Antimicrobial, insecticidal and phytotoxic activities of Indigofera heterantha roots (original) (raw)
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Phytochemical and Biological screening of the seeds of Indigofera heterantha wall
Indigofera heterantha seeds are used in folk medicine for the treatment of gastrointestinal disorder and abdominal pain. Phytochemical and biological Screening on the seeds extracts were carried out. Alkaloids, steroids, terpenoids, flavonoids, anthraquinones, tannins, phlobatanins, saponins, glycosides and reducing sugars were detected in the extract. The antibacterial activity tested against Escherichia coli, Bacillus Subtilis, Shigella flexenari, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi were negative. The Brine shrimp and insecticidal activity against Tribolium castaneum, Rhyzopertha dominica and Callosbruchuanalis were found negative at the concentration of 1019.10 µg/cm .
phytochemical invitigation of seeds of indigofera heterantha
Indigofera heterantha seeds are used in folk medicine for the treatment of gastrointestinal disorder and abdominal pain. Phytochemical and biological Screening on the seeds extracts were carried out. Alkaloids, steroids, terpenoids, flavonoids, anthraquinones, tannins, phlobatanins, saponins, glycosides and reducing sugars were detected in the extract. The antibacterial activity tested against Escherichia coli, Bacillus Subtilis, Shigella flexenari, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi were negative. The Brine shrimp and insecticidal activity against Tribolium castaneum, Rhyzopertha dominica and Callosbruchuanalis were found negative at the concentration of 1019.10 µg/cm . 2
Journal of Enzyme Inhibition and Medicinal Chemistry, 2009
The antibacterial, antifungal, acute cytotoxicity, phytotoxicity and insecticidal profile of the crude extract and various fractions of Indigofera gerardiana have been studied. Six bacterial and fungal strains were used, of which Samonella typhi and Microsporum canis were the most susceptible strains with MICs 0.37 mg/mL and 0.09 mg/mL, respectively. The crude extract and the fractions showed low insecticidal activity against Sitophilus oryzae, Rhyzopertha dominica and Callosbruchus analis but no activity against Tribolium castaneum. The brine shrimp lethality assay showed absence of any measurable cytotoxicity of the crude extract and fractions, showing a good safety profile at a preliminary level. All the fractions except crude extract revealed profound and highly significant herbicidal activity against Lemna minor at the concentration of 1000 mg/mL. Indigofera gerardiana was shown by in-vitro assays to be a potential source for natural antifungal, antibacterial and herbicidal agents.
Antimicrobial efficacy and phytochemical analysis of Indigofera trita Linn
African Journal of Traditional, Complementary and Alternative Medicines, 2013
An in vitro antimicrobial activity and phytochemical analysis of various extracts of Indigofera trita L. viz. petroleum ether, chloroform, acetone, ethanol and aqueous extracts were carried out. A total of 21 microorganisms (19 bacteria and 2 fungal strains) were used for antimicrobial activity by disc diffusion method and a standard procedure was used to identify the phytochemical constituents. Petroleum ether extract showed moderate inhibitory activity against Staphylococcus aureus (14.40 mm),
Antimicrobial efficacy and phytochemical analysis of Indigofera trita Linn
African Journal of Traditional, Complementary and Alternative Medicines, 2013
An in vitro antimicrobial activity and phytochemical analysis of various extracts of Indigofera trita L. viz. petroleum ether, chloroform, acetone, ethanol and aqueous extracts were carried out. A total of 21 microorganisms (19 bacteria and 2 fungal strains) were used for antimicrobial activity by disc diffusion method and a standard procedure was used to identify the phytochemical constituents. Petroleum ether extract showed moderate inhibitory activity against Staphylococcus aureus (14.40 mm),
Phytochemical and cytotoxicity testing of Indigofera lupatana Baker F.
Indigenous plants are promising as a cheap and alternative complimentary medicine (CAM). Although the use of phytomedicine has been in practice for long, little has been done to evaluate their effectiveness, safety, target organisms and also their chemical characterization. Indigofera lupatanaBaker F. (locally known as Mugiti) has been used by Mbeere community of Kenya to treat many conditions suchas cough, diarrhea, pleurisy and gonorrhea. In this study, powdered sample of I. lupatanaBaker F. roots were sequentially extracted using hexane, ethyl acetate, dichloromethane and methanol solvents. The resultant extract fractions were subjected to phytochemical tests and brine shrimp cytotoxicity bioassay. Results showed that the fractions had Phenolics, Flavonoids, Tannins, Saponins, Terpenoids, Cardiac glycosides, Steroids and Phlobatannins. These are responsible for the bioactivity of the sample fractions.All fractions had a LC50value greater than 1000µg/ml which is an indication that they are all non toxic. Therefore Indigofera lupatanaBaker F. can be used as an alternative source of new, effective, safe drug(s) because it demonstrated presence of phytochemicals with no oral toxicity.
Phytochemical, antimicrobial and cytotoxic evaluation of Indigofera serpentinicola
Bangladesh Journal of Pharmacology, 2015
Methanol and aqueous root extracts of Indigofera serpentinicola were investigated for their phytochemical, antimicrobial and cytotoxic properties. Phytochemical analysis showed the presence of alkaloids, saponins, tanins, terpenoids, cardiac glycosides, phenols, reducing sugars oils and fats in both extracts. Flavanoids were only detected in the methanolic extract. Antimicrobial activity was evaluated using the Kirby-Bauer disk diffusion method. The extracts showed low activity against Staphylococus aureus, Cornybacterium diptheriae, Pseudomonas aeruginosa and Salmonella enterica and no activity against Escherichia coli. The diameters zones of inhibition ranged between 3-10 mm. The aqueous extract had higher activity showing zones of inhibition of 10 mm against S. enterica. Brine shrimp lethality test showed LC50 values which ranged from 0.079-0.158 mg/mL, showing that the extracts were highly toxic.
ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS OF INDIGOFERA ASPALATHOIDES VAHL. EX. DC.
Plants are rich in a wide variety of secondary metabolites such as tannins, terpenoids, alkaloids and flavonoids which have been found in-vitro to have antimicrobial properties. The present study was conducted with a view to evaluate the therapeutic potentials Indigofera aspalathoides plant extracts against human pathogen. The leaves and aerial parts of Indigofera aspalathoides were dried and powdered leaf and stem material was extracted by soxhlation method using a non-polar (Hexane) solvent and polar solvents (Chloroform, Ethanol and Water), respectively and extracts were then subjected to phytochemical screening. Extract of Indigofera aspalathoides was evaluated for its antimicrobial activity against a wide variety of pathogenic bacteria such Escherichia coli, Klebsiella pneumonia and Aeromonas hydrophila by disc diffusion method. In the extract of I.aspalathoides maximum yield was obtained from in the leaf ethanol (20.64±1.02) than the chloroform (15.80±3.28) and hexane (6.52±1.78) and compare the stem extracts ethanol(10.23±3.55) was in high followed by chloroform(7.28±2.36) and hexane (3.52±1.35). Phytochemical analysis showed the presence of various phytoconstituents. Alkaloids, Phenolic compounds, Flavonoids, Tannins and Terpenoids were found to be present in all extracts of leaf and stem. Reducing sugar was absent both leaf and stem. Pholobatanins recorded in leaf and absent were in stem.Compare the solvent extracts of leaf, crude ethanol extracts of I. aspalathoides leaf caused more antibacterial activity against pathogens E. coli (24.4±2.5), B. subtilis (23.3±2.4) and A. hydrophila (20.6±3.1) followed by chloroform against E. coli (18.2±1.5) and hexane against A. hydrophila (11.2±1.2). Compare the stem extracts; hexane extracts caused more antibacterial activity against K. pneumonia (29.2±2.9) followed by chloroform against B. subtilis (25.2±0.4) and against B. subtilis (12.7±0.8). The results support that these plant extracts can be used for the treatment of bacterial diseases.