Effect of Temperature and Fermentation Time of Crude Cellulase Production by Trichoderma Reesei on Straw Substrate (original) (raw)
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TJPRC, 2013
The cellulase production in solid state fermentation (SSF) by Trichoderma viride was investigated using wheat straw and papyrus straw as the substrate. Present study described the optimization of process parameters for the production of cellulases. The fermentation experiments were carried out in shake flasks using pretreated wheat straw and papyrus straw. Maximum production of cellulases from wheat straw (CMCase 1.62 U/ml/min) and from papyrus straw (PAPase 0.81 U/ml/min) was observed after a fermentation period of 70hrs at an incubation temperature of 30ºC. Initial pH of the culture medium was also optimized and a pH of 5.5 was found to support maximum growth and enzyme production. Different inorganic nitrogen sources were evaluated for the production of cellulases and ammonium sulphate was found to be the best. The enzyme production was further enhanced by carrying out fermentation experiments using 25 ml of culture medium in 250 ml flask inoculated with 4% inoculum.
Enhanced cellulase production from Trichoderma reesei QM 9414 on physically treated wheat straw
Applied Microbiology and Biotechnology, 1986
Trichoderma reesei QM 9414 was grown on wheat straw as the sole carbon source. The straw was pretreated by physical and chemical methods. The particle size of straw was less than 0.177 mm. Growth of T. reesei QM 9414 was maximal with alkali-pretreated straw whereas cellulase production was optimal when physically pretreated straw was used as substrate. Cellulase yields expressed as IU enzyme activity/g cellulose present in the cultures were considerably higher when alkali pretreatment of wheat straw was omitted. Cellulase yields of 666 IU/g cellulose for filter paper activity (FPA) are the highest described for cultures of T. reesei QM 9414 carried out in analogous conditions. Crystallinity index of the cellulose contained in wheat straw increased slightly after alkali pretreatment. This increase did not decrease cellulose accessibility to the fungus. Delignification of wheat straw was not necessary to achieve the best cellulase production.
Journal of environmental biology / Academy of Environmental Biology, India, 2012
Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation (SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml(-1) using wheat bran as substrate. The optimal conditions forcellulase production forwheat bran were found to be: initial moisture content - 70%, initial medium pH-5.0, temperature-30 degrees C, moistening agents (MSS) and particle size of substrate (500 microm).The optimal incubation time for production was six days. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.
Cellulase production studies have been carried out using the fungal strain Trichoderma reesei NCIM 992 by using three different lignocellulosic materials by solid state fermentation (SSF). The effect of basic fermentation parameters (pH, temperature, moisture content, particle size of substrate and moistening agent) on enzyme production was studied. Maximum cellulase production was 2.63 U ml -1 using wheat bran as substrate. The optimal conditions for cellulase production for wheat bran were found to be: initial moisture content-70%, initial medium pH-5.0, temperature-30 o C, moistening agents (MSS) and particle size of substrate (500 µm).The optimal incubation time for production was six days. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the strain under SSF.
The Evaluation of Substrates and Trichoderma sp. Isolates for Cellulase Production
Jurnal Kimia Terapan Indonesia
As higher interest was on the lignocellulose-based or second generation bioethanol production, the research was then more focused on the production of cellulase, especially on the domestic enzyme. Trichoderma sp. is considered as one of the most efficient producer of cellulase. This study was conducted to investigate the performance of Trichoderma sp. on a variety of substrates to produce cellulase. Three types of substrate variations and three types of Trichoderma sp. were used in this experiment. The substrate used were wheat bran, rice bran and oil palm empty fruit bunches (EFBs), whereas Trichoderma sp. isolates were encoded as T004, T051 and T063. Production of cellulase was made by solid fermentation for 7 days. The analysis of cellulase activity was done by National Renewable Energy Laboratory (NREL) method for filter paper assay. The results showed that the type of substrate affected the performance of Trichoderma sp. All types of fungus produced cellulase on wheat bran subs...
2016
Research on production and the use of cellulase and xylanase by commercial microbial strains is widely reported. However, research on production of cellulase and xylanase by local isolates of Trichoderma harzianum known as potential cellulase producers is still very limited. T. harzianum SNRS3 was used for cellulase and xylanase production from rice straw under solid state fermentation. Our study revealed that unlike Trichoderma sp. that is normally associated with low amounts of β-glucosidase, insufficient to perform an efficient hydrolysis, T. harzianum SNRS3 could be considered as a potential β-glucosidase producer, but not an efficient xylanase producer. As a result of storage of the crude cellulase at room temperature, β-glucosidase activity only decreased to above 80% of its original activity at the end of the 3rd week of storage. The crude cellulase produced by T. harzianum SNRS3 could be industrially applied as the enzyme is still highly active at 60°C and over a wide range ...
Food and Bioproducts Processing, 2010
In the present study, the effect of culture conditions on cellulase activities by Trichoderma reesei QM9414 grown on rice bran media in solid-state fermentation was evaluated. Also, the optimum conditions were determined using response surface methodology (RSM). A face center design was used to investigate the effect of independent variables i.e. particle size, aeration rate and harvesting time on filter paper activity (FPA), Avicelase and carboxy methyl cellulase (CMCase) activities. The highest FPA, Avicelase and CMCase activities were 11.648, 99.756 and 94.210 U/g dry weight of substrate (DWS), respectively. The results showed that aeration rate and particle size had significant effects on the activity of cellulase on FPA. Also, particle size and harvesting time had significant effects on the activity of both Avicelase and CMCase. The reduction of particle size and harvesting time to 1-1.5 mm and 4 days, respectively increased cellulase activities.
Bioresource Technology, 2007
Response surface methodology (RSM) was used to evaluate the effects of fermentation parameters for cellulase production by Trichoderma reesei QM9414 and T. reesei MCG77 in solid-state fermentation using rice bran as substrate. Initial pH, moisture content and temperature were optimized using filter paper activity (FPA) as response. Statistical analysis of the results for T. reesei QM9414 showed that only moisture content had significant effect on cellulase activity and had a linear effect on enzyme activity (maximum enzyme activities were obtained at 70% moisture content). The results for T. reesei MCG77 showed that temperature and moisture content were the most significant parameters for cellulase activity. The optimum cellulase production was in the temperature range of 25-30°C and moisture content between 55% and 70%. After the optimization, the FPA in T. reesei MCG77 was increased by 2.5 folds compared to that of T. reesei QM9414.
2010
The purpose of the present work was to study the production and process parameters optimization for the synthesis of cellulase from Trichoderma viride in solid state fermentation (SSF) using an agricultural wheat straw as substrates; as fungal conversion of lignocellulosic biomass for cellulase production is one among the major increasing demand for various biotechnological applications. An optimization of process parameters is a necessary step to get higher yield of product. Several kinetic parameters like pretreatment, extraction solvent, substrate concentration, initial moisture content, pH, incubation temperature and inoculum size were optimized for enhanced production of third most demanded industrially important cellulase. The maximum cellulase enzyme activity 398.10±2.43 μM/mL/min was achieved when proximally analyzed lignocellulosic substrate wheat straw inocubated at 2% HCl as pretreatment tool along with distilled water as extraction solvent, 3% substrate concentration 40%...
Enhanced Production of Cellulase by Trichoderma reesei Using Wheat Straw as a Carbon Source
The objective of this study was the screening of a carbon source and microorganism for the enhanced production of cellulose enzyme system. Comparatively, untreated wheat straw (UWS) proved to be the best carbon source and the presence of lignin offered no hindrance to cellulase production by the fungus. Among single cultures, comparatively, T. reesei showed higher specific activity of CMCase and FPase in UWS than T. viride and other strains. The co-culture of T. reesei and T. viride showed better specific CMCase and FPase activity with treated wheat straw (TWS) (3.18 and 2.10) than UWS (1.05 and 1.24). Galactose proved to be a better inducer than glucose or Tween-20 and the specific activity of CMCase (19.21±0.82) and FPase (06.27±0.94) was increased while the production time was decreased from 96 to 72 h. The highest specific cellulase activity was achieved by T. reesei at 30±1°C, pH 6 and 2% four-day-old inoculum with 0.2% (w/v) galactose as the inducer. We achieved the objectives of enhanced production of cellulase using inexpensive lignocellulosic biomass from wheat straw to cut down production costs.