The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance
Sergei Borukhov
Proceedings of the National Academy of Sciences, 2000
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Equilibrium and Stopped-flow Kinetic Studies of Interaction between T7 RNA Polymerase and Its Promoters Measured by Protein and 2-Aminopurine Fluorescence Changes
Amar Kant Kumar
Journal of Biological Chemistry, 1996
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Initiation Complexes of Escherichia coli RNA Polymerase and T7 DNA
David McConnell
European Journal of Biochemistry, 1973
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Stability of ternary transcription complexes of vaccinia virus RNA polymerase at promoter-proximal positions
James Hagler
Journal of Biological Chemistry, 1992
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Preventing nondesired RNA-primed RNA extension catalyzed by T7 RNA polymerase
Alfredo Berzal-herranz, Genoveva Nacheva
European Journal of Biochemistry, 2003
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A Critical Residue Selectively Recruits Nucleotides for T7 RNA Polymerase Transcription Fidelity Control
Jin Yu
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Real-time characterization of intermediates in the pathway to open complex formation by Escherichia coil RNA polymerase at the T7AI promoter
bianca sclavi
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Moieties in an RNA promoter specifically recognized by a viral RNA-dependent RNA polymerase
Laurent Bellon
Proceedings of the National Academy of Sciences, 1998
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Evidence for DNA bending at the T7 RNA polymerase promoter
Andrea Újvári
Journal of Molecular Biology, 2000
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Alteration of the −35 and −10 sequences and deletion the upstream sequence of the −35 region of the promoter A1 of the phage T7 in dsDNA confirm the contribution of non-specific interactions with E. coli RNA polymerase to the transcription initiation process
Katarzyna Turecka
Frontiers in Molecular Biosciences, 2024
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Structure and Function in Promoter Escape by T7 RNA Polymerase
karsten theis
Progress in Nucleic Acid Research and Molecular Biology, 2005
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Study of elongation complexes for T7 RNA polymerase
Gimena Ramos
Biophysics, 2012
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DNA melting by RNA polymerase at the T7A1 promoter precedes the rate-limiting step at 37 C and results in the accumulation of an off-pathway intermediate
Hermann Heumann
Nucleic Acids Research, 2009
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Pre-Steady-State and Steady-State Kinetic Studies on Transcription Initiation Catalyzed by T7 RNA Polymerase and Its Active-Site Mutants K631R and Y639F †
Robert Wody
Biochemistry, 1998
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Structure-Function Relationships of the Viral RNA-dependent RNA Polymerase: FIDELITY, REPLICATION SPEED, AND INITIATION MECHANISM DETERMINED BY A RESIDUE IN THE RIBOSE-BINDING POCKET
Victoria Korneeva
Journal of Biological Chemistry, 2007
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Transcription reinitiation properties of bacteriophage T7 RNA polymerase
Roberto Victor Ferrari
Biochemical and Biophysical Research Communications, 2004
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Identification of a minimal binding element within the T7 RNA polymerase promoter
Andrea Újvári
Journal of Molecular Biology, 1997
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A mutant T7 RNA polymerase as a DNA polymerase
robert Padilla
The EMBO Journal, 1995
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Real-time characterization of intermediates in the pathway to open complex formation by Escherichia coli RNA polymerase at the T7A1 promoter
bianca sclavi
2005
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RNA Polymerase Alters the Mobility of an A-Residue Crucial to Polymerase-Induced Melting of Promoter DNA †
Pieter Dehaseth
Biochemistry, 2002
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Modulation of RNA polymerase activity through the trigger loop folding
Irina Artsimovitch
transcription, 2010
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Importance of Steric Effects on the Efficiency and Fidelity of Transcription by T7 RNA Polymerase
Sébastien Ulrich
Biochemistry, 2011
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From The Cover: Real-time characterization of intermediates in the pathway to open complex formation by Escherichia coli RNA polymerase at the T7A1 promoter
Hermann Heumann
Proceedings of the National Academy of Sciences, 2005
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Functional Architecture of T7 RNA Polymerase Transcription Complexes
Dhananjaya Nayak
Journal of Molecular Biology, 2007
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Triphosphate Re‐orientation of the Incoming Nucleotide as A Fidelity Checkpoint in Viral RNA‐dependent RNA Polymerases
David Boehr
The FASEB Journal, 2015
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The -11A of promoter DNA and two conserved amino acids in the melting region of 70 both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interact
Pieter Dehaseth
Nucleic Acids Research, 2007
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Equilibrium and Kinetic Parameters of the Sequence-Specific Interaction of Escherichia c oli RNA Polymerase with Nontemplate Strand Oligodeoxyribonucleotides †
Pieter Dehaseth, M. Strainic
Biochemistry, 1998
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Real-time footprinting of DNA in the first kinetically significant intermediate in open complex formation by Escherichia coli RNA polymerase
Craig Bingman
Proceedings of the National Academy of Sciences, 2007
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