Synergistic anticancer effect of the extracts from Polyalthia evecta caused apoptosis in human hepatoma (HepG2) cells (original) (raw)
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International Journal of Biosciences (IJB), 2020
Cancer presently exists as the major pathological state in developed and emergent nations equally which is characterized by the uncontrolled division of cells and is fatal. People prefer anticancer plant products to treat cancer due to the increased mortality related to undetected growth in addition to its toxic reactions to chemotherapy and radiotherapy. In recent years, eastern medicine provided a profitable substitute for allopathic medicine against cancer. There have been considerable researches on plants for treating cancer, and many of the plant products have been sold as anticancer medicine, depending on the conventional uses and experimental reports. The anticancer property of medicinal plants is due to their antioxidant activity which has been described in many reports. Thus, after isolation (by using chromatographic and crystallization techniques) and identification using LCMS spectra, IR, and NMR, many combinations of the active components can be prepared and should be further evaluated for their synergic effects. The development of a systemized dose and dosage procedure may perform an exceptional role in the cure of tumors. The rate at which cancer develops is a necessary and effective endeavor for the good health of human beings. Further investigations are required to analyze the mechanisms of anti-malignant action of active compounds from new plants and the use of standard herbal remedies.
Anticancer activity of plant leaves extract collected from a tribal region of India
3 Biotech, 2019
The goal of this research was to explore the preliminary anticancer properties of five plants namely Calotropis procera, Moringa oleifera, Millettia pinnata, Basela alba and Euphorbia neriifolia available in Jharkhand which is used for the medicinal purpose by local tribes. In the present study, plant leaves from five species were collected, dried and extracted with solvents of increasing polarity, followed by assessment of their cytotoxicity in A549 non-small-cell lung cancer cells. In the antimicrobial assay, the methanol extract of the M. pinnata leaves exhibited comparatively higher zone of inhibition of 0.7 ± 0.20 cm against a Salmonella typhi culture than the other extracts. M. pinnata leaves extract also displayed the maximum percentage inhibition in the DPPH, 83.97 ± 0.01 FRAP, 193.14 ± 3.01 mM assays. Furthermore, the cytotoxicity of the chloroform (37.45 ± 1.04) and ethyl acetate extracts (34.20 ± 0.81) of M. pinnata against A549 cells was found relatively higher with respect to another extract. In contrast, a study with the L132 normal epithelial lung cell line revealed less toxicity from the chloroform extract (0.33 ± 0.19) compared to the ethyl acetate extract (6.65 ± 0.59). Based on these findings, phytochemical investigation on chloroform and ethyl acetate extract of M. pinnata was performed using UPLC-ESI-MS/MS analysis revealing the presence of β-sitosterol, lanceolatin B, karanjin, and stigmasterol. Congruently, a complete phytochemical and cytotoxic investigation of the M. pinnata extract constituents might infer the potency of this extract/s as anticancer, antioxidant and antimicrobial agents. Keywords Anticancer • Antioxidant assay • Apoptosis • Mass spectroscopy Abbreviations DPPH 1,1-Diphenyl-2-picrilhydrazyl AO/EtBr Acridine orange/ethidium bromide DAPI 4′,6-Diamidino-2-phenylindole, dihydro chloride FRAP Ferric reducing ability of plasma h Hour/hours m/z Mass-to-charge ratio Electronic supplementary material The online version of this article (
Evaluation of some plant extracts for standardization and anticancer activity
Indian journal of traditional knowledge
In recent times, the trend in cancer research is shifting towards identifying new medicines from natural resources for management of cancer. Medicinal plants such as Sthauneyaka (Taxus baccata L.) and compound formulations like Triphala ghrita, Khadirarista, Madhusnuhi rasayana, Maha triphaladya ghrita, Panchatikta guggulu ghrita are indicated in the Ayurvedic texts for management of cancer/ tumour. The anti-proliferative activities of hydro-alcoholic extracts of some standardized plant materials were screened against a panel of 14 human cancer cell lines representing different tissues (lung, pancreas, colon, cervix, oral, bladder, prostate, breast, leukaemia, etc.) through Sulforhodamine-B (SRB) assay. The findings revealed that Cedrus deodara (Roxb.) ex Lamb. and Berberis aristata (Roxb.) ex DC. have maximum anticancer activity against 3 cell lines while Withania somnifera Dunal. showed activity against two cell lines. In addition to these, Picrorhiza kurroa Royle ex Benth. and Pi...
Anticancer activity of Medicinal plant extract-A re view
2010
Traditional medicine has a long history of serving peoples all over the world. India is without doubt a herbal hub. Medicin al plants that are native to India and their use in various traditiona l systems of medicine are indeed awe-inspiring. The ethnobotany and ubiquitous plants provide a rich resource for Natural drug research a nd development. In recent years, the use of traditional medicine infor mation on plant research received considerable interest. The medici nal plants contain several phytochemicals such as vitamins, carotenoid s, terpenoids, flavonoids, polyphenols, alkaloids, tannins, saponi ns, enzymes, minerals etc. These phytochemicals possess antioxid ant activities, which, prevent or can be used in the treatment of m any diseases, including cancer. There are the several medicinal p lants all over the world, including India, which are being used tradit ionally for the prevention and treatment of cancer. The present pap er is a comprehensive review of different literat...
Antitumor and antioxidant activity of Polyalthia longifolia stem bark ethanol extract
Pharmaceutical …, 2010
In the present study, the ethanol extract of stem bark of Polyalthia longifolia Benth. and Hook (Annonaceae) was screened for its in vitro and in vivo antitumor activity. In vitro cytotoxicity of P. longifolia extract was assessed in murine cancer cells and in human cancer cells by Trypan blue exclusion assay and MTT assay, respectively. P. longifolia extract showed concentration-dependent cytotoxicity in Ehrlich's ascites carcinoma (EAC) and Dalton's ascites lymphoma (DLA) cells with IC 50 values of 45.77 and 52.52 µg/mL, respectively. In the MTT assay, the IC 50 values of P. longifolia extract against HeLa and MCF-7 cells were 25.24 and 50.49 µg/mL, respectively. In vivo antitumor activity against Ehrlich's ascites tumor and Dalton's solid tumor models was assessed by administering 50 and 100 mg/kg of P. longifolia extract, i.p., for 7 consecutive days. P. longifolia extract, at a dose of 100 mg/kg, significantly enhanced mean survival time (MST) and marginally improved hematological parameters when compared to EAC control mice. And the same dose significantly reduced the tumor volume as compared to control DLA inoculated mice. Positive control, cisplatin (3.5 mg/kg, i.p., single dose), significantly enhanced MST and improved hematological parameters when compared to EAC and significantly reduced the tumor volume when compared to DLA control. In vitro antioxidant potential of P. longifolia extract was also determined owing to the role of reactive oxygen species in tumor initiation and progression. P. longifolia extract scavenged DPPH radicals, reduced ferric ions and inhibited lipid peroxidation with IC 50 values of 18.14, 155.41 and 73.33 µg/mL, respectively.
Journal of Advanced Scientific Research
In the present study, phytochemical constituents of medicinal plants were evaluated from their ethanolic extracts, such as Andrographis paniculata, Centella asiatica, Psidium guajava and Solanum trilobatum. The extracts were subjected to a qualitative phytochemical screening using a standard procedure. Our study has identified phytocompounds present in leaf extracts of Andrographis paniculata, Centella asiatica, Psidium guajava and Solanum trilobatum using IR and GC-MS spectroscopy. An anticancer activity was performed on each of these extracts using two human cancer cell lines, a colon cancer cell line (HCT116) and a cervical cancer cell line (HeLa). Despite the four medicinal plants extracts having comparable anticancer activity, the ethanolic extracts of Andrographis paniculata and Solanum trilobatum show higher anticancer activity than Centella asiatica and Psidium guajava. The Andrographis paniculata (IC50=27.54 μg/ml HeLa and 30.19 μg/ml HCT116) and Solanum trilobatum (IC50=32...
Anticancer Activity of Five Traditionally Used Medicinal Plants’ Extracts
Journal of Cancer Research Updates, 2014
Natural products play a critical role in cancer prevention and therapy today. There are numbers of anticancer agents from natural products used in the clinic. Fighting cancers with novel natural products, especially those extracted from plants, is a potential strategy to develop new anticancer drugs..In the following study, various extracts of well known medicinal plants named Holoptelea integrifolia (F), Operculina turpethum (R), Cardiospermem halicacabum L (S), Dilonix regia (F), Sesbania grandifora seed have been studied for evaluating their anticancer activity. Our data showed that the cytotoxic activity of Operculina turpethum (R) ethanolic extract was relative high for all 6 cancer cell lines as compared to other extracts. The active compound and anticancer mechanism of these extracts are worth investigating in the future.
In-Vitro Screening of Some Plant Extracts for Their Potential Anticancer Activity
African Journal of Traditional, Complementary and Alternative Medicines
Background: Natural products have been shown to be reliable sources of anticancer medicines although there is still a consistent demand for new therapeutic natural products for cancer treatment with minimal side-effects. Materials and Methods: In this study, six plant extracts (Grevillea robusta; Euphorbia millii; Euphorbia royleana; Aloe grandidentata; Bauhinia corniculate; and Cassia fistula) were screened for the presence of phytochemical metabolites as saponins, tannins, cardiac glycosides, alkaloids, flavonoids, anthraquinones and sterols, using qualitative tests. Antiproliferative screening assay was performed on a panel of three cancer cell-lines (HepG-2, HCT-116 and MCF-7) using MTT assay, and cytotoxicity was determined using WI-38 human fibroblast cell-line. Results: Some plant extracts reduced cellular growth for the selected cancerous cell-lines. For example, E. royleana and A. grandidentata extracts reduced HepG-2 cellular growth with IC50 of 0.42 and 0.53µg/mL, respectively. Moreover, A. grandidentata and C. fistula reduced cellular growth of MCF-7 with IC50 of 0.37 and 0.67 µg/mL, respectively. Conclusion: E. royleana, A. grandidentata and C. fistula showed significant anti-proliferative activity against HepG-2 and MCF-7 cell-lines with non-cytotoxic nature. This suggests their potential role as anticancer agents against these types of cancer. The presence of flavonoids, sterols and anthraquinones may suggest their enhanced anti-proliferative activities. Therefore, this study has shed light on the possible use of these extracts as potential sources of natural products-based therapy for cancer.