+Vulvovaginal+candidiasis+clinical+signs,+and+distribution+of+Candida+species (original) (raw)
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Universal Journal of Pharmaceutical Research, 2021
Background and objectives: Vulvovaginal candidiasis is known to be a global issue of concern due to its association with economic costs, sexually transmitted diseases, and the escalation of genital tract infections. This study aimed to determine the prevalence, species distribution and risk factors associated with Candida species causing vulvovaginal candidiasis. Subjects and Methods: Non-pregnant women attending routine antenatal visits at Al-Olaifi-Family Center in Sana'a were enrolled in a cross-sectional study conducted from June 2018 to March 2019. Laboratory work was carried out at the National Center of Public Laboratories (NCPHL). Vaginal swabs were sampled from participants after oral consent was obtained. The swabs were inoculated in Sabouraud glucose agar supplemented with chloramphenicol and incubated at 37°C for 24 to 48 h under aerobic conditions in order to perform a fungal culture. Candida species were determined by culturing on HiCrome Candida differential Aga...
2015
Background: The prevalence of vulvovaginal candidiasis (VVC) caused by Candida non-albicans (CNA) species has increased dramatically in the recent decades. This study aimed to identify and to test antifungal susceptibility pattern of Candida species isolated from vaginal swabs of women with VVC, and to evaluate the performance of the commercially available Integral System Yeasts Plus (ISYP) for the identification of Candida species. Methods: High vaginal swabs (n=72) were collected from women presented with vaginitis during the period from February to December 2013. Swabs were cultured on Sabouraud Dextrose Agar, Blood agar, and Gram stained smears were examined. Identification was done by ISYP and nested PCR, and antifungal susceptibility testing of isolates was done by ISYP. Clinical, Demographic data, and risk factors of women with respect to identified species were also evaluated. Recurrent VVC (RVVC) accounted for 15/20 (75%) of cases. Resistance to clotrimoxazole was significa...
Conventional and Molecular Identification, Incidence and Species Distribution of Candida Associated with Vaginal Candidiasis among Women Attending Gynecological Clinic at Duhok Province, Kurdistan- Iraq.
Vaginal Candidiasis is one of the most common sources of inflammation of the vagina in patient women attending gynecological clinics. The present study aimed to isolate and identify the Candida species in pregnant and non-pregnant women of different age groups attending maternity hospitals at Duhok, Iraq, using conventional and molecular methods. Vaginal swab samples were collected from 385 women aged 15-60 years with suspected vulvovaginal candidiasis. All swab samples were initially cultured on Sabouraud's dextrose agar (SDA) supplemented with 0.05% (Weight/Volume) chloramphenicol and incubated at 37ºC for 48 hours. For the presumptive identification of Candida species, all growing colonies were sub-cultured on Harlequin TM Candida Chromogenic agar (CHROM agar Company, Acumedia Neogen, UK) incubated at 35ºC for 48 hours. Molecular identification was achieved by sequencing the ITS1 and ITS4 regions of rDNA. Four Candida species were identified by both conventional and molecular methods. Based on PCR detection, C. albicans (47%) was the most common species, followed by C. glabrata (36.2%), then C. krusei (7.4%) while C. tropicalis (2.2%) showed the least incidence. Vaginal candidiasis is a common problem among women in Duhok city and was more common in age groups of 15-36 years of both pregnant and non-pregnant women. Sensitive Chromogenic agar for detection of pecies showed 91.7%, while the PCR-based technique was 97.49%.
Prevalence of Vulvovaginal Candidiasis in Ahvaz, Southwest Iran: A Semi-Large Scale Study
Jundishapur Journal of Microbiology
Background: Candida vaginitis or vulvovaginal candidiasis (VVC) is the most common mucosal infection of the female genital tract caused by different species of Candida. Despite several antifungal therapies and personal hygiene practices, VVC has remained an important public health problem affecting millions of women worldwide. Objectives: The aim of this study was to determine the prevalence of VVC and the frequency of causative agents in women of different age groups referring to the midwifery clinics in Ahvaz, Iran, from January 2017 to March 2018. Methods: Samples were obtained from 493 women aged 15-64 years with signs and symptoms of VVC using endocervical swabs. All collected samples were cultured on CHROMagar Candida plates and incubated at 35°C for 24-72 h. Various Candida sp. were initially identified using morphologic characteristics and physiologic features, and finally confirmed with PCR-RFLP. Results: Totally 196 (39.76%) cases were diagnosed as VVC, of which nine (4.6%) were as recurrent vulvovaginal candidiasis. More than half of the cases were in the age group of 21-30 years. Candida albicans was the most commonly identified species (71.1%), followed by C. glabrata (20.4%) and other non-C. albicans species (8.6%). Infection in pregnant women decreased in the third trimester compared to the first and second trimesters of pregnancy. Conclusions: Vulvovaginal candidiasis is a relatively common gynecologic problem in Ahvaz. Although the frequency of non-C. albicans species in VVC has increased, C. albicans is still the predominant species.
Isolation of Different Species of Candida in Patients With Vulvovaginal Candidiasis From Sari, Iran
Jundishapur Journal of Microbiology, 2015
Background: Vulvovaginal Candidiasis (VVC) is a frequent, complex and cumbersome condition that can cause physical and psychological distress for the involved individual. Candida albicans was reported as the most common agent of VVC yet it seems that we are recently encountering changes in the pattern of Candida species in VVC. Objectives: In this study we assessed different species of Candida isolated from patients with VVC, residing in Sari, Iran. Patients and Methods: Two hundred and thirty-four patients with vulvovaginitis were enrolled in this study. Samples were collected by a wet swab. Each vaginal swab was examined microscopically and processed for fungal culture. The identification of Candida species was done by morphological and physiological methods such as culture on CHROMagar Candida media and sugar assimilation test with the HiCandida identification kit (HiMedia, Mumbai, India). Results: Out of 234 patients with vulvovaginitis, 66 (28.2%) patients showed VVC. Of these patients, 16 (24.2%) had recurrent VVC (RVVC). The age group of 20 -29 year-olds had the highest frequency of VVC (48.5%). Erythema concomitant with itching (40.9%) was the most prevalent sign in VVC patients. Fifty-seven (86.4%) of the collected samples had positive results from both microscopic examination and culture. In total, 73 colonies of Candida spp. were isolated from 66 patients with VVC. The most common identified species of Candida were C. albicans (42.5%), C. glabrata (21.9%) and C. dubliniensis (16.4%). In patients with RVVC and patients without recurrence, C. albicans and non-albicans species of Candida were frequent species, respectively. Conclusions: The results of our study showed that non-albicans species of Candida are more frequent than C. albicans in patients with VVC. This result is in line with some recent studies indicating that non-albicans species of Candida must be considered in gynecology clinics due to the reported azole resistance in these species.
2020
1. Department of Parasitology and Mycology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran 2. Department of Obstetrics and Gynecology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran 3. Maternal, Fetal and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran 4. Kamali Hospital, Alborz University of Medical Sciences, Karaj, Tehran 5. Yas Hospital, Tehran University of Medical Sciences, Tehran, Iran 6. Shohada Hospital, Qom University of Medical Sciences, Qom, Iran
Prevalence of Candida Albicans Among Women Diagnosed with Vaginosis in Tripoli
KEY WORDS: Objective: To determine the prevalence and age distribution of different Candida species among women diagnosed with vaginosis. Material and Method: Microbiological examination of high vaginal swabs obtained from 206 women who complained of recurrent vaginal discharge. Gram stain and wet mount preparation, culture, germ tube test, and a special API system were used for identification of Candida species. Result: Overall 71% of patients with vaginal discharge were infected with vaginitis. Candidiasis was found in 25% of vaginitis cases, C. albicans was the predominant species, 50% compared with Torulopsis glabrat isolated in 25%, and C. tropicals 7.6 %, while C. mongolia, C. krusei, and C. prpasilosis were all 3.8 % respectively, and other species only 5.7 %. C. albicans was isolated in 54% of age group 25-34 years, while T. glabrata was isolated in 54% of age group 35-44 years. Conclusion: Yeast infection caused by C. albicans represented the highest percentage of candidiasis among women diagnosed with vaginosis, with almost 54% among age group 25-34 years. We suggest that performing the aetiological diagnosis and genotyping of strains associated with candidiasis is necessary in order to institute appropriate therapeutic and preventive measures.
Vulvovaginal candidiasis (VVC) or Candida vaginitis is a common fungal infection among adult women during reproductive ages and is the second most frequent infection of the female genital tract. This study was designed to determination of clinical findings and the species generating Vulvovaginal candidiasis in women referred to gynecology clinic in Chabahar. In this study all of 400 vaginal samples were examined with the wet smear methods and cultured on sabouraud dextrose agar (SDA) and (CMA). Germ tube te st and sugar assimilation test (API) were carried out to differentiate the Candida species in Chabahar in 2014. The data analysis was done using the SPSS software version 18.0 and chi - square and T student tests. In this study the prevalence of Vulvovaginal candidiasis (VVC) was 45.8% and the Candida albicans with (80.87%) and Candida non - albicans with (19.13%) were reported as generated species of Vulvovaginal candidiasis respectively. However, there was no statistically significant correlation between the demographic factors, age at marriage and educational level with infection. But there was statistically significant relationship between burning, itching, malodorous discharge, vaginal parity, pH of the vagina, cervix appearance, consistency and color disch arge with infection. Considering the prevalence of non - albicans species is increasing due to inappropriate antifungal therapy and on the other hand, recurrent infections, is also rising. It is recommended, vaginal Candida culture method (with high sensitiv ity) should be performed routinely in medical laboratory and with the determination of species and drug susceptibilities, appropriate medication is prescribed
Revista iberoamericana de micología, 2004
Vulvovaginal candidiasis (VVC) is an infection caused by abnormal yeast growth in the mucosa of the female genital tract which is commonly diagnosed in gynecology. The aim of this study was to correlate the frequency of yeasts and their respective species in asymptomatic women with different clinical manifestation of VVC; evaluate possible relationships between number of fungus colonies and symptoms in this pathology. All patients who visited the laboratory within a period of five months, for routine examinations of vaginal secretion, independent of the presence or absence of symptoms of VVC were included in this study. Of these, women with immunodeficiency or with an infection of the genital tract by another agent were excluded. Candida albicans was the most frequently yeast isolated (60%). Among non-C. albicans yeasts, 61.5% were isolated of the asymptomatic women, 38.7% from patients with VVC and 11.1% of those from patients with RVVC. C. albicans was associated with symptoms of ...