Studies on Precursors of Pyrimidines of Nucleic Acid (original) (raw)

Chemical Biology of Nucleic Acids

2014

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Part VII Purification of Nucleic Acids from Miscellaneous Sources

Handbook of Nucleic Acid Purification, 2009

Although many procedures have been developed for the purification of nucleic acid fragments, the most widely applied technique involves their separation by electrophoresis on an agarose or polyacrylamide gel followed by recovery using a variety of methods. The nucleic acid fragments isolated with these procedures have different levels of purity and can be used in many subsequent molecular biological manipulations. This chapter examines the fundamental aspects concerning the separation of nucleic acids using agarose and polyacrylamide gel electrophoresis (PAGE), and reviews techniques for subsequent recovery of DNA and RNA from agarose and polyacrylamide gels. It then goes on to present several protocols that have proven reliable and highly efficient in our own and other authors’ experience, and finally discusses areas where future refinements will lead to more speedy and effi cient recovery of nucleic acid fragments from gels.

A New Nucleotide and a New Polynucleotide from Bacteriophage Nucleic Acid

Journal of Biological Chemistry, 1954

Deoxyribonucleic acid (DNA) derived from most biological materials contains principally the four bases thymine, cytosine, adenine, and guanine. Studies by Wyatt and Cohen (1) on the deoxyribonucleic acid of the bacterial virus, T2r+, have revealed the absence of one of these, cytosine, and the presence of a new base, hydroxymethylcytosine, in its place. This communication deals with the isolation and identification of a nucleotide and a polynucleotide, also from bacterial virus, which on hydrolysis yield this new pyrimidine base.

Electrophore-labeling and alkylation of standards of nucleic acid pyrimidine bases for analysis by gas chromatography with electron-capture detection

Journal of Chromatography A, 1984

The pyrimidine bases cytosine, uracil and thymine, along with some analogues, are electrophore-labeled either with pentafluorobenzoyl chloride (PFBC), pentafluorophenylsulfonyl chloride (PPSC), or heptafluorobutyric anhydride. Subsequent alkylation is most successful for PFB-cytosine, PPS-uracil, and PPS-thymine. These same alkylated compounds also have the highest aqueous stability and respond most strongly by gas chromatography-electron-capture detection. One of these derivatives, determined to be N4-PFB-1,3-dimethylcytosine by authentic synthesis, and its 5methyl analogue, can be detected with good precision down to the lOO-fg level. Poor reproducibility is encountered at the IO-fg level.

Nucleic Acids Research

Nucleic Acids Research, 2008

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Interaction of Nucleic Acids, I. Physical Binding of Thymine, Adenine, Steroids, and Aromatic Hydrocarbons to Nucleic Acids

Proceedings of the National Academy of Sciences, 1964

In a previous communication,' it was shown that the helix-coil transition temperature, Tm, of thymus DNA and helical poly A is lowered by pyrimidines, purines, nucleosides, their analogues, and derivatives. The order of their effectiveness indicates that hydrophobic and stacking interactions are important.

Chemical and Biochemical Properties of Oligonucleotides that Contain (5′S,6S)-Cyclo-5,6-dihydro-2′-deoxyuridine and (5′S,6S)-Cyclo-5,6-dihydrothymidine, Two Main Radiation-Induced Degradation Products of Pyrimidine 2′-Deoxyribonucleosides

Tetrahedron, 2000

AbstractÐThe ®rst chemical synthesis of (5 H S,6S)-cyclo-5,6-dihydro-2 H -deoxyuridine [(5 H S,6S)-CyclodHdUrd], a major product of gamma irradiation of oxygen free aqueous solution of 2 H -deoxycytidine is reported. Subsequently, the latter cyclonucleoside was incorporated in de®ned sequence oligodeoxyribonucleotides. The chemical composition of the modi®ed DNA fragments was assessed by enzymatic digestions and mass spectrometry measurements. The latter analyses con®rmed the presence and the integrity of the lesion within the synthesised DNA fragments. Replication and repair studies showed that (5 H S,6S)-CyclodHdUrd together with (5 H S,6S)-CyclodHThd [(5 H S,6S)-cyclo-5,6-dihydrothymidine] inserted into DNA oligomers act as blocks for DNA polymerases and are not excised by DNA Nglycosylases. q