Effect of Ethanolic Extract of Hibiscus Cannabinus Leaf on High Cholesterol Diet Induced Obesity in Female Albino Rats (original) (raw)
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ABSTRACT Obesity is capturing the whole world very rapidly. Both medical community and common people are aware of the health risks associated with obesity. Different phytochemicals present in medicinal plant parts have antiobesity properties. The antiobesity properties of medicinal plants are exhibited by different mechanisms. There are different studies for exactly knowing the cause of animal obesity. A new scientific approach about the present situation of this disease may initiate additional research in the field to assess the contribution of phytochemicals in obesity and possibly to prevent or treat the widespread epidemic of obesity. This review will try to focus and summarize on the main findings available in the literature regarding the effect of different medicinal plants and their extracts on human obesity. In this paper we review the present knowledge in the field as this may be of importance to those who deal with research on antiobesity for the benefit of the common people. Keywords: Obesity, adipocytes medicinal plants, phytochemicals, hypolipidemia, hypercholesterolemia.
International Journal of Pharmacy and Pharmaceutical Sciences, 2019
Objective: It was investigated to enlighten the phytochemical screening and antihyperlipidemic activity of seeds of Canavalia ensiformis (L.) in High fat diet Streptozotocin-induced diabetic rats. Methods: The ethanolic seed extract of Canavalia ensiformis (EECE) at a dose of 400 mg/kg and 200 mg/kg was administered to diabetic rats. Simvastatin (4 mg/kg) is used as a standard drug. Results: The statistics were assessed by using one way ANOVA followed by Dunnet's multiple comparison tests. To unfold the mechanism we studied all the biochemical parameters like Total cholesterol (TC), Triglycerides (TG), high-density lipoproteins (HDL), low-density lipoproteins (LDL) and histopathological examination of Liver tissue section. The ethanolic extracts of seeds of Canavalia ensiformis showed significant reduce of the level of cholesterol, triglycerides, LDL, VLDL and a significant increase in the serum level of HDL at 400 mg/kg rather than 200 mg/kg. Conclusion: Further the characterization and isolation of the constituents could be done to know the exact mechanism of hyperlipidemic activity. Statistical analysis of this screening method conforms that the proposed method is appropriate and it can be useful, gives the basic idea to the researcher who is working in an area like Antihyperlipidemic activity.
Background: Hibiscus sabdariffa L. (Hs; Malvaceae) is commonly known as roselle or red sorrel in English or karkadè in Arabic. It is a tropical plant native to India and Malaysia. Roselle extracts have been widely reported to have beneficial health effects. The aim of this study was to determine the effective dose of Hs aqueous extract, which is a possible reducing agent in diet-induced obese rats, and monitoring any toxicological effect. Methods: Male Sprague Dawley rats (n= 24) aged 6-7 weeks and weighing 210 ± 3.5 g (mean ± S.E.M) were used. The rats were fed a high fat diet (HFD) for 8 consecutive weeks to induce obesity. The animals were then randomly assigned to one of five groups based on the concentration of Hs aqueous extract. The normal group (NG) received a normal diet (commercial chow) throughout the study. The obese group (ObG) included obese rats administered with tap water ad libitum. Groups 1 (150 mg/kg), 2 (200 mg/kg), 3 (250 mg/kg), and 4 (300 mg/kg) were obese rats continuously fed with HFD in combination with the Hs extract for 10 weeks. The effect of Hs on some haematological and blood biochemical parameters were also evaluated. Results: Hs aqueous extract is a rich source of anthocyanins. The main compounds detected are delphinidin-3-O-sambubioside and delphinidin-3-O-sambubioside. The extract also possesses high antioxidant properties which may be caused by these anthocyanins. The anti-obesity effect of Hs aqueous extract was demonstrated by the significant reduction in the weight gain and abdominal weight (p < 0.05) between treated and non-treated groups, which was dose-dependent. Overall, our study demonstrated that oral administration of Hs at doses of 150, 200, 250, and 300 mg/kg for ten weeks did not cause any toxicity effect within the obese rats. Conclusions: The results of the study implied Hs aqueous extract at 300 mg/kg is the dose which can the most weight reduction effect with no severe haematological and biochemical changes in all experimental animals. Keywords: Hibiscus sabdariffa, obese rats, roselle, aqueous extract, and body weight
Obesity is capturing the whole world very rapidly. Both medical community and common people are aware of the health risks associated with obesity. Different phytochemicals present in medicinal plant parts have antiobesity properties. The antiobesity properties of medicinal plants are exhibited by different mechanisms. There are different studies for exactly knowing the cause of animal obesity. A new scientific approach about the present situation of this disease may initiate additional research in the field to assess the contribution of phytochemicals in obesity and possibly to prevent or treat the widespread epidemic of obesity. This review will try to focus and summarize on the main findings available in the literature regarding the effect of different medicinal plants and their extracts on human obesity. In this paper we review the present knowledge in the field as this may be of importance to those who deal with research on antiobesity for the benefit of the common people. Obesi...
Effect of Hibiscus sabdariffa on obesity in MSG mice
Journal of Ethnopharmacology, 2007
The aim of the present investigation was determine whether a standardized Hibiscus sabdariffa calyces aqueous extract has an effect on body weight in an obese animal model induced by the administration of monosodium glutamate. Hibiscus sabdariffa aqueous extract, containing 33.64 mg of total anthocyanins per each 120 mg of extract, was orally administered (120 mg/kg/day) for 60 days to healthy and obese mice, and body weight gain, food and liquid intake, aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholesterol, and triglycerides levels were measured. Hibiscus sabdariffa administration significantly reduced body weight gain in obese mice and increased liquid intake in healthy and obese mice. ALT levels were significantly increased on the 15th and 45th days in obese mice, but AST levels did not show significant changes. Mortality was not observed in the Hibiscus sabdariffa treated groups. Triglycerides and cholesterol levels showed non-significant reductions in animals treated with Hibiscus sabdariffa. Our data confirm the anti-obesity effect of Hibiscus sabdariffa reported by the Mexican population.
International Journal of Basic & Clinical Pharmacology, 2019
Background: Hibiscus sabdariffa L. or Roselle, is one of the most common flower plants cultivated worldwide. Traditionally, it is claimed to reduce weight and cholesterol level. The aim of this study was to evaluate the effects of anthocyanin-rich Roselle aqueous extract on the physical, biochemical and histological changes in obese-hypercholesterolaemic rat model.Methods: Thirty Sprague-Dawley male rats were divided into five groups (n=6 per group). Group 1 is normal control (NC) rat that was fed with normal diet. The remaining rats (Group 2-5) were fed with commercial high-fat diet (HFD) for 6 weeks to induce obese-hypercholesterolaemic (OH) rat. After induction, the OH rats were divided into 4 groups: OH-Untreated group, OH-ARTE; OH treated with 300 mg/kg Roselle aqueous (anthocyanin-rich) and 1% trifluoroacetic acid extract (ARTE), OH-Orlistat; OH treated with 37 mg/kg orlistat and OH-Atorvastatin; OH treated with 10 mg/kg atorvastatin. After the 3 weeks intervention period, all...
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The location and quantity of body fat determine the health risks, limiting people with obesity. Recently, polyphenols have attracted the attention of the scientific community because of their potential use for the reduction of obesity. A proprietary formula comprised of a blend of Lippia citriodora and Hibiscus sabdariffa has been recognized for its high content of polyphenols, powerful antioxidant molecules that may prevent weight gain and could be helpful for the treatment of obesity, as proven previously by in vivo models. The aim of the present study is to determine if the supplementation with Lippia citriodora and Hibiscus sabdariffa is useful for the treatment of obesity and/or weight control in subjects without a controlled diet. The intake of the extract for 84 days reduced body weight, the body mass index, and the fat mass measured with both bioimpedance and densitometry. This decrease in fat mass was observed to a greater extent, being significant, in the fat mass of the t...
Effect ofHibiscus sabdariffaextract on high fat diet–induced obesity and liver damage in hamsters
Food & Nutrition Research, 2015
Background: Obesity is a chronic metabolic disorder associated with an increase in adipogenesis and often accompanied with fatty liver disease. Objective: In this study, we investigated the anti-obesity effects of Hibiscus sabdariffa water extract (HSE) in vivo. Method: Eight-weeks-old male mice were divided into six groups (n 08 per group) and were fed either normal feed, a high fat diet (HFD), HFD supplemented with different concentrations of HSE, or HFD supplemented with anthocyanin. After 10 weeks of feeding, all the blood and livers were collected for further analysis. Results: Mesocricetus auratus hamster fed with a high-fat diet developed symptoms of obesity, as determined from their body weight change and from their plasma lipid levels. Meanwhile, HSE treatment reduced fat accumulation in the livers of hamsters fed with HFD in a concentration-dependent manner. Administration of HSE reduced the levels of liver cholesterol and triglycerides, which were elevated by HFD. Analysis of the effect of HSE on paraoxonase 1, an antioxidant liver enzyme, revealed that HSE potentially regulates lipid peroxides and protects organs from oxidation-associated damage. The markers of liver damage such as serum alanine aminotransferase and aspartate aminotransferase levels that were elevated by HFD were also reduced on HSE treatment. The effects of HSE were as effective as treatment with anthocyanin; therefore the anthocyanins present in the HSE may play a crucial role in the protection established against HFD-induced obesity. Conclusions: In conclusion HSE administration constitutes an effective and viable treatment strategy against the development and consequences of obesity.
Indian Journal of Pharmaceutical Education and Research, 2019
Objective: To investigate the effect of hydro-ethanolic fraction of leaves of M. longifolia (J. Koenig) on high fat diet induced metabolic disorder. Methods: The M. longifolia extract was obtained from the successive extraction followed by fractionation. Ex vivo glucose uptake study was performed. Body weight and blood glucose was checked at regular intervals. After euthanasia serum biochemical parameters, lipid parameters were estimated. Organs were isolated, weighed and fixed in formalin for histopathological analysis. Results: Compared to control, M. longifolia leaf fractions showed significant glucose uptake inhibition in ex vivo studies (rat hemi diaphragm and rat intestinal glucose uptake assay). HFD group showed significant weight increase as compared to normal control. Blood glucose level and glycosylated haemoglobin was found elevated in disease group (obesity and diabetic group). Significant increase in lipid profile of disease group aggravates the diabetic condition. Animal treated with M. longifolia leaf fractions (400mg/kg) helps in normalisation of liver and white adipose tissue. Conclusion: Metabolic syndrome is an agglomeration of disorders inclusive of increased blood glucose, excess body fat, anomalous triglyceride and cholesterol level, which on joint occurrence leads to diabetes and increased risk of heart disease. Our data suggest that treatment with M. longifolia to HFD + STZ group alleviates the diabetes and helps in control of obesity. It may be due to presence of various bioactive constituents.
The Effect of Extract of Canarian Nuts on Reduction of Total Cholesterol Levels of Hyperglicemic Rat
International Journal Papier Advance and Scientific Review
This study aims to determine the effect of walnut extract (Canarium indica) on the total cholesterol levels of hyperglycemic Wistar rats (Rattus norvegicus L). Experimental research with pre-post-test control design. The samples in this study were 28 rats induced by alloxan to become hyperglycemic. The test animals were divided into four groups, each consisting of seven: negative control (Na CMC 1%), positive control (metformin 150 mg / Kg BW), walnut extract group 300 mg / Kg BW (0.06g / 200gr BW), and extract 600 mg / Kg BW (0.12 / 200g BW). The intervention was given walnut extract for 21 days. Total cholesterol levels were measured by the Easytouch Multi-Monitoring System. This study used SPSS with paired t-test and ANOVA test. The results showed a significant reduction in cholesterol levels in all groups after treatment (p <0.05). There was a significant difference in the reduction of total cholesterol levels between groups with a value of p = 0.037. The results of the post...