Advances of Salivary Proteomics in Oral Squamous Cell Carcinoma (OSCC) Detection: An Update (original) (raw)
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PROTEOMICS, 2015
Head and neck cancers, including oral squamous cell carcinoma (OSCC), are the sixth most common malignancy in the world and are characterized by poor prognosis and a low survival rate. Saliva is oral fluid with intimate contact with OSCC. Besides non-invasive, simple, and rapid to collect, saliva is a potential source of biomarkers. In this study, we build an SRM assay that targets fourteen OSCC candidate biomarker proteins, which were evaluated in a set of clinicallyderived saliva samples. Using Skyline software package, we demonstrated a statistically significant higher abundance of the C1R, LCN2, SLPI, FAM49B, TAGLN2, CFB, C3, C4B, LRG1, SERPINA1 candidate biomarkers in the saliva of OSCC patients. Furthermore, our study also demonstrated that CFB, C3, C4B, SERPINA1 and LRG1 are associated with the risk of developing OSCC. Overall, this study successfully used targeted proteomics to measure in saliva a panel of biomarker candidates for OSCC.
Saliva as a Non-Invasive Diagnostic Medium in Proteomics for Oral Squamous Cell Carcinoma Detection
International Journal of Medical Reviews
Non-invasive diagnostic techniques are essential for the screening of oral cancer to reduce high mortality associated with it. The acknowledgement of various research advancements namely omic research and technological advances in the field of genomes has led to an increased use of saliva collection. Obtaining saliva for diagnosis is simple and non-invasive and is safe for both the health worker and the patient. It also permits easy and cost-effective storage methods. Around 3000 differently expressed proteins and peptides have been identified in various proteomic studies involving saliva, and hence, salivary proteomics is being progressively used for the early diagnosis of numerous diseases such as oral cancer, breast cancer, and several autoimmune disorders, among others. The salivary analysis riding on the recent advancements in technology, can prove to be of much more importance in near future.
The role of salivary biomarker as a diagnostic tool in oral cancer: a literature review
Intisari Sains Medis
Background: Saliva is an informative biological fluid that has gained a lot of interest because of its physiologic diagnostic medium. The recent findings suggest that saliva could be used as a biomarker in diagnosing oral cancer. This study aims to elaborate further on the role of salivary biomarker as a diagnostic tool in oral cancer through literature review. Methods: A total of 44 relevant kinds of literature were studied regarding saliva and oral cancer. The data collection for eligible articles were conducted from 1997 to 2019. Different database and manual search methods were used to find the topic-related articles. Results: The study of saliva as a biological matrix has been identified as a new landmark initiative in search of a useful biomarker to diagnose oral cancer through proteomics and transcriptomics. Most oral cancers are oral squamous cell carcinoma. Using saliva for early oral cancer detection in the search for new clinical markers is a promising approach because of its noninvasive sampling and easy collection method. Identification of this salivary biomarker could help to screen patients at risk, predict disease outcome and effectively contribute to planning treatment strategies. Conclusion: proteomics and transcriptomics molecules on the saliva could be used as a biomarker in diagnosing oral cancer.
Salivary Biomarkers for Detection of Oral Squamous Cell Carcinoma: Current State and Recent Advances
Current Oral Health Reports, 2014
Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity. Detection of OSCC is currently based on a thorough clinical oral examination combined with a biopsy for histological analysis. Most cases of OSCC are not detected until the cancer has developed into advanced stages; thus, a reliable early-stage diagnostic marker is needed. This literature review presents an overview of the status of current advances in salivary diagnostics for OSCC. Though many protein and mRNA salivary biomarkers have been identified that can detect OSCC with high sensitivity and specificity, the most discernable findings occur with the use of multiple markers. Studies that incorporate proteomic, transcriptomic, and potentially additional "omics", including methylomics, need to be initiated to bring technology to clinical applications and allow the best use of saliva in diagnosing OSCC.
A Review on Salivary Genomics and Proteomics Biomarkers in Oral Cancer
Indian Journal of Clinical Biochemistry
Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world. Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce the morbidity and mortality associated with this disease. Sensitive and specific biomarkers for oral cancer are likely to be most effective for screening, diagnosis, staging and follow-up for this dreaded malignancy. Unlike other deep cancers, oral cancer is located in oral cavity. Hence, the direct contact between saliva and oral cancer lesion makes the measurement of tumor markers in saliva an attractive alternative to serum and tissue testing. The DNA, RNA and protein molecules derived from the living cancer cells can be conveniently obtained from saliva. Thus, salivary biomarkers, a non-invasive alternative to serum and tissue-based biomarkers may be an effective modality for early diagnosis, prognostication and monitoring post therapy status. In the current post-genomic era, various technologies provide opportunities for high-throughput approaches to genomics and proteomics; which have been used to evaluate altered expressions of gene and protein targets in saliva of oral cancer patients. The emerging field of salivary biomarkers has great potentials to prove its clinical significance to combat oral cancer. Hence, we have reviewed importance of several salivary genomics and proteomics biomarkers for oral cancer.
Role of Salivary Biomarkers in Oral Cancer Detection
Advances in clinical chemistry, 2018
Oral cancers are the sixth most frequent cancer with a high mortality rate. Oral squamous cell carcinoma accounts for more than 90% of all oral cancers. Standard methods used to detect oral cancers remain comprehensive clinical examination, expensive biochemical investigations, and invasive biopsy. The identification of biomarkers from biological fluids (blood, urine, saliva) has the potential of early diagnosis. The use of saliva for early cancer detection in the search for new clinical markers is a promising approach because of its noninvasive sampling and easy collection methods. Human whole-mouth saliva contains proteins, peptides, electrolytes, organic, and inorganic salts secreted by salivary glands and complimentary contributions from gingival crevicular fluids and mucosal transudates. This diagnostic modality in the field of molecular biology has led to the discovery and potential of salivary biomarkers for the detection of oral cancers. Biomarkers are the molecular signatur...
THE VALUE OF SALIVARY BIOMARKERS IN ORAL CANCER DIAGNOSIS
Human saliva is not just the fluid of the oral cavity, it is considered to be the mirror of oral and systemic health. Oral pathology is a major public health problem, with a worldwide increase in incidence and morbidity. Diagnosis of the disease is a real challenge because of the lack of sensitive biomarkers and characteristics in serum or tissues. Salivary proteomic analysis is a direction for diagnosis and also for the treatment of various diseases and could be considered a new approach to cancer prevention strategy or other oral pathological conditions. Protein and nucleic acid molecules derived from oral tissues can be extracted from saliva. For diagnostic purposes, saliva offers serum benefits through non-invasive sampling as a valuable source of clinically relevant information because it contains specific biomarkers to the unique physiological aspects of oral disease as well as systemic pathology such as cancer, heart or infectious diseases.
Salivary Proteomics : A Promising Technology in Oral Oncology : A Brief Review
2015
Early stage detection of cancer is the key to provide a better outcome for therapeutic intervention. Most routine screening and diagnosis tools for oral cancer lack sufficient sensitivity and specificity and sometimes they are invasive. Proteomic technologies hold recently great promise in the search of new clinical biomarkers for the early detection and diagnosis of cancer. Proteomic study of human body fluid (saliva) holds promise as a non-invasive method to identify biomarkers for human oral cancer. This review article provides a brief overview of the importance of salivary proteome in early detection of oral oncology.
Identification of potential salivary biomarker panels for oral squamous cell carcinoma
Scientific Reports, 2021
Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers worldwide with the maximum number of incidences and deaths reported from India. One of the major causes of poor survival rate associated with OSCC has been attributed to late presentation due to non-availability of a biomarker. Identification of early diagnostic biomarker will help in reducing the disease morbidity and mortality. We validated 12 salivary proteins using targeted proteomics, identified initially by relative quantification of salivary proteins on LC–MS, in OSCC patients and controls. Salivary AHSG (p = 0.0041**) and KRT6C (p = 0.002**) were upregulated in OSCC cases and AZGP1 (p ≤ 0.0001***), KLK1 (p = 0.006**) and BPIFB2 (p = 0.0061**) were downregulated. Regression modelling resulted in a significant risk prediction model (p < 0.0001***) consisting of AZGP1, AHSG and KRT6C for which ROC curve had AUC, sensitivity and specificity of 82.4%, 78% and 73.5% respectively for all OSCC cases and 87.9...
Role of Salivary Biomarkers in Oral Cancer Detection- A Systematic Review
International Journal For Multidisciplinary Research, 2023
Introduction: Saliva is an informative biological fluid that has gained a lot of interest because of its physiologic diagnostic medium. The salivary secretions are under control of sympathetic and parasympathetic stimulation. It contains enzymes, proteins, biochemicals. It has been suggested to be ultrafiltrate of blood. The recent findings suggest that saliva could be used as a biomarker in diagnosing oral cancer. The study of saliva as a biological matrix has been identified as a new landmark initiative in search of a useful biomarker to diagnose oral cancer through and transcriptomes. Most oral cancers are oral squamous cell carcinoma using saliva for early oral cancer detection in the search for new clinical markers is a promising approach because of its noninvasive sampling and easy collection method. Identification of this salivary biomarker could help to screen patients at risk, predict disease outcome and effectively contribute to planning treatment strategies. Biomarkers in saliva can be used as for diagnostic & response to treatment in oral squamous cell carcinoma.in this review, we explored their application in this increasingly common disease. A systematic review of the literature was performed based on the English titles listed in the PubMed, EBSCO, Cochrane, Science Direct, ISI web Science, and SciELO databases using the keywords. This article may help to identify the potential biomarkers for screening and the molecular pathology analysis in the high-risk patients with the OSCC.