Screening of the needles of different yew species and cultivars for paclitaxel and related taxoids (original) (raw)
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Journal of Liquid Chromatography & Related Technologies, 2009
Four types of solvent extraction methods (ultrasound and microwave assisted extraction, pressurized liquid extraction, and extraction in the Soxhlet apparatus) for paclitaxel, cephalomannine and 10-deacetylbaccatin, taxoids recovered from common yew twigs, were compared. By use of pressurised liquid extraction the most effective extractant of taxoids was determined. HPLC was used for the analysis of extracts. Comparison of the obtained results revealed differences in the extraction power of the applied methods. The greatest yields were obtained by multiple PLE, which can be recommended as the best sample preparation method for taxoids analysis in yew twigs.
Analysis of taxol and major taxoids in Himalayan yew, Taxus wallichiana
Journal of Chromatography A, 1999
A reversed-phase column liquid chromatography method for the analysis of taxol, 10-deacetylbaccatin III, baccatin IV, 1-hydroxybaccatin I, 2-acetoxybrevifoliol, brevifoliol, 2′-deacetoxydecinnamoyltaxinine J and 2′-deacetoxytaxinine J in yew needles has been developed using a Nova-Pak Phenyl column and a binary gradient profile. The various aspects of analysis such as extraction efficiency, detection limits, reproducibility and peak purity were validated using UV–Vis as well as photodiode array detection.
Phytochemical Analysis, 1997
An analytical procedure for the large-scale qualitative and quantitative screening of paclitaxel (Taxol ® ) and related neutral taxanes in yew needles has been developed. The needles were selectively extracted with ethanol:water:acetic acid (80:19:1) and the resulting extract was purified from chlorophyll, 3,5-dimethoxy phenol, taxine alkaloids and cinnamoyl taxanes, by means of small-scale partition chromatography on Extrelut ® . After concentration, the taxanes were determined by high pressure liquid chromatography on a C 18 column with acetonitrile:water as eluent and ultra violet detection at 227 and 280 nm. Cinnamic acid was used as an internal standard. Various aspects such as extraction efficiency, recovery, peak purity, detection limits, robustness and reproducibility of the method have been evaluated. The methods is very labour efficient with only 20 min of manpower input per sample.
Phytochemical Analysis, 1999
Clippings of Taxus canadensis were collected and divided into needles, twigs and whole clippings samples. The biomass was extracted and analysed for taxanes using high performance liquid chromatography. The addition of n-octylbenzamide as an internal standard was found to reduce the standard deviation of calculated yields by a third. Paclitaxel was found to be highest in needles (0.0434% dry wt). The presence of paclitaxel was confirmed by photodiode array spectra. Impurities were found to co-elute with other taxanes. A compound was tentatively identified as 9-dihydro-13-acetylbaccatin III based on residence time and UV spectra. The effect of storage on paclitaxel content was investigated and it was found that paclitaxel was stable in dried needles stored at room temperature for at least 1 year.
Sex influences the taxanes content in Taxus baccata
Acta Physiologiae Plantarum, 2012
Like other species of the genus Taxus, European yew trees contain taxanes, including paclitaxel (T) and its precursor 10-deacetylbaccatin III (10-DAB). Taxanes are one of the most effective anticancer drugs. This study was undertaken to describe the levels and patterns of taxane variation in the Sudetian region (SW Poland). Paclitaxel (T) and 10-deacetylbaccatin III (10-DAB) concentrations were analysed in five populations. Needles and twigs were analysed from 60 individuals (30 males and 30 females) in each population. In addition, morphometric measurements were taken in the populations to obtain light intensity coefficients (specific leaf area, SLA). High variability in the taxane contents at both intra and interpopulational levels was found. Nevertheless, females had a significantly higher taxane content compared to males. Because taxanes are carbon-based secondary metabolites, females have higher rate of gas exchange of females compared to males. This was probably an adaptation to greater reproductive effort incurred by females. In this regard, female individuals seem to be better for selecting elite cultivars with a higher taxane production. The relationship between light intensity and taxane content was not significant. Shading, important for optimizing crop production, should not reduce the concentration of taxanes. Keywords Taxus baccata Á Taxanes Á Sex Á Dioecy Á Light intensity Abbreviations 10-DAB 10-deacetylbaccatin III T Paclitaxel SLA Specific leaf area SPE Solid phase extraction Communicated by J. V. Jorrin-Novo.
Journal of Chromatography A, 2001
An HPLC method was developed for quick scanning of taxanes from large numbers of plant cell suspension samples. The method was optimized for analysis of a range of taxanes of differing polarity. Identification of a standard mixture of paclitaxel and 12 related taxanes was achieved in less than 15 min using a gradient mode and a Microsorb-MV C column. 8 The method was used to investigate the influence of several factors on stability and recovery of paclitaxel from suspension media and cultures of Taxus cuspidata cv Densiformis. Incubation time had the most significant influence on stability of paclitaxel, contributing 88% to the total variation. Shaking contributed 6% to the total variation. Light contributed only 0.25% to the total variation. Analysis of test samples of suspension cultures of T. cuspidata cv Densiformis over a 4 week period show paclitaxel, 10-deacetylbaccatin III,
10-Deacetylbaccatin III analogues from Taxus baccata
Journal of Natural …, 1995
A s m m . -T h e needles of the European yew, Taxus barrata, yielded two new analogues of 10-deacetylbaccatin 111, identified as 13-epi-10-deacetylbaccatin 111 141 and 2-debentoyl-2tigloyl-10-deacetylbaccatin 111 {5]. The crystal structure of baccatin 111 161, corresponding to the terpenoid core of paclitaxel, was determined.