Determination of Water-and Fat-Soluble Vitamins by HPLC By Thermo Dionex (original) (raw)
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Analysis of Water-Soluble Vitamins in Supplement Mixtures by HPLC/Uv
The present study describes HPLC/UV method with varying conditions (mobile phases content, flow rates, solvents) for quantitation of water-soluble vitamins (WSV) in supplement mixtures. The method was optimized in respects of analytical and chromatographic parameters such as retention time, symmetry factors, column efficiency as number of theoretical plates, capacity factors, specificity, repeatability, LoD, LoQ and linearity. The performance of these method distinguished with excellent application in the assay tests of WSVs in supplement mixtures containing Thiamine, Riboflavine, Pyridoxine, Niacinamide and Folic acid.
Modern analytical methodologies in fat and water soluble vitamins
John Wiley and Sons eBooks, 2000
Michael F. Holick 2.1. Origins of Vitamin D 2 and Vitamin D 3 2.2. Metabolism and Biologic Functions of Vitamin D 2.3. Measurement of Vitamin D Bioactivity 2.4. Modern Methods for Determining the Vitamin D Content in Blood 2.5. Strategies for Determining Circulating Concentrations of 25-Hydroxyvitamin D 2.6. Strategies for Determining Circulating Concentrations of 1,25-Dihydroxyvitamin D 2.7. Measurement of 24,25-Dihydroxyvitamin D and 25,26-Dihydroxyvitamin D 2.8. Clinical Utility for Assays for Vitamin D and Its Metabolites 2.8.1. Vitamin D 2.8.2. 25-Hydroxyvitamin D 2.8.3. 1,25-Dihydroxyvitamin D 2.8.4. Other Metabolites 2.9. Conclusion References
Química Nova, 2013
In the present study, a reversed-phase high-performance liquid chromatographic (RP-HPLC) procedure was developed and validated for the simultaneous determination of seven water-soluble vitamins (thiamine, riboflavin, niacin, cyanocobalamin, ascorbic acid, folic acid, and p-aminobenzoic acid) and four fat-soluble vitamins (retinol acetate, cholecalciferol, α-tocopherol, and phytonadione) in multivitamin tablets. The linearity of the method was excellent (R 2 > 0.999) over the concentration range of 10-500 ng mL −1. The statistical evaluation of the method was carried out by performing the intra-and inter-day precision. The accuracy of the method was tested by measuring the average recovery; values ranged between 87.4% and 98.5% and were acceptable quantitative results that corresponded with the label claims.
Journal of Chromatography A, 2000
In the present work, a reversed-phase high-performance liquid chromatographic procedure has been developed for the determination of water-soluble vitamins (thiamine hydrochloride, pyridoxine hydrochloride, nicotinamide, riboflavin phosphoric ester and cyanocobalamine) and fat-soluble vitamins (retinol palmitate, cholecalciferol, a-tocopherol acetate) in multi-vitamin pharmaceutical formulations. The sample treatment proposed consists of a solid-phase extraction with C AR 18 cartridges that allow the separation of fat-soluble vitamins, which were retained on the sorbent, from water-soluble vitamins. Afterwards, the water-soluble vitamins were analysed by HPLC on a Nova-Pack C (15033.9 mm, 4 mm) analytical 18 column, using CH OH-0.05 M CH COONH as mobile phase The chromatographic analysis of the fat-soluble vitamins 3 3 4 was carried out after their sequential elution with methanol and chloroform from C sorbent, on the above column. The 18 21 mobile phase employed was MeOH-CH CN (95:5, v / v) working at a flow-rate of 2 ml min in isocratic mode. The 3 solid-phase extraction for these vitamins had been previously optimised. The experimental variables studied were: application volume, elution solvents and cleaning solutions. The UV-Vis detection of vitamins was made at 270 nm for all the water-soluble vitamins (362 nm for B) and 285 nm for the water-soluble and fat-soluble vitamins present in real 12 samples at different concentration levels. The accuracy of the method was tested obtaining an average recovery ranging between 78 and 116%.
ChemInform, 2005
High-performance liquid chromatographic methods are the most often used methods for the determination of water-soluble vitamins (WSV) and fat-soluble vitamins (FSV). General approaches in quantification, occurring forms of vitamins, influences, which can affect stability of vitamins, necessary precautions in sample handling, pre-run sample stabilization, extractions procedures, and HPLC quantifications are mentioned and compared. This paper provides basic guidance for using HPLC in analysis of WSV and FSV. Finally, some methods for the quantification of WSV and FSV in pharmaceutical preparations, food supplements, and biological samples are reviewed.
DEVELOPMENT AND VALIDATION OF NEW RP-HPLC METHODS FOR STABILITY STUDY OF FAT SOLUBLE VITAMINS
International Journal of Pharmacy and Pharmaceutical Sciences, 2013
Objective-A simple reversed phase HPLC method was developed for the determination of Vitamin A and Vitamin E present in bulk and pharmaceutical dosage forms. Methods-An Alltech Prevail-C18 150 X 4.6 mm, 5 µm column with mobile phase Acetonitrile: Methanol (75:25) was used. The flow rate was 1.0 ml/min and effluent was monitored at 220 nm. Results-The retention times were 3.41 min and 8.74 min for vit.A and vit.E respectively. The linearity range was found to be 1-200 g/ml for vit.A and 1-500 g/ml for vit.E respectively. Conclusion-The proposed method was validated for linearity, precision and accuracy. The stability of these vitamins was studied for the period of two month.
Committee on food nutrition. Water-soluble vitamins
Journal of AOAC International
Simultaneous Determination of Vitamins.-Klejdus et al. (1) described a simultaneous determination of 10 waterand 10 fat-soluble vitamins in pharmaceutical preparations by liquid chromatography-diode-array detection (LC-DAD). A combined isocratic and linear gradient allowed separation of vitamins in 3 distinct groups: polar, low-polar, and nonpolar. The method was applied to pharmaceutical preparations, fortified powdered drinks, and food samples, for which results were in good agreement with values claimed. Heudi et al. (2) described a separation of 9 water-soluble vitamins by LC-UV. The method was applied for the quantification of vitamins in polyvitaminated premixes used for the fortification of infant nutrition products. The repeatability of the method was evaluated at different concentration levels and coefficients of variation were <6.5%. The concentrations of vitamins found in premixes with the method were comparable to the values declared. A disadvantage of the methods mentioned above is that sample composition has to be known in advance. According to European legislation, for example, foods might be fortified with riboflavin phosphate or thiamin phosphate, vitamers which are not included in the simultaneous separations described. Vitamin B 2 .-Viñas et al. (3) elaborated an LC analysis of riboflavin vitamers in foods. Vitamin B 2 can be found in nature as the free riboflavin, but in most biological materials it occurs predominantly in the form of 2 coenzymes, flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD). Several methods usually involve the conversion of these coenzymes into free riboflavin before quantification of total riboflavin. According to the authors, there is growing interest to know flavin composition of foods. The described method separates the individual vitamers isocratically. Accuracy of the method is tested with 2 certified reference materials (CRMs). Vitamin B 5 .-Methods for the determination of vitamin B 5 in foods are limited because of their low sensitivity and poor selectivity. Pakin et al. (4) proposed a post-column derivatization of pantothenic acid as a fluorescent compound and used this principle in a specific and sensitive method for the determination of free and bound pantothenic acid in a large variety of foods. A French laboratory invited European Year of interlaboratory test
Egyptian Journal of Chemistry, 2019
T HE purpose of this study is the development of analytical method for simultaneous determination of water-soluble vitamins (B 1 , B 2 , B 3 , B 6 , B 9 , B 12 and vitamin C) in dietary supplement syrup, multivitamins and mineral dietary supplement syrup with iron, dietary supplement capsules and balanced supplemental nutrition for children and adult gum by using gradient RP-HPLC methods with PDA detector andcalculating the uncertainty for each matrix of analytical results based on the information from the validation process. The separation of water-soluble vitamins was performed on waters Spherisorb ODS2 (250 mm×4.6mm, 5μm) column. The wavelength was 272 nm at room temperature and the detection limits ranged from 2.4 to 8.3 ng/µl. The average recovery was 98%-102 % and correlation coefficient (r) was 0.9997 to 1.
Journal of Chromatography B-analytical Technologies in The Biomedical and Life Sciences, 2004
In the present study, a simple and rapid reversed-phase HPLC procedure has been developed for the simultaneous determination of eight fat-soluble vitamins (retinol, menadione, menaquinone, δ-tocopherol, cholecalciferol, α-tocopherol, α-tocopherol acetate and phylloquinone) in biological fluids: blood serum and urine. The analytical column, Phenomenex Luna C18 (150mm×4.6mm) 3μm, was operating at ambient temperature. Mobile phase consisted of a mixture of CH3OH–CH3CN