Green synthesis of silver nanoparticles using Ocimum leaf extract and their characterization (original) (raw)
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Comprehensive Research and Reviews in Chemistry and Pharmacy, 2022
Ocimum gratissimum is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. The study here, expanded this traditional usage with the green biosynthesis of silver nanoparticles achieved using Ocimum gratissimum leaf extract as a reducing and capping agent. The green synthesis of Ag NPs reaction was carried out using 2mL of Ocimum gratissimum leaves extract added to 50mL aqueous solution comprising 85 mg of silver nitrate The effect of temperature on the synthesis of Ag NPs was examined using room temperature (25 0 C) and 60 0 C. The silver nanoparticles were formed in 20 min by stirring at room temperature. In this case, a deep brown color was developed. The successful formation of silver nanoparticles was further confirmed by UV-Vis and SEM analysis. The characteristic peaks of the UV-vis spectrum and SEM confirmed the synthesis of Ag NPs as the UV spectrum revealed that the maximum absorption peak was at absorbance of 0.99 with corresponding wavelength (λ max) at 500 nm and the SEM micrograph of biosynthesized Ag NPs showed relatively face centered cubic structure, well distributed without aggregation and an average size of about 28nm with 10% of Ocimum gratissimum leaf extract in 1.5 mM Ag nitrate concentration. The biosynthesized Ag NPs exhibited potential antibacterial activity against human pathogenic bacteria. The result clearly suggest that the green biosynthesized Ag NPs can constitute an effective antibacterial agent.
Rapid Biological Synthesis of Silver Nanoparticles from Ocimum sanctum and Their Characterization
Journal of Nanoscience, 2017
With development of nanotechnology, the biological synthesis process deals with the synthesis, characterization, and manipulation of materials and further development at nanoscale which is the most cost-effective and eco-friendly and rapid synthesis process as compared to physical and chemical process. In this research silver nanoparticles (AgNPs) were synthesized from silver nitrate (AgNO 3) aqueous solution through eco-friendly plant leaf broth of Ocimum sanctum as reactant as well as capping agent and stabilizer. The formation of AgNPs was monitored by ultraviolet-visible spectrometer (UV-vis) and Fourier transform infrared (FTIR) spectroscopy. X-ray diffraction (XRD) and scanning electronic microscopy (SEM) have been used to characterize the morphology of prepared AgNPs. The peaks in XRD pattern are in good agreement with that of face-centered-cubic (FCC) form of metallic silver. Thermal gravimetric analysis/differential thermal analysis (TGA/DTA) results confirmed the weight loss and the exothermic reaction due to desorption of chemisorbed water. The average grain size of silver nanoparticles is found to be 29 nm. The FTIR results indicated that the leaf broths containing the carboxyl, hydroxyl, and amine groups are mainly involved in fabrication of silver AgNPs and proteins, which have amine groups responsible for stabilizing AgNPs in the solution.
Shanlax International Journal of Arts, Science and Humanities, 2020
Nanoparticles (NPs) are being widely used in different fields; therefore, there is growing interest in the development of a biological and environmental safety method for their production. Now a day’s chemical and physical methods are being used for the development of nanoparticle that is costly, time-consuming, and harmful for the environment. Plant-mediated synthesis of nanoparticles is a “Green chemistry” approach that connects different types of plants with nanotechnology. It has gained much more attention as a reliable, sustainable, and eco-friendly method for synthesizing a wide range of materials/nanomaterials. Plants are called nature’s “chemical factories” therefore, plants and plant extracts are the best options to produce different types of nanoparticles. In this present study, silver nanoparticles were synthesized by using leaf extract of Ocimum sanctum and aqueous silver nitrate solution through a simple and eco-friendly method. Then the developed silver nanoparticles w...
Journal of Nanoparticle …, 2011
Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV-vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV-vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and grampositive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4-30 nm possessing antimicrobial activity suggesting their possible application in medical industry.
A single step environnemental friendly approach is employed to synthesise Silver nanoparticles using Ocimum tenuiflorum L. green and purple (Tulasi) varieties. Synthesised AgNps are analysed by using UV-Visible, FTIR, Scanning Electron Microscope (SEM) and Energy Dispersive X-ray spectroscope(EDX). The formation of nanoparticles was confirmed by colour change and the surface Plasmon absorption band was measured by the UV-Visible spectroscopy .The FTIR peak corresponds to the presence of C-H vibration of the aromatic ring and the stretch vibration of C-O indicates carbonyl group and flavonoids. SEM analysis of the synthesised Silver nanoparticles are clearly distinguishable and the particles are measured <100nm in size. The EDX spectra show the purity of the material. Synthesis is conventional and found to be efficient in terms of reaction time as well as cost effective.
2019
Ocimum gratissimum leaves were used in the synthesis of silver nanoparticles (AgNps) that were non-toxic and also possessed antimicrobial properties against clinically isolated pathogenic strains. Some bioactive agents in the aqueous extract of Ocimum gratissimum leaves (AEOgL) were identified. AgNp synthesis was carried out by incubating the AEOgL and 1 mM AgNO3. The brownish colour obtained upon reduction of silver by the AEOgL was observed. The phytochemicals present are tannins, glycosides, sterols, phenols, alkaloid and terpenoids. There was the presence of a surface plasmon resonance from the UV-visible scan. The synthesized silver nanoparticles were characterized by UV-visible spectrophotometry, Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy/Energy Dispersive Analysis (SEM/EDX) and X-ray diffraction analysis. The SEM/EDX analysis indicated that the morphology of the nanoparticles is of a uniform structure and the size of the AgNp was 20 nm. The A...
Plant extract are very cost effective and eco-friendly, thus can be an economic and efficient alternate for the large-scale synthesis of nanoparticles. The preparation of stable, uniform silver nanoparticles by reduction of silver ions with Emblica officinalis, Terminalia catappa and Eucalyptus hybrida extract is reported in the present paper. It is a simple process of global research interest for obtaining silver nanoparticles in least amount of time. These nanoparticles were characterized with UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy different X- ray (EDX) analysis which revealed that the silver nanoparticles are polydisperse and of different morphologies ranging from 20 to 80 nm in size. XRD results reveal that these nanostructures exhibits a face-centered cubic crystal structure. The UV/Vis spectra absorption peak confirms their production. Pioneering of reliable and eco-friendly process for synthesis of metallic nanoparticles biologically is an important step in the field of application of nanobiotechnology. Thus, these silver nanoparticles (Ag-NPs) may prove as a better candidate for drugs and can potentially eliminate the problem of chemical agents because of their biogenic nature. The indiscriminate use of antibiotics has fueled the development of drug resistance at an alarming rate. To overpower this burning problem, the AgNPs may prove to be a universal solution.
2020
The silver nanoparticles have found prominence in different fields such as medicine, catalysis, nanoelectronics, textile field, pollution and water treatment due to their unique attributes. Applications of silver nanoparticles are increasing rapidly in the medical purpose including drug delivery, treatment, diagnosis, medical device coating. Various chemical and physical methods are use to synthesize the silver nanoparticles (AgNP) conventionally. But these synthesis processes are expensive and also involves side effects. To solve these savior problems by modification in synthesis process for safer and more efficiency, Green Nanotechnology comes to play a very crucial role for synthesis of
Green Synthesis of Silver Nanoparticles Using Alagaw Leaf Extract
Green Synthesis of Silver Nanoparticles Using Alagaw (Premna odorata) Leaf Extract, 2019
Abstract. There is a worldwide interest in silver nanoparticles (AgNPs) synthesize by various chemical reactions for use in applications. Silver nanoparticles have gained significant interest due to their unique optical, antimicrobial, electrical, physical properties and their possible application. However, it is necessary to develop environmental friendly methods for their syntheses. To avoid chemically toxicity, biosynthesis of metal silver nanoparticles is proposed as a cost-effective and environmental friendly alternative. This study aimed to find out whether Alagaw plant can potentially act as a reducing agent for the biosynthesis of silver nanoparticles and whether the concentration of the leaf extract can affect the absorbance spectrum, size and shape of the synthesized silver nanoparticles. The synthesized silver nanoparticles were characterized using the UV-vis spectroscopy for its absorbance spectrum and Transmission Electron Microscope Analysis for its morphology and size. The experimental method of research was used using three treatments and replicates of the different concentrations of Alagaw leaf extract: Treatment A (0.2 g/mL), Treatment B (0.4 g/mL) and Treatment C (0.6 g/mL) with 10 minutes and 60 minutes interval of observation under UV-vis spectrophotometer. Based on the findings of the study, Alagaw plant can potentially act as a good reducing agent for the biosynthesis of silver nanoparticles. The results recorded from UV-vis spectrophotometer support the biosynthesis and characterization of silver nanoparticles that as the concentration of the leaf extract increases it significantly affect the wavelength peaks and absorbance peaks of the synthesized silver nanoparticles. Using the high-resolution Transmission Electron Microscopy, the size of silver nanoparticles measured 50 nm – 100 nm having near-spherical in shape. Keywords: Nanoparticles, Biosynthesis, Spectroscopy, Absorbance Spectrum
Scientific reports, 2017
The rewards of using plants and plant metabolites over other biological methods for nanoparticle synthesis have fascinated researchers to investigate mechanisms of metal ions uptake and bio-reduction by plants. Here, green chemistry were employed for the synthesis of silver nanoparticles (AgNPs) using leaf extracts of Ocimum Sanctum (Tulsi) and its derivative quercetin (flavonoid present in Tulsi) separately as precursors to investigate the role of biomolecules present in Tulsi in the formation of AgNPs from cationic silver under different physicochemical conditions such as pH, temperature, reaction time and reactants concentration. The size, shape, morphology, and stability of resultant AgNPs were investigated by optical spectroscopy (absorption, photoluminescence (PL), PL-lifetime and Fourier transform infrared), X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). The enhanced antibacterial activity of AgNPs against E-Coli gram-negative bacterial strains ...