Phytochemical screening and bioactivity studies of Phyllanthus wightianus (original) (raw)
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Antioxidant activities of the standardized water extract from fruit of phyllanthus emblica linn
Songklanakarin Journal of Science and Technology, 2010
Phyllanthus emblica Linn. is widely used in Thai traditional medicine for treatment of various diseases. The fruit of P. emblica is known as a rich source of vitamin C, and also contains a mixture of phenolic compounds. In this study, the standardized water extract of P. emblica fruit was prepared according to Thai Herbal Pharmacopoeia. Total polyphenol contents of the extract were equivalent to 34.22±1.74 g gallic acid/100g extract. Antioxidant activities of the P. emblica extract were evaluated by several methods, including DPPH and ABTS •+ radical scavenging assays and FRAP assays. The results showed that the extract has an ability of scavenging radicals generated by both DPPH and ABTS •+ . Similar to Trolox, the water extract of P. emblica fruit also had a ferric reducing property. Additionally, the extract effectively inhibited H 2 O 2 - induced free radical production in human myeloleukemic U937 cells as measured by 2,7-DCF-DA. The results imply that the fruits of P. emblica a...
Antioxidant Studies of Aqueous Extract of Phyllanthus Reticulatus Poir
2008
Summary Free radicals are implicated for many diseases including Diabetes mellitus, arthritis, cancer, ageing. etc. In treatment of these diseases, antioxidant therapy has gained utmost importance. Phyllanthus reticulatus Poir (Euphrobiaceae) popularly known as ‘potatobush’ is an important medicinal plant and useful in burning sensation, strangury, gastropathy, ophthalmodynia, burns, diarrhoea, skin eruption, diabetes and obesity. Keeping in view of the cited activity, it is contemplated to screen the plant for in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, iron chelating activity and lipid peroxidation assay, nitric oxide scavenging assay, alkaline DMSO assay, total antioxidant capacity and non-enzymatic haemoglobin glycosylation assay . The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 20.36 µgm/ml (DPPH radical scavenging), 42.59 µgm/ml ...
Evaluation of in Vitro Anti-Inflammatory Activity and HPTLC Analysis of Plant Phyllanthus Fraternus
International Journal of Current Pharmaceutical Research
Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.Â
IN VITRO ANTIOXIDANT ACTIVITIES OF PHYLLANTHUSAMARUS HERBS
In this study, measure antioxidant potential of P. amarus by DPPH radical scavenging activity, Superoxide radical scavenging activity and Hydroxyl radical scavenging activity method using ether, aqueous and ethanol extract in different propagation also reference compound as Ascorbic Acid. In DPPH radical scavenging activity, ethanol extract show higher percentage inhibition 93.50% at 250µg/mL. Higher percentage inhibition of all three activities i.e. DPPH, SOD and hydroxyl at 250 µg/mL by ethanol extract is 96.50 % with reference compound i.e. Ascorbic Acid-97.00%.
A Study on Phytochemical Analysis of Phyllanthus Tenellus Roxb
International Research Journal of Pharmacy
Phyllanthus tenellus is a dominant plant in herbaceous family to appear frequently in various humid environments in Warangal region in Telangana. This plant is used to treat different disease like urolithiasis, inflammatory bowel disease, diabetes and hepatitis B. The present study developed phylogenetic relationship with secondary metabolites and other related compounds of 2 other species of Phyllanthus belongs to family Euphorbiaceae. This investigation was to establish the phylogenetic relationship with secondary metabolites and other related compounds of Phyllanthus tenellus of Euphorbiaceae family. Among them, Phyllanthus tenellus shows unique relationship with secondary metabolites, protein related compound.
Phytochemical analysis, antioxidant and anti-inflammatory activities of Phyllanthus simplex
Journal of Ethnopharmacology, 2011
Ethnopharmacological relevance: Phyllanthus simplex (Family: Euphorbiacae) is widely used in traditional medicines for treatment of various diseases including inflammation. Materials and methods: Petroleum ether extract (PSPE) and ethanol extract (PSEE) of the whole plant of Phyllanthus simplex were characterized for their total phenolics, tannins and flavonoids content. These extracts were standardized by HPTLC using phyllanthin and gallic acid respectively as markers. Antioxidant activity of extracts was evaluated by the DPPH, hydroxyl and superoxide radicals scavenging assay. The total antioxidant capacity of extracts was determined. Anti-inflammatory activity was evaluated by their effect on nitric oxide (NO) production in isolated rat peritoneal macrophages; carragennan-induced paw edema and formation of cotton pellet-induced granuloma in rats. Results: Abundance of phenolics was found in PSEE. Phyllanthin and gallic acid content in PSPE and PSEE were found to be 14.5 and 0.65% (w/w) respectively. PSEE showed concentration dependent significant scavenging of DPPH, hydroxyl and superoxide radicals with IC 50 values 102.219, 171.485 and 24.73 g/ml respectively. PSEE significantly inhibited NO production in isolated rat peritoneum macrophages. Moreover, it also exhibited significant inhibition of carragennan-induced paw edema (58.48 ± 0.028%, p < 0.001, at 6 h, 200 mg/kg oral dose) and cotton pellet-induced granuloma formation (45.671 ± 0.712%, p < 0.001, at 200 mg/kg oral dose). Anti-inflammatory activity of PSEE was found to be comparable to diclofenac sodium. Conclusions: Significant antioxidant and anti-inflammatory activities were found in PSEE which may be attributed to its high phenolic content.
Anti-inflammatory activities of constituents isolated from Phyllanthus polyphyllus
2006
Four compounds, including one benzenoid, 4-O-methylgallic acid (1), together with three arylnaphthalide lignans, namely phyllamyricin C (2), justicidin B (3) and diphyllin (4) were isolated from the whole plants of Phyllanthus polyphyllus L. (Euphorbiaceae). This was the first isolation report of compounds 1-4 from this plant species. The in vitro inhibitory effects of these compounds were evaluated on the production of nitric oxide (NO) and cytokines (tumor necrosis factor (TNF)-␣ and interleukin (IL)-12), from LPS/IFN-␥ activated murine peritoneal macrophages. The results indicated that the 50% inhibition concentration (IC 50) values of NO production from activated peritoneal macrophages by compounds 1-4 were 100, 25, 12.5 and 50 M, respectively. In parallel, these dilutions were approximately inhibited in a similar manner to that observed for cytokines (TNF-␣, and IL-12) production. On the other hand, at 100 M concentration compounds 3 and 4 showed 50% inhibition of NO production from peritoneal macrophages that had been pre-activated with LPS/IFN-␥ for 24 h, whereas compounds 1 and 2 inhibited only about 20 and 10%, respectively. These results support the use of this plant for the treatment of inflammatory diseases in oriental traditional medicine.
Background and Objective: All organisms have an antioxidant defense mechanism that uses enzymes like superoxide dismutase and catalase, as well as substances like ascorbic acid, tocopherol and glutathione, to protect them from free radical damage. Consumption of more antioxidants (phytochemicals) from herbs like Phyllanthus amarus, meals or supplements boosts the body's antioxidant defense mechanism, protecting it against toxic ROS. The goal of this study was to see if there are any antioxidants in the ethanolic extract fractions of Phyllanthus amarus leaves. Materials and Methods: The leaves of Phyllanthus amarus were taken from the Federal University Wukari Campus in Taraba State, Nigeria, dried and crushed before being subjected to anti-lipid peroxidation inhibition assay, β-carotene bleaching inhibition assay, total phenolic content determination, total flavonoid content determination and total antioxidant capacity determination further tests. Results: According to the findings of this study, fraction 4b (445.9459%) has the maximum bleaching inhibition power for beta-carotene inhibition testing, whereas, fraction 4a (81.08109%) has the lowest. Fraction 2a (56.9565 mg mLG 1) has the highest phenolic content while fraction 6b (7.608669 mg mLG 1) has the lowest phenolic content. The assay for total flavonoid content showed that fraction 3a (145.71428 mg mLG 1) has the highest flavonoid content while fraction 8a (121.42857 mg mLG 1) has the lowest flavonoid content. The results for anti-lipid peroxidation revealed that fraction 3b (55.732 mg mLG 1) has the highest antioxidant capacity while fraction 6a (2.775 mg mLG 1) has the lowest antioxidant activity. The results of the analysis revealed that Phyllanthus amarus leaves contain significant nutritional components that are good for human health. Conclusion: It is reasonable to conclude that fractions 2a and 2b have the highest antioxidant activities which have good potential for drug development, especially against diseases of oxidative stress origin.
2012
Stem methanolic extract of Phyllanthus serpentinicola was screened for phytochemicals, and tested for cytotoxicity and anti-inflammatory activities. Tests indicated presence of alkaloids, tannins, terpenoids, reducing sugars and flavonoids. Saponins, anthraquinones, and cardiac glycosides were absent. Cytotoxic effects of the plant were assessed through Brine shrimp lethality (BSL) bioassay. An LC 50 value of 1.3 µg/ml was found, thus indicating that the stem extract was strongly cytotoxic. Anti-inflammatory activity was assessed by the egg albumen induced hind paw oedema test on Swiss albino mice. The extract significantly (p<0.05) reduced the oedema, thus indicating that P. serpentinicola has anti-inflammatory properties.
2015
The aqueous extract of Phyllanthus orbicularis contains bioproducts able to significantly reduce the mutagenesis mediated by oxidative compounds. A bioactive-directed fractionation of the aqueous extract of P. orbicularis has been applied with the aim of identifying antimutagenic fractions, which allowed isolating and identifying some biocompound responsible for this activity. Antimutagenesis of all fractions was determined by the Salmonella retromutation assay, using hydrogen peroxide as model mutagen. Applying this methodology, two potent antimutagenic fractions have been obtained able to totally abolish the mutagenicity produced by hydrogen peroxide. In these fractions 2,4-di-tertbutylphenol, OO’-diphenol-4,4’,6,6’-tetra-tert-butyl and 2,6-di-sec-butylphenol were identified by Gas Chromatography/Mass Spectrometry. Keywords: P. orbicularis; 2,4-di-tert-butylphenol; OO’-diphenol-4,4’,6,6’-tetra-tert-butyl; 2,6-di-secbutylphenol; antimutagenesis; oxidative damage; chemical fractiona...