Upregulation of Vascular Endothelial Growth Factor Expression in the Kidney Could Be Reversed Following Treadmill Exercise Training in Type I Diabetic Rats (original) (raw)
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Diabetes, 1999
It has been suggested that the cytokine vascular endothelial growth factor (VEGF) has an important role in the pathogenesis of diabetic retinopathy, but its role in nephropathy has not been clearly demonstrated. Assessment of VEGF, 125I-VEGF binding, and vascular endothelial growth factor receptor-2 (VEGFR-2) in the kidney was performed after 3 and 32 weeks of streptozotocin-induced diabetes. Gene expression of both VEGF and VEGFR-2 was assessed by Northern blot analysis and the localization of the ligand and receptor was examined by in situ hybridization. VEGF and VEGFR-2 protein were also evaluated by immunohistochemistry. Binding of the radioligand 125I-VEGF was evaluated by in vitro and in vivo autoradiography. Diabetes was associated with increased renal VEGF gene expression. VEGF mRNA and protein were localized to the visceral epithelial cells of the glomerulus and to distal tubules and collecting ducts in both diabetic and nondiabetic rats. Renal VEGFR-2 mRNA was increased af...
gazi university journal of science, 2016
There is positiveeffect of exogenvascularendothelialgrowthfactor (VEGF) applicationtodiabeticwound.It is aimed to research the effects of topical VEGF administration on oxidative parameters in rat serum.In this study, 36 male wistar albino rats (weight: 200-250 g) were used. All rats get diabetic with streptozotocin. Dorsalateral excisional wounds (4 cm lengthy) were made on the two sides of medulla spinalis on the rats and they were randomly divided into 3 groups: untreated groups (n=12), chitosan treated (n=12)and chitosan+ VEGF (n=12)(7 ng/ml). In chitosan treated groups, wounds treated topically with equal amount blank chitosan gel. After this administration on the 3 th and 7 th days of wound,animals were sacrified. NOx, TBARS and RSH levels were measured spectrophotometrically in serum. Results were expressed as mean ± standart deviation. Mean differences were compared by Anova Variance Analysis(p<0,05). It can be thought that topical VEGF administration on the excisional wo...
Cardiovascular Diabetology, 2008
Background: Diabetes has negative, and exercise training positive, effects on the skeletal muscle vasculature, but the mechanisms are not yet fully understood. In the present experiment the effects of running exercise on the mRNA expression of pro-and antiangiogenic factors were studied in healthy and diabetic skeletal muscle. The responses in capillaries and muscle fibers, collected from the muscle with laser capture microdissection, were also studied separately.
[The role of vascular endothelial growth factor (VEGF)]
Klinika oczna
The authors present the role of VEGF in microaneurysm formation, blood-retinal barrier breakdown, development of capillary nonperfusion and retinal neovascularization in pathogenesis of diabetic retinopathy. Inhibitors of VEGF in treatment of diabetic retinopathy are presented.
Journal of Clinical Investigation, 1997
The purpose of these experiments was to investigate a potential role for vascular endothelial growth factor (VEGF) in mediating vascular dysfunction induced by increased glucose flux via the sorbitol pathway. Skin chambers were mounted on the backs of Sprague-Dawley rats and 1 wk later, granulation tissue in the chamber was exposed twice daily for 7 d to 5 mM glucose, 30 mM glucose, or 1 mM sorbitol in the presence and absence of neutralizing VEGF antibodies. Albumin permeation and blood flow were increased two-to three-fold by 30 mM glucose and 1 mM sorbitol; these increases were prevented by coadministration of neutralizing VEGF antibodies. Blood flow and albumin permeation were increased ف 2.5-fold 1 h after topical application of recombinant human VEGF and these effects were prevented by nitric oxide synthase (NOS) inhibitors (aminoguanidine and N G -monomethyl L -arginine). Topical application of a superoxide generating system increased albumin permeation and blood flow and these changes were markedly attenuated by VEGF antibody and NOS inhibitors. Application of sodium nitroprusside for 7 d or the single application of a calcium ionophore, A23187, mimicked effects of glucose, sorbitol, and VEGF on vascular dysfunction and the ionophore effect was prevented by coadministration of aminoguanidine. These observations suggest a potentially important role for VEGF in mediating vascular dysfunction induced by "hypoxia-like" cytosolic metabolic imbalances (reductive stress, increased superoxide, and nitric oxide production) linked to increased flux of glucose via the sorbitol pathway. ( J. Clin. Invest. 1997Invest. . 99:2192Invest. -2202
Background: Vascular endothelial growth factor (VEGF) expres- sion is a potent mitogen for endothelial cells that is involved in angiogenesis. Cardiac VEGF is decreased in many pathologic conditions, including diabetes mellitus and aging. Exercise training has improved VEGF expression in the aging heart. Thus, the aim of our study is to illustrate the impact of treadmill exercise training on the cardiac VEGF expression in type I diabetic rats. Methods: Twenty normal Sprague-Dawley rats and Sprague Dawley rats with streptozotocin-induced diabetes were divided into the following equal groups: sedentary control (SC), exercised control (EC), sedentary diabetic rats (SD) and exercised diabetic rats (ED). Immunohistochemistry was used to investigate VEGF expression in the cardiac tissue in each of the four different groups. Results: Cardiac VEGF expression was significantly (P < 0.05) lower in SD compared with that in SC. However, exercise training significantly (P < 0.01) enhanced VEGF expression in the cardiac tissue in ED compared with that in SD. Conclusion: Our present data suggest that treadmill exercise training improved diabetes-induced downregulation in the cardiac VEGF expression.
VEGF-induced corneal neovascularisation in a rabbit experimental model
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2010
Various conditions may cause vascularization of the normally avascular cornea. The aim of the present study was to create a reproducible experimental model that could enable the investigation of the phenomena leading to corneal vascularization. This involved creating a software to record the experimental data, enabling a subsequent digital analysis based on the growth models. The VEGF-induced pattern of neovascularization was also investigated. Twenty-seven rabbits divided in groups were used for the purposes of the present study. Some of them underwent intracorneal implants with or without vascular endothelial growth factor (VEGF) pellets, using an original microsurgical technique. Central and peripheral corneal burns were induced to other groups of animals in order to mimic the neovascularization process induced by inflammation. Finally, Dexamethasone (Maxidex) was given intraocularly, on days 1 and 3 after the onset of neovascularisation, in rabbit groups with both corneal burns ...
Serum vascular endothelial growth factor and diabetic nephropathy progression
Medicinal Chemistry Research, 2007
To determine the serum levels of vascular endothelial growth factor (VEGF) at different stages of diabetic nephropathy. Study subjects were divided into three groups: subjects in group 1 were microalbuminuric diabetic (n = 33), in group 2 subjects were normoalbuminuric diabetic group (n = 32), and group 3 was formed from nondiabetic healthy control subjects (n = 18). VEGF, microalbuminuria, HbA1c, creatinine, triglycerides, total cholesterol, fasting serum glucose, highdensity lipoprotein (HDL), and low-density lipoprotein (LDL) were measured. Serum VEGF concentrations were significantly higher in the diabetic groups than in the control group even at the normoalbuminuric stage. Serum VEGF levels were higher in the microalbuminuria group than in the normoalbuminuria group. Serum VEGF levels increased with diabetic nephropathy stage. In the diabetic group, serum VEGF appeared to be positively correlated with fasting plasma glucose, HbA1c, LDL, creatinin and microalbuminuria. Serum VEGF was found to be negatively correlated with serum HDL. We have shown that serum levels of VEGF represents an early marker of generalized vascular dysfunction, and this peptide contributes to endothelial damage in diabetes. Elevated serum VEGF levels in the normoalbuminuric stage could indicate that generalized vascular dysfunction is present even at this stage.