-veneris and certain known antimicrobial drugs i.e. Oxacillin, Ceftazimide, Cefriaxone, Ofloxacin, Meropenem, Erythromycin, Cefuroxime, Cefoxitin, Cefotaxime and Ampicillin. Methodology and results: The study was carried out against ten bacterial strains (Staphylococcus aureus, S. epidermidis, Salmonella typhi, Klebsiella pneumoniea, Shigella dysentriea, Proteus vulgaris, Pseudomonas aeruginosa, Providencia species, Citrobacter freundii and Escherichia coli isolated from urine, pus and blood samples. Both disc diffusion and well diffusion methods were used to determine antimicrobial activity of plant extract in combination with antibiotics. Antimicrobial sensitivity showed that Meropenem was the most effective antibiotic with zone of inhibition (ZI) of 25-33 mm among all tested antibiotics followed by Ofloxacin (10-26.5 mm), Ceftriaxone (8-20 mm), while Oxacillin showed no activity against almost all bacterial strains. The study showed that most bacterial strains were resistant to most of the antibiotics used, ranging from 20-60%. The methanolic extract (mEXT) of A. capillus-veneris used alone was active against most of the bacterial isolates with maximum activity against E. coli with 16 mm ZI. The study also indicated that there was an increased activity in case of combination of mEXT with antibiotics. The combined effects of plant extract with antibiotics were synergistic against most of the bacterial strains. The mEXT showed maximum synergistic effect with Ceftazimide with ZI of 42 mm followed by Meropenem (40 mm) and Ceftriaxone (28 mm) against multidrug-resistant (MDR) bacterial strains. Conclusion, significance and impact of study: The data suggests that plant extract could be used as alternative to antibiotics. These results give scientific backing that combination between plant extract and antibiotics would be useful in fighting the emerging drug-resistant bacterial pathogens.
Antibiotics
The unprecedented use of antibiotics that led to development of resistance affect human health worldwide. Prescription of antibiotics imprudently and irrationally in different diseases progressed with the acquisition and as such development of antibiotic resistant microbes that led to the resurgence of pathogenic strains harboring enhanced armors against existing therapeutics. Compromised the treatment regime of a broad range of antibiotics, rise in resistance has threatened human health and increased the treatment cost of diseases. Diverse on metabolic, genetic and physiological fronts, rapid progression of resistant microbes and the lack of a strategic management plan have led researchers to consider plant-derived substances (PDS) as alternative or in complementing antibiotics against the diseases. Considering the quantitative characteristics of plant constituents that attribute health beneficial effects, analytical procedures for their isolation, characterization and phytochemica...