Antibacterial Effect of Zinc Oxide Nanoparticles on Standard Strains and Isolates of Pseudomonas Aeruginosa and Staphylococcus Aureus (original) (raw)
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Nanoparticles (NPs) are increasingly recognised for their utility in biological applications including nanomedicine and food safety. The present study investigated the antibacterial activity of zinc oxide (ZnO) when tested against the Gram-negative bacteria Escherichia coli as well as the Gram-positive bacterium Staphylococcus aureus, and the effect was more pronounced with the Gram-positive than with the Gram-negative bacteria. ZnO NPs also exhibited a preferential ability to suppress growth of E. coli and S. aureus in milk. This study suggested that the application of ZnO NPs as antibacterial agents in food systems and medicine may be effective at inhibiting certain pathogens.
International JOURNAL OF HEALTH SCIENCE, 2012
In the emerging issue of increased mult i-resistant properties in food borne pathogens, zinc o xide (Zn O) and nano-particle zinc o xide (nano-ZnO) are being used increasingly as antimicrobial agents. Thus, the min imu m inhibitory concentration (MIC) and minimu m bactericidal concentration (M BC) of nano -ZnO towards pathogens microbes Bacillus subtilus NRRL B-543, Bacillus megaterium ATCC 25848, Staphylococcus aureus; NRRL B-313, Sarcina lutea ATCC27853, Escherichia coli; NRRL B-210, Pseudomonas aeruginosa NRRL B23 27853, Klebsiella pneumoniae ATCC 27736, proteus vulgaris NRRL B-123, Candida albicans NRRL Y-477 and Aspergillus niger NRRL-3 were examined in this study.
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Journal of Applied Biotechnology Reports, 2018
Introduction: Recent increases in microbial resistance to multiple antibiotics have led to the emergence of more economical methods for producing nanoparticles with physical, chemical effects and limited resistance. The aim of this research was to study zinc oxide (ZnO) nanoparticles synthesis and antibacterial properties against some gram-negative and gram-positive bacteria. Material and Methods: In this study, ZnO nanoparticle was synthesized using ultrasonic method and bioassayed on 10 human pathogenic bacteria by agar well diffusion method. In addition, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as well. Antibiotic resistance pattern of bacteria was determined to 9 antibiotics: gentamicin, ampicillin, nalidixic acid, amoxicillin, amikacin, ciprofloxacin, co-trimoxazole, norfloxacin and cephalexin by disc diffusion assay. Results: The nanoparticles were synthesized with suitable morphology and distribution. All gram-positive and gram-negative bacteria were inhibited at the low concentration of ZnO nanoparticles most bacteria had resistance to antibiotics. Conclusions: The findings suggest that the ZnO nanoparticles have potential applications as antibacterial compounds and their mechanism of action is dependent upon composition and surface modifications.
2016
Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics. Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria. The aim of this study is to investigate the prevalence of resistance to vancomycin in clinical isolates of S. aureus as well as the antimicrobial effects of zinc oxide nanoparticles on them. In this study, 70 samples of wound, boil, abscess and urine were isolated.Staphylococcus aureus strains resistant to vancomycin were then identified through routine laboratory tests using Broth Micro dilution test. The antibacterial effect of ZnO nanoparticles (20 nanometer) was investigated at concentrations of 100, 50, 25, 12.5 and 6.25 mg/ml using agar well diffusion method over strains resistant to vancomycin. From the total of 70 samples, 30 sampl...
Synthesis, characterization and evaluation of antibacterial efficacy of zinc oxide nanoparticles
Objective: Objective of the study was to synthesize and characterize Zinc oxide (ZnO) nanoparticles (NP), and to evaluate their application on some bacterial strains. Methods: ZnO NP was synthesized by chemical methods. Then decomposed by using conventional heating process. The detailed characterization of the nanoparticles was performed using FT-IR, UV-Vis spectroscopy, X-Ray Diffraction analysis and XRF. From the analysis of XRD pattern, UV-VIS spectroscopy and XRF, the formation of nanoparticles was confirmed. Antibacterial assay of synthesized ZnO NP was carried out both in liquid and solid growth medium against a gram positive (Staphylococcus aureus) and a gram negative (Escherichia coli) bacteria using disc diffusion assay method. Effect of antibacterial activity was observed by zone of inhibition around the antibiotic discs of ZnO NP. Results: ZnO NP was characterized by the different spectral analysis of the synthesized product. ZnO NPs reveal good antibacterial activity against S. aureus and E. coli. Kinetic studies were conducted on growth bacteria by loading ZnO NP to S. aureus and E. coli with this concentration to study the kinetic of growth behavior which showed that NP produced toxicity on both bacteria and therefore the growth was inhibited. Conclusions: The inhibition of growth of the organisms by ZnO nanoparticles suggests that it could potentially be used as an effective antibacterial agent and as well can be used in the protection of agricultural and food safety. Future studies may be aimed at the further evaluation to establish the nanoparticles as potential antimicrobial agent.
International Journal of Molecular and Clinical Microbiology, 2016
Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics.Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria.The aim of this study isto investigate the prevalence of resistance to vancomycin in clinical isolates of Staphylococcus aureus as well as theantimicrobial effects of zinc oxidenanoparticles on them. In this study, 70 samples of wound, boil, abscess and urine were isolated.Staphylococcus aureus strains resistant to vancomycin were then identified through routine laboratory tests using Broth Microdilution test. The antibacterial effect of ZnO nanoparticles(20 nanometer) was investigated at concentrations of 100,50,25,12.5 and 6.25 mg/ml using Agar well diffusion method over strains resistant to vancomycin. From the total of 70 samples, 30 sam...