Effect of aqueous extract of Melastoma malabathricum leaves against paracetamol-and CCl4-induced liver toxicity in rats (original) (raw)
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Hepatoprotective Activity of Methanol Extract of Melastoma malabathricum Leaf in Rats
Journal of Acupuncture and Meridian Studies, 2013
The present study aimed to determine the hepatoprotective activity of a methanol extract of Melastoma malabathricum leaves (MEMM) using two established rat models. Ten groups of rats (n Z 6) were given a once-daily administration of 10% dimethyl sulfoxide (negative control), 200 mg/kg silymarin (positive control), or MEMM (50, 250, or 500 mg/kg) for 7 days followed by induction of hepatotoxicity either using paracetamol or carbon tetrachloride. Blood samples and livers were collected for biochemical and microscopic analysis. Based on the results obtained, MEMM exhibited a significant (p < 0.05) hepatoprotective activity against both inducers, as indicated by an improvement in the liver function test. These observations were supported by the histologic findings. In conclusion, M. malabathricum leaves possessed hepatoprotective activity, which could be linked to their phytochemical constituents and antioxidant activity; this therefore requires further in-depth studies.
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: The initial study was to screen the phytochemical content of methanol extract of M. malabathricum from seven different locations. The other objective was to evaluate the total phenolic content (TPC), Total flavonoid content (TFC), antioxidant potential and cytotoxic activity (on Hepatoma G2 cells) of these extracts and to determine the relationship between TPC and other parameters. Methods: The preliminary phytochemical screening for the presence of the secondary metabolite was carried out according to standard procedures. The TPC, TFC and antioxidant activity were determined using Folin-Ciocalteu method, aluminium chloride (colorimetric) methodand1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity respectively. Results: Qualitative phytochemical screening showed the presence of tannins, analkaloid, steroids, flavonoid, phenols, terpenoids and fixed oil but tested negative for the presence of glycoside and saponins. The samples were found to have high TPC and antioxidant activity. The Bachok Kelantan sample (L7) showed highest phenolic content (671.51±50.07 mg of GAE/g) as well as highest DPPH free radical scavenging activity (80.81% and IC50 102 µg/ml). The highest cytotoxic activity against HepG2 cells (IC50 1.4 µg/ml) was shown by KualaTerengganu, Terengganu sample (L1). The Spearman correlation showed that, there is a strong positive correlation between TPC and antioxidant activity (r = 0.714) as well as strong negative correlation between MTT IC50 Conclusion: The phenolic compounds are associated with the cytotoxic and antioxidant activities of M. malabathricum, whereas flavonoids are poorly and weakly associated with cytotoxic and antioxidant activities of M. malabathricum respectively. The total phenolic content, mean flavonoid content and mean antioxidant activity of M. malabathricum from different locations were significantly different across seven locations (p<0.05). and TPC (R =-0.649) of M. malabathricum from different locations. Moreover, there is a weak positive correlation between TFC and antioxidant activity (R = 0.286, p= 0.535). Also, there is poor correlation between TFC and cytotoxicity (R =-0.216, p= 0.64).
Drug Invention Today, 2013
Objective: To investigate the hepatoprotective activity of aqueous and methanol extracts of leaves of Morinda tinctoria Roxb. against paracetamol induced liver damage into rats. Methods: The hepatoprotective activity aimed for plant extracts was investigated for paracetamol induced hepatotoxicity into rats. SpragueeDawley rats of either sex were divided into 7 groups of 5 animals each and are given orally the following treatment for 10 days. The normal control was given 1% CMC 1 ml/kg b.w., p.o. Paracetamol at dose of 3 g/kg b.w., p.o. was given as toxic dose for inducing hepatotoxicity. Liv.52 (50 mg/animal, p.o) was given as reference standard. Two different doses of M. tinctoria extracts of both aqueous and methanol (100 mg/kg, p.o, 150 mg/kg, p.o) was tested for hepatoprotective activity. The treatment was given for 10 days and after 48 h of last treatment blood was collected from direct cardiac puncture and analysed for various serum parameter like serum glutamic pyruvic transaminase (SGPT), Serum glutamic oxaloacetic transaminase (SGOT), Total Bilirubin (TB), Direct Bilirubin and Total cholesterol (TC) in different groups. Results: The phytochemical investigation of the both extracts showed the presence of alkaloids, flavonoids, glycosides, carbohydrates, saponin and tannin and phenols. The paracetamol intoxication lead to histological and biochemical deterioration. The treatment with both aqueous and methanolic leaves extracts of M. tinctoria reduced the level of SGOT, SGPT, TB, DB and TC and also reversed the hepatic damage towards normal which further supports the hepatoprotective activity of leaf extracts of M. tinctoria. Conclusions: Both aqueous and methanol extracts of leaves of M.tinctoria have significant effect at higher dose of 150mg/kg.b.w.
2012
Melastoma malabathricum (MM) is a well-known plant in Malaysian traditional medicine, locally known as senduduk. Its ethanol and aqueous extracts have been used in the present investigation to study the immunomodulatory role on human peripheral blood mononuclear cell (PBMC), and the DPPH, ABTS and FRAP free radical scavenging activities were also measured. Total flavonoids and total phenolic contents were assayed and the antibacterial effect was tested against four species of bacteria; two Gram-positive (Staphylococcus aureus and Streptococcus agalactiae) and two Gram-negative (Escherichia coli and Klebsilla pneumonia). The tests were carried out using the disc diffusion, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods. Moreover, the acute toxicity was evaluated in vivo on the ethanol extract of MM to establish its safety when administered orally. In our results, both extracts of MM showed abilities to scavenge DPPH and ABTS free radicals, IC50 values: (11.599 +/- 0.84, 10.573 +/- 0.58 mu mol/L) and (62.657 +/- 0.78, 63.939 +/- 0.48 mu mol/L) for ethanol and aqueous extracts respectively. Indeed the ethanol extract evidenced high phenolic content (384.33 +/- 0.005 mg/g), flavonoids contents (85.8 +/- 0.009 mg/g) and ferric reducing antioxidant power (33,590 +/- 0.038 mmol/g), with high activity against S. aureus and S. agalactiae (11 +/- 0.3 and 12 +/- 0.6 mm inhibition zones). Likewise, the percentage of peripheral blood mononuclear cells (PBMC) viability was increased in response to MM, IC50 values (1.781 +/- 1.2 and 6.545 +/- 0.93 mu g/mL) for ethanol and aqueous extracts, respectively. In addition, our results showed that the MM extract is safe even at a high dose of 5,000 mg/kg and has no oral toxicity. These findings suggest the excellent medicinal bioactivity of MM and explain the popularity of this plant in the folk medicine as a remedy for different illnesses.
Research Article HEPATOPROTECTIVE EFFECT OF WHOLE PLANT EXTRACT FRACTIONS OF
2013
Objective: The objective of the study is to separate and identify the most effective hapatoprotective fraction of methanolic extract of Marsilea minuta (MMME) by fractionating and evaluating its fractions for hapatoprotective activity in three mechanistically devised models viz., CCl4, paracetamol and ethanol induced liver damage in rats. Methods: Acute toxicity study was carried out to the fractions according to the organization for economic corporation development (OECD)-420 guidelines. Liver damage was induced in different groups of rats by administering 1:1v/v CCl4 in olive oil 1ml/kg.b.w.p.o, 3g/kg.b.w.p.o paracetamol and 5g/kg.b.w.p.o ethanol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters, histology of liver of rats and the most eff ective bioactive fraction was screened for its effect on hepatic microsomal drug metabolizing enzymes (MDMA) and prothrombin time (PT). It was also tested for its antioxidant proper...
The aim of the study is to investigate the phytochemicals and hepatoprotective activity of Melia azedarach L leaves extracts against carbon tetrachloride (CCl 4) induced hepatotoxicity. The phytochemical screening was carried on the leaves extracts of Melia azedarach revealed the presence of some active ingredients such as Alkaloids, Tannins, Saponins, Phenols, glycosides, steroids, terpenoids and flavonoids. Leaves of Melia azedarach was successively extracted with ethanol against carbon tetrachloride (CCl 4) induced hepatotoxicity using Standard drug Silymarin (25 mg/kg). There was a significant changes in biochemical parameters (increases in serum glutamate pyruvate transaminase (SGPT), Serum glutamate oxaloacetate transaminase (SGOT), alanine phosphatase (ALP),serum bilirubin and decrease the total proteins content.) in CCl4 treated rats, which were restored towards normalization in Melia azedarach (500 mg/kg) treated animals. Thus the present study ascertains that the leaf extract of Melia azedarach possesses significant hepatoprotective activity.