BIOCHEMICAL RESPONSE OF NORMAL ALBINO RATS TO THE ADDITION OF AQUEOUS LEAVES EXTRACT OF HIBISCUS CANNABINUS AND MURRAYA KOENIGII IN RATS DRINKING WATER (original) (raw)

Effect of Hibiscus sabdariffa extract Orally Administered on Lipid Parameters of Albino Rats

Journal of Medical Science and Clinical Research, 2016

Lipid parameters were investigated in albino rats of different groups after administration of 0.0mg/ml, 25.0mg/ml, 50.0mg/ml, 100.0mg/ml and 200.0mg/ml of aqueous extract of Hibiscus sabdariffa. The results obtained show that total cholesterol, triglyceride, high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) levels of the control group were 112.

Biochemical Effects of Oral Administration of Aqueous Extract of Hibiscus sabdariffa on Wistar Albino Rats

IOSR Journal of Dental and Medical Sciences, 2013

Hibiscus sabdariffa had been reported to have a broad range of therapeutic effects. The effects of oral administration of Hibiscus sabdariffa aqueous extract on some biochemical parameters were studied in wistar albino rats. A total of twenty five (25) male albino rats were grouped randomly into groups. Group A (control), Group B (0.6g/100ml of water of HS extract) Group C (1.2g/100ml HS extract), Group D (1.8g/100ml HS extract) and Group E (1.8g/100ml + vitamin C) and treatment period was 28 days. The results indicate an increase activity of liver function enzymes; alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST). This increase was statistically significant (p<0.05) when compared with Group A (control) in a dose dependent manner.No significant difference was observed in total and direct bilirubin in all the groups. Similarly for renal indices, bicarbonate (Hco 3), urea and creatinine in all the groups, but a significant increase (p<0.05) in sodium (Na+) and potassium (K+) and chloride were noted in comparison to the control.It is of note that there was no significant difference between results of Group E and the control (Group A). However, this study suggests that long term administration of the extract may indeed be toxic to liver and kidney especially at high doses.

The consumption of Hibiscus sabdariffa dried calyx ethanolic extract reduced lipid profile in rats

Plant Foods for Human …, 2005

The scientific basis for the statement that plants and their active constituents play an important role in the prevention of chronic and degenerative diseases is continously advancing. The object of the present study was to evaluate the effect of Hibiscus sabdariffa L. dried calyx ethanolic extract on the serum lipid profile of Sprague-Dawley rats. The rats were fed during 4 weeks with either a basal diet, containing high cholesterol (1%), cholic acid (0.25%), lard oil (10%), or a supplemental diet with H. sabdariffa extract at 5%, 10%, and 15% levels (SD 5 , SD 10 , SD 15 ). Weight gain and faeces dry weight were both very significantly less ( p ≤ 0.01) in SD 10 and SD 15 groups as compared to the control group, as was food efficiency in SD 15 . In the case of SD 10 , food efficiency was only significantly lower ( p ≤ 0.05). Faeces dry weight in SD 5 however, was the only one found to be significantly higher ( p ≤ 0.05). Triacylglycerols and LDL levels were both significantly less ( p ≤ 0.05) in all groups (SD 5 , SD 10 , and SD 15 ) as compared to the control. For total lipids, SD 10 and SD 15 showed significantly lower levels ( p ≤ 0.05), whereas very significant differences ( p ≤ 0.01) were observed in the case of SD 5 . All groups had lower cholesterol levels compared to controls; however, only the SD 5 group was statistically significant ( p ≤ 0.05). No significant results were found in any group in the cases of either phospholipid or HDL levels. A hypothesis of hibiscus acid racemization, (+)-HCA to (−)-HCA, mediated by intestinal flora enzymes possibly explains the significant triacylglycerol decrease in all experimental groups. VLDL, the precursor of LDL, is composed predominantly of triacylglycerols, suggesting that the significant decrease in LDL is related to observed triacylglycerol synthesis inhibition. Five percent H. sabdariffa extract addition showed the best results in the reduction of serum lipids under study conditions.

Hypolipidemic Activity of Aqueous Flower Extract of Hibiscus sabbdariffaL.inHigh Cholesterol Diet Fed Rats

The hypolipidemicactivity of aqueous extract for flowers of Hibiscussabdariffa L. was evaluated inhypercholesterolemicrats.Thirty two male adult rats were divided into four groups, each one involved eight rats. First group fed normal diet (normal group). The second group fed with high cholesterol diet (HCD) and received oral administration of 2 ml sterile water (control group). the third and fourth groups fed with high cholesterol diet(HCD) and received oral administration of aqueous extract of flowers of H. sabdariffa, 200 and 400 mg/kg respectively in 2 mL of sterile water for 21 days. The animals were sacrificed; Biochemical analysis of blood serum were performed for lipid profile and liver enzymes.The extract induces a significant decrease in serum cholesterol, triglycerides and Bloodlevels of AST, ALT, ALP. The results concluded that Hibiscus sabdariffaextract aqueous (200, 400 mg/kg) have potent hypolipidemicactivity inhypercholesterolemic rats.

Physicochemical Constituents, Phytochemical and Morphological Effects of Oral Administration of Aqueous Extract of Hibiscus sabdariffa on Kidney and Liver of Wistar Albino Rats

The physicochemical constituents, phytochemical and morphological effects of aqueous extract of Hibiscus sabdariffa (HS) were studied in wistar albino rats. Structural analysis of phytochemicals has provided the basis for their phytotherapeutic potencies. A total of twenty five (25) male albino rats were grouped randomly into groups. Group A (control), Group B (0.6g/100ml of water of HS extract) Group C (1.2g/100ml HS extract), Group D (1.8g/100ml HS extract) and Group E (1.8g/100ml + vitamin C) and treatment period was 28 days. The results indicate the presence of protein, carbohydrate, fat, fibre, ash, calcium, phosphorus, magnesium 4.40, vitamin C, dry matter and moisture content. Phytochemistry showed the presence of alkaloid, tannin, flavonoid, phenol, saponin. There was no significant difference in weight of the organs when compared with the control (Group A). There were inflammations on the liver tissues in Group B, C, D when compared with the control (Group A) and Group E. The kidney organs (Group A-E) showed no difference in their normal gross anatomical features, that is size, colour, and consistency. From the study, we may say that, consumption of the HS extract without substantial amount of ascorbic acid (vitamin C) caused changes on the histology of the liver, which suggest toxicity to the liver. The dose dependent or long term administration might have been too toxic to the rats. It can be concluded that taking HS in the absence of vitamin C is dangerous to health.

African Journal of Pharmacy and Pharmacology Effects of Hibiscus rosa-sinensis leaf products on haematological indices, lipid profile and hepatic parameters of hyperlipidemic rat

Previously, we demonstrated that Hibiscus rosa-sinensis leaf contains various secondary metabolites including saponins, tannins and oxalates, which are known to possess documented pharmacological activities. In this present study, we investigated the effects of the leaf products on haematological indices, lipid profile and hepatic parameters using Wistar albino rat bioassay. Data suggested that though the haemoglobin counts, packed cell volume and red blood count were not significantly (P > 0.05) affected by the leaf products, lipid profile tests results showed that the blood total cholesterol (TC) and low-density lipoprotein (LDL) of rats increased on feeding with high fatty diet (HFD). Administration of the leaf products dose-dependently resulted in significant decreases (P < 0.05) in the TC and LDL levels while the high-density lipoprotein level was further increased. Liver function test (LFT) showed no evidence of hepatotoxicity on the administration of the products as assayed liver enzymes and proteins did not vary between HFD administered animals, treated with or without leaf products. Comparatively, dried leaf products had more potent biological activities than the extracted leaf products. These findings suggested that H. rosa-sinensis leaves possess pharmacological potentials for treatment of metabolic syndrome related disease conditions.

Effects of methanolic calyx extract of Hibiscus sabdariffa on body weight, blood cholesterol and liver marker enzymes in Wistar rats

Journal of Medicinal Plants Research, 2018

Eighty male albino rats were used to investigate the effects of methanolic calyx extract of Hibiscus sabdariffa on body weight, blood cholesterol and liver enzymes markers. Twenty-eight days oral administration of 100, 200, 400 and 800 mg/kg body weight of the extract showed a significant (p<0.05) time-dependent decrease in the body weights of all the treated groups when compared with the control, with 100 mg/kg causing significant (p<0.05) decrease at weeks 2 and 4, respectively when compared with other treatment doses. The extract significantly (p>0.05) decreased the serum cholesterol and increased the liver marker enzymes (ALP, ALT and AST) in dose-dependent and time-dependent manner, when compared with the control. However, on day 21, the group treated with 400 mg/kg showed a significant (p<0.05) increase the serum cholesterol, and decrease in liver marker enzymes when compared with the rest of the treatment groups. Histopathology from all the treatment groups revealed graded degrees of vacuolar degeneration of the hepatocytes and peri-portal infiltration of mononuclear leucocytes. The results of this present study suggests that the methanolic calyx extract of H. sabdariffa possesses anti-obesity and hypocholesterolemic potentials which should be harnessed with caution due to its tendency to adversely affect the liver.

Effects of Hibiscus Sabdariffa Calyces Aqueous Extract on Serum Cholesterol, Body Weight and Liver Biomarkers of Rattus Novergicus

International Journal of Indigenous Medicinal Plants, ISSN:2051-4263, Vol.46, Issue.4, 1405 - 1411, 2013

The present study investigated the effect of Hibiscus sabdariffa calyx aqueous extract on the serum cholesterol, body weight and liver marker enzymes activities of normal albino rats. The aqueous extract was orally administered (100 – 800 mg/kg body weight) for 28 days to normal male albino rats. Total cholesterol, body weight, aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) levels were measured. Hibiscus sabdariffa administration significantly reduced serum cholesterol and body weight in a dose and duration dependent pattern. AST, ALP and ALT levels were significantly elevated in a dose and duration dependent pattern. The significant increase in the levels of the liver enzymes tends to suggest dysfunction in the coordinating of the liver activity. The extract ability to lower the total cholesterol level and body weight suggests its usefulness as a hypocholesterolemic and anti-obesity agent.

Journal of Medicinal Plants Research Effects of methanolic calyx extract of Hibiscus sabdariffa on body weight, blood cholesterol and liver marker enzymes in wistar rats

Eighty male albino rats were used to investigate the effects of methanolic calyx extract of Hibiscus sabdariffa on body weight, blood cholesterol and liver enzymes markers. Twenty-eight days oral administration of 100, 200, 400 and 800 mg/kg body weight of the extract showed a significant (p<0.05) time-dependent decrease in the body weights of all the treated groups when compared to the control, with 100 mg/kg causing significant (p < 0.05) decrease at week 2 and 4 respectively when compared to other treatment doses. The extract significantly (p>0.05) decreased the serum cholesterol and increased the liver marker enzymes (ALP, ALT & AST) in dose-dependent and time-dependent manner, when compared to the control. However, on day 21, the group treated with 400 mg/kg showed a significant (p<0.05) increase the serum cholesterol, and decrease in liver marker enzymes when compared to the rest of the treatment groups. Histopathology from all the treatment groups revealed graded degrees of vacuolar degeneration of the hepatocytes and peri-portal infiltration of mononuclear leucocytes. The results of this present study suggests that the methanolic calyx extract of Hibiscus sabdariffa possesses anti-obesity and hypocholesterolemic potentials which should be harnessed with caution due to its tendency to adversely affect the liver.

Effect of Ethanolic Extract of Hibiscus Cannabinus Leaf on High Cholesterol Diet Induced Obesity in Female Albino Rats

Asian Journal of Pharmaceutical and Clinical Research, 2013

Objective: Even with the growing awareness, about 3% of Indian population are obese and worldwide around 1.2 billion people suffer with obesity and those suffer with the related complications might be much higher. Preventing the occurrence of obesity by including certain food substances with antiobesity effect in our regular diet would always be a welcome move. In this context, the ethanolic extracts of Hibiscus cannabinus leaves were examined by in vivo study on high cholesterol diet induced obese female albino rats, since female obesity is prevalent among Indian women. Hibiscus cannabinus is a plant of Malvaceae family, native to southern Asia. Methods: Female wistar rats were divided into five groups. Clinical condition was induced by giving high cholesterol diet and treated with the ethanolic extract of Hibiscus cannabinus leaf. a very common green used in Indian diet. Results: In cholesterol diet induced animals treated with the extracts of H. cannabinus and lovastatin demonstrated a significant decrease in the serum cholesterol, triglycerides, LDL-C and also significant decrease in the levels of SGOT and SGPT activities when compared to cholesterol diet induced group. The groups treated with the extracts of H .cannabinus also showed decrease in body weights when compared to cholesterol induced group. The histopathological studies of liver sections of rats showed reversal of changes, and prevent the fat accumulation in the liver of hyperlipidemic group after treatment with H. cannabinus. Conclusion: Results suggested that the ethanolic extracts of H. cannabinus possess significant antiobesity effect which might me attributed by its rich phytochemical presence.