Downstream processing of biotechnological produced succinic acid (original) (raw)
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Integrated separation process for isolation and purification of biosuccinic acid
Biotechnology Progress, 2011
Biotechnologically produced succinic acid has the potential to displace maleic acid and its uses. Therefore, it is of high interest for the chemical, pharmaceutical, and food industry. In addition to optimized production strains and fermentation processes, an efficient separation of succinic acid from the aqueous fermentation broth is indispensable to compete with the current petrochemical production of succinic acid. Isolation and purification of succinic acid from an Escherichia coli fermentation broth were studied with two amine‐based reactive extraction systems: (i) trihexylamine in 1‐octanol and (ii) diisooctylamine and dihexylamine in a mixture of 1‐octanol and 1‐hexanol. Back extraction of succinic acid from the organic phase was carried out using an aqueous trimethylamine solution. The trimethylammonium succinate generated after back extraction was split with an evaporation‐based crystallization. The focus was on process integration, for example, reuse of the applied amines ...
Biotechnology of succinic acid production and markets for derived industrial products
Applied Microbiology and Biotechnology, 1999
Succinic acid, derived from fermentation of agricultural carbohydrates, has a specialty chemical market in industries producing food and pharmaceutical products, surfactants and detergents, green solvents and biodegradable plastics, and ingredients to stimulate animal and plant growth. As a carbon-intermediate chemical, fermentation-derived succinate has the potential to supply over 2.7´10 8 kg industrial products/ year including: 1,4-butanediol, tetrahydrofuran, c-butyrolactone, adipic acid, n-methylpyrrolidone and linear aliphatic esters. Succinate yields as high as 110 g/l have been achieved from glucose by the newly discovered rumen organism Actinobacillus succinogenes. Succinate fermentation is a novel process because the greenhouse gas CO 2 is ®xed into succinate during glucose fermentation. New developments in end-product recovery technology, including water-splitting electrodialysis and liquid/liquid extraction have lowered the cost of succinic acid production to U.
New reactive extraction systems for separation of bio-succinic acid
Bioprocess and Biosystems Engineering, 2011
Biotechnologically produced succinic acid has the potential to displace maleic acid and its uses and to become an important feedstock for the chemical industry. In addition to optimized production strains and fermentation processes, an efficient separation of succinic acid from the aqueous fermentation broth is indispensable to compete with the current petrochemical production processes. In this context, high molecular weight amines are known to be effective extractants for organic acids. For this reason, as a first step of isolation and purification, the reactive extraction of succinic acid was studied by mixing aqueous succinic acid solutions with 448 different amine-solvent mixtures as extraction agents (mixer-settler studies). The extraction agents consist either of one amine and one solvent (208 reactive extraction systems) or two amines and two solvents (240 reactive extraction systems). Maximum extraction yields of succinic acid from an aqueous solution with 423 mM succinic acid at pH 2.0 were obtained with more than 95% yield with trihexylamine solved in 1-octanol or with dihexylamine and diisooctylamine solved in 1-octanol and 1-hexanol. Applying these optimized reactive extraction systems with Escherichia coli fermentation broth resulted in extraction yields of 78-85% due to the increased ionic strength of the fermentation supernatant and the coextraction of other organic acids (e.g., lactic acid and acetic acid), which represent typical fermentation byproducts.
Biotechnological production of succinic acid: current state and perspectives
Biofuels, Bioproducts and Biorefining, 2012
Succinic acid has multiple practical applications (e.g. synthesis of 1,4-butanediol, tetrahydrofuran, gamma-butyrolactone, and as a monomer of some biodegradable polymers). Bio-based succinic acid is a potential substitute for current petrochemical production. Facing a shortage of crude oil supply and sharply rising oil prices, biological production of succinic acid from abundant and available biomass has become a topic of worldwide interest. Although great progress has been made in recent decades, much needs to be developed further in order to achieve economic viability. This paper reviews developments in technology and updates research progress of biosuccinate production, including pathways, microorganisms , culture conditions, as well as integrated production with other high-value-added products. Finally, strategies are proposed for successful commercialization of fermentative succinic acid production.
Separation and Purification Technology
Spent sulphite liquor produced as side stream from sulphite pulping of Eucalyptus globulus hardwood could be used for the separation of lignosulphonates by nanofiltration in the retentate stream and succinic acid production via fermentation of the permeate stream by Actinobacillus succinogenes or Basfia succiniciproducens. The potential integration of this process in conventional pulp mills towards the development of a novel biorefinery is dependent on the efficient downstream separation of succinic acid crystals at high yield and purity. This study focuses on the evaluation of five downstream separation processes, namely calcium precipitation, direct crystallisation using acidification or cation-exchange resins, salting-out and reactive extraction, for the purification of succinic acid from crude fermentation broths. Reactive extraction using trioctylamine in 1-hexanol and direct crystallisation coupled with cation-exchange resins led to succinic acid recovery yields of 73% and 79%, respectively. 1 H-NMR analysis showed that these downstream separation processes led to succinic acid crystal purities of ca 98.5% for reactive extraction and higher than 99% for the direct crystallisation method coupled with cation-exchange resins with no detectable acetic acid content when re-crystallisation was employed. It has been demonstrated that succinic acid produced via fermentation using side streams from pulp and paper mills could be separated at high purity and yield from crude fermentation broths rendering feasible its utilisation for poly(butylene succinate) production.
Production of succinic acid by bacterial fermentation
Enzyme and Microbial Technology, 2006
Succinic acid produced by various microorganisms can be used as a precursor of many industrially important chemicals in food, chemical and pharmaceutical industries. The assessment of raw material cost and the estimation of the potential market size clearly indicate that the current petroleum-based succinic acid process will be replaced by the fermentative succinic acid production system in the foreseeable future. This paper reviews processes for fermentative succinic acid production, especially focusing on the use of several promising succinic acid producers including Actinobacillus succinogenes, Anaerobiospirillum succiniciproducens, Mannheimia succiniciproducens and recombinant Escherichia coli. Processes for the recovery of succinic acid from fermentation broth are also reviewed briefly. Finally, we suggest further works required to improve the strain performance suitable for successful commercialization of fermentative succinic acid production.
Theoretical and Experimental Study of Biobased Succinic Acid Production
2017
Biomass based succinic acid is gaining increasing interest as a potential platform chemical for replacing a large petroleum-based bulk chemical market. Biomass as a renewable resource has proved the economic and sustainable potential to produce succinic acid by fermentation method. Biobased succinic acid has yet faced with the challenge of becoming competitive with petrochemical method because of its higher production cost. To lower the production cost, extensive research efforts have been undertaken in upstream technology that involves strain development via metabolic engineering, and downstream technology that aims to improve efficiency of purification method. Many research studies have focused on either one of two technological areas, with little interest on interaction between them. This present work integrates the processing steps from upstream and downstream technologies using a systematic approach and presents an optimal production pathway from a large number of possible process configurations. The development of such a process pathway involves selection of bioproducts, feedstock, pre-treatment technology, microorganism and product separation method. Performance criteria such as titre, rate, yield and minimum production cost, express the optimality of production pathway. Optimization study indicates that succinic acid seems to be the most promising bioproduct among all other bioproducts. Corn stover is the suitable feedstock to produce succinic acid. Based on the findings from optimization study, experimental work was performed with an aim of achieving better performance criteria than it is reported in literature. This work selected corn stover as feedstock, and a bacterium called, Basfia succiniciproducens for converting corn stover-derived glucose into succinic acid. To date, no deliberate experiment has been done on this bacterium to improve succinic acid production, despite its promising features. Highest succinic acid yield of 18 g/100g total
Journal of Membrane Science, 2019
In this study, osmotically driven forward osmosis (FO) was employed prior to crystallization process in the downstream recovery of bio-based succinic acid. The fermentation broth containing succinic acid was initially pretreated using activated carbon. Powdered activated carbon (PAC) showed its effectiveness for glucose, formic acid, and color removal while succinic acid concentration remained unaffected. The untreated and treated fermentation broths were then concentrated using the FO process. FO exhibited a remarkable enhancement of concentration factor (CF) by 3.9-fold for the treated broth, thus resulting in a final succinic acid concentration of 111.26 g/L. By contrast, higher flux loss and lower CF were observed for untreated broth, mainly due to the adverse effect of severe membrane fouling and cake layer formation. Succinic acid crystals were then successfully recovered from the FO-concentrated broth in the final crystallization step. The purity and yield of succinic acid crystals were 90.52% and 67.09%, respectively for treated broth. This work demonstrated the development of a feasible FO-crystallization process for the downstream recovery of bio-based succinic acid. The findings have important implications for practical applications of FO technology in the bioprocess industries.
Production of Succinic Acid Through Anaerobic Screening of Related Microbial Strain
2000
Fermentation derived succinic acid is an economic process for supplying the existing succinic acid especially in chemical market. Production of succinic acid by fermentation can generate significant new markets for agricultural carbohydrates. The present work was undertaken with the objective to investigate a novel and simple fermentation process of succinic acid production at low cost from the renewable sources. Out of one hundred and two DBRL; isolates which are used in the study, only five strains gives positive results under both aerobic and anaerobic condition for succinic acid production. When these one hundred and two isolates were tested in indicator medium without CaCO only nineteen strains gives positive results under anaerobic 3 condition. However, only eighteen strains showed positive results with CaCO under same condition and only 3 five strains showed positive results with and without CaCO under anaerobic condition. From the present study 3
Microbial succinic acid production: Natural versus metabolic engineered producers
Process Biochemistry, 2010
The increased consciousness for environmental issues and the depletion of mineral oil reserves led to the search for alternative energy sources but also for alternative biochemical processes. One of these chemicals that is identified to have great economical potential in a biobased economy is succinic acid. This chemical is a precursor for various high value-added derivatives which have application in the detergent/surfactant market, the ion chelator market, the food market and the pharmaceutical market. This review investigates the goals ...