Recovery of succinic acid from fermentation broth by forward osmosis-assisted crystallization process (original) (raw)
Related papers
Purification of High Value Succinic Acid from Biomass Fermentation Broth Via Nanofiltration
Indian Journal of Science and Technology, 2016
Background/Objectives: Succinic acid purification from biomass fermentation experiences difficulty due to similar properties of acids presence. Thus purification work is conducted using nanofiltration (NF) membranes, and further characterizing its filtration performances. Methods/Statistical Analysis: The membranes used were NF1 and NF2 from AmforInc and NF270 from Filmtec. The investigated filtration performance parameters were different acids concentration and operating pressure of 5, 10, 20 and 50 g/L and 2, 5, 10 and 15 bar, respectively. Findings: From the results, NF270 showed the most efficient in purifying the succinic acid at 20 g/L and 50 g/L, which were 76% and 64.4% purification, respectively from 33% concentration in the feed solution. Meanwhile for different pressure, NF2 shows the highest purification percentages which were 82% and 81% at the respective 5 and 10 bar followed by NF270 membrane at 68% and 67% at the respective 5 and 10 bar. The characterization of the membranes using DSC, FTIR, contact angle and SEM method shows that there was no fouling on the membranes observed after the filtration when there were no differences shown on the analysis results. This indicates that the range of pressure and concentration used in this study are suitable to perform the purification. Contact angle has found to influence the percentage of purification of each membrane where the less hydrophilic membrane shows the highest purification percentage. Application/Improvements: Thus NF270 and NF2 membranes were considered as the best candidate for purifying the succinic acid from fermentation broth.
Separation and Purification Technology
Spent sulphite liquor produced as side stream from sulphite pulping of Eucalyptus globulus hardwood could be used for the separation of lignosulphonates by nanofiltration in the retentate stream and succinic acid production via fermentation of the permeate stream by Actinobacillus succinogenes or Basfia succiniciproducens. The potential integration of this process in conventional pulp mills towards the development of a novel biorefinery is dependent on the efficient downstream separation of succinic acid crystals at high yield and purity. This study focuses on the evaluation of five downstream separation processes, namely calcium precipitation, direct crystallisation using acidification or cation-exchange resins, salting-out and reactive extraction, for the purification of succinic acid from crude fermentation broths. Reactive extraction using trioctylamine in 1-hexanol and direct crystallisation coupled with cation-exchange resins led to succinic acid recovery yields of 73% and 79%, respectively. 1 H-NMR analysis showed that these downstream separation processes led to succinic acid crystal purities of ca 98.5% for reactive extraction and higher than 99% for the direct crystallisation method coupled with cation-exchange resins with no detectable acetic acid content when re-crystallisation was employed. It has been demonstrated that succinic acid produced via fermentation using side streams from pulp and paper mills could be separated at high purity and yield from crude fermentation broths rendering feasible its utilisation for poly(butylene succinate) production.
Downstream processing of biotechnological produced succinic acid
Applied Microbiology and Biotechnology, 2012
Succinic acid is a promising chemical which has a wide range of applications and can be biologically produced. The separation of succinic acid from fermentation broth makes more than 50 % of the total costs in their microbial production. This review summarizes the present state of methods studied for the recovery and purification of biologically produced succinate. Previous studies on the separation of succinic acid primarily include direct crystallization, precipitation, membrane separation, extraction, chromatography, and in situ separation. No single method has proved to be simple and efficient, and improvements are especially needed with regard to yield, purity, and energy consumption. It is argued that separation technologies coupled with upstream technology, in situ product removal, and biorefining strategy deserve more attentions in the future.
Green Chem., 2008
A novel alternative methodology (direct crystallisation) to the traditional calcium precipitation to obtain succinic acid (SA) from defined and wheat-based fermentation broths is reported. SA crystals were successfully recovered from fermentation broths (FB) using this method. A higher SA crystal purity (95%) and yield (70%) were obtained in the direct crystallisation method compared to a slightly modified traditional calcium precipitation method (90% and 24%, respectively). Chemical transformations (e.g. esterifications) to high-added value derivatives of both recovered SA crystals were then investigated using a range of solid acids including our acidic tunable mesoporous carbonaceous materials denoted as Starbon R acids. Results showed that SA crystals could be successfully converted into mono-and diesters in high yields and selectivities employing solid acids regardless of the reaction conditions. The order of reactivity was found to be: pure SA crystals > SA crystals from defined FB > FB SA crystals. Results demonstrate that SA can be effectively purified from actual fermentation broths, showing the importance of integrating the fermentation and downstream processing to optimise the fermentative production of SA and its chemical transformations to produce high-added value derivatives.
An integrated membrane process that consists of nanofiltration (NF) and vapor permeation (VP) was employed as a series of purification process for fermentation-derived succinic acid. Separation performance of a ceramic NF membrane was examined for both model solutions and fermentation broth. Rejection of organic acids was investigated for model solutions as a function of feed pressure, feed concentration, and pH. For fermentation broth, the NF showed its usefulness for protein and color removal rather than separation among organic acids. The esterification reactions of succinic acid with ethanol were initially investigated using model solutions. The yield of diethyl succinate (DES) was the function of initial reactant ratio whilst the operating temperature played an important role in productivity. Realistic purification was performed with NF-treated fermentation broth using Actinobacillus succinogenes ATTC 55618 as the succinic acid producer. The yield and volumetric productivity of DES strongly depended on the dehydration rate. Experimental results showed that most succinic acid was converted into DES at the end of the VP-assisted esterification reaction. After fractionation and hydrolysis, a high purity of succinic acid was obtained.
Separation Science and Technology, 2015
This work aims to study how to remove the organic acid by-products from simulated fermentation broth containing succinic acid by re~ctive extraction. Model solutions including single-, binary-, ternary-, and quaternary-solute solutions were used. The broths were reactively extracted using 0.25 mol TOA/kg l-octanol under pH of 2.45-6.0. The extracted broths were then distillated under vacuum-0.017 MPa with operating temperature between 45 and 65°(, Finally the distilled broths were crystallized at 4°C and pH of 2.0. The results showed that the purity and yield of succinic acid of 99.10% and 30.25%, respectively, were obtained.
Extraction of succinic acid from real fermentation broth via emulsion liquid membrane
2018
Succinic acid is listed as one of the twelve building block chemicals based on the ease of production through a biotechnological approach and potential to derive various chemicals. The application of bio-based succinic acid is still limited due to high downstream processing costs. One of the potential methods to recover succinic acid is emulsion liquid membrane (ELM). The ELM system consists of three main liquid phases; external feed, membrane, and internal. In this study, the membrane phase was prepared by dissolving Amberlite LA2 as a carrier, sorbitan monooleate (Span 80) and polyoxyethylenesorbitan monooleate (Tween 80) as surfactants in commercial grade palm oil, while the internal phase comprised of sodium carbonate solution, Na 2 CO 3 . The influence of emulsifying time, agitation speed, and agitation time on the water-in-oil-in-water (W/O/W) emulsion stability were studied. The most stable condition was implemented on various external phase concentrations to study the extrac...
Extraction of Succinic Acid from Real Fermentation Broth by Using Emulsion Liquid Membrane Process
2018
Succinic acid is listed as one of the twelve building block chemicals based on the ease of production through a biotechnological approach and potential to derive various chemicals. The application of bio-based succinic acid is still limited due to high downstream processing costs. One of the potential methods to recover succinic acid is emulsion liquid membrane (ELM). The ELM system consists of three main liquid phases; external feed, membrane, and internal. In this study, the membrane phase was prepared by dissolving Amberlite LA2 as a carrier, sorbitan monooleate (Span 80) and polyoxyethylenesorbitan monooleate (Tween 80) as surfactants in commercial grade palm oil, while the internal phase comprised of sodium carbonate solution, Na2CO3. The influence of emulsifying time, agitation speed, and agitation time on the water-in-oil-in-water (W/O/W) emulsion stability were studied. The most stable condition was implemented on various external phase concentrations to study the extraction...
Separation Science and Technology
Experiments were performed for comparing and selecting the most appropriate precipitation strategy for succinic acid (SA) recovery from carob pod extract fermentation broths. The performances of three downstream optionsemploying calcium hydroxide, magnesium hydroxide or ammoniawere investigated and compared from operational, techno-economical and sustainability viewpoints. The highest SA recovery (84.3%) was obtained with ammonia, whereas the calcium-based treatment proved inadequate. Sustainability indicators favoured magnesium-based treatment, closely followed by the ammonia-based one. A preliminary economic analysis favours magnesium-based treatment, which appears to be the most feasible option. However, materials recycling and possible by-products commercialization could well position also ammonia-based treatment.