Journal of Biologically Active Products from Nature Study on Chemical Diversity Among Plant Parts of Zingiber chrysanthum and Their Antioxidant Assay (original) (raw)
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Study on Chemical Diversity Among Plant Parts of Zingiber chrysanthum and Their Antioxidant Assay
Journal of Biologically Active Products from Nature, 2017
The objective of present study was to analyze the chemical composition and antioxidant activity of hexane extract from different plant parts of Zingiber chrysanthum Rosc. GC/MS analysis revealed the vast chemical diversity with geranyl linalool as major constituent which varied quantitatively in different parts like rhizomes (27.8 %), seed (40.3 %) and perianth (3.8 %) along with other minor mono and sesquiterpenoids. The antioxidant activity of the hexane extracts was measured by their ability to scavenge free radicals, metal chelating and reducing power. The antioxidant of the extracts was found to have positive correlation with the total phenolic content of the extracts while IC 50 values exhibited negative correlation. The total phenolic contents were determined by Folin-Ciocalteu method using gallic acid as the standard. Among the extracts under study exhibited dose dependent antioxidant potential however DPPH radical scavenging activity was found maximum in with IC 50 value 187.93±0.75 μg/ml and negative correlation (-0.788) with phenols. Based on these data, it can be concluded that this medicinally important traditionally herb may be a good source of industrially important phytochemicals like geranyl linalool and natural antioxidant.
Antioxidant Activity Study of Zingiber Zerumbet Rhizome Extract and Fraction
ad-Dawaa' Journal of Pharmaceutical Sciences, 2022
The Zingiber zerumbet was identified as a member of the Zingiberaceae family with pharmacological potential. This study aims to characterise the phytochemical compound and evaluate the antioxidant potential of the methanol extract, ethyl acetate, and n-hexane-fractions of Zingiber zerumbet rhizome. Maceration with methanol was used for extraction, and the extract was then fractionated with n-hexane, ethyl acetate, and water. The phytochemical identification was conducted for alkaloids, saponins, flavonoids, polyphenols, and steroids/triterpenoids. Antioxidant activity was determined based on DPPH absorbance measurements using UV-Vis spectrophotometry. The methanolic extract contained alkaloids, flavonoids, polyphenols, and steroids/triterpenoids. The ethyl acetate fraction contained flavonoids and polyphenols, and the n-hexane fraction contained alkaloids and flavonoids. The free radical scavenging activities of ascorbic acid, methanol extract, ethyl acetate, and the n-hexane soluble fraction were 7.79; 100,956; 69,476, and 77,780 μg/mL, respectively. The findings indicated that the methanolic extract, ethyl acetate, and n-hexane soluble fraction contained antioxidant compounds.
Journal of Academia and Industrial Research (JAIR), 2015
Zingiber cernuum Dalzell is a perennial herb belonging to family Zingiberaceae, an important medicinal plant widely used in several indigenous medicinal formulations. In the present study, preliminary phytochemical and antioxidant activity of the rhizome extracts of Zingiber cernuum were evaluated. Phytochemical screening indicated that rhizomes are rich in a variety of primary and secondary metabolites such as carbohydrates, alkaloids, vitamin C, E, flavonoids, phenols, glycosides, saponins and minerals. Present investigation shows DPPH anti-scavenging activity in organic extracts such as methanol (56.33%), chloroform (21.14%), acetone (24.65%) and water (35.77%). The study highlights the biochemical and ethnopharmacological significance of Zingiber cernuum. Keywords: Zingiber cernuum, phytochemicals, rhizome extracts, secondary metabolites, anti-scavenging activity.
Evaluation of cytotoxicity and antioxidant properties of some Zingiberaceae plants
International Journal of Green Pharmacy, 2019
Aim: Zingiberaceae family is widely distributed in the tropical realm of Asia. Considering its diverse applications as spices and therapeutics, the present study was undertaken to evaluate the cytotoxic and antioxidant effect of the ethanolic rhizome extracts of five plants, namely Alpinia galanga (L.) Willd., Alpinia zerumbet (Pers.) B.L. Burtt and R. M. Smith, Curcuma caesia Roxb., Zingiber officinale Rosc., and Zingiber zerumbet (L.) Smith on Allium cepa Linn. system. Materials and Methods: Cytotoxicity was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) and 2’,7’-dichlorofluorescein diacetate (DCFDAH2) assays. Further, in vitro DNA protection assay was performed to confirm the antioxidant potentials of the extracts. Characterization of phytochemicals was done by performing qualitative tests. Results and Discussion: TTC reduction assay revealed that the extracts (2.5, 5, and 10 μg/ml) had no cytotoxic effect on A. cepa root cells. Roots treated with extracts (2.5 μg/ml) were stained with reactive oxygen species-sensitive dye DCFDAH2 and visualized under the fluorescence microscope. The result confirmed that the extracts did not exert any prooxidant effect. Further, the extracts established their substantial antioxidant potential by inhibiting oxidative DNA damage in an in vitro system. In addition, qualitative analysis showed that the rhizomes are rich in phytochemicals. Conclusion: From the current observations, it can be concluded that the selected herbs can be utilized safely for human consumption.
Nusantara Bioscience, 2016
Zingiberaceae is a family of plant with antioxidant, anticancer, and antiaging activities. The use of the leaves part of this family has never been explored. Therefore, the aim of this research is to evaluate the antioxidant and antiaging activities of some Zingiberaceae's leaves. The species of Zingiberaceae used in this study are Alpinia galanga (L), Curcuma aeruginosa Roxb, Curcuma longa L, Curcuma zedoaria, Curcuma xanthorrhiza Roxb, Boesenbergia rotunda (L.), Elettaria cardamomum, Zingiber zerumbet, Zingiber officinale Roscoe, and Zingiber purpureum Roscoe. Leaves were extracted with 2 different solvents sequentially: ethyl acetate and methanol. Total phenolic and flavonoid contents of the extracts were determined spectrophotometrically. Antioxidant activities were tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzothiazolin-6-sulfonic acid), whilst antiaging activity was tested by using antiglycation method. The results showed that the flavonoid content of the extracts were in the range of 0.83-25.17 g QE/100 g extract with total phenolic content ranging from 9.80-91.71 GAE mg/100 mg extract. The methanol extract of Curcuma xanthorrhiza displayed the highest antioxidant capacity in the DDPH (IC 50 282.35 mg/mL) and TEAC (10.08%) assays, followed by the ethyl acetate extract of C. longa, ethyl acetic extract of C. xanthorrhiza and methanol extract of Z. officinale, while the most active antiglycation is the methanol extract of Z. officinale (IC 50 203.85 mg/L).
Phytochemical study of selected Zingiberaceae plant species in the valley district of Manipur
2018
In the present study methanolic rhizome extract of the three plant species of Zingiberaceae namely Amomum subulatum Roxb., Boesenbergia longiflora (Wall.) Kuntze and Curcuma angustifolia Roxb.were evaluated for determination of total phenol, flavonoid and tannin content. Amomum subulatum Roxb. was recorded to have highest phenol content with 5.65 mg/g among the other two species. Flavonoid and tannin content was found highest in Boesenbergia longiflora (Wall.) Kuntze (1.8 mg/g) and Amomum subulatum Roxb. (3.52 mg/g) respectively. The presence of the high amount of phenol and flavonoid in the plant can be a potential source of antioxidant.
Pleione
Zingiber montanum (J.Koenig) Link ex Dietr. (Zingiberaceae) is a medicinal plant used by the indigenous people of Arunachal Pradesh, India. The rhizome is properly washed and boiled or eaten raw during fever, cold and cough. It isalso reported to be used against Jaundice. The 2, 2-Diphenyl-1-picrylhydazyl (DPPH) antioxidant screening was done using DPPH free radical scavenging activity in methanol, petroleum, and chloroform extract. Folin-ciocalteu's phenol reagent is used to estimate total phenol content by using Gallic acid standard curve. The total phenolic content of Zingiber montanum in methanol, petroleum ether and chloroform extracts showed 0.40±0.004, 0.35+ 0.002 and 0.032 + 0.003 GAE/g respectively and antioxidant activity was found 29.1 ± 0.03, 40.9 ± 0.04 and 84.3±0.02 in petroleum ether, chloroform and methanol extracts respectively which was compareble to the standard. Phytochemical screening also revealed the presence of alkaloids, saponins, tannins, flavonoids, terpenoid and phenolic compounds in its rhizome.
Antioxidant Activities and Cytotoxicity of Zingiber zerumbet (L.) Smith Rhizome
Zingiber zerumbet (L.) Smith, popularly known as shampoo ginger, is one of the most commonly used ingredients in Indo-Malaysian traditional medicines. The antioxidant and cytotoxic activities of the ethanolic extract of Z. zerumbet rhizome (ZZ) was investigated. 2,2-di(4-tert-octylphenyl)-1picryl-hydrazyl (DPPH) and hydroxyl radical scavenging assays showed significant radical scavenging activities of ZZ. The extract was rich in polyphenol and flavonoids. Cytotoxicity was assessed in vitro by trypan blue exclusion test. Human peripheral blood lymphocyte cells were incubated in different concentrations of ZZ (0, 15, 30, 60, 120, 300 and 600 µg/ml) for 3 h at 37 °C. The rhizome extract was found to be cytotoxic at concentrations higher for human consumption. In addition, HPLC analysis revealed ZZ as a rich source of kaempferol. Based on the results of the present investigation the rhizome may be used safely as a therapeutic antioxidant.
Ginger (Zingiber officinale Roscoe) is a herb that is well known and widely used, especially in Asia and Africa, which contains several interesting bioactive constituents and possesses health promoting properties. In this study, Phytochemical contents and antioxidant potential of Nigerian and Indian ginger were assessed in an effort to compare and validate the medicinal potential of the plant. Aqueous, hydro-methanolic and hydro-ethanolic extracts of Indian and Nigerian varieties were prepared by extraction process. Both varieties were found to contain high amount of secondary metabolites with exception of phlobotannins which happened to be in low quantity and tannins which is entirely absent in all the extracts. DPPH scavenging activity, Hydrogen peroxide and reducing power were assayed. The comparison carried out between different extracts of the two varieties using t-test at P=0.05 revealed a significant difference in terms of antioxidant activity. Indian variety was generally found to have more antioxidant activity than the Indian one with the exception of aqueous extract of Nigerian variety. This is probably due to high level of phenolics and flavonoids in the Indian variety. This study validated the medicinal potential of Zingiber officinale and established some relationship between the two varieties in terms of their antioxidant properties.