Antidiabetic activity of an ethanol extract obtained from the stem bark of Psidium guajava (Myrtaceae) (original) (raw)
Related papers
Delta Medical College Journal, 2017
Background: Diabetes Mellitus is a major health problem not only in urban but also in the rural areas of Bangladesh. Regarding its treatment, a suitable drug is yet to be available which can permanently cure this disease. Over 400 traditional plants have been reported for the treatment of diabetes, but only a small number have received scientific and medical evaluation to assess their efficacy. Objective: The study was performed to compare the anti diabetic effect of Psidium guajava Linn leaves with an oral anti diabetic drug (Glibenclamide), in experimentally induced diabetic rats.
pharmacologyonline.silae.it
The antihyperglycaemic activity of the ethanol extract of Psidium guajava (EEPG) leaves was studied by administering three doses of EEPG (i. e.100,200 and 400 mg/kg, p. o.) to alloxan (70 mg/kg, i. v.) induced diabetic mice. The serum glucose levels and body weights of mice were determined. The acute oral toxicity study showed no mortality upto 5000 mg/kg p. o. dose of EEPG. In acute study the antihyperglycaemic effect was observed at 2h (78.25 mg/dl), peak at 6 h (197.03 mg/dl) but antihyperglycaemic effect vanished at 24 h. The subacute study was also carried out which showed maximum reduction in serum glucose level (311.22 mg/dl) at the dose of 200 mg/kg on 28 th day. The oral glucose tolerance test (OGTT) was carried out after administration of EEPG in non-diabetic and diabetic mice previously loaded with (2.5 g/kg, p. o.) glucose. EEPG (200 mg/kg) showed increased glucose threshold in non-diabetic mice. EEPG (800 mg/kg) showed increased glucose threshold in diabetic mice. EEPG (200 mg/kg) prevented the loss of body weight. These results indicated antihyperglycaemic activity of EEPG (200 mg/kg) in alloxan induced diabetic mice. The antihyperglycaemic activity of EEPG was comparable with glyburide.
Effect of Ethanolic Leaf Extract of Psidium guajava Linn. (Guava) in Alloxan-Induced Diabetic Rats.
British Journal of Pharmaceutical Research., 2017
Effect of water – soluble components of ethanolic leaf extract of Psidium guajava Linn. on blood glucose and cholesterol levels in alloxan-induced diabetic male albino wistar rats was evaluated. A total of 30 male albino wistar rats were randomized into 4 groups. Ten (10) animals each were randomly assigned to groups 1 and 2 and 5 each for groups 3 and 4. Group 1 animals were induced with 150 mg of alloxan/kg body weight of animal, after which they were administered 400 mg of extracts/kg body weight of animal. Group 2 animals serving as diabetic control were administered only 150 mg of alloxan/kg body weight of animal, no extract was given. Group 3 animals received only 400 mg of extract/kg body weight of animal while Group 4 animals served as normal control and were given distilled water in place of the extract. Extracts were administered twice daily and treatments lasted for 10 consecutive days. Blood samples were collected for analysis every two days from the tail tips of the rats. Results obtained showed that there were significant (p < 0.05) reductions in blood glucose and cholesterol levels in the diabetic treated rats (Group 1) when compared with the diabetic control rats (Group 2). However, administration of the extract did not show any hypoglycaemic effect on the normal rats (p ˃ 0.05) compared with the control. The results suggest that leaf extract of P. guajava Linn. is antihyperglycaemic and not hypoglycaemic. In view of the fact that it also showed significant antihypercholesterolaemia, P. guajava Linn. may be a potential antidiabetic agent.
Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To evaluate the antidiabetic and the antidiarrhoeal effects of ethanolic extracts of Psidium guajava leave (EEPGL) in Wister rats to support its traditional uses. Methods: Oral glucose tolerance test model and alloxan induced diabetic test model were performed to evaluate antidiabetic activity of EEPGL at doses of 1.00, 0.50 and 0.75 g/kg respectively. For antidiarrhoeal effects of EEPGL, castor oil-induced diarrhoea model and gastrointestinal motility test with barium sulphate milk model were also assessed at doses of 750, 500 and 250 mg/kg, respectively. Results: Administration of EEPGL at doses 1.00 and 0.50 g/kg significantly (P<0.05) decreased blood glucose levels in oral glucose tolerance test model as well as 0.75 g/kg dose in alloxan induced diabetic test model in Wister rats (P<0.001). Application of EEPGL at doses of 750 and 500 mg/kg showed antidiarrhoeal effect in castor oil-induced diarrhoeal model (P<0.001 and P<0.01, respectively), and 750 mg/kg (P<0.01), 500 and 250 mg/kg (P<0.05) doses in barium sulphate milk model in aforesaid animals. Conclusions: These results exhibited the significant antidiabetic and antidiarrhoeal activities of ethanolic extracts of Psidium guajava leave in Wister rats
Asian Pacific Journal of Tropical Biomedicine, 2015
Objective: To evaluate the antidiabetic and the antidiarrhoeal effects of ethanolic extracts of Psidium guajava leave (EEPGL) in Wister rats to support its traditional uses. Methods: Oral glucose tolerance test model and alloxan induced diabetic test model were performed to evaluate antidiabetic activity of EEPGL at doses of 1.00, 0.50 and 0.75 g/kg respectively. For antidiarrhoeal effects of EEPGL, castor oil-induced diarrhoea model and gastrointestinal motility test with barium sulphate milk model were also assessed at doses of 750, 500 and 250 mg/kg, respectively. Results: Administration of EEPGL at doses 1.00 and 0.50 g/kg significantly (P<0.05) decreased blood glucose levels in oral glucose tolerance test model as well as 0.75 g/kg dose in alloxan induced diabetic test model in Wister rats (P<0.001). Application of EEPGL at doses of 750 and 500 mg/kg showed antidiarrhoeal effect in castor oil-induced diarrhoeal model (P<0.001 and P<0.01, respectively), and 750 mg/kg (P<0.01), 500 and 250 mg/kg (P<0.05) doses in barium sulphate milk model in aforesaid animals. Conclusions: These results exhibited the significant antidiabetic and antidiarrhoeal activities of ethanolic extracts of Psidium guajava leave in Wister rats.
Anti-hyperglycemic Activity of Psidium guajava Bark Extract
Journal of Natural Remedies, 2004
Objective: To evaluate the anti-hyperglycemic activity of water extract of Psidium guajava stem bark. Materials and methods: The water extract of stem bark of Psidium guajava was tested for its hypoglycemic activity in normal glucose loaded and alloxan-induced hyperglycemic Wistar rats. Blood glucose levels were evaluated at intervals of 30 and 90 minutes in normal glucose loaded animals, at intervals of 0, 1 and 3 h in acute study and at days 1, 3, 7 and 10 during sub acute treatment after extract administration at an oral dose of 250 mg/kg. Results: During both acute and sub-acute tests, the water extract showed statistically significant and considerable anti-hyperglycemic activity and enhanced glucose tolerance in normal glucose loaded rats. Conclusion: Psidium guajava stem bark possessed statistically significant anti-hyperglycemic potential in alloxan induced diabetic rats and enhanced glucose tolerance in glucose loaded normal rats.
Over the past decade, herbal medicine have been accepted universally and they have an impact on both world health and international trade. Diabetes mellitus is a metabolic disorder in the endocrine system. Plants provide a potential source of hypoglycemic drugs and are widely used in several traditional systems of medicine to prevent diabetes.Qualitative phytochemical analysis of aqueous extract of flower of psidium guajava was carried out with a view of developing leads for a new therapeutic products. The study was designed to investigate the hypoglycaemic effects of oral administration of aqueous extracts of psidium guajava flower in alloxan induced diabetic rats. After oral administration of the flower extract to diabetic rats, the blood glucose level significantly reduced, which is much faster and more than that of metformin. The aqueous extracts of flower of psidium guajava significantly reduced plasma glucose, creatinine, urea, AST, ALT, ACP, ALP, LPO, glucose-6- phosphatase and fructose1,6 bisphosphatase but increased level of serum insulin, protein, CAT, SOD, glycogen synthase and hexokinase. For all the above biochemical parameters observed and it was reverted toits normal level after flower extract treatment. The present investigation suggests that flowers of psidium guajava exhibit hypoglycaemic activity in alloxan induced diabetes in experimental rats.
2021
In Nigeria, rural inhabitants often resort to herbal remedies and dietary control for the treatment and management of various forms of diabetes mellitus. This study was conducted to provide the rationale for the use of Psidium guajava leaves as a potent traditional antidiabetic remedy. The crude leaf extracts of n-hexane, methanol, and ethyl acetate of Psidium guajava were separately prepared by cold maceration. Then, the ethyl acetate crude extract of Psidium guajava leaves was fractionated by column chromatography to yield ethyl acetate bulked fractions: EF-I (1–75), EF-II (76–150), and EF- III (151–250). The n-hexane, ethyl acetate, and methanol crude leaf extracts and ethyl acetate bulked fractions (EF-I, EF-II, and EF-III) were evaluated for antidiabetic activity in alloxan-induced diabetic mice. The blood sugar levels of treated and untreated alloxan-induced diabetic mice were assayed as indices of antidiabetic effect. The phytochemical constituents of both crude extracts and ...
Background: Psidium guajava L (Guava) belongs to the Myrtaceae family and has been claimed to possess several pharmacological properties including antidiabetic. Objective: This study was designed to evaluate the anti-hyperglycemic activity of P guajava L leaves aqueous extract on neonatal streptozotocin-induced type 2 diabetic model rats. Methods: Streptozotocin was induced (90 mg/kg) intraperitoneally to 48 h old Long Evans rat pups. After three months, 18 male type-2 diabetic model rats were confirmed by OGTT (FG > 7 mmol/L). Therefore, experimental rats were divided into three groups 2) Diabetic water control (10 ml/kg), 3) Gliclazide treated (20 mg/kg), and 4) Extract treated group (1.25g/kg)] Six normal female rats comprised group 1 [Non-diabetic water control (10 ml/kg)]. All rats were treated orally with their respective treatment for 28 consecutive days. Blood samples were collected on 0 days (by tail cut method) and the end day (by cardiac puncture) of the experiment. The anti-hyperglycemic activity was evaluated by measuring fasting glucose, serum insulin, lipid profile, hepatic glycogen content, and intestinal glucose absorption by standard methods. Results: The serum glucose level of extract treated group was decreased by 16% as well as significantly (p<0.05) increased the serum insulin level (M±SD, 0 day vs 28thday; 0.319 ± 0.110 vs 0.600 ± 0.348, mg/L). Moreover, the extract-treated group also significantly (p<0.05) enhanced liver glycogen content and inhibited glucose absorption from the upper intestine. Besides, a significant (p < 0.05) reduction of LDLcholesterol level was found in the extract-treated group (M±SD, 55 ± 33 vs 14 ± 9, mg/dl) compared with baseline values where other groups did not show any statistically remarkable changes. Conclusion: Current study concludes that P guajava leaves aqueous extract enhances insulin secretion from pancreatic beta-cells and promotes glycogen synthesis in the liver. The extract also inhibits glucose absorption from the upper intestine and improves dyslipidemia to some extent. Therefore, possesses the potential for drug development against T2DM.
Hypoglycemic Activity of Psidium guajava Linn. Leaf Extract
Journal of Natural Remedies, 2004
Objective: To evaluate the hypoglycemic activity of ethanol extract of Psidium guajava leaves. Materials and methods: The ethanol extract of leaves of Psidium guajava was tested for its hypoglycemic activity in alloxan-induced hyperglycemic Wistar rats. Results: In both acute and sub-acute tests, the ethanol extract, at an oral dose of 250 mg/kg, showed statistically significant and considerable hypoglycemic activity. Conclusion: Psidium guajava leaves possess statistically significant hypoglycemic activity.