Biliary secretion in conscious rabbits: surgical technique (original) (raw)
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Quarterly Journal of Experimental Physiology, 1981
The role of enterohepatic circulation of bile salts in biliary secretion in conscious rabbits has been investigated before and after cholecystectomy. Bile flow was higher and bile salt concentration lower in cholecystectomized than in intact rabbits: this could have been caused by a negative feedback effect on bile salt synthesis as the circulating bile salt pool increased. The effects of cholecystectomy on flow and bile salt concentration balanced each other. Bile flow and bile salt concentration declined after interruption of the enterohepatic circulation in both cholecystectomized and intact rabbits. Furthermore, the percentage of the flow of bile independent of bile salt secretion increased, while that independent of total analysed solutes decreased after the enterohepatic circulation was broken. These results confirm that the decrease in bile flow after interruption of the enterohepatic circulation is due to loss of bile salts and not of electrolytes. * See Methods.
Quarterly Journal of Experimental Physiology, 1981
The biliary response to feeding has been investigated in intact and cholecystectomized rabbits, with and without interruption of the enterohepatic circulation of bile salts. The relative contribution of the different secretory mechanisms involved in this process has been examined. Feeding induced an increase in the flow and bicarbonate concentration of the bile and a slight decrease in the bile salt content in rabbits with gall bladders and the enterohepatic circulation intact. Feeding with the enterohepatic circulation interrupted elicited a drop in bile flow and the bile salt concentration, but an increase in bicarbonate concentration. Interruption of the enterohepatic circulation thus dramatically modifies the feeding response: a response to food is still detectable after interruption, but is masked by the negative effects of drainage of the bile away from the duodenum.
Use of a bidirectional cannula to study biliary secretion in conscious dogs
Laboratory Animals, 1983
Summary A new biliary bidirectional cannula is described which allows the study of biliary secretion in conscious dogs under conditions which approach physiological normality. Materials and methods Experiments were carried out on 9 adult dogs (body weight 20-25 kg). The animals were fasted for 48 h prior to surgery. Drinking water was given ad libitum. Since the first report of
Basal biliary secretion in conscious chicken and role of enterohepatic circulation
Comparative Biochemistry and Physiology Part A: Physiology, 1981
l. Enterohepatic circulation of bile salt role. its interruption effects, gallbladder contribution and importance of some electrolytes, on total biliary secretion of chicken have been investigated. 2. The gallbladder, in spite of its anatomical disposition, behaves exactly like that of mammals. When the vesicular duct is tied there is a retrograde flow of bile from gallbladder, although it causes no change in the results if a cholecystokinetic stimulus is not introduced. 3. The role of the enterohepatic circulation is important in this species. The results leave out electrolyte loss and indicate bile salt concentration decay as cause of hyposecretion after bile drainage, which agrees with lack of change of flow independent of bile salt. 4. Recovery of all parameters when bile is placed in duodenum shows that the observed effects are genuine and that animals were in good physiological state.
Radiologia Brasileira, 2013
Objective: To compare, by means of ultrasonography, pre-and postoperative anatomical changes arising from experimentally induced obstructive jaundice in porcine models. Materials and Methods: Six 36-day-old Landrace pigs underwent laparoscopically induced complete biliary obstruction by common bile duct ligation. Results: No difficulty was faced during the procedures and the surgical recovery was uneventful. After seven days, the animals showed jaundice, bilirubinuria and acholic stools. Comparative ultrasonography allowed visualization of hepatomegaly, cholecystomegaly and increased caliber of the common bile duct in all the animals, as well as changes resulting from cholestasis. The morphometric analysis revealed a significant increase in diameter of the gallbladders and left lateral liver lobes. Conclusion: Pigs represent appropriate experimental models for investigation of obstructive jaundice, and ultrasonography has shown to be sensitive, playing a relevant role in the diagnosis of extrahepatic biliary obstruction in such animals.
Manometric changes during retrograde biliary infusion in mice
American Journal of Physiology-Gastrointestinal and Liver Physiology, 2000
The manometric, ultrastructural, radiographic, and physiological consequences of retrograde biliary infusion were determined in normostatic and cholestatic mice. Intraluminal biliary pressure changed as a function of infusion volume, rate, and viscosity. Higher rates of constant infusion resulted in higher peak intraluminal biliary pressures. The pattern of pressure changes observed was consistent with biliary ductular and/or canalicular filling followed by leakage at a threshold pressure. Retrograde infusion with significant elevations in pressure led to paracellular leakage of lanthanum chloride, radiopaque dye, and [14C]sucrose with rapid systemic redistribution via sinusoidal and subsequent hepatic venous drainage. Chronic extrahepatic bile duct obstruction resulted in significantly smaller peak intrabiliary pressures and lower levels of paracellular leakage. These findings indicate that under both normostatic and cholestatic conditions elevated intrabiliary volumes/pressures re...
SECT ION 1 Anatomy , pathophysiology , and epidemiology of the biliary system
2006
Intrahepatic bile ducts There are more than 2 km of bile ductules and ducts in the adult human liver. These structures are far from being inert channels, and are capable of signifi cantly modifying biliary fl ow and composition in response to hormonal secretion. Bile secretion starts at the level of the bile canaliculus, the smallest branch of the biliary tree [6]. They form a meshwork between hepatocytes with many anastomotic interconnec