Biosorption of lead by indigenous fungal strains (original) (raw)
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Bioremoval of Lead by Some Fungi Isolated from Soil
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Eco-friendly biological removal of heavy metal pollution from liquid factories wastes or soil is a recent approach which using the biosorbention. It has lots of advantages over chemical approach like its low cost, more efficient, more effective, multiple uses etc. The fungi are one of the most desirable absorbents because of its cell wall characteristics. The present work, therefore, aimed to use Asprgillus niger, Alternaria alternata and Trichoderma harzianum isolated from lead-contaminated soil to remove lead from solution. The aim of this work was to study the resistance and removal of lead metal by the fungi. Soil sample collected from contaminated area by lead in Hilla city. Its tolerance lead metal ions was studied by growing fungus in different metal concentration. Bio absorption of lead by live fungal biomass was determined by Atomic absorption spectrophotometer. The results showed that A.niger and A.alternata grew at 1,800 ppm of lead, while T.harzianum grew at 1,400 ppm. W...
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Bioremediation of lead by lead-resistant microorganisms, isolated from industrial sample
Advances in Bioscience and Biotechnology, 2012
Lead contamination in water is a widespread problem throughout the world and results from industrial use and processing of lead ore. Bio-availability of lead can be hazardous for children and causes mental retardation. The use of lead free petrol is one measure to check this pollution, but this heavy metal is also present in industrial effluents and need to be removed before these effluents are discharged to natural land or water and as well as to the environment. Using bioremediation, bacteria could render lead nonbioavailable would provide an alternative option for detoxifying this contaminant in the environment. The property of some species of bacteria and algae, to extract metals from their surroundings, has been utilized to purify industrial effluents. The first step in devising a bioremediation strategy is to identify candidate bacterial strains capable of modifying the contaminant. Biotechnological approaches are recommended for extraction of metal forms can be grown in ponds where effluents (rich in heavy metals) are discharged. The microbes will extract the heavy metals and sequester them inside their cell membranes. The goal of the present study was to examine the capacity of lead resistant bacteria and bioremediation of lead contaminated water.
Ecology, Environment and Conservation, 2022
Heavy metals impose a severe environmental and public health hazard because of their toxic effects and their ability to incorporate in terrestrial and aquatic food chains. Biosorption has many disadvantages over conventional technologies in significant metal removal performance from large volumes of effluents. Fungal biosorption can effectively be used for the removal of metals from contaminated water and soil. Heavy metal tolerant fungal species were isolated from the polluted sites and the most tolerant fungal strain FI-01 was selected. The FI-01 strain was identified as Penicillium chrysogenum on the basis of morphological and microscopic characterization. The live (active) and dead (inactive) biomass was prepared for biosorption assay. The operating parameters viz., pH, temperature and initial metal ion concentration was optimized to 5.0, 35°C and 100 mg/l for maximum biosorption. The dead biomass has shown 23.2 percent more biosorption capacity. The biosorption data of dead biomass shows good fit with the Freundlich adsorption isotherm.
A Biosorption study of Lead by Aspergillus Fumigatus
MARKHOR (The Journal of Zoology), 2020
Heavy metals liberated by various industrial and agricultural processes are the major pollutants in soil, agricultural, marine, and industrial and even treated waste waters. Lead is greatly used in many industrial applications such as a storage battery manufacturing, printing, fuels, photographic materials, pigments and explosive manufacturing Objective: To assess the ability of Aspergillus fumigatus for removal of metal contamination such as lead by biosorptionMethods: The pure culture of the Aspergillus fumigatus was used for biosorption. The initial samples were cultured on the bread as the fungal spores were given suitable environmental conditions i.e., temperature, moisture, pH etc. For obtaining the pure culture of the Aspergillus fumigatus culture media was prepared. The spores collected were then allowed to grow on a specific culture media in a sterile Petri plates under aseptic conditions. Inoculation will be carried out by using 20 loops of fungal spores spread on the cult...
Biosorption and Bioaccumulation of Copper and Lead by Heavy Metal-Resistant Fungal Isolates
Arabian Journal for Science and Engineering, 2015
Microorganisms play an important role in the bioremediation of heavy metal-contaminated wastewater and soil. In this research, isolation of heavy metal-resistant fungi was carried out from wastewater-treated soil samples of Hudiara drain, Lahore. The purpose of the present investigation was to observe fungal absorption behavior toward heavy metal. The optimum pH and temperature conditions for heavy metal removal were determined for highly tolerant isolates of Aspergillus spp. along with the initial metal concentration and contact time. Biosorption capacity of A. flavus and A. niger was checked against Cu(II) and Pb(II), respectively. The optimal pH was 8-9 for A. flavus and 4-5.4 for A. niger, whereas optimal temperature was 26 and 37 • C, respectively. Moreover, the biosorption capacity of A. flavus was 20.75-93.65 mg g −1 for Cu(II) with initial concentration 200-1400 ppm. On the other hand, biosorption capacity of A. niger for Pb(II) ranged from 3.25 to 172.25 mg g −1 with the same range of initial metal concentration. It was also found that equilibrium was maintained after maximum adsorption. The adsorption data were then fitted to Langmuir model with a coefficient of determination >0.90. The knowledge of the present study will be helpful for further research on the bioremediation of polluted soil.
Biosorption of Copper and Lead by Heavy Metal Resistant Fungal Isolates
Microorganisms play a significant role in bioremediation of heavy metal contaminated soil and wastewater. In this study heavy metal resistant fungi were isolated from the waste water treated soil samples of Hudiara drain, Lahore. The optimum pH and temperature conditions for heavy metal removal were determined for highly tolerant isolates of Aspergillus species along with the initial metal concentration and contact time. Biosorption capacity of Aspergillus flavus and Aspergillus niger was checked against Cu (II) and Pb (II) respectively. The optimal pH was 8-9 for A. flavus and 4-5.4 for A. niger, whereas the optimal temperature was 26°C and 37°C respectively. Moreover, the biosorption capacity of A. flavus was 20.75-93.65 mg/g for Cu (II) with initial concentration 200-1400 ppm. On the other hand the biosorption capacity of A. niger for Pb (II) ranged from 3.25-172.25 mg/g with the same range of initial metal concentration. It was also found that equilibrium was maintained after maximum adsorption.
Biosorption of lead from aqueous solution by fungal biomass of Aspergillus niger and Rhizopus sp
2016
The biosorption of lead from aqueous solution by pretreated fungal biomass of Aspergillus niger and Rhizopus sp. with NaOH was investigated. Parameters affecting biosorption such as pH, temperature and contact time were examined. It was found that the initial pH of the solution strongly affected to the degree of biosorption. The pH of 5 and 6 were the optimum pH on biosorption of Rhizopus sp. and A. niger, respectively. The wide range of temperature of 30-60°C showed slightly effect on lead adsorption. The rapid rate of adsorption was found in during the first 60 min and was remained nearly constant afterwards. The optimum contact time was between 60 and 90 min. Moreover, the lead adsorption by Rhizopus sp. was higher than that of A. niger in all cases of the study conditions.
Lead Biosorption by a Bacterium Isolated from Industrial Effluents
International Journal of Microbiology Research, 2012
An attempt was made to isolate microorganisms from the industrial effluents and to study their role in bioremediation of lead in selected industrial effluents. Sixty bacterial strains from the industrial effluent were isolated, purified, preliminarily identified and examined for their ability to uptake lead. Based on the minimum inhibition concentrations (MICs) of lead for the selected isolates, six strains were found to be most resistant isolates and amongst them one isolate showed maximum resistance to lead which was identified as Bacillus cereus. Scanning electron microscopic (SEM) photographs and Energy dispersive X-ray spectroscopy (EDS) signature of Bacillus cereus revealed that lead was adsorbed to the cell surface, confirming biosorption capacity of the bacteria.