Emended description of Streptococcus ferus isolated from pigs and rats (original) (raw)
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Streptococcus porci sp. nov., isolated from swine sources
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2010
Two unidentified Gram-positive, catalase-negative, coccus-shaped organisms were recovered from pigs and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Streptococcus, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequence studies confirmed this identification and showed that the nearest phylogenetic relatives of the unknown cocci were Streptococcus plurextorum 1956-02T and Streptococcus suis NCTC 10234T (97.9 and 96.0 % 16S rRNA gene sequence similarity, respectively). The new isolates were related most closely to S. suis CIP 103217T based on rpoB gene sequence analysis (<8 % sequence divergence). DNA–DNA pairing studies showed that one of the unidentified strains (2923-03T) displayed DNA relatedness values of 26.6 and 27.2 % with S. plurextorum CECT 7308T and S. suis NCTC 10234T, respectively. On the basis of phenotyp...
Streptococcus porcorum sp. nov., isolated from domestic and wild pigs
International journal of systematic and evolutionary microbiology, 2011
Seven isolates of an unidentified Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from domestic and wild pigs were characterized by phenotypic and molecular-genetic methods. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Streptococcus, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequencing showed that the unknown bacterium was phylogenetically closely related to, but distinct from, Streptococcus suis (97.5 % 16S rRNA gene sequence similarity to the type strain). rpoB and sodA sequence analysis showed minimum interspecies divergence from phylogenetically close 16S rRNA gene sequence-based relatives of 13.8 and 18.6 %, respectively. DNA-DNA hybridization of a strain of the unidentified organism demonstrated 8-18 % reassociation with S. suis NCTC 10234(T). The novel bacterium could be distinguished from S. suis and other Streptococcus species ...
Streptococcus cuniculi sp. nov., isolated from the respiratory tract of wild rabbits
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2014
Biochemical and molecular genetic studies were performed on four unknown Gram-stain-positive, catalase-negative, coccus-shaped organisms isolated from tonsils (n53) and nasal samples (n51) of four wild rabbits. The micro-organism was identified as a streptococcal species based on its cellular morphological and biochemical tests. Comparative 16S rRNA gene sequencing confirmed its identification as a member of the genus Streptococcus, but the organism did not correspond to any recognized species of this genus. The closest phylogenetic relative of the unknown cocci from wild rabbits was Streptococcus acidominimus NCIMB 702025 T (97.9 % 16S rRNA gene sequence similarity). rpoB and sodA sequence analysis of the novel isolate showed interspecies divergence of 16.2 % and 20.3 %, respectively, from the type strain of its closest 16S rRNA gene phylogenetic relative, S. acidominimus. The novel bacterial isolate could be distinguished from the type strain of S. acidominimus by several biochemical characteristics, such as the production of esterase C4, acid phosphatase and naphthol-AS-BI-phosphohydrolase and acidification of different sugars. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as a novel species of the genus Streptococcus, Streptococcus cuniculi sp. nov. The type strain is NED12-00049-6B T (5CECT 8498 T 5CCUG 65085 T ).
Molecular typing of Streptococcus suis from pigs in Cuba
Biotecnología Aplicada, 2013
Streptococcus suis is a bacterium commonly carried by pigs in the respiratory tract; thus the infections caused by virulent strains are considered a problem in the swine industry. A successful approach for the identifi cation of virulent strains is the differentiation of capsular serotypes using specifi c antisera or the corresponding cps types by geno- typic assessment, with the subsequent detection of virulence associated factors, namely the extracellular factor, the muramidase-released protein and the hemolysin suilysin. Data regarding serological and molecular identifi cation of S. suis from pigs in Cuba are not available. This study was aimed at identifying the capsular types cps2, 7 and 9, as well as three genes related to virulence using PCR assays. According to the results, 31 isolates were evaluated and classifi ed as cps2 (n = 21) or cps9 (n = 4), while six isolates not were typable. Considering the presence in these isolates of the genes mrp, epf and sly, six different ge...
Journal of Clinical Microbiology, 1996
The present study was designed to comparatively investigate 34 beta-hemolytic streptococci isolated from infected pigs and monkeys from various islands in Indonesia. According to the serological and biochemical data, all 34 isolates were Lancefield's serological group C streptococci and could be identified as Streptococcus equi subsp. zooepidemicus. Of the 34 group C streptococci investigated, 28 grew on solid media in large, mucoid colonies, in fluid media at a uniform turbidity, and in soft agar in diffuse colonies. A decapsulation test with a hyaluronidase-producing Staphylococcus aureus strain revealed the hyaluronic acid nature of the capsular material. The remaining six streptococci grew on solid media in small, nonmucoid colonies, in fluid media as sediment with clear supernatant, and in soft agar in compact colonies. Determination of surface hydrophobicity by salt aggregation revealed a hydrophilic surface for the encapsulated bacteria and a hydrophobic surface for the unencapsulated group C streptococci. To further analyze the epidemiological relationships, all 34 mucoid and nonmucoid isolates from pigs and monkeys were subjected to protein and DNA fingerprinting. The latter was performed by pulsed-field gel electrophoresis. The protein profiles of all 34 isolates and the DNA profiles of 32 isolates appeared to be identical, with the DNA profiles of 2 isolates being closely related, indicating that a single virulent clone is responsible for this disease outbreak in Indonesia. At the beginning of 1994, a disease outbreak among pigs and monkeys was reported on the island of Bali, Indonesia. The diseased animals showed such clinical symptoms as painful swelling of the joints, respiratory disturbances, and diarrhea. Most of the animals died within a few days. The postmortem examinations of the pigs and monkeys revealed signs of polyarthritis, bronchopneumonia, pleuritis, epicarditis, endocarditis, and meningitis (6). Further bacteriological examinations resulted in the isolation of streptococci of serological group C. These bacteria seemed to be one of the major causative agents. These group C streptococci could be isolated from most of the pigs and monkeys in pure cultures. The first cases were reported among animals of a pig owner in a small village on the island of Bali. In the following weeks and months, the outbreak spread rapidly to the surrounding districts in Bali and into a monkey population. Within 3 months, comparable cases were reported for diseased pigs on the islands of Sumatra and Sulawesi. No comparable disease was reported in Indonesia before 1994. At present, little is known about the properties and epidemiological relationships of these group C streptococcal isolates from various places in Indonesia. The present study was designed to further characterize pig and monkey isolates from Bali and the islands of Sumatra and Sulawesi. MATERIALS AND METHODS Bacterial isolates. A total of 34 beta-hemolytic streptococci were investigated in this study. Thirty streptococci were isolated from diseased pigs from small farms in Bali, Sumatra, and Sulawesi, Indonesia, and four streptococci were isolated from diseased monkeys from two Bali monkey resorts over a time period of 4 months (April to July) in 1994. Before being sent to Germany, the bacteria were stored in liquid nitrogen. The bacteria were cultivated on Columbia sheep
Predicción molecular de serotipos de Streptococcus suis aislados de granjas porcinas en México
Revista Mexicana de Ciencias Pecuarias, 2021
Infections caused by Streptococcus suis (S. suis) pose a problem for the pig industry worldwide. Pigs often carry multiple serotypes of S. suis in the upper respiratory tract, where S. suis is frequently isolated from. The clinical diagnosis of the infection is presumptive and is generally based on clinical signs, the age of the animal and macroscopic lesions. In the laboratory, identification of S. suis is performed biochemically, and then, serotyping is performed with antisera to determine the serotype, but these tests can be inconclusive. To date, there are few studies that have documented the presence and diversity of S. suis serotypes in Mexico. In the present study, it was characterized S. suis strains from Mexican pig farms using molecular approaches; samples were first processed by PCR of the gdh gene to detect S. suis. Positive samples were then subjected to a two-step multiplex PCR (cps PCR) to detect and characterize each strain; the first step consisted of a grouping PCR...
Folia Microbiologica, 2021
The aim of the present study was to characterize bacteria of the genus Streptococcus isolated from the oral cavity of the guinea pig as well as to assess the significance of these microorganisms as potential veterinary and human pathogens. Sixty-two streptococcal isolates recovered from 27 clinically healthy guinea pigs were examined genotypically by sequencing the 16S rRNA and groEL genes. Among these isolates, only 13 could be assigned to a species described previously (mainly Streptococcus parasanguinis, S. mitis and S. suis), and the majority of the remaining ones differed considerably from the streptococcal species known to date (16S rRNA and groEL sequence similarities were
International Journal of Systematic and Evolutionary Microbiology, 2015
Four isolates of an unknown Gram-stain-positive, catalase-negative coccus-shaped organism, isolated from the pharynx of four wild rabbits, were characterized by phenotypic and molecular genetic methods. The micro-organisms were tentatively assigned to the genus Streptococcus based on cellular morphological and biochemical criteria, although the organisms did not appear to correspond to any species with a validly published name. Comparative 16S rRNA gene sequencing confirmed their identification as members of the genus Streptococcus, being most closely related phylogenetically to Streptococcus porcorum 682-03T (96.9 % 16S rRNA gene sequence similarity). Analysis of rpoB and sodA gene sequences showed divergence values between the novel species and S. porcorum 682-03T (the closest phylogenetic relative determined from 16S rRNA gene sequences) of 18.1 and 23.9 %, respectively. The novel bacterial isolate could be distinguished from the type strain of S. porcorum by several biochemica...
Molecular confirmation and detection of virulence genes of Streptococcus suis from Pigs in Assam
The study was designed to determine the prevalence as well as virulence genes of Streptococcus suis in pigs maintained in organized and unorganized farming systems in and around Guwahati, Assam. A total of 497 samples were collected from clinically healthy (n=267) and diseased (n=230) pigs of varying age and either sex. Samples were processed for isolation of S. suis and their identification based on biochemical characteristics and further confirmation through PCR amplification of the housekeeping gene gdh (glutamate dehydrogenase) encoding S. suis capsular biosynthesis. Important virulence genes of S. suis namely, epf, mrp, sly, and arc A were detected by using multiplex PCR. All together seven isolates were confirmed as S. suis. But none of the investigated virulence genes could be demonstrated in the present study.