Inoculation with Azospirillum brasilense enhances the quality of mesquite Prosopis juliflora seedlings (original) (raw)
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FLORESTA
Inoculation of rhizobacteria in seeds and plants has been documented as a stimulant and promoter of germination and growth of agricultural crops, but there is little information on native forest species. Therefore, the present study aims to evaluate the potential of Azospirillum brasilense inoculation in the germination of seeds and initial growth of seedlings of native forest species. The following experiments were carried out: (I) germination of seeds in germination chamber, and (II) initial growth of seedlings in nursery, both with four forest species (Cassia leptophylla, Lafoensia pacari, Mimosa flocculosa and Schinus terebinthifolius). For the four species, in both experiments, two treatments were used: inoculation of seeds with concentrated solution of A. brasilense and no inoculation of seeds (control). Physiological aspects of seed germination and seedling vigor were evaluated in the first experiment (germination speed, and length and dry weight of seedlings), and seedling g...
Agronomie, 2001
The effects of the inoculation of chickpeas (Cicer arietinum L.) and faba beans (Vicia faba L.) with Azospirillum brasilense strain Cd were studied under different growth conditions. In greenhouse experiments with both legumes, inoculation with A. brasilense significantly enhanced nodulation by native rhizobia and improved root and shoot development, when compared with non-inoculated controls. Moreover, the bacterial treatment was shown to significantly reduce the negative effects on plant growth caused by irrigation with saline water. In field experiments, inoculation of chickpeas with A. brasilense peat-based inoculants also resulted in a significant increase in nodulation, root and shoot growth, and crop yield as compared with non-inoculated controls. Azospirillum brasilense / Cicer arietinum / Vicia faba / Rhizobium sp. Résumé-Effets de l'inoculation avec Azospirillum brasilense sur le pois chiche (Cicer arietinum L.) et les fèves (Vicia faba L.) dans différentes conditions de croissance. Les effets de l'inoculation des pois chiches et des fèves avec Azospirillum brasilense souche Cd ont été étudiés dans différentes conditions de croissance. Dans les expériences en serre avec les deux légumineuses, l'inoculation avec A. brasilense a augmenté de façon significative la nodulation par les rhizobia indigènes et amélioré le développement des racines et des tiges comparativement à des témoins non inoculés. De plus, il a été montré que le traitement bactérien réduit de façon significative les effets négatifs sur la croissance des plantes dus à l'irrigation avec de l'eau salée. Dans les expériences au champ, l'inoculation des pois chiches avec A. brasilense incorporé à de la tourbe a permis également un accroissement significatif de la nodulation, de la croissance des racines et des tiges et du rendement de la culture comparativement à un témoin non inoculé. Azospirillum brasilense / Cicer arietinum / Vicia faba / Rhizobium sp.
Growth promoting effect of Azospirillum brasilense on Casuarina cunninghamiana Miq. seedlings
Plant and Soil, 1991
The growth of Casuarina cunninghamiana seedlings was stimulated when inoculated with Azospirillum brasilense. This resulted in a higher biomass production than in uninoculated controls in the presence or absence of a non-nodulating strain of Frankia. Increase in whole plant dry weight was due to a significant increase in both shoot and root biomass, which corresponded with a higher total N content of the plants inoculated with Azospirillum. No such effects were observed under inoculation with a non-nodulating Frankia strain. These results suggest that the growth-promoting substances provided by A. brasilense may have enhanced the growth of Casuarina seedlings.
Tabebuia donnell-smithii Rose growth inoculated with mycorrhizal fungi and Azospirillum brasilense
Agrociencia, 2014
La explotación forestal sin regulación en bosques tropicales promueve la invasión de vegetación secundaria que compite con especies de mediano y lento crecimiento, limitando su regeneración natural. Muchos esfuerzos de reforestación fracasan por esta situación poniendo en riesgo el equilibrio del ecosistema por diferencias en densidad y distribución de especies. Con el fin de promover un mayor crecimiento en menor tiempo de la especie maderable primavera (Tabebuia donnellsmithii Rose) en condiciones de vivero, se evaluó el efecto de la inoculación con Rhizophagus intraradices (Schenck et Sm.) Walker et Schuessler, Glomus spp., y Azospirillum brasilense Tarrand, Krieg et Döbereiner. El vivero se estableció con semillas recolectadas en el Soconusco, Chiapas, México, en bolsas de plástico de 5 kg con una mezcla de suelo andosol-mólico y arena de río lavada (1:1) con 2 g de inoculante al momento del trasplante. Azospirillum brasilense tuvo una concentración de 910 6 bacterias g 1 y R. intraradices 40 esporas g 1 de suelo con 95 % de colonización al sistema radical. El diseño experimental fue completamente al azar y los tratamientos fueron los microorganismos individuales, combinados y un testigo, con cuatro repeticiones. Las variables morfológicas y fisiológicas del rendimiento, porcentaje de colonización radical micorrízica se registraron cada 28 d desde los 56 d hasta 168 d después de siembra. Las plantas con R. intraradices, la simbiosis doble A. brasilenseR. intraradices y Glomus sp. (V) presentaron mayor aumento de biomasa a 112 d (p0.05), mientras que el mayor contenido de nitrógeno se encontró en raíces de plantas inoculadas con R. intraradices y en el vástago con A. brasilense. El contenido de fósforo fue mayor con R. intraradices y A. brasilense en el vástago. Las diferencias significativas finales en altura de
1990
The capacity of Azospirillum brasilense to enhance the accumulation of K, P, Ca, Mg, S, Na, Mn, Fe, B, Cu, and Zn in inoculated wheat and soybean plants was evaluated by using two different analytical methods with five A. brasilense strains originating from four distinct geographical regions. A Pseudomonas isolate from the rhizosphere of Zea mays seedlings was included as a control. All A. brasilense strains significantly improved wheat and soybean growth by increasing root and shoot dry weight and root surface area. The degree of plant response to inoculation varied among the different strains of A. brasilense. All strains were capable of colonizing roots, but the best root colonizer, Pseudomonas sp., had no effect on plant growth. The numbers of organisms of Brazilian strains Sp-245 and Sp-246 colonizing roots were similar regardless of the host plant. Numbers of organisms for the other strains were directly dependent on the host plant. The main feature characterizing mineral accumulation in inoculated plants was that all inoculation treatments changed the mineral balance of the plants, but in an inconsistent manner. Enhancement of mineral uptake by plants also varied among strains to a great extent and was directly dependent on the strain-plant combination; i.e., a strain capable of increasing accumulation of a particular ion in one plant species or cultivar often lacked the ability to do so in another. Minerals in inoculated plants were not evenly distributed in different plant tissues, and the changes varied among groups of plants within each bacterial strain inoculation treatment. We suggest that, although A. brasilense strains are capable of changing the mineral balance and content of plants, it is unlikely that this ability is a general mechanism responsible for plant improvement by A. brasilense.
Acta Agronómica, 2016
One of the most promising techniques to promote seedling growth of tree species at nursery is the use of rhizosphere microorganisms. This is particularly relevant in enhancing plant performance in reforestation of degraded lands. A series of experiments were conducted to evaluate the effectiveness of either individual inoculation with the arbuscular mycorrhizal fungus Rhizoglomus fasciculatum (Thaxt.) Sieverding, Silva & Oehl or dual inoculation with R. fasciculatum and the phosphate solubilizing fungus Mortierella sp., at two doses (50 and 100 kg of inoculum per m 3 of substrate), on plant growth and phosphorus (P) uptake of Tecoma stans, Dodonaea viscosa, Fraxinus chinensis, and Lafoensia speciosa. These plant species are commonly used in Colombia in reforestation programs. The results indicated that treatments were effective to increase plant performance of the four plant species; however, the effect was significantly higher when both fungi R. fasciculatum and Mortierella sp. were concomitantly inoculated than when only the mycorrhizal fungus was applied. Overall, the dual inoculation at the dose of 50 kg m-3 had higher or similar effects than at 100 kg m-3. The results suggest that the dual inoculation was more effective in order to prevent plant P deficiency and stimulate plant growth.
2002
Arbuscular mycorrhizal fungi (AMF) are able to increase root enzymatic activity of acid and alkaline phosphatases. However, the role of AMF on phosphatase activity has not been reported in papaya (Carica papaya L.), which is frequently established at places with soil phosphorus (P) deficiencies. The goals of this research were to determine the effect of Glomus claroideum (Gc), and plant growth promoting rhizobacterium Azospirillum brasilense strain VS7 [Ab]) on root phosphatase activity and seedling growth of Carica papaya L. cv. Red Maradol under low P conditions. There were four treatments-colonization with: 1) Gc, 2) Ab, 3) Gc+Ab, and 4) non-inoculated seedlings. Plants were established in a coarse sand:sandy loam substrate under P-limitation (11µg P ml-1), supplied with a modified Long Ashton Nutrient Solution. Seedling growth was severely reduced by low P. Gc+Ab inoculated plants had greater total dry matter and leaf area than non-colonized plants. Gc-inoculated plants had greater leaf area than non-colonized plants. Treatments did not differ in leaf area ratio, specific leaf area and, total chlorophyll content. There was a non-significant effect on stem relative growth rate with Gc and Gc+Ab plants. Mycorrhizal colonization enhanced the bacterial population 3.4-fold in the Gc+Ab treatment compared with the population quantified in Ab treatment. Soluble and extractable root acid phosphatase activity (RAPA) was higher in Gc inoculated plants. We discussed on the possible relation among both inoculated microorganisms and also with the P-limitation which plants were established.