Isolation and characterisation of Mongolian plant and soil actinomycete strains and their antibacterial compounds (original) (raw)
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A Comparison of Antibacterial Potential of Different Soil Actinomycetes from Pokhara
Prithvi Journal of Research and Innovation, 2021
Actinomycetes are widely distributed in the environment and used for the production of several important secondary metabolites like antibiotics, immunosuppressive agents, enzymes and antitumor agents. Therefore, the main objective of this study is to isolate and assess antibacterial potential of different actinomycetes obtained from different soil samples. This study was conducted in the microbiology laboratories of Prithvi Narayan Campus and Lambda Food Lab Pvt Ltd, Pokhara. A total of nine soil samples were collected from different places of Pokhara (forest land, agriculture land and lake bank) and processed. Isolated actinomycetes were screened by primary and secondary screening for antibiotic producers against test organisms like Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa (ATCC 27853) and Bacillus spp, E coli (ATCC 25922). This study isolated 27 actinomycetes in total, using the soil samples through spread plating on Starch Casein Agar (SCA) and by serial dilutio...
The aim of the present study was to isolate and evaluate the antimicrobial potential of soil actinomycetes of Kashmir Himalayas. The secondary metabolites of actinomycetes are the prominent source of antibiotics. A total of 121 morphologically different actinomycete strains were isolated and screened for antimicrobial activity against various human pathogens. The ethyl acetate extract of fermented broth an actinomycete strain, identified as Streptomyces pratensis exhibited significant antimicrobial activity against Staphylococcus aureus ATCC 29213 with MIC 0.25 mg/ml and Mycobacterium tuberculosis Strain H37Rv with MIC 0.062 mg/ml. The strain S. pratensis IIIM06 was grown on large scale and their broth was extracted with ethyl acetate. The extract was subjected to various chromatography techniques which led to the isolation of four compounds whose structures were established as actinomycin C1, actinomycin C2, actinomycin C3 and actiphenol on the basis of spectral data analysis. Actinomycin C1, C2 and C3 exhibited potent antimicrobial activity against S. aureus as well as M. tuberculosis. The isolated indigenous acti-nomycetes exhibited good antibacterial activity and the study reveals that IIIM06 is a promising strain and could be of great potential for industrial applications.
Screening of Antibiotic Producing Actinomycetes for Antibiosis from Soil of Siraha, Nepal
Himalayan journal of science and technology, 2017
Actinomycetes are gram positive bacteria widely distributed in natural and man-made environments. They are found in large numbers in soils, fresh waters, lake, river bottoms, manures, composts, dust as well as on plant residues and food products. However, the diversity of actinomycetes that produce secondary metabolites can be determined by different physical, chemical and geographical factors (Ogunmwonyi et al., 2010). As there is increasing trends of antibiotic resistant microorganisms, the search for novel antibiotics is necessary. In this case, actinomycetes are the best common sources of novel antibiotics (Okami & Hotta, 1988). The diversity of terrestrial actinomycetes is of great signi cance in several areas of medical sciences, particularly in antibiotic production (Magarvey et al., 2004). Out of 22,500 biologically active compounds obtained from microbes, 45% are from actinomycetes (Berdy, 2005). Need of new antimicrobial agents is greater than before because of the emergence of new multidrug resistance in common pathogens, rapid emergence of new infections, and the use of multidrug resistant pathogens in bioterrorism (Spellberg et al., 2004). Antibiotic resistant bacteria have been great problems in the treatment ofi nfectious diseases which are still the second leading cause of death worldwide (WHO 2002 and Luzhetskyy et al., 2007). In the search for novel antibiotics, a study in Nepal revealed that twenty seven actinomycetes isolated from soil samples of Mount Everest region were reported to have antibacterial activity (Gurung et al., 2009). Likewise, One hundred and seventeen antibiotic producing actinomycetes were isolated from non-agricultural wasteland, alkaline soils and compost rich garden soil (Kumar et al., 2010). Twenty species of actinomycete were isolated from marine soil samples in which s three showed signi cant antimicrobial activity against Staphylococcus aureus Escherichia coli and (Kalyani et al., 2012). These all evidences show the abundant presence of actinomycetes having antimicrobial activity. In this regard, Terai (75-600 m), a warm region of Nepal, is of a signi cant interest. Sun radiations and seasonal variations create extreme environment which likely to harbor unusual microorganisms. This poorly studied habitation increases the chances of nding novel microorganisms. Therefore, the present study was undertaken to isolate and characterize antibacterial actinomycetes from soil samples of Siraha, a warm district of Terai, Nepal.
2019
Due to acquired resistance produced by micro-organism against all frequently used antimicrobial agents, there is a great need of newer antibiotics to fight against different microbes. To explore the unknown diversity of microorganisms, soil screening for antimicrobial agents are performed for the detection of newer antibiotics from Actinomycetes. Studies were carried out with isolation of total 65 actinomycetes from rhizospheric soil of plants. Isolation and characterization of antimicrobial agents producing actinomycetes was performed and after screening some of isolates were having antimicrobial activity. Among them isolate AIA29 from rhizospheric soil was further studied for their potential. In the present study soil samples have been collected from different regions of Rajasthan. Isolation of actinomycetes was done on Actinomycetes Isolation Agar (AIA) media. Gram staining, characterization of colony and extraction of bioactive compounds was done by solvent extraction method. An...
Isolation and screening of soil Actinomycetes as source of antibiotics active against bacteria
Inter. J. Microbiol. …, 2010
A total of 117 actinomycetes strains were isolated from the wasteland alkaline and garden soil samples of the Ghaziabad and screened for their anti-bacterial activity. They were evaluated for their inhibitory activities on four test microorganisms. Fifteen actinomycetes isolate which exhibited antimicrobial activity against at least two of the test organisms and were characterized by conventional methods. The cultural characteristics of isolates were also studies in different culture media. The results indicated that six isolates were highly active against Staphylococcus aureus strains. Seven isolates were highly active with an inhibition zone more than 20 mm in diameter. Most of the isolates inhibited growth of the Gram negative bacteria tested. All the antibiotic producing actinomycetes were isolated at different temperatures from non agricultural wasteland alkaline soil and compost rich garden soil. Fifteen isolates showed activity against bacteria in which most of them from wasteland alkaline soil where the less interference by human for agriculture or other purpose. These microorganisms may have capability to produce some of the most important medicines ever developed.
— Soil, actinomycetes plays an important role in pharmaceutical industries because they produce valuable natural antibiotics to treat chronic diseases in humans. In the present study, the actinomycetes were isolated from four different sample sites of Western Ghats. Totally twelve isolates were selected and screened for antibiotic activity using coliforms. Among the twelve isolates, two were found to effective against coliforms and these isolates were obtained from soil of Courtallum. Antibiotic sensitivity of the twelve isolates revealed varying performance. The two actinomycetes isolates of Courtallum have a potential to be included in researches of new preparations with characterization of the bioactive compounds.
The purpose of this study was to isolate the antibiotic producing actinomycetes from soil from different parts of Kathmandu valley and to access their antimicrobial activity against different pathogenic bacteria like Staphylococcus aureus (ATCC 25923), Escherichia coli(ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). This study also focuses on investigating the distribution of the antibiotic producing actinomycetes according to the texture and cultivation status of soil. Fifty four colonies of actinomycetes were isolated from the soil collected from five different parts of Kathmandu valley in the standard medium out of which 20 colonies produced antibiotics; all of them belonged to genus Streptomyces. One of the isolated colony largely inhibited the growth of E. coli and Staphylococcus aureus with zone of inhibition more than 20 mm for both. Antibiotic from this colony was produced by submerged fermentation and the minimum inhibitory concentration of the crude antibiotic was determined.
International Journal of Pharmacy and Pharmaceutical Sciences, 2018
Objective: Isolation and characterization study of actinomycetes from soil near medicinal plants in saurashtra region of Gujarat which can produce antimicrobial compounds. Methods: Present study 11 different medicinal plants 3 regions of saurashtra selected. Microflora part of soil was used. Different media used for isolation. Microscopic methods used for Study of colony characteristics, arial and vegetative mycelium color and morphological examination of isolates. Primary screening was done by using cross streak method while for secondary screening well diffusion techniques with thin layer chromatography and bioautography. Antimicrobial activity of most potent isolates check against test organisms. Different biochemical methods used for characterization of the most active isolate. Results: It was found that out of 66 actinomycetes isolates 45 (68.2 %) actinomycetes isolates were showing activity against test microbes. In the primary screening, 15 actinomycets isolates showed good antimicrobial activity. Out of these 15 actinomycetes isolates 5 isolates GOS1, JOS1, GOS3, GTF1 and JTF1 showing a broad spectrum of activity against microorganisms. It was found that out of this 5 isolates GOS1 was most potent antagonistic actinomycetes. Characterizations, as well as optimization studies of actinomycetes, isolates GOS1 which may use to produce new and useful antimicrobial compounds and other metabolites. Conclusion: It was concluded that actinomycetes isolates from different soil samples nearby medicinal plant area of saurashtra region are a good source to produce many useful antimicrobial compounds and other metabolites.
Isolation and screening of antibiotic producing actinomycetes from soilsinManong, Perak, Malaysia
Der Pharmacia Lettre, 2016
The present study focuses on isolation of actinomycetes from the soil in Ulu Kenas forests of Manong, located in the northern part of Perak state in Malaysia and screening for antibacterial activity. All the isolates were screened for their antibacterial activity using Kirby-Bauer (KB) method against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnemoniae and Staphylococcus aureus. Out of the ten isolated actinomycetes, seven, namely B, D, E, F, H, I and J showed antimicrobial activity against selected bacteria. However, isolates E showed maximum inhibition against P. aeruginosa and the isolates I and J against K. pneumonia with highest scores. The findings of the study may be helpful to the future investigators in identifying alternative and new bioactive secondary metabolites like antibiotics to treat resistant human pathogens.
Screening of Actinomycetes from Soil for Antibacterial Activity
2020
Actinomycetes are Gram positive, free living saprophytes which are distributed in soil as one of the major populations and are primary source of antibiotics. This study was carried out with a quest to isolate actinomycetes from soil samples of different places and assess their antibacterial activity. Isolation of actinomycetes was carried out by serial dilution of soil sample followed by spread plate method. The antimicrobial extract was extracted using ethyl acetate. Assessment of antimicrobial activity was performed by using Agar cup plate assay against test organisms (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Salmonella paratyphi, Bacillus subtilis, Staphylococcus aureus). Antibacterial activity was tested against Methicillin Sensitive Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus in the isolates having effective inhibitory activity against Staphylococcus aureus. From 15 soil samples of 12 different locations, 121 a...