Quality assessment of market samples of crude drugs with particular refence to Asrol (Rauvolfia serpentina Benth ex. Kurz) (original) (raw)
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International Journal of Science and Research, 2019
The extraordinary medicinal properties of Rauvolfia serpentine (Asrol) have brought this Unani drug plant to the attention of the medical world. All over the world, medicinal plants are occupying the main role in the traditional system of medicines which includes Siddha, Ayurveda, Unani, Yoga and Naturopathy. In the modern era, Rauvolfia serpentine (Asrol) is used as an effective Antihypertensive. Medical fraternity continues research on it. It helps to reduce blood pressure (FisharuddamQawi) by dilating blood-vessels(1), depresses activity of central nervous system(2) Psychiatric disorders (NafsiyatiAmraz) and acts as a hypnotic (munavem).its use in schizophrenia (Malan khuliay), insanity (junoon), Sara (epilepsy), Hysteria (AkhtanaqurReham) (3)
Fitoterapia, 2016
Herbal medicines are the most globally used type of medical drugs. Their high cultural acceptability is due to the experienced safety and efficiency over centuries of use. Many of them are still phytochemically less-investigated, and are used without standardization nor quality control. Choosing SIROP KILMA, an authorized Congolese antimalarial phytomedicine, as a model case, our study describes an interdisciplinary approach for a rational quality assessment of herbal drugs in general. It combines an authentication step of the herbal remedy prior to any fingerprinting, the isolation of the major constituents, the development and validation of an HPLC-DAD analytical method with internal markers, and the application of the method to several batches of the herbal medicine (here KILMA) thus permitting the establishment of a quantitative fingerprint. From the constitutive plants of KILMA, acteoside, isoacteoside, stachannin A, and pectolinarigenin-7-O-glucoside were isolated, and acteoside was used as the prime marker for the validation of an analytical method. This study contributes to the efforts of the WHO for the establishment of standards enabling the analytical evaluation of herbal materials. Moreover, the paper describes the first phytochemical and analytical report on a marketed Congolese phytomedicine.
International Journal of Applied Pharmaceutics, 2018
Objective: Easy and precise methods were developed for estimation of aspirin (ASP), impurities from such as salicylic acid (SAL) and heavy metal ions (HMI) in ASP tablets that available in the Iraqi's market using High-performance liquid chromatography (HPLC), UV-VIS spectrophotometry and atomic absorption spectrophotometric (AAS). Methods: HPLC separation was carried out using C18 as stationary phase and acetonitrile (ACN): water in the ratio of (10: 90 v/v) as a mobile phase for HPLC method and as a solvent for UV-VIS spectrophotometric for quantitative ASP and SAL at 254 nm for HPLC, 226 and 296 nm for UV measurements. AAS was used for HMI determination. Results: ASP and SAL gave absorbance maxima at 226 and 296 nm in ACN: H2O solvent. The Beer's law was obeyed in the range of 0.05-20 for ASP and 0.02-8 µg/ml for SAL. Correlation coefficients (R 2) were 0.9996 and 0.9992 for ASP and SAL respectively, for HPLC and LOD value was 0.006 for ASP and 0.004 μg/ml for SAL. The % recovery for the developed method was found to be in the range of (98.80 to 101.26%) and (98.67 to 103.33%) for ASP and SAL respectively, within the acceptable range, that approved by world health organization (WHO). Conclusion: The proposed method can help research studies, quality control and routine analysis with lesser resources available. The results of the assay of pharmaceutical formulation of the developed method are highly reliable and reproducible and is in good agreement with the label claim of the medicines.
Quality evaluation of Rauvolfia serpentina by physicochemical analysis
Journal of Pharmacognosy and Phytochemistry
Objective: Rauvolfia serpentina plant species is used since ancient time. Now days it has been became endangered plant species in India due to climacteric changes and increased demand in drug industry. Hence comparative physiochemical analysis of wild and cultivated species is necessary. Rauvolfia serpentina mother tincture is having specific target action on hypertension. Thus before initiating phytochemical standardization and in vivo study, validation of manufacturing process and physicochemical analysis is essential. Methods: According to HPI guidelines, two samples of Rauvolfia serpentina mother tincture were prepared from wild and cultivated species in GMP certified homoeopathic manufacturing unit. Further validation and comparative physicochemical analysis were done for both samples. Results: The physicochemical parameters of Rauvolfia serpentina mother tincture prepared from wild and cultivated species were almost alike. Conclusion: This analytical data will be possibly considered as quality standard for Rauvolfia serpentina mother tincture prepared from wild and cultivated species in further research. The process of manufacturing and physicochemical values of wild and cultivated samples are validated and established in the study.
Molecules
The leaves of Asphodelus bento-rainhae subsp. bento-rainhae, an endemic Portuguese species, and Asphodelus macrocarpus subsp. macrocarpus have been used as food, and traditionally as medicine, for treating ulcers, urinary tract, and inflammatory disorders. The present study aims to establish the phytochemical profile of the main secondary metabolites, together with the antimicrobial, antioxidant and toxicity assessments of both Asphodelus leaf 70% ethanol extracts. Phytochemical screenings were conducted by the TLC and LC-UV/DAD-ESI/MS chromatographic technique, and quantification of the leading chemical classes was performed by spectrophotometric methods. Liquid-liquid partitions of crude extracts were obtained using ethyl ether, ethyl acetate, and water. For in vitro evaluations of antimicrobial activity, the broth microdilution method, and for the antioxidant activity, the FRAP and DPPH methods were used. Genotoxicity and cytotoxicity were assessed by Ames and MTT tests, respecti...
2012
In the last few years, aspirin has become a life sa ver against cardiovascular accident. This study was carried out to determine possible dose variations in the amount of active constituent (acetylsalicylic acid) in the various brands of aspirin drugs marketed in Maiduguri metropolitan co uncil. In this study, ten different brands of 300mg aspirin tablet were randomly sampled from different pharmac y shops; identification test was performed on each sample to confirm the presence of the active drug (acetylsali cylic acid), and each of these samples was subjecte d to a rigorous quantitative analysis using HPLC and UV-spectrophot ometer to determine the amount of the active drug. From the analysis, out of the ten brands of aspirin worked o n, only Bond aspirin(41.7%) has less than 90% of ac tive drug in UV-spectroscopy and Odesprin(112%) has more than 11 0% while Biopharma(99.77%), Kp(90%), Kunimed(100%), Maxprin(105%), Michelleaspirin(95%), Nemeprin(100%) ,Propon(93%) and Stop-eke(105...
Standardization, evaluation and quantification of herbal drugs by various analytical methods
International Journal of Ayurvedic Medicine
This review article encompasses the various evaluation parameters of standardization of different medicinal plants and its constituents. In today’s life, more and more people of the world are turning towards the use of natural or herbal products as these have very less or negligible side effects. As the use of herbal medicinal products is increasing day by day, the questions regarding their quality are also arising. This is due to lack of parameters for the standardization of these products. There is a need to set different parameters or methods that can help to standardize the herbal medicinal plant materials. Various methods like physical, chemical, biological is used for standardization and quality evaluation of herbal medicinal plants. These methods can act as a basic tool for the quality evaluation of herbal plant materials. Different parameters of standardization are the fundamental tool for evaluating and assuring the quality of the herbal plant material and its products. Thi...
Assessment of bioactive ingredient of five brands of Aspirin tablets in some pharmacies in Libya
2014
Acetylsalicylic acid is the bioactive ingredient of Aspirin. Active ingredient of five brands of Aspirin was assessed by using bac k titration method. The values for the % content showed that Brands 01, 03 and 05 had 106 %, 104% and 101%, respectively, comply within specified range of 90% to 110% as stated in USP standard, however, Brand 02 and Brand 04 showed values 86.66% and 120%, respectively, which did not meet with the USP standard limit hence both Brands are rejected as pe r testing and not recommended for the sell in Pharmacy Stores and use in med practice as per USP standard limit.
Major bioactive chemical compounds in Astragali Radix samples from different vendors vary greatly
PLOS ONE, 2021
The worldwide traditional Chinese medicine (TCM) herbs sales figures have increased considerably to 50 billion US$ (2018). Astragali Radix (AR) is amongst the most often sold TCM herbs; sales in the European Union (EU) need European Medicines Agency (EMA) approval. However, comparisons of characteristic bioactive molecules concentrations in AR from different EU vendors are lacking. This study uses liquid chromatography-tandem mass spectrometry (LC-MS/MS) with standard addition to evaluate the influence of different sample and preparation types and ammonia treatment on bioactive molecules concentrations in AR. We also compare AR samples from different EU-vendors. Astragaloside IV (AG-IV), ononin and calycosin 7-O-β-D-glucoside concentrations were higher in root powder samples when extracted with boiled water than with ultrasonication using 70% methanol. AG-IV content was by far the highest in granulates from vendor 1 (202 ± 35 μg/g) but very low in hydrophilic concentrates from vendo...