Comparative Study of the Effect of Ethanol Extract of Psidium Guajava Linn Leaves with Glibenclamide on Experimentally Induced Diabetes Mellitus in Rats (original) (raw)
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2021
In Nigeria, rural inhabitants often resort to herbal remedies and dietary control for the treatment and management of various forms of diabetes mellitus. This study was conducted to provide the rationale for the use of Psidium guajava leaves as a potent traditional antidiabetic remedy. The crude leaf extracts of n-hexane, methanol, and ethyl acetate of Psidium guajava were separately prepared by cold maceration. Then, the ethyl acetate crude extract of Psidium guajava leaves was fractionated by column chromatography to yield ethyl acetate bulked fractions: EF-I (1–75), EF-II (76–150), and EF- III (151–250). The n-hexane, ethyl acetate, and methanol crude leaf extracts and ethyl acetate bulked fractions (EF-I, EF-II, and EF-III) were evaluated for antidiabetic activity in alloxan-induced diabetic mice. The blood sugar levels of treated and untreated alloxan-induced diabetic mice were assayed as indices of antidiabetic effect. The phytochemical constituents of both crude extracts and ...
Journal of Basic Medical Veterinary, 2024
Background:The chronic disease known as diabetes mellitus is brought on by either the pancreas's inability to make enough insulin or the body's inability to use it. Purpose:This plant finds applications for treating diarrhea, dysentery, gastroenteritis, hypertension, diabetes, caries and pain relief. Purpose:The current study aimed to determine how Psidium guajavafruit extract affected the blood glucose, body weights, and insulin levels of streptozotocin (STZ)-induced diabetic rats over 21 days. Methods:The extract's effectiveness was compared to that of glibenclamide, a common hypoglycemic medication. 30 male Wistar albino rats were divided into 5 groups with 6 animals in each group. V: Normal control (Group-I), diabetic control (Group-II), diabetic rats treated with glibenclamide 0.6mg/kg bw (Group-III), diabetic rats treated with Psidium guajavafruit extract 200 mg/kg bw (Group-IV) and Psidium guajavafruit extract 400 mg/kg bw (Group-V). All group of rats were subjected to evaluation of body weight, blood glucose and serum insulin levels on day 0, 7, 14 and 21 of the experiment. Results:There was significant (P<0.05) decrease in body weight and serum insulin and significant (P<0.05) increase in blood glucose level in Group-II compared to Group-I rats. In the Present study, daily oral administration of Psidium guajavafruit extract at dose rate of 200 and 400 mg/kg bw and glibenclamide at 0.6mg/kg bw in diabetic rats for 21 days showed a progressive improvement in body weight, blood glucose and serum insulin concentration. Conclusion:It can therefore be concluded the results of this study indicate that Psidium guajavafruit extract possesses anti-diabetic properties in Wistar albino rats
Over the past decade, herbal medicine have been accepted universally and they have an impact on both world health and international trade. Diabetes mellitus is a metabolic disorder in the endocrine system. Plants provide a potential source of hypoglycemic drugs and are widely used in several traditional systems of medicine to prevent diabetes.Qualitative phytochemical analysis of aqueous extract of flower of psidium guajava was carried out with a view of developing leads for a new therapeutic products. The study was designed to investigate the hypoglycaemic effects of oral administration of aqueous extracts of psidium guajava flower in alloxan induced diabetic rats. After oral administration of the flower extract to diabetic rats, the blood glucose level significantly reduced, which is much faster and more than that of metformin. The aqueous extracts of flower of psidium guajava significantly reduced plasma glucose, creatinine, urea, AST, ALT, ACP, ALP, LPO, glucose-6- phosphatase and fructose1,6 bisphosphatase but increased level of serum insulin, protein, CAT, SOD, glycogen synthase and hexokinase. For all the above biochemical parameters observed and it was reverted toits normal level after flower extract treatment. The present investigation suggests that flowers of psidium guajava exhibit hypoglycaemic activity in alloxan induced diabetes in experimental rats.
Effect of Ethanolic Leaf Extract of Psidium guajava Linn. (Guava) in Alloxan-Induced Diabetic Rats.
British Journal of Pharmaceutical Research., 2017
Effect of water – soluble components of ethanolic leaf extract of Psidium guajava Linn. on blood glucose and cholesterol levels in alloxan-induced diabetic male albino wistar rats was evaluated. A total of 30 male albino wistar rats were randomized into 4 groups. Ten (10) animals each were randomly assigned to groups 1 and 2 and 5 each for groups 3 and 4. Group 1 animals were induced with 150 mg of alloxan/kg body weight of animal, after which they were administered 400 mg of extracts/kg body weight of animal. Group 2 animals serving as diabetic control were administered only 150 mg of alloxan/kg body weight of animal, no extract was given. Group 3 animals received only 400 mg of extract/kg body weight of animal while Group 4 animals served as normal control and were given distilled water in place of the extract. Extracts were administered twice daily and treatments lasted for 10 consecutive days. Blood samples were collected for analysis every two days from the tail tips of the rats. Results obtained showed that there were significant (p < 0.05) reductions in blood glucose and cholesterol levels in the diabetic treated rats (Group 1) when compared with the diabetic control rats (Group 2). However, administration of the extract did not show any hypoglycaemic effect on the normal rats (p ˃ 0.05) compared with the control. The results suggest that leaf extract of P. guajava Linn. is antihyperglycaemic and not hypoglycaemic. In view of the fact that it also showed significant antihypercholesterolaemia, P. guajava Linn. may be a potential antidiabetic agent.
Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To evaluate the antidiabetic and the antidiarrhoeal effects of ethanolic extracts of Psidium guajava leave (EEPGL) in Wister rats to support its traditional uses. Methods: Oral glucose tolerance test model and alloxan induced diabetic test model were performed to evaluate antidiabetic activity of EEPGL at doses of 1.00, 0.50 and 0.75 g/kg respectively. For antidiarrhoeal effects of EEPGL, castor oil-induced diarrhoea model and gastrointestinal motility test with barium sulphate milk model were also assessed at doses of 750, 500 and 250 mg/kg, respectively. Results: Administration of EEPGL at doses 1.00 and 0.50 g/kg significantly (P<0.05) decreased blood glucose levels in oral glucose tolerance test model as well as 0.75 g/kg dose in alloxan induced diabetic test model in Wister rats (P<0.001). Application of EEPGL at doses of 750 and 500 mg/kg showed antidiarrhoeal effect in castor oil-induced diarrhoeal model (P<0.001 and P<0.01, respectively), and 750 mg/kg (P<0.01), 500 and 250 mg/kg (P<0.05) doses in barium sulphate milk model in aforesaid animals. Conclusions: These results exhibited the significant antidiabetic and antidiarrhoeal activities of ethanolic extracts of Psidium guajava leave in Wister rats
Asian Pacific Journal of Tropical Biomedicine, 2015
Objective: To evaluate the antidiabetic and the antidiarrhoeal effects of ethanolic extracts of Psidium guajava leave (EEPGL) in Wister rats to support its traditional uses. Methods: Oral glucose tolerance test model and alloxan induced diabetic test model were performed to evaluate antidiabetic activity of EEPGL at doses of 1.00, 0.50 and 0.75 g/kg respectively. For antidiarrhoeal effects of EEPGL, castor oil-induced diarrhoea model and gastrointestinal motility test with barium sulphate milk model were also assessed at doses of 750, 500 and 250 mg/kg, respectively. Results: Administration of EEPGL at doses 1.00 and 0.50 g/kg significantly (P<0.05) decreased blood glucose levels in oral glucose tolerance test model as well as 0.75 g/kg dose in alloxan induced diabetic test model in Wister rats (P<0.001). Application of EEPGL at doses of 750 and 500 mg/kg showed antidiarrhoeal effect in castor oil-induced diarrhoeal model (P<0.001 and P<0.01, respectively), and 750 mg/kg (P<0.01), 500 and 250 mg/kg (P<0.05) doses in barium sulphate milk model in aforesaid animals. Conclusions: These results exhibited the significant antidiabetic and antidiarrhoeal activities of ethanolic extracts of Psidium guajava leave in Wister rats.
Pharmazie
This study analyzed the antidiabetic properties of an ethanol extract of the stem bark of Psidium guajava, an indigenous medicinal plant used to control diabetes in Indian System of Medicine. The anti-hyperglycaemic activity of this plant on blood glucose levels of normal, normal glucose loaded (OGTT) and alloxan-induced hyperglycaemic rats was evaluated. The results showed that ethanol stem bark extract exhibited statistically significant hypoglycaemic activity in alloxan-induced hyperglycaemic rats but was devoid of significant hypoglycaemic effect in normal and normal glucose loaded rats (OGTT).
Background: Psidium guajava L (Guava) belongs to the Myrtaceae family and has been claimed to possess several pharmacological properties including antidiabetic. Objective: This study was designed to evaluate the anti-hyperglycemic activity of P guajava L leaves aqueous extract on neonatal streptozotocin-induced type 2 diabetic model rats. Methods: Streptozotocin was induced (90 mg/kg) intraperitoneally to 48 h old Long Evans rat pups. After three months, 18 male type-2 diabetic model rats were confirmed by OGTT (FG > 7 mmol/L). Therefore, experimental rats were divided into three groups 2) Diabetic water control (10 ml/kg), 3) Gliclazide treated (20 mg/kg), and 4) Extract treated group (1.25g/kg)] Six normal female rats comprised group 1 [Non-diabetic water control (10 ml/kg)]. All rats were treated orally with their respective treatment for 28 consecutive days. Blood samples were collected on 0 days (by tail cut method) and the end day (by cardiac puncture) of the experiment. The anti-hyperglycemic activity was evaluated by measuring fasting glucose, serum insulin, lipid profile, hepatic glycogen content, and intestinal glucose absorption by standard methods. Results: The serum glucose level of extract treated group was decreased by 16% as well as significantly (p<0.05) increased the serum insulin level (M±SD, 0 day vs 28thday; 0.319 ± 0.110 vs 0.600 ± 0.348, mg/L). Moreover, the extract-treated group also significantly (p<0.05) enhanced liver glycogen content and inhibited glucose absorption from the upper intestine. Besides, a significant (p < 0.05) reduction of LDLcholesterol level was found in the extract-treated group (M±SD, 55 ± 33 vs 14 ± 9, mg/dl) compared with baseline values where other groups did not show any statistically remarkable changes. Conclusion: Current study concludes that P guajava leaves aqueous extract enhances insulin secretion from pancreatic beta-cells and promotes glycogen synthesis in the liver. The extract also inhibits glucose absorption from the upper intestine and improves dyslipidemia to some extent. Therefore, possesses the potential for drug development against T2DM.
The Egyptian Journal of Hospital Medicine
The present aim is to evaluate the anti-diabetic effect of aqueous extract of guava (Psidium guajava) leaf using rat model of diabetes induced by streptozotocin. In addition, the effect of this extract on liver and kidney functions induced in rat model of diabetes were investigated. Material and Methods: Rats were divided randomly into: control group, rat model of diabetes induced by streptozotocin, rat model of diabetes treated with aqueous extract of guava leaf, rat model of diabetes treated with glibenclamide and rat model of diabetes treated with aqueous extract of guava leaf plus glibenclamide. Result: In the present rat model of diabetes a significant decrease in the serum insulin level and a significant increase in glucose level were detected. Streptozotocin induced a significant increase in the activities of AST, ALT, ALP and a significant increase in the levels of bilirubin, urea, creatinine and uric acid. In addition histopathological and immunological changes were detected in the pancreatic tissue. The present data revealed that aqueous extract of guava leaf improved the reduced insulin level and the high glucose level induced by streptozotocin. This was associated with an improvement in the changes in the liver and kidney functions. Loss of body weight gain induced by streptozotocin was alleviated by guava leaf extract, glibenclamide or both. Conclusion: According to the present findings it could be concluded that the aqueous extract of guava leaf has a potent anti-hyperglycemic effect on rat model of diabetes induced by streptozotocin with hepatic and renal protective effects.
pharmacologyonline.silae.it
The antihyperglycaemic activity of the ethanol extract of Psidium guajava (EEPG) leaves was studied by administering three doses of EEPG (i. e.100,200 and 400 mg/kg, p. o.) to alloxan (70 mg/kg, i. v.) induced diabetic mice. The serum glucose levels and body weights of mice were determined. The acute oral toxicity study showed no mortality upto 5000 mg/kg p. o. dose of EEPG. In acute study the antihyperglycaemic effect was observed at 2h (78.25 mg/dl), peak at 6 h (197.03 mg/dl) but antihyperglycaemic effect vanished at 24 h. The subacute study was also carried out which showed maximum reduction in serum glucose level (311.22 mg/dl) at the dose of 200 mg/kg on 28 th day. The oral glucose tolerance test (OGTT) was carried out after administration of EEPG in non-diabetic and diabetic mice previously loaded with (2.5 g/kg, p. o.) glucose. EEPG (200 mg/kg) showed increased glucose threshold in non-diabetic mice. EEPG (800 mg/kg) showed increased glucose threshold in diabetic mice. EEPG (200 mg/kg) prevented the loss of body weight. These results indicated antihyperglycaemic activity of EEPG (200 mg/kg) in alloxan induced diabetic mice. The antihyperglycaemic activity of EEPG was comparable with glyburide.