Ultrastructure of the Cyst Wall of Sarcocystis Sp. In Roe Deer (original) (raw)
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1990
Sarcocystis tissue cysts from the Siberian roe deer (Capreolus pygargus) were studied by light and electron microscopy. Seven out of eight roe deer were infected (85 % ) . Cysts were macroscopically visible measuring 1-4 mm in length and 0.1-0.5 mm in width. They contained typical Sarcocystis merozoites 10.8 fim in length and 2.4 /xm in width. The taxonomic relation of Sarcocystis sibirica (Matchulski, 1947) to Sarcocystis species of the European roe deer (Capreolus capreolus) is discussed.
Assiut Veterinary Medical Journal, 2018
This study was conducted to determine the infection rate of Sarcocystis species in sheep in Assiut. A total of 100 slaughtered sheep examined for Sarcocystis, no macroscopic cysts were observed, 93% (93/100) were positive by microscopical examination and 28% (28/100) were positive by using Agar gel diffusion test. The infection rate of Sarcocystis had very high significant differences between microscopic examination and Agar gel diffusion test. High statistical significant effect was found on the infection rate of Sarcocystis in different examined muscles of sheep. The highest infection rate was recorded in oesophagus (71%), followed by diaphragm (65%), tongue (58%), skeletal muscles (53%) and heart muscles (43%). The infection rate of Sarcocystis in males was 95.2% (40/42) while in females were 91.4% (53/58) by microscopical examination. Higher infection rate of Sarcocystis were detected in sheep 6 months-2 years age 95% (38/40) than that of sheep between 2-4 years old 92.7% (38/41) and those equal or older than 4 years 89.5% (17/19). No significant difference between infection and age groups or sex of animals was observed. The use of transmission electron microscope (TEM) allowed the identification of S. tenella and S. arieticanis in sheep in Assiut. Examination of S. tenella cyst wall with TEM showed palisade-like villar protrusions while, examination of S. arieticanis cyst wall showed hair-like villar protrusions parallel to cyst wall.
2021
The macroscopic, histological, and molecular aspects of Sarcocystis spp. were examined in the tissues of two cattle and four sheep, 16 and eight fragments analyzed respectively, condemned in the slaughterhouse. All 24 samples were collected and analyzed for detecting macrocysts and macroscopic lesions. Subsequently, subdivided for direct examination, polymerase chain reaction and histopathological examination. All sheep tissues samples had grossly white round to oval tissue cysts, ranging from 0.3 to 1 cm in diameter. In contrast, cattle tissues did not present grossly visible cysts but had randomly distributed white-yellow foci with irregular contours. All samples from cattle and sheep had microscopic cysts. In the histological examination of sheep tissues, circular to elongated, encapsulated, basophilic structures ranging from 30 to 3,000 µm in length and 20 to 1,000 µm in width were observed within the skeletal muscle fibers. In cattle tissues, all cardiac muscle four fragments a...
International journal of molecular and cellular medicine, 2014
Sarcocystis is an obligatory intracellular protozoan parasite which can infect humans and animals. Sheep are intermediate hosts of four Sarcocystis species: Sarcocystis tenella, Sarcocystis gigantea, Sarcocystis arieticanis, and Sarcocystis medusiformis The purpose of this study was to perform a molecular identification of the macroscopic and microscopic cysts of Sarcocystis in sheep. In this investigation, the macroscopic and microscopic cysts of Sarcocystis were assessed in slaughtered sheep. The digestion method was used for bradyzoites observation in heart, liver, diaphragm and muscle samples. PCR analysis was conducted on macroscopic and microscopic cysts and also all other samples. Sequencing was performed for ten PCR products. Genotypes were identified by BLAST search and homology analysis. Macrocysts were seen in two muscle tissues. Digestion method and PCR analysis revealed positive results in all samples taken from heart, liver, diaphragm, and muscle. Genotyping of ten tis...
2014
Percoll gradient centrifugation can purify different parasitic stages from tissues or faeces of hosts. Sarcocystis tenella and Sarcocystis arieticanis develop microscopically visible cysts in sheep muscles. In this study, the distribution of microscopic cysts were determined in different muscles groups of sheep naturally infected with Sarcocystis spp. by using percoll gradient centrifugation. Sarcocystis spp. microscopic cysts were detected as 91% of sheep. S.tenella and mixed infections with S.tenella and S.arieticanis were observed in the tissue samples at a prevalence of 91% and 18.7%, respectively. Sarcocystis species cysts were most prevalent in the tongue muscle tissue at a rate of 80%. The cysts were observed a rate of 73%, 69% and 61% in the masseter, intercostal muscles and diaphragm, respectively. The relationship between the cysts present and the different muscles groups was significantly different (p<0.001). The number of microscopic cysts ranged from 4-476 (mean 235)...
Parasitology Research, 2009
In the present study, the heteroxeneous life cycle of Sarcocystis sp. infecting camels were studied. A total of 180 slaughtered camels collected from different localities in Egypt were investigated for sarcocysts. Only 116 animals were found to be infected (the infection rate was 64%). Muscle samples of esophagus, diaphragm, tongue, skeletal, and heart muscles were examined. Exclusively, microscopic sarcocysts were detected in all examined organs. The infection rates of the esophagus, diaphragm, tongue, skeletal, and heart muscles were 60%, 50%, 40%, 40%, and 10%, respectively. By means of transmission electron microscopy, details of the ultrastructure of the sarcocysts were studied. The specific architecture and ornaments of the cyst wall, its protrusions, and the cyst interior were recorded. Unique features of protrusions of the primary cyst wall, the knob-like structures, arise around each protrusion. Experimental infection of carnivores by feeding heavily infected camel muscles revealed that the dog, Canis familiaris, is the only final host of the present Sarcocystis species. Gamogony, sporogonic stages, and characteristics of sporulated oocysts were also investigated.
Animal and Veterinary Sciences, 2020
Background: Protozoal diseases caused by Sarcocystis species can cause serious damage in sheep and goats flocks, inducing decreased growth conversion rates and partial or complete condemnation of carcasses at the slaughter house. Sarcocystis spp. affecting sheep and goats are host specific for their intermediate hosts and family specific for their final hosts. They derive their name from the intramuscular cyst stage present in the intermediate host. Objective: Macro and micro identification of macro-cysts of Sarcocystis spp. affecting a Najdi sheep carcass. Method: Detection of macrosarcocystis during routine post mortem examination and identification the detected cyst by histopathological investigation. Result: The macroscopic sarcocystis were found cleared and scattered in almost all skeletal muscles including shoulder, chest and diaphragm as well as thigh. Both morphological and histopathological characteristics indicated that the detected cysts may be identified as Sarcocystis gigantea. Conclusion: It is a case study of macro-cysts of Sarcocystis spp. affecting local breed of Najdi sheep. It is considered a seldom detected case all over many years during meat inspection at Buraidah slaughter house. The possible control measures are those of simple hygiene. Farm dogs should not be housed in, or allowed access to, fodder stores nor should they be allowed to defecate in pens where livestock are housed. It is also important that they are not fed uncooked meat.