Treadmill Exercise Training Improves Vascular Endothelial Growth Factor Expression in the Cardiac Muscle of Type I Diabetic Rats (original) (raw)
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Cardiovascular Diabetology, 2008
Background: Diabetes has negative, and exercise training positive, effects on the skeletal muscle vasculature, but the mechanisms are not yet fully understood. In the present experiment the effects of running exercise on the mRNA expression of pro-and antiangiogenic factors were studied in healthy and diabetic skeletal muscle. The responses in capillaries and muscle fibers, collected from the muscle with laser capture microdissection, were also studied separately.
Journal of Cardiovascular and Thoracic Research, 2021
Introduction: This research investigates the impact of insulin-like growth factor-I (IGF -I)and exercise on mediators associated with angiogenesis (VEGF-A, TSP-1, and NF-кβ) and capillarization status of the diabetic rats’ hearts. Methods: Splitting of forty Wistar male rats into five groups occurred as following: control,diabetes, diabetes+IGF-I, diabetes+exercise, and diabetes+exercise+IGF-I.Through intraperitoneal administration of 60 mg/kg streptozotocin, the condition of Type 1diabetes was escalated. After four weeks of treatment with IGF-I (2 mg/kg/day) or treadmill exercise (17 m/min, zero degrees slope, 30 min/day), in the heart, microvascular density and protein levels of VEGF-A, TSP-1, and NF-кβ were determined by H & E staining and ELISA,respectively. Results: Within the diabetic group, observations present a significant decrease in VEGF-A and MVD levels, whereas an increase in the TSP-1 and NF-Κb levels. While these impacts were reversed by either IGF-I or exercise treat...
Angiogenic growth factor responses to long-term treadmill exercise in mice
Indian journal of physiology and pharmacology
We sought to determine whether VEGF and other angiogenic growth factors and their receptors might be subject to negative feedback regulation during two weeks of treadmill-exercise conditioning in inbred strains of mice. C57BL/6 mice exhibited greater VEGF mRNA and protein responses in gastrocnemius muscle to a single bout of treadmill exercise compared to BALB/c mice. The patterns of VEGF, VEGFR1, VEGFR2, Ang2 and Tie2 mRNA expression in gastrocnemius muscles of C57BL/6 mice during long-term exercise support the hypothesis that they may be subject to negative feedback regulation. The combination of expression patterns for growth factors and their receptors suggests that multiple layers of control mechanisms may exist to prevent angiogenesis following a single bout of exercise and to promote angiogenesis following long-term exercise.
We evaluated vascular endothelial growth factor (VEGF) expression in cultured rabbit corneal cells (epithelium, stromal cells and endothelium) following exposure to linoleic acid hydroperoxide (18: 2-OOH/LHP). Tissues were isolated from the superior quadrant of rabbit cornea, transferred to a 6-well dish, cultured to 80% confluence in Rabbit Corneal Cell Growth Medium 2 (RCGM2) or Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), respectively, and harvested at passage 2 or 3. Each specimen was maintained in 2% FBS for 24 hr, then LHP was added to the medium at concentrations ranging from 10-10M to 10-4M. Twenty-four hours later, VEGF was measured in the medium using an immunoassay kit. LHP at 10-5M increased VEGF in stromal cells from a basal level of 1106 to 2300 pg/mg protein. A minor increase by LHP was observed in epithelial cells and endothelial cells at 10-8M. These data extend our previous report of VEGF up-regulation in the LHP-induced, non-inflammatory neovascular corneal rabbit model. Stromal cells are thought to be the main source of this angiogenic cytokine.
The Effect of Interval and Continuous Training on Angiogenesis Factor in Type2 Diabetic Wistar Rats
Iranian journal of diabetes and obesity
Objective: The equilibrium of angiogenesis stimulus agents and angiogenesis inhibitory agents is an important factor in the increase of diabetic cardiomyopathy. This research aimed to survey the result of eight weeks of high-intensity interval training (HIIT) and moderate continuous (MICT) on the myocardium angiogenesis agent and histological alterations within male diabetic rats. Materials and Methods: 32 male Wistar rats did casually selected within 4 groups: health without exercise control, diabetic without exercise (D), D + HIIT, and D+ MICT groups. Diabetes type 2 produced with high-fat food for two weeks and an only dose of STZ. After approval of type 2 diabetes, subjects did direct to HIIT (90 -95 percent of VO2max), and MICT (50 to 65 percent of VO2max) exercise program five times per week during eight weeks. Western blotting methods were utilized for the exposure of protein synthesis of Vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-beta (TGF-β1), and...
Biomolecules, 2021
Exercise can ameliorate cardiovascular dysfunctions in the diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of the present study was to determine the impact of endurance training on expression of angiogenesis-related genes in cardiac tissue of diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N = 10) including diabetic training (DT), sedentary diabetes (SD), and sedentary healthy (SH), in which diabetes was induced by a single dose of streptozotocin (50 mg/kg). Endurance training (ET) with moderate-intensity was performed on a motorized treadmill for six weeks. Training duration and treadmill speed were increased during five weeks, but they were kept constant at the final week, and slope was zero at all stages. Real-time polymerase chain reaction (RT-PCR) analysis was used to measure the expression of myocyte enhancer factor-2C (MEF2C), histone deacetylase-4 (HDAC4) and Calmodulin-dependent prote...
The FASEB Journal, 2006
Diabetes alters microvascular structure and function and is a major risk factor for cardiovascular diseases. In diabetic skeletal muscle, impaired angiogenesis and reduced VEGF-A expression have been observed, whereas in healthy muscle exercise is known to have opposite effects. We studied the effects of type 1 diabetes and combined exercise training on angiogenic mRNA expression and capillarization in mouse skeletal muscle. Microarray and real-time PCR analyses showed that diabetes altered the expression of several genes involved in angiogenesis. For example, levels of proangiogenic VEGF-A, VEGF-B, neuropilin-1, VEGFR-1, and VEGFR-2 were reduced and the levels of antiangiogenic thrombospondin-1 and retinoblastoma like-2 were increased. Exercise training alleviated some of these changes, but could not completely restore them. VEGF-A protein content was also reduced in diabetic muscles. In line with the reduced levels of VEGF-A and other angiogenic factors, and increased levels of angiogenesis inhibitors, capillary-to-muscle fiber ratio was lower in diabetic mice compared to healthy controls. Exercise training could not restore capillarization in diabetic mice. In conclusion, these data illustrate that type 1 diabetes is associated with reduced skeletal muscle capillarization and the dysregulation of complex angiogenesis pathways.-Kivelä, R., Silvennoinen, M., Touvra, A.-M., Lehti, T. M., Kainulainen, H., Vihko, V. Effects of experimental type 1 diabetes and exercise training on angiogenic gene expression and capillarization in skeletal muscle. FASEB J. 20, E921-E930 (2006) Key Words: angiogenesis ⅐ hyperglycemia ⅐ growth factor ⅐ VEGF E921 0892-6638/06/0020-0921 © FASEB E922 Vol. 20 June 2006 KIVELÄ ET AL. The FASEB Journal E923 DIABETES, EXERCISE TRAINING, AND ANGIOGENESIS Gene expressions are expressed in relation to the control group at the same time point. Statistically significant changes are color-coded: red ϭ up-regulated (two-based log of ratio (sample/control) Ն0.3 and PՅ0.0025), green ϭ down-regulated (two-based log of ratio (sample/control) Յ-0.3 and PՆ0.9975), yellow ϭ diabetes-induced change in expression was attenuated by exercise (significant change in expression in opposite direction in comparisons DT vs. D and D vs. C). Group abbreviations: D ϭ diabetic, DT ϭ diabetic trained, and T ϭ healthy trained, numbers ϭ time of the treatment in weeks.
The Chinese journal of physiology, 2008
Exercise is well-known in improving vascular functions, but the underlying mechanism has not been totally understood. The aim of this study was to examine whether single exercise session acutely enhances insulin-induced and insulin-like growth factor-1 (IGF-1)-induced vasorelaxation. Twenty-four male Wistar rats at age of 12 weeks were randomly divided into two groups, control (n = 12) and exercise (n = 12) group. The exercise group ran on a treadmill at a speed of 18 m/min for 60 min. Immediately after exercise, insulin-induced and IGF-1-induced vasorelaxant responses were evaluated by the isometric tension of aortic rings in the organ baths. The roles of phosphatidylinositol 3-kinase (PI3K) and nitric oxide synthase (NOS) in vasorelaxant responses were examined by treating selective inhibitors, such as wortmannin (an inhibitor of PI3K) and N(omega)-nitro-L-arginine methyl ester (L-NAME, a NOS inhibitor). In addition, the vascular responses to sodium nitroprusside (SNP), a NO donor...
Canadian journal of diabetes, 2016
Impaired angiogenesis in cardiac tissue is a major complication of diabetes. This study was aimed to evaluate the effects of testosterone and voluntary exercise on vascular endothelial growth factor-A (VEGF-A), stromal cell-derived factor 1a (SDF-1a) and myocardial capillary density in heart of rats with diabetes. Type 1 diabetes was induced by intraperitoneal injection of 55 mg/kg of streptozotocin in 80 male Wistar rats. After 42 days of treatment with testosterone (2 mg/kg/day) or voluntary exercise alone or in combination, angiogenesis was determined in the hearts by immunostaining for PECAM-1/CD31. The expressions of VEGF-A and SDF-1a levels in heart were also determined by the ELISA method. Our results showed that capillary density, VEGF-A and SDF-1a levels in the heart were significantly decreased in castrated rats with diabetes, whereas these effects were reversed by testosterone and exercise. Furthermore, simultaneous treatment of castrated rats with diabetes with testoster...