Selective extraction of acetic acid from the fermentation broth produced by Mannheimia succiniciproducens (original) (raw)
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Process Biochemistry, 2006
The present study deals with the development of purification and separation processes required to produce the highly purified succinic acid from the fermentation broth produced by recombinant microorganism, Mannheimia succiniciproducens. The newly developed process consists of the pretreatment process such as reactive extraction and vacuum distillation step and the crystallization process for the highly purified succinic acid production. By-produced acids were effectively removed by the reactive extraction as a primary separation. In addition, the crystallization was applied without adding any salts to produce highly purified succinic acid. The purified succinic acid, with 99.8% purity and 73.1% yield rate was obtained through this newly developed purification process. #
Separation Science and Technology, 2015
This work aims to study how to remove the organic acid by-products from simulated fermentation broth containing succinic acid by re~ctive extraction. Model solutions including single-, binary-, ternary-, and quaternary-solute solutions were used. The broths were reactively extracted using 0.25 mol TOA/kg l-octanol under pH of 2.45-6.0. The extracted broths were then distillated under vacuum-0.017 MPa with operating temperature between 45 and 65°(, Finally the distilled broths were crystallized at 4°C and pH of 2.0. The results showed that the purity and yield of succinic acid of 99.10% and 30.25%, respectively, were obtained.
Bioprocess and Biosystems Engineering, 2003
Mannheimia succiniciproducens MBEL55E isolated from bovine rumen is able to produce a large amount of succinic acid in a medium containing glucose, peptone, and yeast extract. In order to reduce the cost of the medium, whey and corn steep liquor (CSL) were used as substrates for the production of succinic acid by M. succiniciproducens MBEL55E. Anaerobic batch cultures of M. succiniciproducens MBEL55E in a whey-based medium containing CSL resulted in the production of succinic acid with a yield of 71% and productivity of 1.18 g/l/h, which are similar to those obtained in a whey-based medium containing yeast extract (72% and 1.21 g/l/h). Anaerobic continuous culture of M. succiniciproducens MBEL55E in a wheybased medium containing CSL resulted in a succinic acid yield of 69% and a succinic acid productivity as high as 3.90 g/l/h. These results show that succinic acid can be produced efficiently and economically by M. succiniciproducens MBEL55E from whey and CSL.
New reactive extraction systems for separation of bio-succinic acid
Bioprocess and Biosystems Engineering, 2011
Biotechnologically produced succinic acid has the potential to displace maleic acid and its uses and to become an important feedstock for the chemical industry. In addition to optimized production strains and fermentation processes, an efficient separation of succinic acid from the aqueous fermentation broth is indispensable to compete with the current petrochemical production processes. In this context, high molecular weight amines are known to be effective extractants for organic acids. For this reason, as a first step of isolation and purification, the reactive extraction of succinic acid was studied by mixing aqueous succinic acid solutions with 448 different amine-solvent mixtures as extraction agents (mixer-settler studies). The extraction agents consist either of one amine and one solvent (208 reactive extraction systems) or two amines and two solvents (240 reactive extraction systems). Maximum extraction yields of succinic acid from an aqueous solution with 423 mM succinic acid at pH 2.0 were obtained with more than 95% yield with trihexylamine solved in 1-octanol or with dihexylamine and diisooctylamine solved in 1-octanol and 1-hexanol. Applying these optimized reactive extraction systems with Escherichia coli fermentation broth resulted in extraction yields of 78-85% due to the increased ionic strength of the fermentation supernatant and the coextraction of other organic acids (e.g., lactic acid and acetic acid), which represent typical fermentation byproducts.
Extraction of Succinic Acid from Real Fermentation Broth by Using Emulsion Liquid Membrane Process
2018
Succinic acid is listed as one of the twelve building block chemicals based on the ease of production through a biotechnological approach and potential to derive various chemicals. The application of bio-based succinic acid is still limited due to high downstream processing costs. One of the potential methods to recover succinic acid is emulsion liquid membrane (ELM). The ELM system consists of three main liquid phases; external feed, membrane, and internal. In this study, the membrane phase was prepared by dissolving Amberlite LA2 as a carrier, sorbitan monooleate (Span 80) and polyoxyethylenesorbitan monooleate (Tween 80) as surfactants in commercial grade palm oil, while the internal phase comprised of sodium carbonate solution, Na2CO3. The influence of emulsifying time, agitation speed, and agitation time on the water-in-oil-in-water (W/O/W) emulsion stability were studied. The most stable condition was implemented on various external phase concentrations to study the extraction...
Extraction of succinic acid from real fermentation broth via emulsion liquid membrane
2018
Succinic acid is listed as one of the twelve building block chemicals based on the ease of production through a biotechnological approach and potential to derive various chemicals. The application of bio-based succinic acid is still limited due to high downstream processing costs. One of the potential methods to recover succinic acid is emulsion liquid membrane (ELM). The ELM system consists of three main liquid phases; external feed, membrane, and internal. In this study, the membrane phase was prepared by dissolving Amberlite LA2 as a carrier, sorbitan monooleate (Span 80) and polyoxyethylenesorbitan monooleate (Tween 80) as surfactants in commercial grade palm oil, while the internal phase comprised of sodium carbonate solution, Na 2 CO 3 . The influence of emulsifying time, agitation speed, and agitation time on the water-in-oil-in-water (W/O/W) emulsion stability were studied. The most stable condition was implemented on various external phase concentrations to study the extrac...
Journal of Environmental Chemical Engineering, 2020
Fermentative production of succinic acid (SA) from renewable resources such as whey is environmentally sustainable compared to petroleum-based synthesis. However, a major drawback of fermentation is the concurrent production of SA with byproducts such as lactic acid (LA), formic acid (FA) and acetic acid (AA). Therefore, appropriate downstream SA recovery and purification steps are significant in ensuring sustainable SA production. In this study, SA was fermented by Actinobacillus succinogenes and recovered in an integrated process consisting of ultrafiltration, vacuum distillation and reactive extraction. The extractant used was tri-n-octylamine (TOA) with 1-octanol as a diluent for both liquid-liquid (LLE) extraction and supported liquid membrane (SLM). The produced SA titer and yield was 11.16 g/L and 0.44 g/g, respectively. The steady state ultrafiltration permeate flux ranged from 31.18 to 33.42 L/m 2 h, and complete decolorization of the fermentation broth was achieved with 10 % (w/v) of powdered activated carbon. The extraction efficiency for LLE was 51.5 %, whereas SLM achieved 57.3 % recovery. SA exhibited transport and permeability coefficient of 0.00697 h −1 (R 2 > 0.92) and 0.08605 cm h −1 , respectively. Extraction of SA tremendously decreased as the aqueous pH was increased from 2 to 5. In SLM, initial SA flux was calculated as 9.65 g/m 2 h and doubled that of lactic acid. Selective extraction of only SA was not achieved; however, residue biological material and macromolecular substances were effectively removed. Herein, we clearly demonstrated that process integration applied in reactive extraction is a promising approach for recovery of SA from fermentation broth.
Production of succinic acid by bacterial fermentation
Enzyme and Microbial Technology, 2006
Succinic acid produced by various microorganisms can be used as a precursor of many industrially important chemicals in food, chemical and pharmaceutical industries. The assessment of raw material cost and the estimation of the potential market size clearly indicate that the current petroleum-based succinic acid process will be replaced by the fermentative succinic acid production system in the foreseeable future. This paper reviews processes for fermentative succinic acid production, especially focusing on the use of several promising succinic acid producers including Actinobacillus succinogenes, Anaerobiospirillum succiniciproducens, Mannheimia succiniciproducens and recombinant Escherichia coli. Processes for the recovery of succinic acid from fermentation broth are also reviewed briefly. Finally, we suggest further works required to improve the strain performance suitable for successful commercialization of fermentative succinic acid production.