Profiling serum biomarkers in patients with COPD: associations with clinical parameters (original) (raw)

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COPD Exacerbation Biomarkers Validated Using Multiple Reaction Monitoring Mass Spectrometry

PloS one, 2016

Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) result in considerable morbidity and mortality. However, there are no objective biomarkers to diagnose AECOPD. We used multiple reaction monitoring mass spectrometry to quantify 129 distinct proteins in plasma samples from patients with COPD. This analytical approach was first performed in a biomarker cohort of patients hospitalized with AECOPD (Cohort A, n = 72). Proteins differentially expressed between AECOPD and convalescent states were chosen using a false discovery rate <0.01 and fold change >1.2. Protein selection and classifier building were performed using an elastic net logistic regression model. The performance of the biomarker panel was then tested in two independent AECOPD cohorts (Cohort B, n = 37, and Cohort C, n = 109) using leave-pair-out cross-validation methods. Five proteins were identified distinguishing AECOPD and convalescent states in Cohort A. Biomarker scores derived from this model...

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Profiling serum biomarkers in patients with COPD: associations with clinical parameters (original) (raw)

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